Certain in vivo studies have shown that the application of adhesives directly
onto the open pulp or on a thin layer of dentin causes inflammation and
pulpal abscesses. This reaction is related to toxic effects of monomers from
adhesives. It has been confirmed that after proper illumination the adhesives
become less toxic. The aim of the study was to examine genotoxicity of
non-polymerised, partly polymerised and polymerised adhesives on isolated
human lymphocytes using the alkaline Comet assay. Adper Single bond2 and
Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar
Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations
of 100, 200, 500 and 1000 ?g/mL. With both adhesives, photopolymerisation at
0 and 10 seconds showed statistically significant increase in DNA damage in
comparision to the negative control (solvent). On the other hand, after 40
seconds of photopolymerisation of both adhesives in all tested
concentrations, the degree of DNA damage in Comet assay had no significant
difference (P>0.05, ?2 test) compared to the negative control. Therefore,
only the 40 seconds of photopolymerisation prevented genotoxic effects of
both adhesives in the Comet assay.