Protective efficacy of calcium phosphate nanoparticle adsorbed bivalent subunit vaccine of Pasteurella multocida against homologous challenge in mice

ABSTRACTSwine pasteurellosis, caused by Pasteurella multocida capsular types A and D, causes heavy economic loss to the pig farmers. The vaccine presently used is abacterin of Pasteurella multocida capsular type B that is proven to be effective against bovine pasteurellosis. However, its efficacy against swine pasteurellosis is questionable. The present study was carried out to evaluate the efficacy of calcium phosphate nanoparticle adjuvanted bivalent subunit vaccine prepared from Pasteurella multocida capsular types A and D along with a monovalent subunit vaccine prepared from Pasteurella multocida capsular type B in mice. The Alum precipitated bacterin vaccine was used as the control. The bivalent subunit vaccine comprising the immune components of both the capsular types showed significantly higher IgG response than either of the other two vaccines. Both the calcium phosphate nanoparticle adjuvanted vaccines could elicit 100% protection in mice against homologous challenges but the aluminum hydroxide adjuvanted bacterin vaccine could not elicit significant protection. Based on this preliminary work, it was concluded that the bivalent subunit vaccine would be a better option for immunization of swine against swine pasteurellosis.IMPORTANCE OF THE WORKThe swine pasteurellosis is an important economic disease affecting the pig population in the North-eastern part of India that contributes the major pig population. The disease is caused by Serotype A and D of Pasteurella multocida. At present the inactivated vaccine is used that is actually developed against P52 strain of serotype B:2 of Pasteurella multocida, which is mainly involved in haemorrhagic septicaemia (or bovine pasteurellosis) that affects the cattle, buffaloe, sheep and goat. As a result, the present vaccine does not give sufficient protection in pigs but gives significant protection in cattle, buffaloe, sheep and goat. Hence, there is a need of development of vaccine that can address specifically swine pasteurellosis by targeting serotype A and D of Pasteurella multocida.

Download Full-text

Comparative Evaluation of Humoral Immune Response Generated by Calcium Phosphate Nanoparticle Adjuvanted and Saponin-Adjuvanted Recombinant Outermembrane Protein 87 (Omp87) of Pasteurella multocida (Serotype B:2) in Mice

Journal of Nanopharmaceutics and Drug Delivery ◽

10.1166/jnd.2014.1045 ◽

2014 ◽

Vol 2 (1) ◽

pp. 80-86

Author(s):

Shantanu Tamuly ◽

Mumtesh Kumar Saxena ◽

Anjani Saxena ◽

Rajesh Kumar Mishra ◽

Richa Jha

Keyword(s):

Immune Response ◽

Calcium Phosphate ◽

Humoral Immune Response ◽

Comparative Evaluation ◽

Pasteurella Multocida ◽

Humoral Immune ◽

Serotype B ◽

Calcium Phosphate Nanoparticle

Download Full-text

Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

Veterinary World ◽

10.14202/vetworld.2019.434-439 ◽

2019 ◽

Vol 12 (3) ◽

pp. 434-439

Author(s):

Fernando A. Bessone ◽

Maria Laura Soriano Perez ◽

Gustavo Zielinski ◽

Marina Dibarbora ◽

M. B. Conde ◽

...

Keyword(s):

Antibiotic Susceptibility ◽

Pasteurella Multocida ◽

Type A ◽

Economic Loss ◽

Diffusion Method ◽

Atrophic Rhinitis ◽

Specific Gene ◽

Capsular Type ◽

Progressive Atrophic Rhinitis ◽

Susceptibility Profiles

Background: Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry. Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains. Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles. Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF. Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.

Download Full-text

Outbreaks of pleuritis and peritonitis in calves associated with Pasteurella multocida capsular type B strain

New Zealand Veterinary Journal ◽

10.1080/00480169.2011.547168 ◽

2011 ◽

Vol 59 (1) ◽

pp. 40-45 ◽

Cited By ~ 15

Author(s):

AMJ McFadden ◽

H Christensen ◽

RA Fairley ◽

FI Hill ◽

JM Gill ◽

...

Keyword(s):

Pasteurella Multocida ◽

Capsular Type ◽

Type B

Download Full-text

Experimental Study of the Pathogenicity of Pasteurella multocida Capsular Type B in Rabbits

Journal of Comparative Pathology ◽

10.1016/j.jcpa.2015.06.005 ◽

2015 ◽

Vol 153 (2-3) ◽

pp. 160-166 ◽

Cited By ~ 5

Author(s):

S. Katoch ◽

L. Verma ◽

M. Sharma ◽

R.K. Asrani ◽

S. Kumar ◽

...

Keyword(s):

Experimental Study ◽

Pasteurella Multocida ◽

Capsular Type ◽

Type B

Download Full-text

Identification and Comparison of Hyaluronic Acid Biosynthetic Genes from Different Capsular Types of Pasteurella Multocida

10.21203/rs.3.rs-22706/v1 ◽

2020 ◽

Author(s):

TEERASAK E-KOBON ◽

Pailin Pasomboon ◽

Pramote Chumnanpuen

Keyword(s):

Hyaluronic Acid ◽

Pasteurella Multocida ◽

Type A ◽

Capsular Type ◽

Type B ◽

Biosynthetic Genes ◽

Bioinformatics Analyses ◽

Molecular Comparison ◽

Species Similarity ◽

High Level

Abstract BackgroundPasteurella multocida produces a capsule composed of different polysaccharides according to the capsular serotype (A, B, D, E, and F). Hyaluronic acid (HA) is a component of certain capsular types of this bacterium, especially capsular type A. Previously, two HA biosynthetic genes from a capsular type A strain were studied for the industrial-scale improvement of HA production. Molecular comparison of these genes across different capsular serotypes of P. multocida has not been reported. This study aimed to compare nine HA biosynthetic genes (glck, pgi, pgm, galU, hyaC, glmS, glmM, glmU, and hyaD) of eleven P. multocida strains (A:B:D:F = 8:1:1:1) with those of other organisms using sequence and structural bioinformatics analyses.ResultsThese nine genes showed a high level of within-species similarity (98–99%) compared to other organisms. Only the last gene of two strains with capsular type A:3 (PM70 and CRIMBP-0884) and one capsular type F strain (HN07) significantly differed from those of other strains (82%). Analysis of amino acid patterns together with phylogenetic results showed that the HA biosynthetic genes of the type A and D strains were closely related compared to those of the type B and F strains. However, the genes in the capsular type F strain were notably similar to those of the capsular type A:3 strain. Protein structural analysis supported structural similarities of the encoded enzymes between the strains of capsular types A, B, D, and F, except for the Glck, Pgm, GlmU and HyaD proteins.ConclusionOur bioinformatics analyses proposed that variations observed within these genes could be useful for genetic engineering-based improvement of hyaluronic acid production.

Download Full-text

Immunogenicity and Protective Efficacy of Iron-inactivated Pasteurella multocida A:1 Vaccine against Fowl Cholera in Backyard Chicken

Indian Journal of Animal Research ◽

10.18805/ijar.b-4432 ◽

2021 ◽

Author(s):

M. Abhilash ◽

T.R. Kannaki ◽

E. Priyanka ◽

Santosh Haunshi

Keyword(s):

Pasteurella Multocida ◽

Protective Efficacy ◽

Economic Loss ◽

Bacterial Disease ◽

Oil Emulsion ◽

Fowl Cholera ◽

Antibody Titers ◽

Mortality Percentage ◽

Backyard Chicken ◽

Significant Economic Loss

Background: Fowl cholera is a highly fatal, contagious bacterial disease that incurs significant economic loss in commercial as well as back-yard poultry. Vaccination is the most effective way in controlling this disease. In this study, we prepared and evaluated the immunogenicity of iron- inactivated Pasteurella multocida A:1 vaccine and its protective efficacy against fowl cholera experimental infection in backyard chicken.Methods: Scaled up Pasteurella multocida A:1 culture with 5 X 108 CFU/ml equivalent to 2.5 mg of antigen per dose was used for preparation of experimental vaccines. Formalin inactivated and mixed with APS adjuvant (FIA), formalin inactivated-Freund adjuvant (FIF), Iron inactivated and adjuvanted with iron (III), Iron inactivated from iron supplemented media and adjuvanted with iron (ISII) and commercial oil emulsion vaccine (CV) were used in the study. A total of 120 Vanaraja birds (n=20/group) of 2 weeks age were immunized with these vaccine and booster were given at 3rd and 6th week with respective vaccine. Specific antibody titers were assessed by iELISA in the serum at weekly intervals. The birds were challenged (n=6 /group) with 5x104 CFU/ml of virulent isolate by intraperitoneal route and morbidity, mortality percentage were observed.Result: Protective antibody titers were induced by iron inactivated vaccine from 4th week of immunization and upon booster doses it induced significantly higher (P less than 0.05) antibody response. The iron inactivated experimental vaccine gave equivalent protection as that of commercial vaccine upon challenge infection.

Download Full-text

Immune Protective Efficacy of China’s Traditional Inactivated and Attenuated Vaccines against the Prevalent Strains of Pasteurella multocida in Mice

Vaccines ◽

10.3390/vaccines9101155 ◽

2021 ◽

Vol 9 (10) ◽

pp. 1155

Author(s):

Li-Jun Guan ◽

Ji-Jian Song ◽

Yun Xue ◽

Xia Ai ◽

Zhi-Jun Liu ◽

...

Keyword(s):

Mouse Model ◽

Protective Effect ◽

Aluminum Hydroxide ◽

Pasteurella Multocida ◽

Protective Efficacy ◽

Cross Protection ◽

Immune Protection ◽

Capsular Type ◽

Protection Effect ◽

Antibody Titers

Capsular type A and D strains of Pasteurella multocida are the main epidemic serogroups in pigs in China. In this study, we preliminarily evaluated the immune protective efficacy of the two traditional vaccines, an inactivated C44-1 aluminum-hydroxide-gel-adjuvanted (Alh–C44-1) vaccine and a live EO630 vaccine, against currently circulating strains of P. multocida in a mouse model. Mice immunized twice with conventional vaccines generated higher antibody titers, and significantly higher levels of IgG were observed in the mice inoculated with the inactivated Alh–C44-1 vaccine on day 35 (p < 0.05) than those with the live EO630 vaccine. The mice immune protection test showed that the vaccination groups had a 57% or 71% protection effect against the serogroup B strain, but had no protective effect against epidemic strains. In conclusion, our study found that the widely used traditional P. multocida vaccines in China provide good protection against homologous strains, but could not provide cross-protection against heterologous strains in a mouse model.

Download Full-text