Label-free immunosensor based on Au@Ag2S nanoparticles/magnetic chitosan matrix for sensitive determination of ractopamine

2015 ◽  
Vol 741 ◽  
pp. 14-19 ◽  
Author(s):  
Yong Zhang ◽  
Hongmin Ma ◽  
Dan Wu ◽  
Yan Li ◽  
Bin Du ◽  
...  
Keyword(s):  
Label Free ◽  
Magnetic Chitosan ◽  
Chitosan Matrix ◽  
Sensitive Determination ◽  
Ag2s Nanoparticles

scholarly journals Label-Free and Sensitive Determination of Cadmium Ions Using a Ti-Modified Co3O4-Based Electrochemical Aptasensor

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 195
Author(s):  
Yang Liu ◽  
Dongwei Zhang ◽  
Jina Ding ◽  
Kashif Hayat ◽  
Xijia Yang ◽  
...  
Keyword(s):  
Environmental Protection Agency ◽  
Concentration Limit ◽  
Co3o4 Nanoparticles ◽  
Label Free ◽  
Current Signal ◽  
Electrochemical Aptasensor ◽  
Screen Printed Carbon Electrode ◽  
Sensitive Determination

The current work demonstrates an electrochemical aptasensor for sensitive determination of Cd2+ based on the Ti-modified Co3O4 nanoparticles. In this unlabeled system, Ti-modified Co3O4 nanoparticles act as current signal amplifiers modified on the screen-printed carbon electrode (SPCE) surface, while the derivative aptamer of Cd2+ works as a target recognizer. In addition, the sensing is based on the increase in electrochemical probe thionine current signal due to the binding of aptamer to Cd2+ via specific recognition. In the current study, key parameters, including aptamer concentration, pH, and incubation time were optimized, respectively, to ensure sensing performance. Cyclic voltammetry was used not only to characterize each preparation and optimization step, but also to profile the bindings of aptamer to Cd2+. Under optimal conditions, Cd2+ can be determined in a linear range of 0.20 to 15 ng/mL, with a detection limit of 0.49 ng/mL, significantly below the maximum concentration limit set by the U.S. Environmental Protection Agency. Based on comparative analysis and the results of recovery test with real samples, this simple, label-free but highly selective method has considerable potential and thus can be used as an in-situ environmental monitoring platform for Cd2+ testing.

scholarly journals Electrochemical detection of serotonin based on a poly(bromocresol green) film and Fe3O4 nanoparticles in a chitosan matrix

RSC Advances ◽  
2017 ◽  
Vol 7 (4) ◽  
pp. 1847-1851 ◽  
Author(s):  
Gu Ran ◽  
Xing Chen ◽  
Ying Xia
Keyword(s):  
Carbon Nanotubes ◽  
Magnetic Nanoparticles ◽  
Composite Film ◽  
Electrochemical Detection ◽  
Fe3o4 Nanoparticles ◽  
Bromocresol Green ◽  
Multiwalled Carbon ◽  
Chitosan Matrix ◽  
Sensitive Determination

A composite film containing poly(bromocresol green), magnetic nanoparticles and multiwalled carbon nanotubes was fabricated for the sensitive determination of serotonin.

Novel label-free electrochemical strategy for sensitive determination of ten-eleven translocation protein 1

2020 ◽  
Author(s):  
Zhenya Yu ◽  
Xue Chen ◽  
Ying Cheng ◽  
Hongmei Yang ◽  
Fang Wang ◽  
...  
Keyword(s):  
Label Free ◽  
Sensitive Determination

scholarly journals A White Light Reflectance Spectroscopy Label-Free Biosensor for the Determination of Fungicide Carbendazim

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 45
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Sotirios Kakabakos ◽  
Evangelia Livaniou
Keyword(s):  
White Light ◽  
Reflectance Spectroscopy ◽  
Food Samples ◽  
Environmental Pollutant ◽  
Label Free ◽  
Reliable Determination ◽  
Light Reflectance ◽  
Sensitive Determination

Carbendazim is a broad-spectrum benzimidazole-type fungicide effective against fungi that compromise the safety/quality of food products. Despite its potential usefulness, carbendazim constitutes a major environmental pollutant, being hazardous for humans and animals; therefore, reliable determination of carbendazim levels in water, soil, and food samples remains a highly desirable analytical goal. Herein, an optical (white light reflectance spectroscopy, WLRS) label-free biosensor for fast and sensitive determination of carbendazim is presented. The transducer is a SiO2/Si chip, on which a suitable benzimidazole-conjugate has been immobilized; determination is based on the competitive immunoassay format: A mixture of an in-house developed anti-carbendazim antibody with the calibrators/samples is pumped over the chip, followed by biotinylated secondary antibody and unlabeled streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the SiO2/Si chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is capable of detecting carbendazim levels within 28 min (LoD: 20 ng/mL; intra- and inter-assay CVs: ≤6.9% and ≤9.4%, respectively). Excellent analytical characteristics and short analysis time combined with its small size render the proposed WLRS biosensor ideal for future point-of-need determination of carbendazim in food and environmental samples.

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