Nanofibers mat-based solid-phase extraction method for the pretreatment of urine samples and its application in the primary study on the disposition of nonylphenol after long-term low-level exposure in rats

2017 ◽  
Vol 1061-1062 ◽  
pp. 146-152 ◽  
Author(s):  
Liangliang Qian ◽  
Ruixian Li ◽  
Qiannan Di ◽  
Yang Shen ◽  
Qian Xu ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Extraction Method ◽  
Solid Phase ◽  
Urine Samples ◽  
Primary Study ◽  
Phase Extraction ◽  
Low Level

scholarly journals A high-throughput and sensitive methodology for the quantification of urinary 8-hydroxy-2′-deoxyguanosine: measurement with gas chromatography-mass spectrometry after single solid-phase extraction

2004 ◽  
Vol 380 (2) ◽  
pp. 541-548 ◽  
Author(s):  
Hai-Shu LIN ◽  
Andrew M. JENNER ◽  
Choon Nam ONG ◽  
Shan Hong HUANG ◽  
Matthew WHITEMAN ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Solid Phase Extraction ◽  
Healthy Subjects ◽  
Solid Phase ◽  
Large Population ◽  
Urine Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Phase Extraction ◽  
Freeze Dried ◽  
Wide Range

8-Hydroxy-2´-deoxyguanosine (8OHdG) is a widely used biomarker for the measurement of endogenous oxidative DNA damage. A sensitive method for the quantification of 8OHdG in urine by single solid-phase extraction and GC-MS (gas chromatography with MS detection) using selective ion monitoring is described in the present study. After solid-phase extraction, samples are freeze-dried, derivatized by trimethylsilylation and analysed by GC-MS. The urinary 8OHdG was quantified using heavy isotope dilution with [18O]8OHdG. The recovery of 8OHdG after the solid-phase extraction ranged from 70 to 80% for a wide range of urinary 8OHdG levels. Using 1 ml of urine, the limit of quantification was >2.5 nM (2.5 pmol/ml) and the calibration curve was linear in the range 2.5–200 nM. This method was applied to measure 8OHdG in urine samples from 12 healthy subjects. The intra- and inter-day variations were <9%. Urinary 8OHdG levels in spot urine samples from four healthy subjects were also measured for 1 week and, again, the variation was small. The presence of H2O2 in urine did not cause artifactual formation of 8OHdG. Since this assay is simple, rapid, sensitive and reproducible, it seems suitable to be used as a routine methodology for the measurement of urinary excretion of 8OHdG in large population studies.

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