Structured light scanning for high-resolution documentation of in situ archaeological finds

2009 ◽  
Vol 36 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Shannon P. McPherron ◽  
Tim Gernat ◽  
Jean-Jacques Hublin
Keyword(s):  
High Resolution ◽  
Structured Light ◽  
Structured Light Scanning

scholarly journals 3D INSPECTION OF THE RESTORATION AND CONSERVATION OF STAINED GLASS WINDOWS USING HIGH RESOLUTION STRUCTURED LIGHT SCANNING

2019 ◽  
Vol XLII-2/W15 ◽  
pp. 965-972
Author(s):  
M. Rahrig ◽  
M. Torge
Keyword(s):  
High Resolution ◽  
Structured Light ◽  
High Accuracy ◽  
Surface Measurement ◽  
Stained Glass ◽  
Structured Light Scanning ◽  
Before And After ◽  
Time Periods ◽  
Restoration Measures ◽  
Initial Focus

<p><strong>Abstract.</strong> The initial focus of this research was on the development of a general workflow for the documentation and monitoring of historical stained glass windows using structured light scanning. Therefore windows from different churches, time periods and with different corrosion and damage phenomena were scanned before and after conservation measures. To evaluate the execution of the restoration measures the data was compared using 3D inspection software to examine the differences in geometry between the two scans. Various problems had to be solved, for example, how to deal with heavily reflective surfaces and the extreme contrast between light and dark surfaces, as seen in the borders between ‘Schwarzlot’ painting and plain glass. The application of materials for matting the surfaces, such as Cyclododecane spray, was impossible due to the high accuracy of the surface measurement required for 3D inspection. Regarding the contrast differences of the surfaces, the creation of exposure fusions and the use of polarization filters to reduce reflections were tested. In addition to the general problems encountered when recording translucent surfaces, the historical glasses caused additional problems in calculating surface comparisons. For example, the windows have to be moved and turned around several times, both during the conservation process and while scanning, causing deformations of the geometry due to the flexible lead rods allowing a certain degree of movement.</p>

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

High-Resolution Resistance Mapping in SEM

2018 ◽  
Author(s):  
Grigore Moldovan ◽  
Wolfgang Joachimi ◽  
Guillaume Boetsch ◽  
Jörg Jatzkowski ◽  
Frank Altman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Reference Structure ◽  
Total Resistance ◽  
Embedded Electronics ◽  
Improved Performance ◽  
Mapping Techniques ◽  
Scanning Electron

Abstract This work presents advanced resistance mapping techniques based on Scanning Electron Microscopy (SEM) with nanoprobing systems and the related embedded electronics. Focus is placed on recent advances to reduce noise and increase speed, such as integration of dedicated in situ electronics into the nanoprobing platform, as well as an important transition from current-sensitive to voltagesensitive amplification. We show that it is now possible to record resistance maps with a resistance sensitivity in the 10W range, even when the total resistance of the mapped structures is in the range of 100W. A reference structure is used to illustrate the improved performance, and a lowresistance failure case is presented as an example of analysis made possible by these developments.

scholarly journals A High-Precision Registration Technology Based on Bundle Adjustment in Structured Light Scanning System

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Jianying Yuan ◽  
Qiong Wang ◽  
Xiaoliang Jiang ◽  
Bailin Li
Keyword(s):  
High Precision ◽  
Large Scale ◽  
Structured Light ◽  
Bundle Adjustment ◽  
Scanning System ◽  
Control Points ◽  
Registration Method ◽  
Global Control ◽  
3D Data ◽  
Structured Light Scanning

The multiview 3D data registration precision will decrease with the increasing number of registrations when measuring a large scale object using structured light scanning. In this paper, we propose a high-precision registration method based on multiple view geometry theory in order to solve this problem. First, a multiview network is constructed during the scanning process. The bundle adjustment method from digital close range photogrammetry is used to optimize the multiview network to obtain high-precision global control points. After that, the 3D data under each local coordinate of each scan are registered with the global control points. The method overcomes the error accumulation in the traditional registration process and reduces the time consumption of the following 3D data global optimization. The multiview 3D scan registration precision and efficiency are increased. Experiments verify the effectiveness of the proposed algorithm.

scholarly journals Melite Civitas Romana in 3D: Virtualization Project of the Archaeological Park and Museum of the Domus Romana of Rabat, Malta

2021 ◽  
Vol 7 (1) ◽  
pp. 51-83
Author(s):  
Davide Tanasi ◽  
Stephan Hassam ◽  
Kaitlyn Kingsland ◽  
Paolo Trapani ◽  
Matthew King ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Structured Light ◽  
Terrestrial Laser Scanning ◽  
Archaeological Site ◽  
South Florida ◽  
Virtual Museum ◽  
Current State ◽  
Structured Light Scanning ◽  
The University ◽  
Archaeological Excavations

Abstract The archaeological site of the Domus Romana in Rabat, Malta was excavated almost 100 years ago yielding artefacts from the various phases of the site. The Melite Civitas Romana project was designed to investigate the domus, which may have been the home of a Roman Senator, and its many phases of use. Pending planned archaeological excavations designed to investigate the various phases of the site, a team from the Institute for Digital Exploration from the University of South Florida carried out a digitization campaign in the summer of 2019 using terrestrial laser scanning and aerial digital photogrammetry to document the current state of the site to provide a baseline of documentation and plan the coming excavations. In parallel, structured light scanning and photogrammetry were used to digitize 128 artefacts in the museum of the Domus Romana to aid in off-site research and create a virtual museum platform for global dissemination.

scholarly journals Venus: key to understanding the evolution of terrestrial planets

2021 ◽  
Author(s):  
Colin F. Wilson ◽  
Thomas Widemann ◽  
Richard Ghail
Keyword(s):  
High Temperature ◽  
High Resolution ◽  
Radar Imaging ◽  
Space Science ◽  
Interior Surface ◽  
Extended Surface ◽  
Earth Like Planets ◽  
Orbital Mission

AbstractIn this paper, originally submitted in answer to ESA’s “Voyage 2050” call to shape the agency’s space science missions in the 2035–2050 timeframe, we emphasize the importance of a Venus exploration programme for the wider goal of understanding the diversity and evolution of habitable planets. Comparing the interior, surface, and atmosphere evolution of Earth, Mars, and Venus is essential to understanding what processes determined habitability of our own planet and Earth-like planets everywhere. This is particularly true in an era where we expect thousands, and then millions, of terrestrial exoplanets to be discovered. Earth and Mars have already dedicated exploration programmes, but our understanding of Venus, particularly of its geology and its history, lags behind. Multiple exploration vehicles will be needed to characterize Venus’ richly varied interior, surface, atmosphere and magnetosphere environments. Between now and 2050 we recommend that ESA launch at least two M-class missions to Venus (in order of priority): a geophysics-focussed orbiter (the currently proposed M5 EnVision orbiter – [1] – or equivalent); and an in situ atmospheric mission (such as the M3 EVE balloon mission – [2]). An in situ and orbital mission could be combined in a single L-class mission, as was argued in responses to the call for L2/L3 themes [3–5]. After these two missions, further priorities include a surface lander demonstrating the high-temperature technologies needed for extended surface missions; and/or a further orbiter with follow-up high-resolution surface radar imaging, and atmospheric and/or ionospheric investigations.

Sign in / Sign up

Export Citation Format

Share Document