Evidence of gastric ulcer healing activity of Maytenus robusta Reissek: In vitro and in vivo studies

VEGF is a highly specific stimulator of endothelial cells and may play an important role in angiogenesis in the process of tissue regeneration. We previously showed that cyclooxygenase-2 (COX-2) expressed in mesenchymal cells of the ulcer bed is involved in the ulcer repair process. To clarify the role of COX-2 in angiogenesis during gastric ulcer healing, we investigated the relation between COX-2 expression and VEGF production in human gastric fibroblasts in vivo and in vitro. Gastric fibroblasts were cultured in RPMI 1640 with and without IL-1α or IL-1β in the presence or absence of NS-398, a selective COX-2 inhibitor. Supernatant VEGF and PGE2 concentrations were measured by enzyme-linked immunosorbent assay. COX-2 expression in fibroblasts was determined by Western blot analysis. VEGF and COX-2 expression in surgical resections of human gastric ulcer tissue was examined immunohistochemically. IL-1 dose dependently enhanced VEGF release in cultured gastric fibroblasts after a 24-h stimulation. IL-1 also stimulated PGE2 production in gastric fibroblasts via COX-2 induction. NS-398 significantly suppressed VEGF and PGE2 release from IL-1-stimulated gastric fibroblasts; concurrent addition of PGE2 restored NS-398-inhibited VEGF release. COX-2 and VEGF immunoreactivity were colocalized in fibroblast-like cells in the ulcer bed of gastric tissues. These results suggest that COX-2 plays a key role in VEGF production in gastric fibroblasts stimulated by IL-1 in vitro and that angiogenesis induced by the COX-2-VEGF pathway might be involved in gastric ulcer healing.

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The Possible Role of Vascular Endothelial Growth Factor (VEGF) in Gastric Ulcer Healing: Effect of Sofalcone on VEGF Release In Vitro

Journal of Clinical Gastroenterology ◽

10.1097/00004836-199800001-00029 ◽

1998 ◽

Vol 27 ◽

pp. S178-S182 ◽

Cited By ~ 9

Author(s):

Morio Takahashi ◽

Shin Maeda ◽

Keiji Ogura ◽

Akira Terano ◽

Masao Omata

Keyword(s):

Vascular Endothelial Growth Factor ◽

Gastric Ulcer ◽

Growth Factor ◽

Ulcer Healing ◽

Vascular Endothelial ◽

Gastric Ulcer Healing ◽

Endothelial Growth Factor ◽

Vegf Release

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Chrysophyllum Albidum Accelerates Delayed Gastric Ulcer Healing in Rats Through Oxidative Stress Reversal and Proton Pump Inhibition

Nigerian Journal of Pharmaceutical Research ◽

10.4314/njpr.v16i2.7 ◽

2021 ◽

Vol 16 (2) ◽

pp. 163-175

Author(s):

A.T. Salami ◽

A. D. Famurewa ◽

T. P Omayone ◽

T. F. Iyiola ◽

S. B. Olaleye

Keyword(s):

Gastric Ulcer ◽

Proton Pump ◽

Ulcer Healing ◽

Positive Control ◽

Negative Control ◽

Gastric Ulcer Healing ◽

Proton Pump Inhibition ◽

Male Wistar Rats ◽

Underlying Mechanisms

Background: Chrysophyllum albidum has been documented to exert its gastric ulcer (GU) healing activities by modulating blood inflammatory mediators, however, other probable in-vivo underlying mechanisms are still vague which this study sought to investigate.Materials and Methods: Male Wistar rats (120-130g) divided into 9 groups (n=15 for groups I-VII; n=5 for groups VIII & IX) viz: Groups I- positive control (DUnA); II and III–250 and 500mg/kg methanolic extract of C. albidum (MeCaB) bark respectively; IV, V and VI-100mg/kg fractions A, B and C respectively; VII–30mg/kg omeprazole; VIII-ulcerated untreated (baseline), IX-negative control. Chronic GU was induced experimentally and delayed using indomethacin with 14 days simultaneous drug treatment. Gastric ulcer score, mucin content, antioxidant and proton pump activities were evaluated by days 3, 7 and 14 of treatment. Data were expressed as Mean+SEM and P<0.05 was significant.Results: C. albidum and fractions treated groups significantly decreased gastric ulcer scores and lipid peroxidation compared with DUnA. Negative control, C. albidum and fraction treated groups significantly increased superoxide dismutase, catalase, glutathione levels and mucin content compared with DUnA group by days 3 and 7. C. albidum, Negative and baseline control groups significantly decreased H+K+ATPase activities compared with DUnA by day14.Conclusion: C. albidum and its fractions facilitated the healing of gastric ulcer, probably by enhanced antioxidant levels, mucin content and decreased gastric H+K+ATPase activity. Keywords: C. albidum and chromatographic fractions, gastric ulcer healing, mucin , antioxidant, H+/K+ATPase pump.

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Calendula officinalis L. inflorescences extract: in vivo evaluation of its gastric ulcer healing potential

Journal Biomedical and Biopharmaceutical Research ◽

10.19277/bbr.17.1.230 ◽

2020 ◽

Vol 17 (1) ◽

pp. 1-15

Author(s):

Luisa Mota da Silva ◽

Viviane Silva Bunn ◽

Luisa Nathalia Bolda Mariano ◽

Lincon Bordignon Somensi ◽

Luiz Carlos Klein Jr. ◽

...

Keyword(s):

Gastric Ulcer ◽

Ulcer Healing ◽

Calendula Officinalis ◽

In Vivo Evaluation ◽

Gastric Ulcer Healing ◽

Calendula Officinalis L

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Bacterial colonization and healing of gastric ulcers: the effects of epidermal growth factor

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.2000.278.1.g105 ◽

2000 ◽

Vol 278 (1) ◽

pp. G105-G112 ◽

Cited By ~ 15

Author(s):

Susan N. Elliott ◽

J. L. Wallace ◽

W. McKnight ◽

D. G. Gall ◽

J. A. Hardin ◽

...

Keyword(s):

Gastric Ulcer ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Ulcer Healing ◽

Bacterial Colonization ◽

Neutrophil Infiltration ◽

Gastric Ulcers ◽

Gastric Ulcer Healing ◽

Epidermal Growth

.—Experimental gastric ulcers are rapidly colonized by various bacteria, resulting in significantly impaired healing. Epidermal growth factor (EGF) is capable of preventing bacterial colonization of the healthy intestinal mucosa. In this study, we examined the possibility that EGF accelerates gastric ulcer healing by reducing bacterial colonization of the ulcer. Gastric ulcers were induced by serosal application of acetic acid. The effect of daily administration of EGF on ulcer healing and bacterial colonization was assessed and compared with the effect of daily treatment with broad-spectrum antibiotics. EGF administration reduced colonization levels and accelerated ulcer healing as effectively as the antibiotic treatment. EGF was without effect on acid secretion or neutrophil infiltration into the ulcer. Bacterial growth was not inhibited in the presence of EGF in vitro. These results demonstrate that EGF reduces bacterial colonization during an established infection of a compromised mucosal surface. This effect may contribute to the ability of EGF to accelerate gastric ulcer healing. This effect is acid independent and not due to an anti-inflammatory effect or to direct bactericidal actions.

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Fabrication of ulcer-adhesive oral keratin hydrogel for gastric ulcer healing in a rat

Regenerative Biomaterials ◽

10.1093/rb/rbab008 ◽

2021 ◽

Vol 8 (2) ◽

Author(s):

Zhongjun Cheng ◽

Rui Qing ◽

Shilei Hao ◽

Yi Ding ◽

Haimeng Yin ◽

...

Keyword(s):

Gastric Ulcer ◽

Ulcer Healing ◽

High Viscosity ◽

Rat Stomach ◽

Gastric Ulcer Healing ◽

Research Findings ◽

Endoscopic Guidance ◽

In Suit ◽

Extracellular Matrix Scaffold

Abstract Hydrogel has been used for in suit gastric ulcer therapy by stopping bleeding, separating from ulcer from gastric fluids and providing extracellular matrix scaffold for tissue regeneration, however, this treatment guided with endoscopic catheter in most cases. Here, we developed an oral keratin hydrogel to accelerate the ulcer healing without endoscopic guidance, which can specially adhere to the ulcer because of the high-viscosity gel formation on the wound surface in vivo. Approximately 50% of the ulcer-adhesive keratin hydrogel can resident in ethanol-treated rat stomach within 12 h, while approximately 18% of them maintained in health rat stomach in the same amount of time. Furthermore, Keratin hydrogels accelerated the ethanol-induced gastric ulcer healing by stopping the bleeding, preventing the epithelium cells from gastric acid damage, suppressing inflammation and promoting re-epithelization. The oral administration of keratin hydrogel in gastric ulcer treatment can enhance the patient compliance and reduce the gastroscopy complications. Our research findings reveal a promising biomaterial-based approach for treating gastrointestinal ulcers.

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