Galactosylated chitosan-modified ethosomes combined with silk fibroin nanofibers is useful in transcutaneous immunization

Schwann cells (SCs) are primary structural and functional cells in peripheral nervous system and play a crucial role in peripheral nerve regeneration. Current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps and assist Scs in directing the growth of regenerating axons in nerve injury recovery. Electrospun silk fibroin nanofibers, fabricated for the cell culture in vitro, can provide such experiment support. Silk fibroin scaffolds (SFS) were fabricated with formic acid (FA), and the average fiber diameter was 305 ± 24 nm. The data from microscopic, immunohistochemical and scanning electron micrograph confirmed that the scaffold was beneficial to the adherence, proliferation and migration of SCs without exerting any significant cytotoxic effects on their phenotype. Thus, providing an experimental foundation accelerated the formation of bands of Bünger to enhance nerve regeneration. 305 nm SFS could be a candidate material for nerve tissue engineering.

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Adsorption/desorption and biofunctional properties of oleuropein loaded on different types of silk fibroin matrices

Macedonian Journal of Chemistry and Chemical Engineering ◽

10.20450/mjcce.2017.1127 ◽

2017 ◽

Vol 36 (1) ◽

Cited By ~ 1

Author(s):

Oguz Bayraktar ◽

Ali Bora Balta ◽

Guldemet Basal Bayraktar

Keyword(s):

Silk Fibroin ◽

Antibacterial Properties ◽

Average Diameter ◽

Electrospinning Technique ◽

Collector Plate ◽

Regenerated Silk Fibroin ◽

Different Types ◽

Healing Property ◽

Silk Fibroin Nanofibers ◽

Adsorption Desorption

The objective of this study was to investigate the adsorption/desorption behavior of oleuropein on different types of silk fibroin matrices including silk fibroin microfibers (MF), regenerated silk fibroin (RSF), and silk fibroin nanofibers (NF). Nanofibers with an average diameter of ranging between 24 and 326 nm were successfully prepared using the electrospinning technique. The effects of the silk fibroin concentration, the voltage applied and the distance between needle tip and collector plate on the morphology of the NF were investigated. The adsorption capacities of MF, RSF and NF were determined as 104.92, 163.07 and 228.34 mg oleuropein per gram of material, respectively. The percentage of initially adsorbed oleuropein that was desorbed was 86.08, 91.29 and 96.67% for MF, RSF and NF, respectively.NF and RSF discs loaded with oleuropein were subjected to disc diffusion assays to determine their antibacterial activity against test microorganisms Staphylococcus epidermidis (Gram +) and Escherichia coli (Gram – ). The results showed that both biomaterials possessed antibacterial properties after loading with oleuropein. Wound scratch assays using oleuropein released from NF revealed an enhancement of cell migration, indicating a wound healing property of the material.In conclusion, the NF can be utilized as a biofunctional polymeric material with better performance for the adsorption and desorption of oleuropein compared with MF and RSF.

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Incorporation of two‐dimensional nanomaterials into silk fibroin nanofibers for cardiac tissue engineering

Polymers for Advanced Technologies ◽

10.1002/pat.4765 ◽

2019 ◽

Vol 31 (2) ◽

pp. 248-259 ◽

Cited By ~ 4

Author(s):

Hojjatollah Nazari ◽

Asieh Heirani‐Tabasi ◽

Maryam Hajiabbas ◽

Masoud Khalili ◽

Mohammadhossein Shahsavari Alavijeh ◽

...

Keyword(s):

Tissue Engineering ◽

Silk Fibroin ◽

Cardiac Tissue ◽

Cardiac Tissue Engineering ◽

Two Dimensional ◽

Silk Fibroin Nanofibers

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Growth of Olfactory Ensheathing Cells on Silk Fibroin Nanofibers

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.175-176.230 ◽

2011 ◽

Vol 175-176 ◽

pp. 230-235

Author(s):

Yi Xin Shen ◽

Peng Wu ◽

Zhi Hai Fan ◽

Feng Zhang ◽

Zheng Feng Lu ◽

...

Keyword(s):

Silk Fibroin ◽

Growth Factor Receptor ◽

Olfactory Ensheathing Cells ◽

Control Group ◽

Significant Difference ◽

Contrast Microscopy ◽

Ensheathing Cells ◽

Silk Fibroin Nanofibers ◽

Experimental Group ◽

Original Characteristic

Objective: To evaluate the growth of olfactory ensheathing cells (OECs) on the silk fibroin (SF) nanofibers scaffold. Methods: The purified OECs were cultured with poly-L-lysine (control group) and 1200 nm SF nanofibers (experimental group). The morphological features and growth characteristics of which were analyzed by phase contrast microscopy. Nerve growth factor receptor (NGFR) p75 were applied to identify OECs by immunostaining. SEM was used to observe the adherence and spreading of OECs on different substrates. MTT assay was performed to evaluate the proliferation activity of OECs both on the control and experimental scaffolds. Results: The isolated OECs reached confluence after 4-5 days of culture, which were stained for antibody NGFRp75(+). The morphology of OECs on the 1200 nm SF nanofibers was similar to that on the control group. The SEM clearly revealed the close interaction between the OECs and the nanofbers. The OECs on SF nanofibers still maintain its original characteristic phenotypes. The MTT showed that the most obvious proliferation was reached over 10 days. The differences of OD values between 1200 nm SF and PLL were significant at day 5, 7 (p < 0.05). However, there was no significant difference at day 10. Conclusion: SF nanofibers scaffold could support the growth of OECs, and may be a promising tissue-engineered scaffold for the repair of SCI.

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