Experimental Study of the Pathogenicity of Pasteurella multocida Capsular Type B in Rabbits

2015 ◽  
Vol 153 (2-3) ◽  
pp. 160-166 ◽  
Author(s):  
S. Katoch ◽  
L. Verma ◽  
M. Sharma ◽  
R.K. Asrani ◽  
S. Kumar ◽  
...  
Keyword(s):  
Experimental Study ◽  
Pasteurella Multocida ◽  
Capsular Type ◽  
Type B

scholarly journals Protective efficacy of calcium phosphate nanoparticle adsorbed bivalent subunit vaccine of Pasteurella multocida against homologous challenge in mice

2020 ◽  
Author(s):  
Songyukta Shyam ◽  
Shantanu Tamuly ◽  
Probodh Borah ◽  
Rajeev Kumar Sharma
Keyword(s):  
Calcium Phosphate ◽  
Subunit Vaccine ◽  
Pasteurella Multocida ◽  
Protective Efficacy ◽  
Economic Loss ◽  
Capsular Type ◽  
Significant Protection ◽  
Type B ◽  
Serotype A ◽  
Calcium Phosphate Nanoparticle

ABSTRACTSwine pasteurellosis, caused by Pasteurella multocida capsular types A and D, causes heavy economic loss to the pig farmers. The vaccine presently used is abacterin of Pasteurella multocida capsular type B that is proven to be effective against bovine pasteurellosis. However, its efficacy against swine pasteurellosis is questionable. The present study was carried out to evaluate the efficacy of calcium phosphate nanoparticle adjuvanted bivalent subunit vaccine prepared from Pasteurella multocida capsular types A and D along with a monovalent subunit vaccine prepared from Pasteurella multocida capsular type B in mice. The Alum precipitated bacterin vaccine was used as the control. The bivalent subunit vaccine comprising the immune components of both the capsular types showed significantly higher IgG response than either of the other two vaccines. Both the calcium phosphate nanoparticle adjuvanted vaccines could elicit 100% protection in mice against homologous challenges but the aluminum hydroxide adjuvanted bacterin vaccine could not elicit significant protection. Based on this preliminary work, it was concluded that the bivalent subunit vaccine would be a better option for immunization of swine against swine pasteurellosis.IMPORTANCE OF THE WORKThe swine pasteurellosis is an important economic disease affecting the pig population in the North-eastern part of India that contributes the major pig population. The disease is caused by Serotype A and D of Pasteurella multocida. At present the inactivated vaccine is used that is actually developed against P52 strain of serotype B:2 of Pasteurella multocida, which is mainly involved in haemorrhagic septicaemia (or bovine pasteurellosis) that affects the cattle, buffaloe, sheep and goat. As a result, the present vaccine does not give sufficient protection in pigs but gives significant protection in cattle, buffaloe, sheep and goat. Hence, there is a need of development of vaccine that can address specifically swine pasteurellosis by targeting serotype A and D of Pasteurella multocida.

Outbreaks of pleuritis and peritonitis in calves associated with Pasteurella multocida capsular type B strain

2011 ◽  
Vol 59 (1) ◽  
pp. 40-45 ◽  
Author(s):  
AMJ McFadden ◽  
H Christensen ◽  
RA Fairley ◽  
FI Hill ◽  
JM Gill ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Capsular Type ◽  
Type B

scholarly journals Identification and Comparison of Hyaluronic Acid Biosynthetic Genes from Different Capsular Types of Pasteurella Multocida

2020 ◽  
Author(s):  
TEERASAK E-KOBON ◽  
Pailin Pasomboon ◽  
Pramote Chumnanpuen
Keyword(s):  
Hyaluronic Acid ◽  
Pasteurella Multocida ◽  
Type A ◽  
Capsular Type ◽  
Type B ◽  
Biosynthetic Genes ◽  
Bioinformatics Analyses ◽  
Molecular Comparison ◽  
Species Similarity ◽  
High Level

Abstract BackgroundPasteurella multocida produces a capsule composed of different polysaccharides according to the capsular serotype (A, B, D, E, and F). Hyaluronic acid (HA) is a component of certain capsular types of this bacterium, especially capsular type A. Previously, two HA biosynthetic genes from a capsular type A strain were studied for the industrial-scale improvement of HA production. Molecular comparison of these genes across different capsular serotypes of P. multocida has not been reported. This study aimed to compare nine HA biosynthetic genes (glck, pgi, pgm, galU, hyaC, glmS, glmM, glmU, and hyaD) of eleven P. multocida strains (A:B:D:F = 8:1:1:1) with those of other organisms using sequence and structural bioinformatics analyses.ResultsThese nine genes showed a high level of within-species similarity (98–99%) compared to other organisms. Only the last gene of two strains with capsular type A:3 (PM70 and CRIMBP-0884) and one capsular type F strain (HN07) significantly differed from those of other strains (82%). Analysis of amino acid patterns together with phylogenetic results showed that the HA biosynthetic genes of the type A and D strains were closely related compared to those of the type B and F strains. However, the genes in the capsular type F strain were notably similar to those of the capsular type A:3 strain. Protein structural analysis supported structural similarities of the encoded enzymes between the strains of capsular types A, B, D, and F, except for the Glck, Pgm, GlmU and HyaD proteins.ConclusionOur bioinformatics analyses proposed that variations observed within these genes could be useful for genetic engineering-based improvement of hyaluronic acid production.

Study on nitrified liquor recycling process operations using polyurethane foam sponge cubes as a biomass support medium

1994 ◽  
Vol 30 (6) ◽  
pp. 143-149 ◽  
Author(s):  
H. Deguchi ◽  
M. Kashiwaya
Keyword(s):  
Experimental Study ◽  
Polyurethane Foam ◽  
Type A ◽  
Denitrification Rate ◽  
Rate Coefficients ◽  
Type B ◽  
Recycling Process ◽  
Support Medium ◽  
Suspended Growth ◽  
Nitrification And Denitrification

An experimental study was carried out to find a way of using sponge cubes as a biomass support medium to reaction tanks for nitrified liquor recycling process. Type-A, in which biomass fixed cubes are contacted with both anoxic and oxic stages and Type-B, where biomass fixed cubes are contacted with either anoxic or oxic stages, were selected as experimental cases. The results showed that the amount of CO2-C generated which was related to sludge production for Type-B exceeded the amount for Type-A by between 12 to 21%. The nitrification and denitrification rate coefficients at 20°C in Type-A were 1.5 and 1.6 times respectively higher than the coefficients for suspended growth. The rate coefficients in Type-B were 1.5 and 2.0 times respectively higher than the coefficients for Type-A.

scholarly journals Comparative Genomics Analysis of Two Different Virulent Bovine Pasteurella multocida Isolates

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Huihui Du ◽  
Rendong Fang ◽  
Tingting Pan ◽  
Tian Li ◽  
Nengzhang Li ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Genome Sequence ◽  
Pasteurella Multocida ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Insertion Sequences ◽  
Capsular Type ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Comparative Genomics Analysis

The Pasteurella multocida capsular type A isolates can cause pneumonia and bovine respiratory disease (BRD). In this study, comparative genomics analysis was carried out to identify the virulence genes in two different virulent P. multocida capsular type A isolates (high virulent PmCQ2 and low virulent PmCQ6). The draft genome sequence of PmCQ2 is 2.32 Mbp and contains 2,002 protein-coding genes, 9 insertion sequence (IS) elements, and 1 prophage region. The draft genome sequence of PmCQ6 is 2.29 Mbp and contains 1,970 protein-coding genes, 2 IS elements, and 3 prophage regions. The genome alignment analysis revealed that the genome similarity between PmCQ2 and PmCQ6 is 99% with high colinearity. To identify the candidate genes responsible for virulence, the PmCQ2 and PmCQ6 were compared together with that of the published genomes of high virulent Pm36950 and PmHN06 and avirulent Pm3480 and Pm70 (capsular type F). Five genes and two insertion sequences are identified in high virulent strains but not in low virulent or avirulent strains. These results indicated that these genes or insertion sequences might be responsible for the virulence of P. multocida, providing prospective candidates for further studies on the pathogenesis and the host-pathogen interactions of P. multocida.

scholarly journals Molecular identification by multiplex polymerase chain reaction of Pasteurella multocida in cattle and buffaloes in Baghdad

2014 ◽  
Vol 38 (1) ◽  
pp. 99-106
Author(s):  
Ihab G. M. AL-Shemmari
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Multiplex Polymerase Chain Reaction ◽  
Pasteurella Multocida ◽  
Type B ◽  
Chain Reaction ◽  
Blood Samples ◽  
Nasal Swabs ◽  
Polymerase Chain ◽  
B 31

The aim of this study was to identify pasteurella multocida and their types by PCR in cattle’s and buffaloesi bagdad from March to August 2012 on 204 animals , including 102 cattle and 102 buffaloes at slaughter houses from Baghdad .Blood samples and nasal swaps were collected , before slaughtering and lung tissues of slaughtered animal , and from 54 clinically suspected cases of pasteurellosis , including 27 bovines ,and 27 buffaloes the samples taken included blood and nasal swabs . Pasteurellamultocida were isolated from 94 animals include 49 cattle 45 buffaloes. The typing of the isolates by multiplex PCR for genotyping Pasteuerllamultocida revealed 93 isolates of type B , 31 from cattle and 62 from buffaloes ,and 81 isolates of type A , 55 from cattle and 26 from buffaloes .

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