scholarly journals The relationship between polymorphism of the CXCR1 gene and the risk of endometritis in Holstein dairy cows

2020 ◽  
Vol 90 (6) ◽  
pp. 557-563
Author(s):  
Reza Asadpour ◽  
◽  
Logman Feyzi ◽  
Razi Jafari-Joozani ◽  
Hossain Hamali ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Control Group ◽  
Nucleotide Position ◽  
Single Nucleotide ◽  
Uterine Health ◽  
Pcr Rflp ◽  
History Of ◽  
The Relationship ◽  
Resistant Genotypes ◽  
Holstein Dairy Cows

Endometritis is one of the reproductive diseases that can cause disturbances of postpartum uterine health in cattle. Therefore, identification of resistant genotypes to endometritis is essential. The objective of this study was to evaluate the association between the single nucleotide polymorphism in the interleukin-8 receptor-α (CXCR1) gene and the possibility of endometritis in Holstein dairy cows. For this purpose, blood samples were collected from multiparous dairy cows with a history of clinical endometritis (n = 30), and normal, healthy cows as the control group (n = 10). Deoxyribonucleic acid (DNA) was isolated from a blood sample. To determine genotype, the polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP) was used. The results indicated the presence of a different proportion of polymorphisms (G > C) in the CXCR1 gene in cows with clinical endometritis, compared to the control group. Statistical analyses showed that there is a significant correlation between the incidence of the disease and the CXCR1 genotype in nucleotide position 956. The incidence rate of clinical endometritis was associated with the CXCR1.956 genotype; cows with genotype GC had a higher incidence of clinical endometritis compared with cows with the GG genotype. Overall, the results showed that CXCR1 polymorphism could be a useful marker for identification of resistant genotypes to endometritis in Holstein dairy cows.

The association between allergic rhinitis and Polymorphism of Toll like receptors 2 & 4 genes

2021 ◽  
Vol 30 (3) ◽  
pp. 37-42
Author(s):  
Abdelhakim F. Ghallab ◽  
Rasha A. El sayed ◽  
Mostafa G. Sobhy
Keyword(s):  
Allergic Rhinitis ◽  
University Hospital ◽  
Control Group ◽  
P Value ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Atopic State ◽  
Major Allele ◽  
Polymerase Chain ◽  
The Relationship

Background: Allergic rhinitis is atopic disorder, 10% to 25% of the population worldwide are suffering from it, The prevalence is increasing during the last 10 years. Objectives: To study the relationship among polymorphism of single nucleotide in TLR2 and TLR4 genes and the risk of allergic rhinitis disease. Methodology: This study was done on 60 patients suffering from allergic rhinitis and 30 healthy subjects as a control group from April 2019 to March 2020. The patients were collected from Otorhinolaryngology Department of Benha University Hospital. Test of Skin prick (SPT) was done to assess atopic state. Blood samples were collected to detect TLR gene polymorphism by Polymerase chain reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Results: The genotypic frequencies of TLR2 Arg753Gln showed increased frequency of the homozygous (GG) genotype among the controls (80%) more than the allergic rhinitis patients (30%). The heterozygous (AG) genotype was increased among the allergic rhinitis patients (62.5%) more than in the healthy group (15%) with OR =9.4, 95% CI (2.4-37.7) and significant P-value. Also, the homozygous mutant (AA) genotype has more trend in the patients (7.5%) than in the control subjects (5%), with OR = 0. 6, 95% CI (0.1-6.7) and non-significant P-value. The genotypic frequencies Statistical data in TLR4 Asp299Gly revealed that the homozygous (AA) genotype has more frequency in the controls (70%) than the allergic rhinitis patients (20%). The heterozygous (AG) genotype was more prevalent among the allergic rhinitis patients (65%) than the controls (30%) with OR =4.3, 95% CI (1.4-13.8) and significant P-value. Conclusion: GG genotype of TLR2 and AA genotype of TLR4 are least affected by allergic rhinitis disease and the major allele in both gene is protective against the disease.

scholarly journals An Investigation of the Relationship between Involvement Sites in Vitiligo Patients and Autoimmune Diseases and Hematological Parameters

2019 ◽  
Vol 4 (10) ◽  
Author(s):  
Ebru KARAGUN
Keyword(s):  
Hematological Parameters ◽  
Fasting Blood Glucose ◽  
Unknown Etiology ◽  
Control Group ◽  
Endocrine Disorders ◽  
Positive Correlation ◽  
Lymphocyte Ratio ◽  
History Of ◽  
The Mean ◽  
The Relationship

Aim-Objectives: Vitiligo is an acquired idiopathic disease which progresses with melanocyte destruction and is clinically characterized by depigmented lesions of unknown etiology. Vitiligo may be coexistence with a autoimmune and endocrine disorders. This study examined the sT3, fT4, TSH, Anti-TPO, Anti-Tg, Vitamin B12 and fasting blood glucose (FBG) values, and thrombocyte-to-lymphocyte ratio(TLR), neutrophil-to-lymphocyte ratio(NLR), the mean platelet volume(MPV) the correlation of depigmented lesions with the extent of body involvement sites(IS). Materials and Method: The study enrolled 67 patients aged 0‒65 who were diagnosed with non-segmental generalized vitiligo and in whom an increase in lesions had been observed in the last six months. The IS of the lesions in the patients were evaluated as IS ˂10%(1st group), 10%‒20% (2nd group), 20% - 30%(3rd group), 30% - 40%(4th group), 40% ‒50 %(5th group), and ˃50%(6th group). The control group consisted of patients who had presented to the outpatient clinic having had no history of vitiligo detected in themselves nor in their families. Results: No significant correlation was found between IS and sT3, fT4, TSH, Anti-TPO, Anti-Tg, Vit. B12, PBG or MPV. A moderately positive correlation was found between IS and duration (p <0.05) and a mildly positive correlation between IS and NLR and TLR (p <0.05). Conclusion: This study show that every patient diagnosed with vitiligo, independent of the IS, should undergo examination for autoimmune disease. A mild positive correlation between VTA and NLO-TLO was found to be an indicator of increased inflammation in vitiligo patients as the extent of lesions increased.

Prevalence of ADHD in children born by mothers consuming Frankincense during pregnancy

2021 ◽  
Author(s):  
Alimohammad Ranjbar ◽  
Elahe Kamali Ardakani ◽  
Rahele Zareshahi
Keyword(s):  
Data Gathering ◽  
Control Group ◽  
Case Group ◽  
Dsm V ◽  
History Of ◽  
The Difference ◽  
The Impact ◽  
The Relationship ◽  
The Brain ◽  
Positive Effect

Aims: In Iranian culture, due to some narratives from the prophet Mohammad about the use of frankincense during pregnancy for increasing IQ in children, some women consume frankincense during expectancy. This study's goal is to evaluate the relationship between frankincense used during pregnancy and the incidence of ADHD. Methods: In this study, the case group comprised children 4-17 years old referring to Shahid Chamran Pharmacy in Yazd from summer to winter 2018 for receiving Methylphenidate, those with whom a psychologist had identified ADHD based on DSM-V factors.  The control group included children of the same age group but without ADHD. For data gathering, a checklist was used with some questions on smoking, family history of ADHD, presence/absence of a specific disease during pregnancy, frankincense used during pregnancy, and a chemical medication consumed during pregnancy. Results: The main result demonstrated that the children whose mothers used frankincense during pregnancy were 0.67 times less likely to be affected by ADHD than those whose mothers did not use this substance. However, the difference failed to be statistically significant (P>0.05). Conclusion: Some studies report that frankincense can bear a positive effect on the development of the brain and possibly adequate formation of dendrites trees, axons and induce proper communication between them, so the impact of frankincense on the brain may be justified by its protective effect against the hyperactive child.

The Relationship of Drug Abuse to Unexplained Sudden Death

2008 ◽  
Vol 132 (12) ◽  
pp. 1903-1906
Author(s):  
Amy C. Gruszecki ◽  
Gerald McGwin, Jr ◽  
C. Andrew Robinson, Jr ◽  
Gregory G. Davis
Keyword(s):  
Drug Abuse ◽  
Cause Of Death ◽  
Control Group ◽  
Manner Of Death ◽  
Increased Risk ◽  
History Of ◽  
Matched Case ◽  
Relationship Of ◽  
The Relationship ◽  
Control Study

Abstract Context.—Forensic pathologists regularly investigate the deaths of individuals with a history of drug abuse. Autopsy, including toxicology testing, reveals no cause for death in a subset of this cohort. Objective.—To determine whether deaths with an undetermined cause and manner of death are associated with a history of drug abuse. Design.—Retrospective matched case-control study of 52 decedents whose cause of death remained undetermined following autopsy, matched 1:2 to a control group of living patients admitted for cholecystectomy according to age and date of death or procedure. Results.—Individuals whose cause of death was undetermined were 5.3 times (95% confidence interval, 1.9– 14.5) more likely to have a history of drug abuse than were patients with cholecystitis. Conclusions.—Decedents with a history of chronic drug abuse appear to be at an increased risk of dying by their chronic drug abuse, even in the absence of any anatomical or toxicologic finding at autopsy to account for death.

In search for polymorphic DNA markers to increase resistance and production level of hens

2020 ◽  
Vol 1 (12) ◽  
pp. 98-104
Author(s):  
I. I. Kochish ◽  
◽  
О. V. Myasnikova ◽  
V. V. Martynov ◽  
V. I. Smolensky ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Dopamine Receptor ◽  
Dna Markers ◽  
Production Level ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Productive Traits ◽  
Pure Lines ◽  
The Relationship

In the article, the authors assess the prospect of using genetic polymorphisms to predict resistance to diseases and a possible enlarge in chicken production level. Using the PCR-RFLP method, we analyzed the SNP frequencies in the genes of myostatin (MSTN, or GDF-8), proopiomelanocortin (POMC), and dopamine receptor D2 (DRD2) in chickens of the pure lines of the Smena-8 broiler hybreed – B6, B7m / o (slow-feathering), B7b / o (fast-fledging) FSBI "Breeding and Genetic Center “Smena”. The relationship between the studied single nucleotide polymorphisms and the productive traits of chickens was determined. The assessment of the possibilities of using polymorphisms to increase the resistance and productivity of linear birds.

scholarly journals Association of rs780094 polymorphism of glucokinase regulatory protein with non-alcoholic fatty liver disease

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Saba Mohammadi ◽  
Safar Farajnia ◽  
Masoud Shadmand ◽  
Fatemeh Mohseni ◽  
Roghayyeh Baghban
Keyword(s):  
Regulatory Protein ◽  
Single Nucleotide ◽  
Alcoholic Fatty Liver ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Study Population ◽  
Northwest Of Iran ◽  
And Control ◽  
The Relationship ◽  
Tabriz City

Abstract Objective GCK rs780094 polymorphism is a single nucleotide polymorphism that has been associated with obesity, type II diabetes and dyslipidemia in some populations, conditions that highly related to NAFL etiology. The present study aimed to evaluate the relationship between NAFLD and rs780094 polymorphism in patients with NAFLD in Tabriz city, northwest of Iran. The rs780094 polymorphism was determined in 74 patients with NAFLD by PCR–RFLP technique. Demographic information was collected using a questionnaire and biochemical analysis was performed using standard laboratory methods. Results There was a significant difference between case and control subjects for alanine aminotransferase, aspartate aminotransferase, HDL-C and triglycerides (P < 0.05). Analysis by PCR–RFLP method revealed that there were no significant differences between NAFLD and healthy subjects for rs780094 polymorphism in the study population. The results of this study indicated that rs780094 polymorphism is not associated with NAFLD in subjects from Tabriz city.

scholarly journals Association of single nucleotide polymorphisms in CACNA 1A/CACNA 1C/CACNA 1H calcium channel genes with diabetic peripheral neuropathy in Chinese population

2018 ◽  
Vol 38 (3) ◽  
Author(s):  
Lin Sun ◽  
Jun Ma ◽  
Qian Mao ◽  
Yun-Long Yang ◽  
Lin-Lin Ma ◽  
...  
Keyword(s):  
Peripheral Neuropathy ◽  
Single Nucleotide Polymorphisms ◽  
Calcium Channel ◽  
Diabetic Peripheral Neuropathy ◽  
Chinese Population ◽  
Control Group ◽  
Case Group ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Pcr Rflp

The present study was conducted to explore the correlations between single nucleotide polymorphisms (SNPs) in the calcium channel CACNA 1A, CACNA 1C, and CACNA 1H genes and diabetic peripheral neuropathy (DPN) amongst the Chinese population. In total, 281 patients diagnosed with type 2 diabetes participated in the present study. These patients were divided into the case group, which was subdivided into the DPN (143 cases) and the non-DPN groups (138 cases). Subsequently, 180 healthy individuals that had undergone routine health examinations were also recruited and assigned to the control group. PCR-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotype and allele frequencies of CACNA 1A, CACNA 1C, and CACNA 1H genes; logistic regression analysis to investigate the association of gene polymorphisms with DNP. Gene–gene interactions were then detected by generalized multifactor dimensionality reduction (GMDR). The results revealed that CACNA 1A rs2248069 and rsl6030, CACNA 1C rs216008 and rs2239050, and CACNA 1H rs3794619, and rs7191246 SNPs were all associated with DPN, while rs2248069, rsl6030, rs2239050, and rs7191246 polymorphisms were attributed to the susceptibility to DPN. It was also observed that the optimal models were three-, four- and five-dimensional models with a prediction accuracy of 61.05% and the greatest consistency of cross-validation was 10/10. In summary, these findings demonstrated that the SNPs in the CACNA 1A, CACNA 1C, and CACNA 1H genes were involved in the pathophysiology of DPN. In addition, polymorphisms in the CACNA 1A, CACNA 1C, and CACNA 1H genes and their interactions also had effects on DPN.

scholarly journals Single Nucleotide Polymorphisms inP2X7Gene Are Associated with Serum Immunoglobulin G Responses toMycobacterium tuberculosisin Tuberculosis Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Jiangdong Wu ◽  
Lijun Lu ◽  
Le Zhang ◽  
Yulei Ding ◽  
Fang Wu ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Control Group ◽  
Case Group ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Mutant Genotype ◽  
Pcr Rflp ◽  
Positive Rate

Objective. Our study investigated the association between single nucleotide polymorphisms (SNPs) in P2X7 gene and serum immunoglobulin G (IgG) responses to mycobacterium tuberculosis (MTB) in TB patients.Methods. A total of 103 TB patients were enrolled as case group and 87 healthy individuals at same geographical region as control group. The SNP detection of 1513A>C and -762T>C was performed using PCR-RFLP, and the levels of serum IgG responses to MTB in all subjects were determined.Results. AC and CC of 1513A>C and TC and CC of -762T>C had higher frequencies in case group than in control group. TB patients carrying TC and CC of -762T>C had higher positive rate of IgG responses to MTB than those carrying TT. Additionally, patients carrying TC and CC of -762T>C had more MTB in sputum than those carrying TT.Conclusion. P2X7 SNPs, 1513A>C and -762T>C, may be associated with the susceptibility to tuberculosis, and -762T>C SNP may contribute to the development of MTB. The mutant genotype of -762T>C (TC and CC) may lower human capability of phagocytosis to MTB, leading to an increased morbidity of TB.

scholarly journals Comparison of Shoplifting Behaviours in Patients with Eating Disorders, Psychiatric Control Subjects, and Undergraduate Control Subjects

2000 ◽  
Vol 45 (5) ◽  
pp. 471-475 ◽  
Author(s):  
Elliot M Goldner ◽  
Josie Geller ◽  
C Laird Birmingham ◽  
Ronald A Remick
Keyword(s):  
Eating Disorders ◽  
Eating Disorder ◽  
Self Esteem ◽  
Control Group ◽  
Control Subjects ◽  
The Past ◽  
History Of ◽  
Eating Disorder Symptomatology ◽  
The Relationship

Shoplifting behaviours were examined in an eating disorder group (EDG, n = 48), a psychiatric control group (PCG, n = 46), and an undergraduate control group (UCG, n = 82). They were examined in relation to self-esteem, depression, and eating disorder symptomatology. The 3 groups did not differ in overall history of shoplifting, but EDG women were more likely to have shoplifted in the past 6 months (current shoplifting) and to have shoplifted often than were women from the PCG or UCG. Across all 3 groups, current shoplifting was associated with low self-esteem, elevated depression, and purging behaviours at the time of the assessment. The implications of these findings with regard to the relationship between shoplifting and eating disorder symptomatology will be addressed.

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