scholarly journals A White Light Reflectance Spectroscopy Label-Free Biosensor for the Determination of Fungicide Carbendazim

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 45
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Sotirios Kakabakos ◽  
Evangelia Livaniou
Keyword(s):  
White Light ◽  
Reflectance Spectroscopy ◽  
Food Samples ◽  
Environmental Pollutant ◽  
Label Free ◽  
Reliable Determination ◽  
Light Reflectance ◽  
Sensitive Determination

Carbendazim is a broad-spectrum benzimidazole-type fungicide effective against fungi that compromise the safety/quality of food products. Despite its potential usefulness, carbendazim constitutes a major environmental pollutant, being hazardous for humans and animals; therefore, reliable determination of carbendazim levels in water, soil, and food samples remains a highly desirable analytical goal. Herein, an optical (white light reflectance spectroscopy, WLRS) label-free biosensor for fast and sensitive determination of carbendazim is presented. The transducer is a SiO2/Si chip, on which a suitable benzimidazole-conjugate has been immobilized; determination is based on the competitive immunoassay format: A mixture of an in-house developed anti-carbendazim antibody with the calibrators/samples is pumped over the chip, followed by biotinylated secondary antibody and unlabeled streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the SiO2/Si chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is capable of detecting carbendazim levels within 28 min (LoD: 20 ng/mL; intra- and inter-assay CVs: ≤6.9% and ≤9.4%, respectively). Excellent analytical characteristics and short analysis time combined with its small size render the proposed WLRS biosensor ideal for future point-of-need determination of carbendazim in food and environmental samples.

scholarly journals Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 153
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Ioannis Raptis ◽  
Panagiota Petrou ◽  
Evangelia Livaniou ◽  
...  
Keyword(s):  
White Light ◽  
Optical Biosensor ◽  
Reflectance Spectroscopy ◽  
Fruit Juices ◽  
Sample Treatment ◽  
Assay Sensitivity ◽  
Reliable Determination ◽  
Light Reflectance ◽  
Sensitive Determination

Carbendazim is a systemic benzimidazole-type fungicide with broad-spectrum activity against fungi that undermine food products safety and quality. Despite its effectiveness, carbendazim constitutes a major environmental pollutant, being hazardous to both humans and animals. Therefore, fast and reliable determination of carbendazim levels in water, soil, and food samples is of high importance for both food industry and public health. Herein, an optical biosensor based on white light reflectance spectroscopy (WLRS) for fast and sensitive determination of carbendazim in fruit juices is presented. The transducer is a Si/SiO2 chip functionalized with a benzimidazole conjugate, and determination is based on a competitive immunoassay format. Thus, for the assay, a mixture of an in-house developed rabbit polyclonal anti-carbendazim antibody with the standards or samples is pumped over the chip, followed by biotinylated secondary antibody and streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the Si/SiO2 chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is able to detect 20 ng/mL of carbendazim in fruit juices with high accuracy and precision (intra- and inter-assay CVs ≤ 6.9% and ≤9.4%, respectively) in less than 30 min, applying a simple sample treatment that alleviates any “matrix-effect” on the assay results and a 60 min preincubation step for improving assay sensitivity. Excellent analytical characteristics and short analysis time along with its small size render the proposed WLRS immunosensor ideal for future on-the-spot determination of carbendazim in food and environmental samples.

Rapid and sensitive label-free determination of aflatoxin M1 levels in milk through a White Light Reflectance Spectroscopy immunosensor

2019 ◽  
Vol 282 ◽  
pp. 104-111 ◽  
Author(s):  
Dimitra Tsounidi ◽  
Georgios Koukouvinos ◽  
Panagiota Petrou ◽  
Konstantinos Misiakos ◽  
Grigorios Zisis ◽  
...  
Keyword(s):  
White Light ◽  
Reflectance Spectroscopy ◽  
Aflatoxin M1 ◽  
Label Free ◽  
Light Reflectance

A label-free flow-through immunosensor for determination of total- and free-PSA in human serum samples based on white-light reflectance spectroscopy

2015 ◽  
Vol 209 ◽  
pp. 1041-1048 ◽  
Author(s):  
G. Koukouvinos ◽  
P.S. Petrou ◽  
K. Misiakos ◽  
D. Drygiannakis ◽  
I. Raptis ◽  
...  
Keyword(s):  
Human Serum ◽  
White Light ◽  
Reflectance Spectroscopy ◽  
Free Flow ◽  
Label Free ◽  
Serum Samples ◽  
Free Psa ◽  
Light Reflectance ◽  
Flow Through

scholarly journals Fast Deoxynivalenol Determination in Cereals Using a White Light Reflectance Spectroscopy Immunosensor

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 154
Author(s):  
Vasileios Anastasiadis ◽  
Ioannis Raptis ◽  
Anastasios Economou ◽  
Sotirios Kakabakos ◽  
Panagiota Petrou
Keyword(s):  
White Light ◽  
Fusarium Species ◽  
Reflectance Spectroscopy ◽  
Human Consumption ◽  
Label Free ◽  
Light Reflectance ◽  
Optical Immunosensor ◽  
Maize Grain ◽  
Maximum Allowable Concentrations

Deoxynivalenol (DON) is a mycotoxin produced by certain Fusarium species and found in a high percentage of wheat and maize grains cultured worldwide. Although not so toxic as other mycotoxins, it exhibits both chronic and acute toxicity, and therefore methods for its fast and accurate on-site determination are highly desirable. In the current work, we employ an optical immunosensor based on White Light Reflectance Spectroscopy (WLRS) for the fast and sensitive immunochemical label-free determination of DON in wheat and maize samples. The assay is completed in 12 min and has a quantification limit of 2.5 ng/mL in buffer corresponding to 125 μg/kg in whole grain which is lower than the maximum allowable concentrations set by the regulatory authorities for grains intended for human consumption. Several extraction protocols have been compared, and the highest recovery (>90%) was achieved employing distilled water. In addition, identical calibration curves were received in buffer and wheat/maize extraction matrix providing the ability to analyze the grain samples using calibrators in buffer. Recoveries of DON from spiked wheat and maize grain samples ranged from 92.0(±4.0) to 105(±4.0)%. The analytical performance of the WLRS immunosensor, combined with the short analysis time and instrument portability, supports its potential for on-site determinations.

Fast, sensitive and selective determination of herbicide glyphosate in water samples with a White Light Reflectance Spectroscopy immunosensor

Talanta ◽  
2020 ◽  
Vol 214 ◽  
pp. 120854 ◽  
Author(s):  
Eleftheria Stavra ◽  
Panagiota S. Petrou ◽  
Georgios Koukouvinos ◽  
Anastasios Economou ◽  
Dimitris Goustouridis ◽  
...  
Keyword(s):  
White Light ◽  
Water Samples ◽  
Reflectance Spectroscopy ◽  
Selective Determination ◽  
Light Reflectance ◽  
Herbicide Glyphosate

Real-time multi-analyte label-free detection of proteins by white light reflectance spectroscopy

2014 ◽  
Author(s):  
Panagiota Petrou ◽  
Georgios Koukouvinos ◽  
Dimitrios Drygiannakis ◽  
Dimitris Goustouridis ◽  
Ioannis Raptis ◽  
...  
Keyword(s):  
Real Time ◽  
White Light ◽  
Reflectance Spectroscopy ◽  
Label Free ◽  
Label Free Detection ◽  
Light Reflectance

Rapid C-reactive protein determination in whole blood with a White Light Reflectance Spectroscopy label-free immunosensor for Point-of-Care applications

2018 ◽  
Vol 260 ◽  
pp. 282-288 ◽  
Author(s):  
Georgios Koukouvinos ◽  
Dimitrios Goustouridis ◽  
Konstantinos Misiakos ◽  
Sotirios Kakabakos ◽  
Ioannis Raptis ◽  
...  
Keyword(s):  
White Light ◽  
Whole Blood ◽  
Point Of Care ◽  
Reflectance Spectroscopy ◽  
Label Free ◽  
C Reactive Protein ◽  
Protein Determination ◽  
Reactive Protein ◽  
Light Reflectance

scholarly journals Development and Bioanalytical Applications of a White Light Reflectance Spectroscopy Label-Free Sensing Platform

Biosensors ◽  
2017 ◽  
Vol 7 (4) ◽  
pp. 46 ◽  
Author(s):  
Georgios Koukouvinos ◽  
Panagiota Petrou ◽  
Dimitrios Goustouridis ◽  
Konstantinos Misiakos ◽  
Sotirios Kakabakos ◽  
...  
Keyword(s):  
White Light ◽  
Reflectance Spectroscopy ◽  
Label Free ◽  
Sensing Platform ◽  
Light Reflectance ◽  
Bioanalytical Applications

Label-free kinetic study of biomolecular interactions by white light reflectance spectroscopy

2006 ◽  
Vol 1 (2) ◽  
pp. 94 ◽  
Author(s):  
M. Zavali ◽  
P.S. Petrou ◽  
S.E. Kakabakos ◽  
M. Kitsara ◽  
I. Raptis ◽  
...  
Keyword(s):  
Kinetic Study ◽  
White Light ◽  
Reflectance Spectroscopy ◽  
Biomolecular Interactions ◽  
Label Free ◽  
Light Reflectance

Simultaneous determination of CRP and D-dimer in human blood plasma samples with White Light Reflectance Spectroscopy

2016 ◽  
Vol 84 ◽  
pp. 89-96 ◽  
Author(s):  
Georgios Koukouvinos ◽  
Panagiota Petrou ◽  
Konstantinos Misiakos ◽  
Dimitris Drygiannakis ◽  
Ioannis Raptis ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Simultaneous Determination ◽  
Human Blood ◽  
White Light ◽  
Reflectance Spectroscopy ◽  
Human Blood Plasma ◽  
Plasma Samples ◽  
Light Reflectance ◽  
D Dimer
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