Enhanced growth and antioxidant efficiency of Vigna radiata seedlings in the presence of titanium dioxide nanoparticles synthesized via the sonochemical method

Abstract Nanosized titanium dioxide (nTiO2) is one of the most diverse nanomaterials available today but inconclusive studies on the effects of nTiO2 on plants are withholding its successful application in agriculture. In the present work, an attempt has been made to evaluate the interaction of nTiO2 with Vigna radiata (L.) Wilczek from the seed germination stage until the plants were 14 days old. TEM analysis revealed that nTiO2 was synthesized in the size range of 1–10 nm and X-ray diffraction (XRD) analysis confirmed the crystal structure. The plants were raised hydroponically in nutrient solution spiked with two different concentrations of nTiO2 (10 and 100 mg L−1). Inductively coupled plasma mass spectrometry (ICP-MS) results established the accumulation of nTiO2 in leaves. In response to the presence of nTiO2, V. radiata plants performed better as indicated by their increased seed germination, root and shoot length, higher fresh and dry weight and elevated chlorophyll and flavonoid contents. Germination percentage of V. radiata seeds increased by about 22% at 10 mg L−1 and 14% at 100 mg L−1 nTiO2 concentration. Maximum stimulation of total chlorophyll, flavonoids and phenolic contents was observed at 100 mg L−1 nTiO2 concentration in 7-day old plants, where an astonishing 8 fold increase in chlorophyll, 3 fold increase in flavonoids and 2 fold increase in phenolics was observed. However, a decrease in carbohydrate and protein contents and an increase in lipid peroxidation also marked the presence of mild oxidative stress that was neutralized by increased activity of antioxdant enzymes namely catalase, glutathione reductase and glutathione-s-transferase. The activity of superoxide dismutase was more or less stable while glutathione peroxidase activity reduced compared to the control plants. The higher ABTS and DPPH free radical scavenging activities of the nTiO2 treated plants also supported effective neutralization of free radicals.

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Green synthesis of anatase titanium dioxide nanoparticles using Cuminum cyminum seed extract; effect on Mung bean (Vigna radiata) seed germination

Inorganic Chemistry Communications ◽

10.1016/j.inoche.2021.108485 ◽

2021 ◽

Vol 126 ◽

pp. 108485

Author(s):

Sneha Susan Mathew ◽

Nisha Elizabeth Sunny ◽

Venkatkumar Shanmugam

Keyword(s):

Titanium Dioxide ◽

Seed Germination ◽

Green Synthesis ◽

Vigna Radiata ◽

Mung Bean ◽

Titanium Dioxide Nanoparticles ◽

Seed Extract ◽

Cuminum Cyminum

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Biosynthesis and Characterization of Titanium Dioxide Nanoparticles Using Pithecellobium Dulce and Lagenaria Siceraria Aqueous Leaf Extract and Screening their Free Radical Scavenging and Antibacterial Properties

International Letters of Chemistry Physics and Astronomy ◽

10.18052/www.scipress.com/ilcpa.50.80 ◽

2015 ◽

Vol 50 ◽

pp. 80-95 ◽

Cited By ~ 7

Author(s):

S. Kalyanasundaram ◽

M. Jeevan Prakash

Keyword(s):

Titanium Dioxide ◽

New Technologies ◽

Titanium Dioxide Nanoparticles ◽

Radical Scavenging ◽

Antibacterial Properties ◽

Biological Method ◽

Lagenaria Siceraria ◽

Biological Behaviour ◽

Synthesis Of Nanoparticles ◽

Xrd Techniques

The recent development and implementation of new technologies have led to new era, the nanorevolution which unfolds role of plants in bio and green synthesis of nanoparticles which seem to have drawn quite an unequivocal attention with a view of synthesizing stable nanoparticles. In this present work, we have compared titanium dioxide nanocrystals synthesized by both chemical method and biological method from Titanium tetra isopropoxide as precursor. The biological method was performed by using the aqueous plant extract of Pithecellobium dulce and Lagenaria siceraria. The synthesized nanocrystals were characterized by FTIR Spectroscopy, Scanning Electron Microscopy (SEM), Energy Dispersive Xray (EDAX), Transmission Electron Microscope (TEM) and X-ray diffraction (XRD) techniques and also Antioxidant assay and antimicrobial test were performed to evaluate its biological behaviour. The nanocrystals synthesized by biological method were found to have higher antioxidant potential, antimicrobial activity than chemically synthesized.

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Facile Green Synthesis and Characterization of Titanium Dioxide Nanoparticles Using Kigelia africana (Lam) Benth., Aqueous Leaf Extract and its Antioxidant and Antibacterial Activity

Asian Journal of Chemistry ◽

10.14233/ajchem.2022.23563 ◽

2022 ◽

Vol 34 (2) ◽

pp. 409-414

Author(s):

N. Usha Rani ◽

P. Pavani ◽

P.T.S.R.K. Prasad Rao

Keyword(s):

Titanium Dioxide ◽

Green Synthesis ◽

Tio2 Nanoparticles ◽

Leaf Extract ◽

Titanium Dioxide Nanoparticles ◽

Average Particle Size ◽

Radical Scavenging ◽

Average Particle ◽

Absorption Bands ◽

Aqueous Leaf Extract

Titanium nanoparticles are toxic to bacteria and have a widespread applications in different fields of research. Hence the present study aimed to synthesize the titanium dioxide nanoparticles by adopting green synthesis methodology using Kigelia africana leave extract as a biological reducing agent. The UV absorption spectra show characteristic absorption maxima corresponding to TiO2 nanoparticles at a wavelength of 512 nm confirms the formation of nanosized tin particles. The FT-IR spectrum of TiO2 nanoparticles show absorption bands at 3609 cm-1 and 3227 cm-1 corresponding to O-H stretching in alcoholic and carboxylic compounds, respectively. Absorption peaks at 1607, 2834, 1654 and 1324 cm-1 correspond to aromatic C=C vibrations, C-H stretching in aldehydes, C-H bending vibrations and aromatic C-N stretching vibrations, respectively. This confirms the involvement of bioactive compounds from the plant extract. The SEM and EDX studies confirmed that the nanoparticles are spherical to oval shape with an average particle size of 46 nm. The metal content in the nanoparticles was found to be 58.71%. The synthesized nanoparticles have potential growth inhibition activity against Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). The DPPH radical scavenging activity of the nanoparticles synthesized was compared with that of aqueous leaf extract and standard ascorbic acid and proved that the nanoparticles have enhanced activity than aqueous leaf extract. The IC50 of the leaf extract, nanoparticles and the standard was found to be 31.55, 75.82 and 84.95 μg/mL, respectively. Kigelia africana leaf is shown in this work to be a valuable bioagent in the biosynthesis of TiO2 nanoparticles with increased biological activity.

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Fabrication of Titanium Dioxide Nanoparticles Using Sunflower Leaf Extract and Their Applications Towards the Synthesis and Biological Evaluation of Some Novel Phenanthro Imidazole Derivatives

Biointerface Research in Applied Chemistry ◽

10.33263/briac123.33723389 ◽

2021 ◽

Vol 12 (3) ◽

pp. 3372-3389

Keyword(s):

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles ◽

Radical Scavenging ◽

Biological Evaluation ◽

Solution Stability ◽

Simple Method ◽

Tio2 Nps ◽

X Ray ◽

Imidazole Derivatives ◽

Vis Spectroscopy

Plant-mediated synthesis of nanomaterials has been increasingly practiced day by day due to its eco-friendly nature, simple method without using any hazardous chemicals and solvent. In the present study, we synthesized Titanium dioxide nanomaterials (TiO2 NPs) using sunflower leaves extract. The synthesized TiO2 NPs was confirmed by ultraviolet-visible (UV-Vis) spectroscopy, while morphology and size of the NPs were identified by Scanning Electron Microscopy (SEM); elemental composition of materials analyzed by using Energy Dispersive X-ray (EDAX) analysis, and functional groups in plant extract identified by using Fourier Transform Infrared Spectroscopy (FTIR) analysis responsible for reducing and stabilizing of NPs, crystalline nature of NPs identification by using X-ray diffraction analysis (XRD), colloidal solution stability, the thermal stability of NPs noticed by dynamic light scattering (DLS) and thermo-gravimetric analyses. The synthesized green catalyst TiO2 NPs mediated novel Phenenthro [9,10-d imidazole derivatives(9a-e) have been successfully synthesized with good yields by reacting to 3-methyl-5-phenoxy-1-phenyl-1H-pyrazole-4-carbaldehydes with 9,10-phenanthrene quinone and ammonium acetate. The structures of the synthesized molecules were characterized by NMRspectroscopy. The fluorescence property of the synthesized molecules (9a-e) in acetonitrile solvent was studied, and all the compounds showed good emission intensity. An in-silico molecular docking was performed on the synthesized molecules (9a-e) using aldose reductase as a target protein. Overall, studies indicate that compounds (9a-e)are promising in developing novel anti-diabetic drugs in the future. The synthesized molecules (9a-e) were further screened for antioxidant activity by DPPH radical scavenging method.

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Effect of Titanium Dioxide Nanoparticles and Carbon Nanotube on Seed Germination Characteristics of Some Hordeum vulgare Hulled Varieties

Iranian Journal of Seed Research ◽

10.29252/yujs.3.2.119 ◽

2017 ◽

Vol 3 (2) ◽

pp. 119-127

Author(s):

Mahmod Reza Tadayon ◽

Mohammad Rahimi

Keyword(s):

Titanium Dioxide ◽

Carbon Nanotube ◽

Hordeum Vulgare ◽

Seed Germination ◽

Titanium Dioxide Nanoparticles ◽

Germination Characteristics

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Titanium dioxide nanoparticles alleviate cadmium toxicity in lentil (Lens culinaris Medic) seeds

Acta agriculturae Slovenica ◽

10.14720/aas.2020.116.1.1116 ◽

2020 ◽

Vol 116 (1) ◽

pp. 59

Author(s):

Hassan FEIZI ◽

Nafiseh AGHELI ◽

Hossein SAHABI

Keyword(s):

Titanium Dioxide ◽

Seed Germination ◽

Titanium Dioxide Nanoparticles ◽

Germination Rate ◽

Cadmium Toxicity ◽

Cadmium Concentration ◽

Life Cycles ◽

Control Group ◽

Germination Time ◽

Mean Germination Time

This research was carried out with the aim of investigating the effects of different concentrations of titanium dioxide nanoparticles and cadmium on lentil seed germination and seedling early growth. The experiment was conducted as a factorial layout based on a completely randomized design with four replicates. Test plants were exposed to 0, 100, 250, and 500 ppm of cadmium, and then amended with 0, 100, 200, and 300 ppm of TiO2 nanoparticles. Results demonstrated that the application of 300 ppm of titanium dioxide nanoparticles improved the mean germination time (MGT) and seed germination rate by 39 % and 62 % respectively. Most traits exhibited a decreasing trend as the concentration of cadmium increased. Furthermore, application of 500 ppm of cadmium concentration increased mean germination time compared to the control group (1.667 day). In conclusion, the application of titanium dioxide nanoparticles averted the effect of high cadmium stress on lentils, and maintained the length of the shoot compared to the control. However, further studies on plant life cycles should be performed to detect the inhibitory effects of titanium dioxide on heavy metal toxicity.

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Effects of the Reactive Moiety of Phenolipids on Their Antioxidant Efficiency in Model Emulsified Systems

Foods ◽

10.3390/foods10051028 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1028

Author(s):

Marlene Costa ◽

Sonia Losada-Barreiro ◽

Júlia Magalhães ◽

Luís S. Monteiro ◽

Carlos Bravo-Díaz ◽

...

Keyword(s):

Olive Oil ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Fold Increase ◽

Interfacial Region ◽

Antioxidant Efficiency ◽

Alkyl Chains ◽

Oil In Water ◽

Oxidative Protection ◽

Dpph Radical Scavenging

Our previous research was focused on the effects of hydrophobicity on the antioxidant (AO) efficiency of series of homologous antioxidants with the same reactive moieties. In this work we evaluate the antioxidant efficiency of hydrophobic phenolipids in 4:6 olive oil-in-water emulsions, with different phenolic moieties (derived from caffeic, 4-hydroxycinnamic, dihydrocaffeic acids, tyrosol and hydroxytyrosol), with alkyl chains of 8 and 16 carbons, and compare the antioxidant efficiency with that of the parent compounds. All catecholic phenolipids, in particular the C8 derivatives, have proven to be better antioxidants for the oxidative protection of emulsions than their parental compounds with octyl dihydrocafffeate being the most efficient (16-fold increase in relation to the control). To understand the importance of some factors on the antioxidant efficiency of compounds in emulsions, Pearson’s correlation analysis was carried out between antioxidant activity and the first anodic potential (Epa), reducing capacity (FRAP value), DPPH radical scavenging activity (EC50) and the concentration of antioxidants in each region of the emulsified system. Results confirm the importance of the effective concentration of AOs in the interfacial region (AOI) (ρ = 0.820) and of the Epa (ρ = −0.677) in predicting their antioxidant efficiency in olive oil-in-water emulsions.

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Titanium Dioxide Nanoparticles and Sodium Nitroprusside Alleviate the Adverse Effects of Cadmium Stress on Germination and Seedling Growth of Wheat (Triticum aestivum L.)

Universitas Scientiarum ◽

10.11144/javeriana.sc23-1.tdna ◽

2018 ◽

Vol 23 (1) ◽

pp. 61 ◽

Cited By ~ 9

Author(s):

Javad Faraji ◽

Ali Sepehri

Keyword(s):

Titanium Dioxide ◽

Seed Germination ◽

Adverse Effects ◽

Sodium Nitroprusside ◽

Seedling Growth ◽

Titanium Dioxide Nanoparticles ◽

Wheat Seed ◽

Cd Stress ◽

Tio2 Nps ◽

Germination Indices

Effect of titanium dioxide nanoparticles (TiO2 NPs) (0, 500, 1 000 and 2 000 mg/L) and sodium nitroprusside (SNP) (0 and 100 M) as nitric oxide (NO) donor, on wheat seed germination and seedling growth were investigated under cadmium (Cd) stress (0, 50 and 100 mM CdCl2). Concentration-dependent declining trends were observed in wheat germination indices upon seed exposure to CdCl2 suspensions which were more obvious under higher Cd stress. Exogenous sodium nitroprusside (SNP) and TiO2 nanoparticles (NPs) positively affected most germination indices under normal and stress conditions. In most cases, combined application of TiO2 NPs and SNP suspensions boosted stimulatory function of both compounds and moderated adverse effects of Cd treatments on wheat seed germination and seedling growth. 2 000 mg/L TiO2 + SNP (100 M) treatment recorded the best results regarding most germination indices under lower and higher (50 and 100 mM CdCl2) Cd stress. Overall, it could be concluded that application of TiO2 NPs in combination with SNP might be a promising approach in counteracting the adverse effects of Cd stress on wheat seed germination and early growth.

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