The molecular mechanism of n-butanol extracts of Panax notoginseng on RANKL-induced osteoclastogenesis in RAW264.7 cells

2014 ◽  
Author(s):  
Hyo-In Hwang ◽  
Young-Joo Jang ◽  
Seon-Yle Ko
Keyword(s):  
Molecular Mechanism ◽  
Panax Notoginseng ◽  
Raw264.7 Cells

Panax notoginseng Inhibits Tumor Growth through Activating Macrophage to M1 Polarization

2018 ◽  
Vol 46 (06) ◽  
pp. 1369-1385 ◽  
Author(s):  
Bosung Kim ◽  
Eun-Yeong Kim ◽  
Eun-Ji Lee ◽  
Jung Ho Han ◽  
Chung-Hwan Kwak ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Culture Media ◽  
Tumor Model ◽  
Annexin V ◽  
Panax Notoginseng ◽  
Raw264.7 Cells ◽  
M1 Polarization ◽  
Chemotactic Protein ◽  
M1 Phenotype

Among the herbal ingredients of HangAmDan-B, a medicinal formula that redirects macrophages to become tumoricidal effectors, we found that Panax notoginseng (Burk.) F. H. Chen is the active component responsible for its macrophage-mediated antitumor activity. The water extracted roots of P. notoginseng (PN) did not affect the viability of RAW264.7 murine macrophage-like cells and murine Lewis lung carcinoma (LLC) cells up to a concentration of 100[Formula: see text][Formula: see text]g/mL. However, the transfer of culture media from PN-treated RAW264.7 cells suppressed the growth of LLC cells. The expression of classically activated (M1) markers, such as interleukin (IL)-1[Formula: see text], monocyte chemotactic protein (MCP)-1, tumor necrosis factor (TNF)-[Formula: see text], and inducible nitric oxide synthase (iNOS), was increased by PN treatment. The expression of alternatively activated (M2) markers including CD206, IL-10, and [Formula: see text]-[Formula: see text]-acetylhexosaminidases (YM-1) was reduced by PN treatment in the presence of IL-4. Flow cytometry also revealed that PN drives M1 activation of RAW264.7 cells. The transfer of culture media from PN-treated RAW264.7 cells induced the apoptosis of LLC cells as measured by flow cytometry using Annexin-V staining and western blot analysis for caspase cascade-related proteins. In addition, the results from in vivo tumor allograft model demonstrated that PN reduced both tumor volume and weight. The activation of macrophages toward an M1 phenotype was confirmed in the tumor allograft tumor model. These results collectively show that PN can serve as a potent anticancer agent through reeducation of macrophages toward an M1 phenotype.

Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage activation via MAPK, PI3k/Akt and NF-κB signaling pathways in RAW264.7 cells

2017 ◽  
Vol 8 (3) ◽  
pp. 1299-1312 ◽  
Author(s):  
Daoyuan Ren ◽  
Dehui Lin ◽  
Aamina Alim ◽  
Quan Zheng ◽  
Xingbin Yang
Keyword(s):  
Signaling Pathways ◽  
Molecular Mechanism ◽  
Macrophage Activation ◽  
Chemical Characterization ◽  
Immunomodulatory Effect ◽  
Raw264.7 Cells ◽  
Raw264.7 Macrophages ◽  
Artemisia Sphaerocephala

The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of the purified Artemisia sphaerocephala Krasch seed polysaccharide (ASKP-1) in RAW264.7 macrophages.

scholarly journals Comparative transcriptome and metabolome analyses provide new insights into the molecular mechanism underlying taproot thickening in Panax notoginseng

2019 ◽  
Author(s):  
Xue-Jiao Li ◽  
Jian-Li Yang ◽  
Ying-Chun Lu ◽  
Zhi-Long Qian ◽  
Ying Li ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Panax Notoginseng ◽  
Chinese Medicinal Herb ◽  
Primary Metabolite ◽  
Developmental Factors ◽  
Complex Biological Process ◽  
Phenylpropanoid Biosynthesis ◽  
Coordinated Expression ◽  
Major Storage Protein

Abstract Background Taproot thickening is a complex biological process and depends on the coordinated expression of the genes controlled by both environmental and developmental factors. Panax notoginseng is an important Chinese medicinal herb characterized by enlarged taproot as the main organ of saponin accumulation. However, little is known about the molecular mechanism of taproot enlargement. Results A total of 29957 DETs were identified during thickening process of P. notoginseng taproot. GO and KEGG pathway enrichment revealed that DETs associated wthith “plant hormone signal transduction”, “starch and sucrose metabolism”, and “phenylpropanoid biosynthesis” were predominantly enriched. Furher functional analysis by integrating DETs expression profiling, endogenous hormone and primary metabolite identified some critical genes (e.g., RNase-like major storage protein, DA1-related protein, Starch branching enzyme I) and primary metabolites (e.g., Sucrose, Glucose, Fructose Malate and Arginine) potentially controlling taproot thickening, and highlighted that hormones crosstalk, transcriptional regulation, homeostasis regulation of sugar and starch, and cell wall metabolism play an important role during thickening process of P. notoginseng taproot. Conclusion These results provide molecular regulatory network of taproot thickening in P. notoginseng and facilitate the characterization of genes responsible for taproot formation in root medicinal plants or crops.

scholarly journals Niacin Ameliorates Neuro-Inflammation in Parkinson’s Disease via GPR109A

2019 ◽  
Vol 20 (18) ◽  
pp. 4559 ◽  
Author(s):  
Banabihari Giri ◽  
Kasey Belanger ◽  
Marissa Seamon ◽  
Eric Bradley ◽  
Sharad Purohit ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Molecular Mechanism ◽  
Inflammatory Cytokines ◽  
Nuclear Translocation ◽  
Raw264.7 Cells ◽  
Anti Inflammatory ◽  
Neuro Inflammation ◽  
Precise Molecular Mechanism ◽  
Pro Inflammatory Cytokines

In this study, we used macrophage RAW264.7 cells to elucidate the molecular mechanism underlying the anti-inflammatory actions of niacin. Anti-inflammatory actions of niacin and a possible role of its receptor GPR109A have been studied previously. However, the precise molecular mechanism of niacin’s action in reducing inflammation through GPR109A is unknown. Here we observed that niacin reduced the translocation of phosphorylated nuclear kappa B (p-NF-κB) induced by lipopolysaccharide (LPS) in the nucleus of RAW264.7 cells. The reduction in the nuclear translocation in turn decreased the expression of pro-inflammatory cytokines IL-1β, IL-6 in RAW264.7 cells. We observed a decrease in the nuclear translocation of p-NF-κB and the expression of inflammatory cytokines after knockdown of GPR109A in RAW264.7 cells. Our results suggest that these molecular actions of niacin are mediated via its receptor GPR109A (also known as HCAR2) by controlling the translocation of p-NF-κB to the nucleus. Overall, our findings suggest that niacin treatment may have potential in reducing inflammation by targeting GPR109A.

Panax notoginseng attenuates LPS-induced pro-inflammatory mediators in RAW264.7 cells

2006 ◽  
Vol 106 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Ava Rhule ◽  
Severine Navarro ◽  
Jerry R. Smith ◽  
David M. Shepherd
Keyword(s):  
Inflammatory Mediators ◽  
Panax Notoginseng ◽  
Raw264.7 Cells

scholarly journals Exploring the molecular mechanism of Panax notoginseng saponins on coronary atherosclerosis let-7b using miRNA

2020 ◽  
Vol 32 (2) ◽  
pp. 1707-1711 ◽  
Author(s):  
Xingli Liu ◽  
Wei Song ◽  
Jingsong Yang ◽  
Zihong Du ◽  
Fangmin Long ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Coronary Atherosclerosis ◽  
Panax Notoginseng ◽  
Panax Notoginseng Saponins

The immunomodulatory effect of docosahexaenoic acid (DHA) on the RAW264.7 cells by modification of the membrane structure and function

2020 ◽  
Vol 11 (3) ◽  
pp. 2603-2616 ◽  
Author(s):  
Nana Bie ◽  
Lirong Han ◽  
Meng Meng ◽  
Zhongli Yan ◽  
Chunling Wang
Keyword(s):  
Docosahexaenoic Acid ◽  
Molecular Mechanism ◽  
Membrane Structure ◽  
Structure And Function ◽  
Immunomodulatory Effect ◽  
Raw264.7 Cells ◽  
Immunomodulatory Activity ◽  
Physiological Functions ◽  
Membrane Structure And Function ◽  
And Function

DHA can regulate various physiological functions of cells. Our group has clarified the immunomodulatory activity and molecular mechanism of DHA on RAW264.7 cells.

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