The relationship between the type of ovarian cyst and endometritis in Holstein dairy cows

2019 ◽  
Vol 166 ◽  
pp. 135
Author(s):  
A. Khodakaram-Tafti ◽  
M. Kafi ◽  
M. Heidari
2020 ◽  
Vol 90 (6) ◽  
pp. 557-563
Author(s):  
Reza Asadpour ◽  
◽  
Logman Feyzi ◽  
Razi Jafari-Joozani ◽  
Hossain Hamali ◽  
...  

Endometritis is one of the reproductive diseases that can cause disturbances of postpartum uterine health in cattle. Therefore, identification of resistant genotypes to endometritis is essential. The objective of this study was to evaluate the association between the single nucleotide polymorphism in the interleukin-8 receptor-α (CXCR1) gene and the possibility of endometritis in Holstein dairy cows. For this purpose, blood samples were collected from multiparous dairy cows with a history of clinical endometritis (n = 30), and normal, healthy cows as the control group (n = 10). Deoxyribonucleic acid (DNA) was isolated from a blood sample. To determine genotype, the polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP) was used. The results indicated the presence of a different proportion of polymorphisms (G > C) in the CXCR1 gene in cows with clinical endometritis, compared to the control group. Statistical analyses showed that there is a significant correlation between the incidence of the disease and the CXCR1 genotype in nucleotide position 956. The incidence rate of clinical endometritis was associated with the CXCR1.956 genotype; cows with genotype GC had a higher incidence of clinical endometritis compared with cows with the GG genotype. Overall, the results showed that CXCR1 polymorphism could be a useful marker for identification of resistant genotypes to endometritis in Holstein dairy cows.


Author(s):  
Martina Doležalová ◽  
Luděk Stádník ◽  
Michaela Nejdlová ◽  
Dana Němečková ◽  
Jan Beran ◽  
...  

The objective of this research was to evaluate the relationship between the course and the depth of negative energy balance (NEB) rated by changes of the body condition after calving and subsequent recovery of reproductive abilities of Holstein dairy cows treated by OVSYNCH. The body condition was evaluated by the BCS system one week before calving and subsequently at 30-day intervals for the period of 6 months of lactation. Recovery of ovarian functions was evaluated by the results of ultrasound examination of all the dairy cows’ ovaries in two controls after calving. The first one was performed on an average of 67 days after calving, and the second at a 60-day interval. The occurrence of individual findings - the presence of corpus luteum, ovarian cysts or ovaries without findings was evaluated as an effect of NEB on the recovery of the ovarian cycle of dairy cows and their subsequent possibility of conception. The suitable cows were treated by the OVSYNCH system after the 1st and 2nd ultrasound examination. Therefore, the insemination interval was calculated and in the case of dairy cows, pregnancy detection, also the number of services per conception and the length of open days. Twice performed hormonal treatment of 73.6% of the animals delayed the period by 50.2 days. In all, 202 dairy cows calved in the period from 29th July 2011 to 7th February 2012 were included in this observation. Significant effects of the body condition change on the ovarian activity as documented by sonographic examination and reproductive indicators appeared primarily in the second month of lactation. The best reproductive indicators were found in cows with the lowest body condition change, thus with a small decline or even increase of body condition score (−0.0 to +0.75 points, P < 0.05). On the contrary, the worst level of indicators of recovery of the reproduction functions were documented in the dairy cows with the most marked decline of BCS (P < 0.05).


Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 722
Author(s):  
Jang-Hoon Jo ◽  
Jalil Ghassemi Nejad ◽  
Dong-Qiao Peng ◽  
Hye-Ran Kim ◽  
Sang-Ho Kim ◽  
...  

This study aims to characterize the influence of short-term heat stress (HS; 4 day) in early lactating Holstein dairy cows, in terms of triggering blood metabolomics and parameters, milk yield and composition, and milk microRNA expression. Eight cows (milk yield = 30 ± 1.5 kg/day, parity = 1.09 ± 0.05) were homogeneously housed in environmentally controlled chambers, assigned into two groups with respect to the temperature humidity index (THI) at two distinct levels: approximately ~71 (low-temperature, low-humidity; LTLH) and ~86 (high-temperature, high-humidity; HTHH). Average feed intake (FI) dropped about 10 kg in the HTHH group, compared with the LTLH group (p = 0.001), whereas water intake was only numerically higher (p = 0.183) in the HTHH group than in the LTLH group. Physiological parameters, including rectal temperature (p = 0.001) and heart rate (p = 0.038), were significantly higher in the HTHH group than in the LTLH group. Plasma cortisol and haptoglobin were higher (p < 0.05) in the HTHH group, compared to the LTLH group. Milk yield, milk fat yield, 3.5% fat-corrected milk (FCM), and energy-corrected milk (ECM) were lower (p < 0.05) in the HTHH group than in the LTLH group. Higher relative expression of milk miRNA-216 was observed in the HTHH group (p < 0.05). Valine, isoleucine, methionine, phenylalanine, tyrosine, tryptophan, lactic acid, 3-phenylpropionic acid, 1,5-anhydro-D-sorbitol, myo-inositol, and urea were decreased (p < 0.05). These results suggest that early lactating cows are more vulnerable to short-term (4 day) high THI levels—that is, HTHH conditions—compared with LTLH, considering the enormous negative effects observed in measured blood metabolomics and parameters, milk yield and compositions, and milk miRNA-216 expression.


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