scholarly journals BAd-CRISPR: inducible gene knockout in interscapular brown adipose tissue of adult mice

2021 ◽  
pp. 101402
Author(s):  
Steven M. Romanelli ◽  
Kenneth T. Lewis ◽  
Akira Nishii ◽  
Alan C. Rupp ◽  
Ziru Li ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Gene Knockout ◽  
Interscapular Brown Adipose Tissue ◽  
Adult Mice ◽  
Brown Adipose ◽  
Inducible Gene

Evidence for the Mediatory Role of Brown Adipose Tissue During Nonshivering Thermogenesis in the Cold-Acclimated Mouse

1972 ◽  
Vol 50 (2) ◽  
pp. 168-170 ◽  
Author(s):  
J. S. Hayward ◽  
P. F. Davies
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
The Body ◽  
Brown Fat ◽  
Interscapular Brown Adipose Tissue ◽  
Adult Mice ◽  
Brown Adipose ◽  
Rate Of Oxygen Consumption

The increased rate of oxygen consumption by cold-acclimated, adult mice after subcutaneous injection of noradrenaline has been measured for intact individuals and for those with the arterial supply to their interscapular brown adipose tissue ligated. An immediate reduction of 40% of this calorigenic response was noted in mice thus operated. Dissection of the total brown fat of the body indicated that the interscapular deposits comprise 43% by weight of the total brown adipose tissue, which in turn forms only 1% of the body weight. Since a 40% reduction in calorigenic response to noradrenaline cannot be ascribed to the loss of an amount of brown fat constituting less than 0.5%) of the body weight, the results support the hypothesis that brown fat can mediate calorigenic responses of other tissues.

scholarly journals Glucose transporter expression and glucose utilization in skeletal muscle and brown adipose tissue during starvation and re-feeding

1992 ◽  
Vol 282 (1) ◽  
pp. 231-235 ◽  
Author(s):  
D M Smith ◽  
S R Bloom ◽  
M C Sugden ◽  
M J Holness
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Tibialis Anterior ◽  
Glucose Transporter ◽  
Glucose Utilization ◽  
Interscapular Brown Adipose Tissue ◽  
Glut 4 ◽  
Mrna Concentration ◽  
Brown Adipose ◽  
Transporter Expression

Starvation (48 h) decreased the concentration of mRNA of the insulin-responsive glucose transporter isoform (GLUT 4) in interscapular brown adipose tissue (IBAT) (56%) and tibialis anterior (10%). Despite dramatic [7-fold (tibialis anterior) and 40-fold (IBAT)] increases in glucose utilization after 2 and 4 h of chow re-feeding, no significant changes in GLUT 4 mRNA concentration were observed in these tissues over this re-feeding period. The results exclude changes in GLUT 4 mRNA concentration in mediating the responses of glucose transport in these tissues to acute re-feeding after prolonged starvation.

scholarly journals Changes in β1- and β2-adrenergic receptor mRNA levels in brown adipose tissue and heart of hypothyroid rats

1991 ◽  
Vol 277 (3) ◽  
pp. 625-629 ◽  
Author(s):  
J P Revelli ◽  
R Pescini ◽  
P Muzzin ◽  
J Seydoux ◽  
M G Fitzgerald ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Adrenergic Receptor ◽  
State Level ◽  
Total Density ◽  
Mrna Levels ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose ◽  
Displacement Experiments ◽  
Beta 2

The aim of the present work was to study the effect of hypothyroidism on the expression of the beta-adrenergic receptor (beta-AR) in interscapular brown adipose tissue and heart. The total density of plasma membrane beta-AR per tissue is decreased by 44% in hypothyroid rat interscapular brown adipose tissue and by 55% in hypothyroid rat heart compared with euthyroid controls. The effects of hypothyroidism on the density of both beta 1- and beta 2-AR subtypes were also determined in competition displacement experiments. The densities of beta 1- and beta 2-AR per tissue are decreased by 50% and 48% respectively in interscapular brown adipose tissue and by 52% and 54% in the heart. Northern blot analysis of poly(A)+ RNA from hypothyroid rat interscapular brown adipose tissue demonstrated that the levels of beta 1- and beta 2-AR mRNA per tissue are decreased by 73% and 58% respectively, whereas in hypothyroid heart, only the beta 1-AR mRNA is decreased, by 43%. The effect of hypothyroidism on the beta 1-AR mRNA is significantly more marked in the interscapular brown adipose tissue than in the heart. These results indicate that beta-AR mRNA levels are differentially regulated in rat interscapular brown adipose tissue and heart, and suggest that the decrease in beta-AR number in interscapular brown adipose tissue and heart of hypothyroid animals may in part be explained by a decreased steady-state level of beta-AR mRNA.

scholarly journals Stimulatory, but not anxiogenic, doses of caffeine act centrally to activate interscapular brown adipose tissue thermogenesis in anesthetized male rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
L. Van Schaik ◽  
C. Kettle ◽  
R. Green ◽  
W. Sievers ◽  
M. W. Hale ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Basolateral Amygdala ◽  
Free Breathing ◽  
Male Rats ◽  
Central Administration ◽  
Hypothalamic Area ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose ◽  
Brown Adipose Tissue Thermogenesis

AbstractThe role of central orexin in the sympathetic control of interscapular brown adipose tissue (iBAT) thermogenesis has been established in rodents. Stimulatory doses of caffeine activate orexin positive neurons in the lateral hypothalamus, a region of the brain implicated in stimulating BAT thermogenesis. This study tests the hypothesis that central administration of caffeine is sufficient to activate BAT. Low doses of caffeine administered either systemically (intravenous [IV]; 10 mg/kg) and centrally (intracerebroventricular [ICV]; 5–10 μg) increases BAT thermogenesis, in anaesthetised (1.5 g/kg urethane, IV) free breathing male rats. Cardiovascular function was monitored via an indwelling intra-arterial cannula and exhibited no response to the caffeine. Core temperature did not significantly differ after administration of caffeine via either route of administration. Caffeine administered both IV and ICV increased neuronal activity, as measured by c-Fos-immunoreactivity within subregions of the hypothalamic area, previously implicated in regulating BAT thermogenesis. Significantly, there appears to be no neural anxiety response to the low dose of caffeine as indicated by no change in activity in the basolateral amygdala. Having measured the physiological correlate of thermogenesis (heat production) we have not measured indirect molecular correlates of BAT activation. Nevertheless, our results demonstrate that caffeine, at stimulatory doses, acting via the central nervous system can increase thermogenesis, without adverse cardio-dynamic impact.

Organization of sympathetic innervation of interscapular brown adipose tissue in the mouse

2021 ◽  
Author(s):  
Clara Huesing ◽  
Rui Zhang ◽  
Sanjeev Gummadi ◽  
Nathan Lee ◽  
Emily Qualls‐Creekmore ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Sympathetic Innervation ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose

scholarly journals Corrigendum: Cvijic G, Lakic I, Vujovic P, Jasnic N, Djurasevic S, Dronjak-Cucakovic S, Djordjevic J. Single and combined effects of acute and chronic non-thermal stressors on rat interscapular brown adipose tissue metabolic activity. Arch biol sci. 2013;65(3):919-27 DOI:10.2298/ABS1303919C

2015 ◽  
Vol 67 (4) ◽  
pp. 1431-1431
Author(s):  
E Editorial
Keyword(s):  
Biological Sciences ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Metabolic Activity ◽  
Combined Effects ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose ◽  
New Findings ◽  
Partial Overlap ◽  
Font Color

The Editor-in-Chief has been informed that the results in Fig. 2A in the article: Single and combined effects of acute and chronic non-thermal stressors on rat interscapular brown adipose tissue metabolic activity, published in the Archives of Biological Sciences in 2013, Vol. 65, Issue 3, partially overlap with the results in Fig. 2, published in the article: Lakic I, Drenca T, Djordjevic J, Vujovic P, Jasnic N, Djurasevic S, Dronjak-Cucakovic S, Cvijic G. Arch Biol Sci. 2011;63(3):589-96, DOI:10.2298/ABS1103589L. After inspection of these articles and illustrations, and after discussion with the corresponding author of both articles, it was revealed that this is a case of partial overlap, i.e. of the authors presenting new findings that contain a comparatively small amount of previously published information. By publishing this corrigendum the journal is providing appropriate cross-referencing to the earlier work. <br><br><font color="red"><b> Link to the corrected article <u><a href="http://dx.doi.org/10.2298/ABS1303919C">10.2298/ABS1303919C</a></b></u>

Suppression of norepinephrine-induced thermogenesis in brown adipose tissue by fasting

1983 ◽  
Vol 245 (6) ◽  
pp. E582-E586 ◽  
Author(s):  
M. Hayashi ◽  
T. Nagasaka
Keyword(s):  
Adipose Tissue ◽  
Oxygen Consumption ◽  
Thyroid Hormones ◽  
Brown Adipose Tissue ◽  
Plasma Levels ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose ◽  
Colonic Temperature ◽  
Induced Changes ◽  
Thermogenic Response

Fasting-induced changes in thermogenic responses to norepinephrine (NE, 4.0 micrograms X kg-1 X min-1 iv) were studied in anesthetized rats previously cold acclimated. The rats were divided into five groups at the end of 30–40 days of cold acclimation (5 degrees C). The five groups were kept for 5 days at 25 degrees C and fed (intact fed), fasted (intact fasted), fasted with daily treatment with thyroxine (T4, 2 micrograms/kg sc), thyroidectomized and fed, or thyroidectomized and fasted. In the intact fasted group, in which the weight of brown adipose tissue decreased, NE-induced increases in oxygen consumption, colonic temperature (T col), and temperature of the interscapular brown adipose tissue (TBAT) were markedly suppressed. The two thyroidectomized groups also showed a reduction in thermogenic response. In these three groups, TBAT was lower than Tcol throughout NE infusion. In the T4-treated fasted group, fasting-induced suppression of thermogenic response to NE was largely prevented. In the intact fed and the T4-treated fasted groups, TBAT attained higher values than Tcol during NE infusion. Plasma levels of thyroid hormones were significantly lower in the intact fasted group than in the intact fed or the T4-treated fasted group. These results suggest that fasting-induced suppression of the thermogenic response to NE is largely due to the reduced thermogenic response of brown adipose tissue to NE. The lowering of the levels of the thyroid hormones induced by fasting may be one of a number of causes of the reduction in the thermogenic response of brown adipose tissue.

Effect of glucose and other hexoses on efferent discharges of brown adipose tissue nerves

1986 ◽  
Vol 251 (2) ◽  
pp. R240-R242 ◽  
Author(s):  
A. Niijima
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Sympathetic Nerves ◽  
Nerve Activity ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose ◽  
Diet Induced Thermogenesis ◽  
Effect Of Glucose

The activity of sympathetic nerves innervating interscapular brown adipose tissue of the rat was recorded. Intravenous administrations of glucose (100-300 mg/kg) enhanced the nerve activity. However, mannose, fructose, or galactose (300 mg/kg) showed no effect, suggesting the response is related to diet-induced thermogenesis in the brown adipose tissue.

Sign in / Sign up

Export Citation Format

Share Document