scholarly journals Carboxylated Poly-l-Lysine as a Macromolecular Cryoprotective Agent Enables the Development of Defined and Xeno-Free Human Sperm Cryopreservation Reagents

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1435
Author(s):  
Hiroki Takeuchi ◽  
Mikiko Nishioka ◽  
Tadashi Maezawa ◽  
Yuko Kitano ◽  
Kento Terada-Yoshikawa ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Human Sperm ◽  
Allergic Reactions ◽  
Sperm Cryopreservation ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Term Storage

In human sperm cryopreservation, test yolk buffer and human serum albumin have been used as permeating macromolecular-weight cryoprotectants. In clinical reproductive medicine, human serum albumin is frequently used because of low risks of zoonoses and allergic reactions. However, the risk of allogeneic infectious diseases exists, and the supply may be unstable because human serum albumin is derived from human blood. Therefore, the development of xeno-free human sperm cryopreservative reagents that could overcome the aforementioned problems is warranted. We succeeded in developing a new xeno-free and defined sperm cryopreservation reagent containing glycerol, carboxylated poly-l-lysine, and raffinose. The cryopreservation reagent was not significantly different in terms of sperm motility, viability, and DNA fragmentation and was comparable in performance to a commercial cryopreservation reagent containing human serum albumin. Moreover, the addition of saccharides was essential for its long-term storage. These results may help elucidate the unknown function of macromolecular-weight permeating cryoprotective agents.

scholarly journals EFFECTS OF LONG-TERM STORAGE ON HUMAN SERUM ALBUMIN. I. CHROMATOGRAPHIC AND ULTRACENTRIFUGAL ASPECTS

1960 ◽  
Vol 39 (12) ◽  
pp. 1837-1840 ◽  
Author(s):  
J. S. Finlayson ◽  
Richard T. Suchinsky ◽  
Ann L. Dayton
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Long Term Storage ◽  
Term Storage

Long-term release of chlorhexidine from dental adhesive resin system using human serum albumin nanoparticles

2014 ◽  
Vol 71 (4) ◽  
pp. 875-886 ◽  
Author(s):  
Hyun-Jin Kim ◽  
Tae-Yub Kwon ◽  
Kyo-Han Kim ◽  
Soon-Taek Kwon ◽  
Dae-Hyun Cho ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Resin System ◽  
Adhesive Resin ◽  
Dental Adhesive ◽  
Albumin Nanoparticles

scholarly journals Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Metabolites ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 93 ◽  
Author(s):  
Cora McHugh ◽  
Thomas Flott ◽  
Casey Schooff ◽  
Zyad Smiley ◽  
Michael Puskarich ◽  
...  
Keyword(s):  
Human Serum ◽  
1H Nmr ◽  
Whole Blood ◽  
Nmr Spectra ◽  
High Quality ◽  
Healthy Human ◽  
Long Term Storage ◽  
The Impact ◽  
Term Storage

Background: Though blood is an excellent biofluid for metabolomics, proteins and lipids present in blood can interfere with 1d-1H NMR spectra and disrupt quantification of metabolites. Here, we present effective macromolecule removal strategies for serum and whole blood (WB) samples. Methods: A variety of macromolecule removal strategies were compared in both WB and serum, along with tests of ultrafiltration alone and in combination with precipitation methods. Results: In healthy human serum, methanol:chloroform:water extraction with ultrafiltration was compared to methanol precipitation with and without ultrafiltration. Methods were tested in healthy pooled human serum, and in serum from patients with sepsis. Effects of long-term storage at −80 °C were tested to explore the impact of macromolecule removal strategy on serum from different conditions. In WB a variety of extraction strategies were tested in two types of WB (from pigs and baboons) to examine the impact of macromolecule removal strategies on different samples. Conclusions: In healthy human serum methanol precipitation of serum with ultrafiltration was superior, but was similar in recovery and variance to methanol:chloroform:water extraction with ultrafiltration in pooled serum from patients with sepsis. In WB, high quality, quantifiable spectra were obtained with the use of a methanol: chloroform precipitation.

Comparative analysis of the influence of cryoprotective agents and a long-term storage on human Adipose tissue-Mesenchimal Stromal Cells

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S100
Author(s):  
O. Espinosa Ibáñez ◽  
A. Fernández-González ◽  
Á. Sierra-Sánchez ◽  
J. Guerrero ◽  
N. Fernández-Porcel ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Comparative Analysis ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Term Storage

Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration

2000 ◽  
Vol 38 (6) ◽  
Author(s):  
Françoise Schiele ◽  
Monique Vincent-Viry ◽  
Bernard Herbeth ◽  
Athanase Visvikis ◽  
Gérard Siest
Keyword(s):  
Apolipoprotein E ◽  
Human Serum ◽  
Long Term Storage ◽  
Short And Long Term ◽  
Term Storage

scholarly journals Stability of Folate and Vitamin B12in Human Serum after Long-Term Storage: A Follow-Up after 13 Years

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
Eugène H. J. M. Jansen ◽  
Piet K. Beekhof
Keyword(s):  
Human Serum ◽  
Storage Time ◽  
Vitamin B ◽  
Serum Samples ◽  
Long Term Storage ◽  
Nutrition Research ◽  
The Stability ◽  
Term Storage

In epidemiological and nutrition research, it is very important to evaluate the stability of biomarkers as function of both storage time and temperature. In this study, the stability of folate and vitamin B12in human serum samples has been tested after long-term storage at −80°C up to 13 years. Serum samples of 16 individuals were used in this study. The concentration of folate and vitamin B12has been determined att=0and at 1, 8, and 13 years after storage at −80°C. The folate concentrations in serum samples remained stable at −80°C. The concentration of vitamin B12was decreasing during the time of the study to about 50%. The correlation of the folate and also of the vitamin B12concentrations in the stored samples compared with the starting values was still good. Therefore, although the concentration of vitamin B12decreased upon storage, reliable comparative analyses can still be performed.

scholarly journals Preservation of Avian Cells at Sub-zero Temperatures

1979 ◽  
Vol 32 (5) ◽  
pp. 475 ◽  
Author(s):  
N Ratnamohan ◽  
PB Spradbrow
Keyword(s):  
Dimethyl Sulfoxide ◽  
Cryoprotective Agent ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Short And Long Term ◽  
Term Storage

The cryoprotective agents dimethyl sulfoxide (DMSO), glycerol, polyvinylpyrrolidone (PVP) and dextran were evaluated for their ability to protect avian cells during storage at sub-zero temperatures. DMSO was the most effective cryoprotective agent for the short- and long-term storage of avian cells and glycerol was also effective when used at low concentrations. PVP and dextran did not protect avian cells during storage in our experiments. Primary chicken cells and avian cells at higher passage levels were successfully recovered after storage with DMSO for periods ranging from 4 to 12 months.

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