Effect of Traditional Chinese Medicine (TCM) in moderate-to-severe eczema in clinic and animal model: beyond corticosteroids

This research investigated the anti-hypertension effect of the traditional Chinese medicine (TCM) Ju-Ling-Tang (JLT) on an animal model of hypertension induced by unilateral renal artery ligation. In the study of anti-hypertension effects, 60 minutes after oral administration with NG tube feeding of 240 mg/kg JLT, a significant decrease in blood pressure ( p < 0.05) was observed and sustained till 120 minutes. In the group given 50 mg/kg α-methyldopa orally, the effect was obvious 90 minutes after medication ( p < 0.01), and lasted until 240 minutes. In terms of organ pathology, a significant reduction in the extent of induced glomerular sclerosis was observed in rats given 240 mg/kg JLT compared with the control. From these results, we infer that JLT has a beneficial anti-hypertensive effect on renal hypertension.

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Model Organisms and Traditional Chinese Medicine Syndrome Models

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/761987 ◽

2013 ◽

Vol 2013 ◽

pp. 1-14 ◽

Cited By ~ 7

Author(s):

Shuang Ling ◽

Jin-Wen Xu

Keyword(s):

Animal Model ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Evaluation System ◽

Pharmacological Action ◽

Modern Medicine ◽

Model Organisms ◽

Traditional Chinese Medicine Syndrome ◽

The Difference ◽

Tcm Syndrome

Traditional Chinese medicine (TCM) is an ancient medical system with a unique cultural background. Nowadays, more and more Western countries due to its therapeutic efficacy are accepting it. However, safety and clear pharmacological action mechanisms of TCM are still uncertain. Due to the potential application of TCM in healthcare, it is necessary to construct a scientific evaluation system with TCM characteristics and benchmark the difference from the standard of Western medicine. Model organisms have played an important role in the understanding of basic biological processes. It is easier to be studied in certain research aspects and to obtain the information of other species. Despite the controversy over suitable syndrome animal model under TCM theoretical guide, it is unquestionable that many model organisms should be used in the studies of TCM modernization, which will bring modern scientific standards into mysterious ancient Chinese medicine. In this review, we aim to summarize the utilization of model organisms in the construction of TCM syndrome model and highlight the relevance of modern medicine with TCM syndrome animal model. It will serve as the foundation for further research of model organisms and for its application in TCM syndrome model.

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Animal Model of Aplastic Anemia Based on the Characteristics of Clinical Diagnosis and Treatment of Traditional Chinese Medicine and Modern Medicine

DEStech Transactions on Materials Science and Engineering ◽

10.12783/dtmse/mmme2016/10142 ◽

2017 ◽

Author(s):

Xu ZHANG ◽

Ming-san Miao

Keyword(s):

Animal Model ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Aplastic Anemia ◽

Clinical Diagnosis ◽

Modern Medicine ◽

Diagnosis And Treatment

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BUILDING AND EVALUATING AN ANIMAL MODEL FOR SYNDROME IN TRADITIONAL CHINESE MEDICINE IN THE CONTEXT OF UNSTABLE ANGINA (MYOCARDIAL ISCHEMIA) BY SUPERVISED DATA MINING APPROACHES

Journal of Biological System ◽

10.1142/s0218339009003022 ◽

2009 ◽

Vol 17 (04) ◽

pp. 531-546 ◽

Cited By ~ 13

Author(s):

SHUZHEN GUO ◽

JIANXIN CHEN ◽

HUIHUI ZHAO ◽

WEI WANG ◽

JIANQIANG YI ◽

...

Keyword(s):

Data Mining ◽

Animal Model ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Myocardial Ischemia ◽

Unstable Angina ◽

Clinical Epidemiology ◽

Blood Stasis ◽

Blood Stasis Syndrome ◽

Novel Strategy

Building an animal model for a disease is a better avenue to understand the inner mechanism of it. Traditional Chinese Medicine accumulated much practical experience and a large amount of literature to heal diseases during the past 3000 years. However, as there is no available animal model for TCM research because syndrome, the core of TCM theory, it is hard to be diagnosed from animals. In this paper, we present a novel strategy to build and evaluate an animal model for syndrome in TCM in the context of a disease. We first carried out a clinical epidemiology survey for a syndrome (Blood stasis syndrome, BSS) diagnosed by TCM experts in the context of a disease (Unstable angina, UA). Meanwhile, the blood samples of patients included in the survey were collected and measured as physical and chemical specifications by laboratory examinations. Alternatively, we used supervised data mining methods to build association between the specifications and the syndrome in the context of UA. The accuracy of classification was used to evaluate performance of the association built. Finally, we built an animal model for myocardial ischemia and validated the model by established diagnosis criterion of myocardial ischemia. Furthermore, the built association was used to evaluate whether an animal is with BSS. The results indicated that the strategy successfully evaluates and separates the animal model for syndrome in TCM from the counterpart for myocardial ischemia. The novel strategy presented in the paper provides a better insight to understand the nature of syndrome in TCM and pave a basis for personalized therapies of UA.

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Exploring anti-nonalcoholic fatty liver disease mechanism of Gardeniae Fructus by combining an animal model with network pharmacology and molecular docking

10.21203/rs.3.rs-23225/v1 ◽

2020 ◽

Author(s):

Lin Li ◽

Lihua Lu ◽

Zhong-yan Tang ◽

Zhengxiang Xia

Keyword(s):

Animal Model ◽

Molecular Docking ◽

Surface Plasmon Resonance ◽

Liver Disease ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Fatty Liver Disease ◽

Target Protein ◽

Network Pharmacology ◽

Nonalcoholic Fatty Liver

Abstract Background Gardeniae Fructus (GF), a traditional Chinese medicine in clinic for the treatment of nonalcoholic fatty liver disease (NAFLD). However, the mechanisms of action of GF was still margin. To explore the efficacy and mechanism of action of GF for the treatment of NAFLD, we proposed a strategy combined in vivo efficacy verification, network pharmacology analysis, molecular docking, and validity assay of target protein. Methods Firstly, an animal model induced by the high fat diet feed was established, then orally administrated with GF, the mRNA expression levels of lipogenesis was performed by RT-PCR, the liver tissue specimens were stained by hematoxylin and eosin (H&E), then observed by light microscopy. Secondly, network pharmacology studies clarified the relationship among the active constituents, target protein, and pathways, and then explored by the molecular docking. Finally, validity assay of target protein was performed in surface plasmon resonance (SPR) test. Results GF protected against NAFLD in rats. Network pharmacology showed that quercetin, oleanolic acid, and geniposide, targeted on PPARα, PPARγ, and CA2 genes, through regulating PPAR, AMPK, and cGMP-PKG signal pathways, to protect against NAFLD. And the Conclusion GF could alleviate NAFLD through the molecular mechanisms explored by network pharmacology, molecular docking, and surface plasmon resonance, those method can be effective tools to clarify the mechanisms of actions of traditional Chinese medicine from a holistic perspective.

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Establishment and applicability evaluation of animal model which was suitable to evaluate immediate hypersensitivity induced by injections of traditional Chinese medicine in BN rats

China Journal of Chinese Materia Medica ◽

10.4268/cjcmm20111402 ◽

2011 ◽

Author(s):

GUO Shanshan

Keyword(s):

Animal Model ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Immediate Hypersensitivity ◽

Applicability Evaluation

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Application of morbid animal model in drug safety evaluation of traditional Chinese medicine

Frontiers in Pharmacology ◽

10.3389/fphar.2015.00037 ◽

2015 ◽

Vol 6 ◽

Cited By ~ 3

Author(s):

Ning Ke-Yong ◽

Li Min

Keyword(s):

Animal Model ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Drug Safety ◽

Safety Evaluation ◽

Drug Safety Evaluation

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Development of the novel antiplatelet agent ADTM, originating from traditional Chinese medicine: a chemical proteomic analysis and in-vivo assessment of efficacy in an animal model

The Lancet ◽

10.1016/s0140-6736(16)31964-x ◽

2016 ◽

Vol 388 ◽

pp. S37 ◽

Cited By ~ 4

Author(s):

Guozhen Cui ◽

Angela Wa Ho ◽

Simon Ming Yuen Lee ◽

Yuqiang Wang ◽

Maggie Pui Man Hoi

Keyword(s):

Animal Model ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Proteomic Analysis ◽

Antiplatelet Agent ◽

The Novel ◽

In Vivo Assessment

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Improvement of skin lesions in corticosteroid withdrawal-associated severe eczema by multicomponent traditional Chinese medicine therapy

Allergy Asthma & Clinical Immunology ◽

10.1186/s13223-021-00555-0 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Serife Uzun ◽

Zixi Wang ◽

Tory A. McKnight ◽

Paul Ehrlich ◽

Erin Thanik ◽

...

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Withdrawal Syndrome ◽

Medical Condition ◽

Skin Lesions ◽

High Dose ◽

Topical Steroids ◽

Rapid Improvement ◽

Corticosteroid Withdrawal ◽

Severe Eczema

Abstract Rationale We recently showed that multicomponent traditional Chinese medicine (TCM) therapy had steroid-sparing effects in moderate-to-severe eczema. We sought to evaluate TCM effects in severe eczema in a 7-year-old male with refractory disease and corticosteroid withdrawal syndrome. Methods Prior to referral, the patient had been treated since infancy with increasingly intensive standard of care, including high-dose topical and systemic corticosteroid and antibiotic therapy and was unable to tolerate further steroid treatment. The patient was administered a combination of oral and topical TCM for 17 months following discontinuation of his steroid regimen. His overall medical condition was assessed by SCORAD criteria and laboratory evaluations of serum IgE, absolute eosinophil count, and liver and kidney function tests. Results The patient showed rapid improvement of clinical measures of disease after starting TCM therapy, with marked improvement of sleep quality within the first week, complete resolution of itching, oozing, and erythema at 2 weeks, and a 79% and 99% decrease in his SCORAD values after one month and 3–6 months of TCM, respectively. Serum total IgE decreased by 75% (from 19,000 to 4630 (kIU/L), and absolute eosinophil counts decreased by 60% (from 1000 to 427 cells/μL) after 12 months of treatment. The patient did not require oral or topical steroids during the 17-month trial of TCM. TCM was tapered without complications. His dermatologic manifestations continued to be well-controlled 3 months after discontinuation. Conclusion This case study suggests TCM should be further evaluated in controlled clinical studies of patients with severe, refractory eczema and steroid withdrawal syndrome.

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