Chitosan/polydopamine layer by layer self-assembled silk fibroin nanofibers for biomedical applications

Self-assembled silk fibroin (SF)-polyethylenimine (PEI) multilayered films were fabricated on ethanol treated electrospun SF nanofibrous substrates via the electrostatic layer-by-layer (LBL) adsorption. The film coated membranes were characterized using scanning electron microscopy (SEM), transmission electron microscope (TEM) and X-ray photoelectron spectrophotometer (XPS). The SEM images showed that the multilayers of SF-PEI were formed on the surface of the ethanol treated SF nanofibres. The characteristics such as the fiber shape and porous structure were well maintained as the number of the coated SF-PEI bilayers was less than five. However, obvious adhesive substances and blocked pores were observed on the surface of the fibers as the number of bilayers of SF-PEI increased to six. Furthermore, the obvious core-shell structures were observed by TEM. The thickness of five SF-PEI bilayers was approximately 80nm. Additionally, the XPS results also revealed that the SF-PEI multilayer composite membranes formed. The adsorption mainly depended on a simple electrostatic interaction between the layers of SF and PEI. These SF-PEI multilayer assembled nanofibrous membranes could be a promising material for use as a sensor, gene delivery agent and scaffolds.

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Layer-by-layer self-assembled films of silk fibroin/collagen/poly (diallyldimethylammonium chloride) as nucleating surface for osseointegration to design coated dental implant materials

Materials & Design ◽

10.1016/j.matdes.2018.10.041 ◽

2018 ◽

Vol 160 ◽

pp. 1158-1167 ◽

Cited By ~ 15

Author(s):

Safitree Nawae ◽

Jirut Meesane ◽

Nantakan Muensit ◽

Chalongrat Daengngam

Keyword(s):

Dental Implant ◽

Silk Fibroin ◽

Layer By Layer ◽

Implant Materials ◽

Diallyldimethylammonium Chloride ◽

Self Assembled

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Enhanced physical and biological properties of silk fibroin nanofibers by layer-by-layer deposition of chitosan and rectorite

Journal of Colloid and Interface Science ◽

10.1016/j.jcis.2018.03.093 ◽

2018 ◽

Vol 523 ◽

pp. 208-216 ◽

Cited By ~ 41

Author(s):

Jiajia Chen ◽

Gu Cheng ◽

Rong Liu ◽

Yue Zheng ◽

Mengtian Huang ◽

...

Keyword(s):

Silk Fibroin ◽

Biological Properties ◽

Layer By Layer ◽

Layer Deposition ◽

Silk Fibroin Nanofibers

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How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Materials ◽

10.3390/ma14061510 ◽

2021 ◽

Vol 14 (6) ◽

pp. 1510

Author(s):

Sylwia Grabska-Zielińska ◽

Alina Sionkowska

Keyword(s):

Silk Fibroin ◽

Biomedical Applications ◽

Magnetic Particles ◽

Chemical Properties ◽

Ternary Mixtures ◽

Cross Linking ◽

Advantages And Disadvantages ◽

Physico Chemical ◽

Binary And Ternary Mixtures ◽

To Receive

This review supplies a report on fresh advances in the field of silk fibroin (SF) biopolymer and its blends with biopolymers as new biomaterials. The review also includes a subsection about silk fibroin mixtures with synthetic polymers. Silk fibroin is commonly used to receive biomaterials. However, the materials based on pure polymer present low mechanical parameters, and high enzymatic degradation rate. These properties can be problematic for tissue engineering applications. An increased interest in two- and three-component mixtures and chemically cross-linked materials has been observed due to their improved physico-chemical properties. These materials can be attractive and desirable for both academic, and, industrial attention because they expose improvements in properties required in the biomedical field. The structure, forms, methods of preparation, and some physico-chemical properties of silk fibroin are discussed in this review. Detailed examples are also given from scientific reports and practical experiments. The most common biopolymers: collagen (Coll), chitosan (CTS), alginate (AL), and hyaluronic acid (HA) are discussed as components of silk fibroin-based mixtures. Examples of binary and ternary mixtures, composites with the addition of magnetic particles, hydroxyapatite or titanium dioxide are also included and given. Additionally, the advantages and disadvantages of chemical, physical, and enzymatic cross-linking were demonstrated.

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Comparative Study of Silk Fibroin-Based Hydrogels and Their Potential as Material for 3-Dimensional (3D) Printing

Molecules ◽

10.3390/molecules26133887 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3887

Author(s):

Watcharapong Pudkon ◽

Chavee Laomeephol ◽

Siriporn Damrongsakkul ◽

Sorada Kanokpanont ◽

Juthamas Ratanavaraporn

Keyword(s):

3D Printing ◽

Silk Fibroin ◽

Biomedical Applications ◽

Mechanical Stability ◽

Three Dimensional ◽

Structure Stability ◽

3 Dimensional ◽

Critical Technology ◽

Box Structure ◽

High Degree

Three-dimensional (3D) printing is regarded as a critical technology in material engineering for biomedical applications. From a previous report, silk fibroin (SF) has been used as a biomaterial for tissue engineering due to its biocompatibility, biodegradability, non-toxicity and robust mechanical properties which provide a potential as material for 3D-printing. In this study, SF-based hydrogels with different formulations and SF concentrations (1–3%wt) were prepared by natural gelation (SF/self-gelled), sodium tetradecyl sulfate-induced (SF/STS) and dimyristoyl glycerophosphorylglycerol-induced (SF/DMPG). From the results, 2%wt SF-based (2SF) hydrogels showed suitable properties for extrusion, such as storage modulus, shear-thinning behavior and degree of structure recovery. The 4-layer box structure of all 2SF-based hydrogel formulations could be printed without structural collapse. In addition, the mechanical stability of printed structures after three-step post-treatment was investigated. The printed structure of 2SF/STS and 2SF/DMPG hydrogels exhibited high stability with high degree of structure recovery as 70.4% and 53.7%, respectively, compared to 2SF/self-gelled construct as 38.9%. The 2SF/STS and 2SF/DMPG hydrogels showed a great potential to use as material for 3D-printing due to its rheological properties, printability and structure stability.

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Oxygen Permeability of Silk Fibroin Hydrogels and Their Use as Materials for Contact Lenses: A Purposeful Analysis

Gels ◽

10.3390/gels7020058 ◽

2021 ◽

Vol 7 (2) ◽

pp. 58

Author(s):

Traian V. Chirila

Keyword(s):

Tissue Engineering ◽

Bombyx Mori ◽

Silk Fibroin ◽

Biomedical Applications ◽

Oxygen Permeability ◽

Contact Lenses ◽

The Past ◽

Fibrous Protein ◽

Silk Moth ◽

Bombyx Mori Silk

Fibroin is a fibrous protein that can be conveniently isolated from the silk cocoons produced by the larvae of Bombyx mori silk moth. In its form as a hydrogel, Bombyx mori silk fibroin (BMSF) has been employed in a variety of biomedical applications. When used as substrates for biomaterial-cells constructs in tissue engineering, the oxygen transport characteristics of the BMSF membranes have proved so far to be adequate. However, over the past three decades the BMSF hydrogels have been proposed episodically as materials for the manufacture of contact lenses, an application that depends on substantially elevated oxygen permeability. This review will show that the literature published on the oxygen permeability of BMSF is both limited and controversial. Additionally, there is no evidence that contact lenses made from BMSF have ever reached commercialization. The existing literature is discussed critically, leading to the conclusion that BMSF hydrogels are unsuitable as materials for contact lenses, while also attempting to explain the scarcity of data regarding the oxygen permeability of BMSF. To the author’s knowledge, this review covers all publications related to the topic.

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Layer-by-Layer Self-Assembly Composite Coatings for Improved Corrosion and Wear Resistance of Mg Alloy for Biomedical Applications

Coatings ◽

10.3390/coatings11050515 ◽

2021 ◽

Vol 11 (5) ◽

pp. 515

Author(s):

Tongfang Liu ◽

Song Rui ◽

Sheng Li

Keyword(s):

Wear Resistance ◽

Self Assembly ◽

Biomedical Applications ◽

Composite Coatings ◽

Covalent Bond ◽

Mg Alloys ◽

Mg Alloy ◽

Layer By Layer ◽

Corrosion Propagation ◽

Chemical Linkage

Mg alloys are promising biomedical metal due to their natural degradability, good processability, and favorable mechanical properties. However, the poor corrosion resistance limits their further clinical applications. In this study, the combined strategies of surface chemical treatment and layer-by-layer self-assembly were used to prepare composite coatings on Mg alloys to improve the biocorrosion resistance. Specially, alkalized AZ91 Mg alloy generated chemical linkage with silane via Si–O–Mg covalent bond at the interface. Subsequently, Si–OH group from silane formed a crosslinked silane layer by Si–O–Si network. Further chemical assembly with graphene oxide (GO), lengthened the diffusion pathway of corrosive medium. The chemically assembled composite coatings could firmly bond to Mg alloy substrate, which persistently and effectively acted as compact barriers against corrosion propagation. Improved biocorrosion resistance of AZ91 Mg alloy with self-assembly composite coatings of silane/GO was subsequently confirmed by immersion tests. Besides, the Mg alloy exhibited good wear resistance due to outside layer of GO with a lubricant effect. Cell viability of higher than 75% had also been found for the alloy with self-assembly composite coatings, which showed good cytocompatibility.

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