The effects of methyl jasmonate on growth, gene expression and metabolite accumulation in Isatis indigotica Fort.

2022 ◽  
Vol 177 ◽  
pp. 114482
Author(s):  
Rui Liu ◽  
Zirong Wang ◽  
Jinyu Zheng ◽  
Zhaojin Xu ◽  
Xue Tang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methyl Jasmonate ◽  
Isatis Indigotica ◽  
Metabolite Accumulation

scholarly journals Response of Bioactive Metabolite and Biosynthesis Related Genes to Methyl Jasmonate Elicitation in Codonopsis pilosula

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 533 ◽  
Author(s):  
Jiao-jiao Ji ◽  
Qi Feng ◽  
Hai-feng Sun ◽  
Xue-jun Zhang ◽  
Xiao-xiao Li ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Adventitious Roots ◽  
Multiple Shoots ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Codonopsis Pilosula ◽  
Meja Treatment ◽  
Metabolite Accumulation ◽  
Culture Growth ◽  
Sucrose Level

Bioactive metabolites in Codonopsis pilosula are of particular interest as an immunostimulant. Methyl jasmonate (MeJA) plays an important role in the elicitation of metabolite biosynthesis. Here, we explored the response of metabolites to MeJA elicitation in C. pilosula adventitious roots and multiple shoots. The results showed that the biomass, polysaccharide, and lobetyolin content of adventitious roots exhibited the highest increases with 100 µmol·L−1 MeJA at the 16th day of subculture, whereas the atractylenolide III (a terpenoid) content increased extremely with 50 µmol·L−1 MeJA treatment at the 7th day of subculture. In addition, the biomass and lobetyolin content significantly increased at the 4th day after treatment. Similarly, the polysaccharide and lobetyolin content increased in multiple shoots. Further identification of different metabolites responding to MeJA by 1H-NMR showed an extremely significant increase of the lobetyolinin level, which coincided with lobetyolin. Accordingly, the precursor, fatty acids, showed a highly significant decrease in their levels. Furthermore, a significant increase in β-d-fructose-butanol glycoside was detected, which was accompanied by a decrease in the sucrose level. Accordingly, the enzyme genes responsible for terpenoid and carbohydrate biosynthesis, CpUGPase, and CpPMK, were up regulated. In conclusion, MeJA promoted culture growth and accelerated bioactive metabolite accumulation by regulating the expression of the metabolite biosynthesis related genes, CpUGPase and CpPMK in C. pilosula.

ABA accelerates blackberry (Rubus spp.) fruit ripening by positively affecting ripening-related gene expression and metabolite profiles

2021 ◽  
pp. 1-16
Author(s):  
Chunhong Zhang ◽  
Yaqiong Wu ◽  
Zhenghao Xiong ◽  
Weilin Li ◽  
Wenlong Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Fruit Ripening ◽  
Plant Hormone ◽  
Expression Profiles ◽  
Related Gene ◽  
Commercial Use ◽  
Metabolite Profiles ◽  
Metabolite Accumulation ◽  
Aba Synthesis

BACKGROUND: The softness of blackberry fruits limits their postharvest shelf-life and commercial use, and abscisic acid (ABA) is considered one of the key hormones involved in fruit ripening. OBJECTIVE: This study aimed to explore the underlying physiological and molecular actions of ABA on blackberry fruit ripening and softening. METHODS: Various physiological indices of and plant hormone levels in treated and untreated blackberry fruits were determined simultaneously. The differentially expressed genes (DEGs) were analyzed by RNA-sequencing, and their expression profiles were detected. The ripening mechanism was elucidated by UHPLC-MS using two groups of fruits at 28 d. RESULTS: After 25 d, the ABA concentration and polygalacturonase (PG) and beta-1,4-endoglucanase (EG) activities in ABA-treated fruits were significantly higher than those in untreated fruits. Large differences in the expression profiles were detected at 28 d. The expression of DEGs related to cell wall softening and ABA synthesis was largely triggered after 25 or 28 d. Sixty-nine differentially accumulated metabolites were ultimately annotated as related to fruit ripening. CONCLUSIONS: ABA stimulates blackberry fruit ripening by promoting cell wall enzyme activities, the expression of various ripening-related genes and metabolite accumulation.

Biochemical and gene expression responses of two Iranian grape cultivars to foliar application of methyl jasmonate under boron toxicity conditions

2019 ◽  
Vol 249 ◽  
pp. 355-363 ◽  
Author(s):  
Melina Sarabandi ◽  
Alireza Farokhzad ◽  
Babak Abdollahi Mandoulakani ◽  
Raheleh Ghasemzadeh
Keyword(s):  
Gene Expression ◽  
Methyl Jasmonate ◽  
Foliar Application ◽  
Boron Toxicity ◽  
Grape Cultivars

scholarly journals Temporal and spatial changes in gene expression, metabolite accumulation and phytohormone content in rice seedlings grown under drought stress conditions

2017 ◽  
Vol 90 (1) ◽  
pp. 61-78 ◽  
Author(s):  
Daisuke Todaka ◽  
Yu Zhao ◽  
Takuya Yoshida ◽  
Madoka Kudo ◽  
Satoshi Kidokoro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Stress Conditions ◽  
Rice Seedlings ◽  
Spatial Changes ◽  
Metabolite Accumulation ◽  
Temporal And Spatial
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