Green synthesis of anatase titanium dioxide nanoparticles using Cuminum cyminum seed extract; effect on Mung bean (Vigna radiata) seed germination

2021 ◽  
Vol 126 ◽  
pp. 108485
Author(s):  
Sneha Susan Mathew ◽  
Nisha Elizabeth Sunny ◽  
Venkatkumar Shanmugam
2021 ◽  
pp. 1-18
Author(s):  
Kusum Thakur ◽  
Neerja Khurana ◽  
Neelam Rani ◽  
Vinita Hooda

Abstract Nanosized titanium dioxide (nTiO2) is one of the most diverse nanomaterials available today but inconclusive studies on the effects of nTiO2 on plants are withholding its successful application in agriculture. In the present work, an attempt has been made to evaluate the interaction of nTiO2 with Vigna radiata (L.) Wilczek from the seed germination stage until the plants were 14 days old. TEM analysis revealed that nTiO2 was synthesized in the size range of 1–10 nm and X-ray diffraction (XRD) analysis confirmed the crystal structure. The plants were raised hydroponically in nutrient solution spiked with two different concentrations of nTiO2 (10 and 100 mg L−1). Inductively coupled plasma mass spectrometry (ICP-MS) results established the accumulation of nTiO2 in leaves. In response to the presence of nTiO2, V. radiata plants performed better as indicated by their increased seed germination, root and shoot length, higher fresh and dry weight and elevated chlorophyll and flavonoid contents. Germination percentage of V. radiata seeds increased by about 22% at 10 mg L−1 and 14% at 100 mg L−1 nTiO2 concentration. Maximum stimulation of total chlorophyll, flavonoids and phenolic contents was observed at 100 mg L−1 nTiO2 concentration in 7-day old plants, where an astonishing 8 fold increase in chlorophyll, 3 fold increase in flavonoids and 2 fold increase in phenolics was observed. However, a decrease in carbohydrate and protein contents and an increase in lipid peroxidation also marked the presence of mild oxidative stress that was neutralized by increased activity of antioxdant enzymes namely catalase, glutathione reductase and glutathione-s-transferase. The activity of superoxide dismutase was more or less stable while glutathione peroxidase activity reduced compared to the control plants. The higher ABTS and DPPH free radical scavenging activities of the nTiO2 treated plants also supported effective neutralization of free radicals.


2014 ◽  
Vol 7 (12) ◽  
pp. 968-976 ◽  
Author(s):  
Thirunavukkarasu Santhoshkumar ◽  
Abdul Abdul Rahuman ◽  
Chidambaram Jayaseelan ◽  
Govindasamy Rajakumar ◽  
Sampath Marimuthu ◽  
...  

2022 ◽  
Vol 34 (2) ◽  
pp. 409-414
Author(s):  
N. Usha Rani ◽  
P. Pavani ◽  
P.T.S.R.K. Prasad Rao

Titanium nanoparticles are toxic to bacteria and have a widespread applications in different fields of research. Hence the present study aimed to synthesize the titanium dioxide nanoparticles by adopting green synthesis methodology using Kigelia africana leave extract as a biological reducing agent. The UV absorption spectra show characteristic absorption maxima corresponding to TiO2 nanoparticles at a wavelength of 512 nm confirms the formation of nanosized tin particles. The FT-IR spectrum of TiO2 nanoparticles show absorption bands at 3609 cm-1 and 3227 cm-1 corresponding to O-H stretching in alcoholic and carboxylic compounds, respectively. Absorption peaks at 1607, 2834, 1654 and 1324 cm-1 correspond to aromatic C=C vibrations, C-H stretching in aldehydes, C-H bending vibrations and aromatic C-N stretching vibrations, respectively. This confirms the involvement of bioactive compounds from the plant extract. The SEM and EDX studies confirmed that the nanoparticles are spherical to oval shape with an average particle size of 46 nm. The metal content in the nanoparticles was found to be 58.71%. The synthesized nanoparticles have potential growth inhibition activity against Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). The DPPH radical scavenging activity of the nanoparticles synthesized was compared with that of aqueous leaf extract and standard ascorbic acid and proved that the nanoparticles have enhanced activity than aqueous leaf extract. The IC50 of the leaf extract, nanoparticles and the standard was found to be 31.55, 75.82 and 84.95 μg/mL, respectively. Kigelia africana leaf is shown in this work to be a valuable bioagent in the biosynthesis of TiO2 nanoparticles with increased biological activity.


2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
S. Rajeshkumar ◽  
J. Santhoshkumar ◽  
Leta Tesfaye Jule ◽  
Krishnaraj Ramaswamy

Phytosynthesis particles are the efficient activity of biomedical and environmental. In this present study, the green synthesis of titanium dioxide (TiO2) nanoparticles using the king of bitter herbal plant Andrographis paniculata was synthesized and characterized using XRD, SEM, HRTEM, AFM, and antimicrobial, antioxidant, and antidiabetic activities. The size of the particles HRTEM shows 50 nm, and SEM shows the spherical shape, which reveals the synthesis of TiO2 nanoparticles. XRD spectrum shows crystallinity of nanoparticles, and an average size is calculated about 22.97 nm. The phytosynthesis TiO2 shows the antioxidant and antidiabetic activities. Similarly, toxicity studies have demonstrated the hatching and viability LD 50 value of TiO2 250 μg/L. The current study’s findings suggested that phytosynthesis TiO2 using extract of Andrographis paniculata exposure to potential hazard factors to biomedical and environmental uses.


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