Comparative analysis of the influence of cryoprotective agents and a long-term storage on human Adipose tissue-Mesenchimal Stromal Cells

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S100
Author(s):  
O. Espinosa Ibáñez ◽  
A. Fernández-González ◽  
Á. Sierra-Sánchez ◽  
J. Guerrero ◽  
N. Fernández-Porcel ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Comparative Analysis ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Is adipose tissue suitable for detection of (synthetic) cannabinoids? A comparative study analyzing antemortem and postmortem specimens following pulmonary administration of JWH-210, RCS-4, as well as ∆9-tetrahydrocannabinol to pigs

2020 ◽  
Vol 94 (10) ◽  
pp. 3421-3431
Author(s):  
Nadine Schaefer ◽  
Frederike Nordmeier ◽  
Ann-Katrin Kröll ◽  
Christina Körbel ◽  
Matthias W. Laschke ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Synthetic Cannabinoids ◽  
Standard Addition ◽  
Storage Conditions ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Concentration Changes ◽  
Term Storage ◽  
Plateau Level

Abstract Examining fatal poisonings, chronic exposure may be reflected by the concentration in tissues known for long-term storage of drugs. Δ9-tetrahydrocannabinol (THC) persists in adipose tissue (AT), but sparse data on synthetic cannabinoids (SC) are available. Thus, a controlled pig study evaluating antemortem (AM) disposition and postmortem (PM) concentration changes of the SC 4-ethylnaphthalene-1-yl-(1-pentylindole-3-yl)methanone (JWH-210) and 2-(4-methoxyphenyl)-1-(1-pentyl-indole-3-yl)methanone (RCS-4) as well as THC in AT was performed. The drugs were administered pulmonarily (200 µg/kg body weight) to twelve pigs. Subcutaneous (s.c.) AT specimens were collected after 15 and 30 min and then hourly up to 8 h. At the end, pigs were sacrificed and s.c., perirenal, and dorsal AT specimens were collected. The carcasses were stored at room temperature (RT; n = 6) or 4 °C (n = 6) and specimens were collected after 24, 48, and 72 h. After homogenization in acetonitrile and standard addition, LC–MS/MS was performed. Maximum concentrations were reached 0.5–2 h after administration amounting to 21 ± 13 ng/g (JWH-210), 24 ± 13 ng/g (RCS-4), and 22 ± 20 ng/g (THC) and stayed at a plateau level. Regarding the metabolites, very low concentrations of N-hydroxypentyl-RCS-4 (HO-RCS-4) were detected from 0.5 to 8 h. PM concentrations of parent compounds did not change significantly (p > 0.05) over time under both storage conditions. Concentrations of HO-RCS-4 significantly (p < 0.05) increased in perirenal AT during storage at RT. These results suggest a rapid distribution and persistence in s.c. AT. Furthermore, AT might be resistant to PM redistribution of parent compounds. However, significant PM increases of metabolite concentrations might be considered in perirenal AT.

scholarly journals Preservation of Avian Cells at Sub-zero Temperatures

1979 ◽  
Vol 32 (5) ◽  
pp. 475 ◽  
Author(s):  
N Ratnamohan ◽  
PB Spradbrow
Keyword(s):  
Dimethyl Sulfoxide ◽  
Cryoprotective Agent ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Short And Long Term ◽  
Term Storage

The cryoprotective agents dimethyl sulfoxide (DMSO), glycerol, polyvinylpyrrolidone (PVP) and dextran were evaluated for their ability to protect avian cells during storage at sub-zero temperatures. DMSO was the most effective cryoprotective agent for the short- and long-term storage of avian cells and glycerol was also effective when used at low concentrations. PVP and dextran did not protect avian cells during storage in our experiments. Primary chicken cells and avian cells at higher passage levels were successfully recovered after storage with DMSO for periods ranging from 4 to 12 months.

scholarly journals Long-Term Storage of Fish Oil Fatty Acids in the Liver, Not Adipose Tissue, in Humans.

1992 ◽  
Vol 12 (1) ◽  
pp. 51-57
Author(s):  
Yoshisuke MARUHAMA ◽  
Yoshihito KANEKO ◽  
Kohsaku SEKINE ◽  
Tuguhisa KURODA ◽  
Hideaki MUKAIDA ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fish Oil ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Novel Approaches to the Cryopreservation of Human Spermatozoa: History and Development of the Spermatozoa Vitrification Technology

2011 ◽  
Vol 2 (2) ◽  
pp. 128-145 ◽  
Author(s):  
Evgenia Isachenko ◽  
Gohar Rahimi ◽  
Peter Mallmann ◽  
Raul Sanchez ◽  
Vladimir Isachenko
Keyword(s):  
Ice Crystals ◽  
Intracellular Lipids ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Cryopreservation Techniques ◽  
Water Quality And Quantity ◽  
And Storage ◽  
Term Storage ◽  
Conventional Freezing

Cryobiology is very intensively applied in reproductive and veterinary medicine for preservation of gametes, embryos and reproductive tissues. Sub-zero temperatures combined with appropriate cryoprotective agents preserve the physiological and reproductive functions of the cells making long-term storage possible without loss of viability. With the use of cryoprotective agents it has become possible to develop cryopreservation techniques, such as the slow conventional freezing and vitrification that are in use in the present times. In slow controlled-rate conventional freezing extracellular ice crystals are formed whereas in vitrification no ice crystals are formed. Glass formation is compatible with the survival of the cell and the preservation of its intracellular structures provided the type(s) and concentrations of cryoprotectant used are not chemo- or osmotoxic. However, irrespective of the type of cooling method employed the cryosurvival of cells and tissues is influenced by the size and maturity of cells, amounts of intracellular water, quality and quantity of intracellular lipids, type of cells, their function and morphology. The intracellular milieu of cryopreserved cells and tissues remain less understood. The application of nanotechnology may help reveal and help advance our knowledge of the cryobiological principles involved in cryosurvival. At this moment the methods of cryopreservation that merit further investigation are vitrification and lyophilization. Vitrification is cheap if reagents are prepared in-house and the procedure can be performed rapidly. It has been successfully applied for gametes and embryos (of different stages of development), and reproductive cells/tissues, somatic cells and stem cells. However, vitrification is more demanding technically and requires operation and storage at sub-zero temperatures. On the other hand lyophilization deserves further investigation because it is a cheaper form of cryopreservation that may enable cryostorage at less demanding temperatures of 4°C and may even allow transport at ambient temperature. These possibilities are explored in this review.

scholarly journals Carboxylated Poly-l-Lysine as a Macromolecular Cryoprotective Agent Enables the Development of Defined and Xeno-Free Human Sperm Cryopreservation Reagents

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1435
Author(s):  
Hiroki Takeuchi ◽  
Mikiko Nishioka ◽  
Tadashi Maezawa ◽  
Yuko Kitano ◽  
Kento Terada-Yoshikawa ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Human Sperm ◽  
Allergic Reactions ◽  
Sperm Cryopreservation ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Term Storage

In human sperm cryopreservation, test yolk buffer and human serum albumin have been used as permeating macromolecular-weight cryoprotectants. In clinical reproductive medicine, human serum albumin is frequently used because of low risks of zoonoses and allergic reactions. However, the risk of allogeneic infectious diseases exists, and the supply may be unstable because human serum albumin is derived from human blood. Therefore, the development of xeno-free human sperm cryopreservative reagents that could overcome the aforementioned problems is warranted. We succeeded in developing a new xeno-free and defined sperm cryopreservation reagent containing glycerol, carboxylated poly-l-lysine, and raffinose. The cryopreservation reagent was not significantly different in terms of sperm motility, viability, and DNA fragmentation and was comparable in performance to a commercial cryopreservation reagent containing human serum albumin. Moreover, the addition of saccharides was essential for its long-term storage. These results may help elucidate the unknown function of macromolecular-weight permeating cryoprotective agents.

scholarly journals An off-the-shelf artificial cardiac patch improves cardiac repair after myocardial infarction in rats and pigs

2020 ◽  
Vol 12 (538) ◽  
pp. eaat9683 ◽  
Author(s):  
Ke Huang ◽  
Emily W. Ozpinar ◽  
Teng Su ◽  
Junnan Tang ◽  
Deliang Shen ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stromal Cells ◽  
Cardiac Repair ◽  
Myocardial Repair ◽  
Long Term Storage ◽  
Secreted Factors ◽  
Cardiac Patch ◽  
Extracellular Matrix Scaffold ◽  
Term Storage

Cell therapy has been a promising strategy for cardiac repair after injury or infarction; however, low retention and engraftment of transplanted cells limit potential therapeutic efficacy. Seeding scaffold material with cells to create cardiac patches that are transplanted onto the surface of the heart can overcome these limitations. However, because patches need to be freshly prepared to maintain cell viability, long-term storage is not feasible and limits clinical applicability. Here, we developed an off-the-shelf therapeutic cardiac patch composed of a decellularized porcine myocardial extracellular matrix scaffold and synthetic cardiac stromal cells (synCSCs) generated by encapsulating secreted factors from isolated human cardiac stromal cells. This fully acellular artificial cardiac patch (artCP) maintained its potency after long-term cryopreservation. In a rat model of acute myocardial infarction, transplantation of the artCP supported cardiac recovery by reducing scarring, promoting angiomyogenesis, and boosting cardiac function. The safety and efficacy of the artCP were further confirmed in a porcine model of myocardial infarction. The artCP is a clinically feasible, easy-to-store, and cell-free alternative to myocardial repair using cell-based cardiac patches.

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