Human dental pulp stem cells expressing transforming growth factor β3 transgene for cartilage-like tissue engineering

Cytotherapy ◽  
2013 ◽  
Vol 15 (6) ◽  
pp. 712-725 ◽  
Author(s):  
Ahmed Rizk ◽  
A. Bakr M. Rabie
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Growth Factor ◽  
Dental Pulp ◽  
Transforming Growth Factor ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Bing Song ◽  
Wenkai Jiang ◽  
Amr Alraies ◽  
Qian Liu ◽  
Vijay Gudla ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Dental Pulp ◽  
Transforming Growth Factor ◽  
Dental Pulp Stem Cells ◽  
Specific Marker ◽  
Bladder Tissue ◽  
Growth Environment ◽  
Gene And Protein Expression

Dental pulp stem cells (DPSCs) are multipotent cells capable of differentiating into multiple cell lines, thus providing an alternative source of cell for tissue engineering. Smooth muscle cell (SMC) regeneration is a crucial step in tissue engineering of the urinary bladder. It is known that DPSCs have the potential to differentiate into a smooth muscle phenotype in vitro with differentiation agents. However, most of these studies are focused on the vascular SMCs. The optimal approaches to induce human DPSCs to differentiate into bladder SMCs are still under investigation. We demonstrate in this study the ability of human DPSCs to differentiate into bladder SMCs in a growth environment containing bladder SMCs-conditioned medium with the addition of the transforming growth factor beta 1 (TGF-β1). After 14 days of exposure to this medium, the gene and protein expression of SMC-specific marker (α-SMA, desmin, and calponin) increased over time. In particular, myosin was present in differentiated cells after 11 days of induction, which indicated that the cells differentiated into the mature SMCs. These data suggested that human DPSCs could be used as an alternative and less invasive source of stem cells for smooth muscle regeneration, a technology that has applications for bladder tissue engineering.

scholarly journals The Effects of Platelet-Derived Growth Factor-BB on Human Dental Pulp Stem Cells Mediated Dentin-Pulp Complex Regeneration

2017 ◽  
Vol 6 (12) ◽  
pp. 2126-2134 ◽  
Author(s):  
Maolin Zhang ◽  
Fei Jiang ◽  
Xiaochen Zhang ◽  
Shaoyi Wang ◽  
Yuqin Jin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Platelet Derived Growth Factor ◽  
Human Dental Pulp

Average cell viability levels of human dental pulp stem cells: an accurate combinatorial index for quality control in tissue engineering

Cytotherapy ◽  
2013 ◽  
Vol 15 (4) ◽  
pp. 507-518 ◽  
Author(s):  
Miguel Angel Martin-Piedra ◽  
Ingrid Garzon ◽  
Ana Celeste Oliveira ◽  
Camilo Andres Alfonso-Rodriguez ◽  
Maria Carmen Sanchez-Quevedo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Quality Control ◽  
Cell Viability ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Average Cell ◽  
Human Dental Pulp
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