scholarly journals Fabrication of ulcer-adhesive oral keratin hydrogel for gastric ulcer healing in a rat

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Zhongjun Cheng ◽  
Rui Qing ◽  
Shilei Hao ◽  
Yi Ding ◽  
Haimeng Yin ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
High Viscosity ◽  
Rat Stomach ◽  
Gastric Ulcer Healing ◽  
Research Findings ◽  
Endoscopic Guidance ◽  
In Suit ◽  
Extracellular Matrix Scaffold

Abstract Hydrogel has been used for in suit gastric ulcer therapy by stopping bleeding, separating from ulcer from gastric fluids and providing extracellular matrix scaffold for tissue regeneration, however, this treatment guided with endoscopic catheter in most cases. Here, we developed an oral keratin hydrogel to accelerate the ulcer healing without endoscopic guidance, which can specially adhere to the ulcer because of the high-viscosity gel formation on the wound surface in vivo. Approximately 50% of the ulcer-adhesive keratin hydrogel can resident in ethanol-treated rat stomach within 12 h, while approximately 18% of them maintained in health rat stomach in the same amount of time. Furthermore, Keratin hydrogels accelerated the ethanol-induced gastric ulcer healing by stopping the bleeding, preventing the epithelium cells from gastric acid damage, suppressing inflammation and promoting re-epithelization. The oral administration of keratin hydrogel in gastric ulcer treatment can enhance the patient compliance and reduce the gastroscopy complications. Our research findings reveal a promising biomaterial-based approach for treating gastrointestinal ulcers.

Cyclooxygenase-2-regulated vascular endothelial growth factor release in gastric fibroblasts

2004 ◽  
Vol 287 (2) ◽  
pp. G444-G451 ◽  
Author(s):  
Shuhei Miura ◽  
Atsushi Tatsuguchi ◽  
Ken Wada ◽  
Hiroki Takeyama ◽  
Yoko Shinji ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Repair Process ◽  
Cyclooxygenase 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gastric Ulcer Healing ◽  
Cox 2 ◽  
Vegf Release

VEGF is a highly specific stimulator of endothelial cells and may play an important role in angiogenesis in the process of tissue regeneration. We previously showed that cyclooxygenase-2 (COX-2) expressed in mesenchymal cells of the ulcer bed is involved in the ulcer repair process. To clarify the role of COX-2 in angiogenesis during gastric ulcer healing, we investigated the relation between COX-2 expression and VEGF production in human gastric fibroblasts in vivo and in vitro. Gastric fibroblasts were cultured in RPMI 1640 with and without IL-1α or IL-1β in the presence or absence of NS-398, a selective COX-2 inhibitor. Supernatant VEGF and PGE2 concentrations were measured by enzyme-linked immunosorbent assay. COX-2 expression in fibroblasts was determined by Western blot analysis. VEGF and COX-2 expression in surgical resections of human gastric ulcer tissue was examined immunohistochemically. IL-1 dose dependently enhanced VEGF release in cultured gastric fibroblasts after a 24-h stimulation. IL-1 also stimulated PGE2 production in gastric fibroblasts via COX-2 induction. NS-398 significantly suppressed VEGF and PGE2 release from IL-1-stimulated gastric fibroblasts; concurrent addition of PGE2 restored NS-398-inhibited VEGF release. COX-2 and VEGF immunoreactivity were colocalized in fibroblast-like cells in the ulcer bed of gastric tissues. These results suggest that COX-2 plays a key role in VEGF production in gastric fibroblasts stimulated by IL-1 in vitro and that angiogenesis induced by the COX-2-VEGF pathway might be involved in gastric ulcer healing.

scholarly journals Chrysophyllum Albidum Accelerates Delayed Gastric Ulcer Healing in Rats Through Oxidative Stress Reversal and Proton Pump Inhibition

2021 ◽  
Vol 16 (2) ◽  
pp. 163-175
Author(s):  
A.T. Salami ◽  
A. D. Famurewa ◽  
T. P Omayone ◽  
T. F. Iyiola ◽  
S. B. Olaleye
Keyword(s):  
Gastric Ulcer ◽  
Proton Pump ◽  
Ulcer Healing ◽  
Positive Control ◽  
Negative Control ◽  
Gastric Ulcer Healing ◽  
Proton Pump Inhibition ◽  
Male Wistar Rats ◽  
Underlying Mechanisms

Background: Chrysophyllum albidum has been documented to exert its gastric ulcer (GU) healing activities by modulating blood inflammatory mediators, however, other probable in-vivo underlying mechanisms are still vague which this study sought to investigate.Materials and Methods: Male Wistar rats (120-130g) divided into 9 groups (n=15 for groups I-VII; n=5 for groups VIII & IX) viz: Groups I- positive control (DUnA); II and III–250 and 500mg/kg methanolic extract of C. albidum (MeCaB) bark respectively; IV, V and VI-100mg/kg fractions A, B and C respectively; VII–30mg/kg omeprazole; VIII-ulcerated untreated (baseline), IX-negative control. Chronic GU was induced experimentally and delayed using indomethacin with 14 days simultaneous drug treatment. Gastric ulcer score, mucin content, antioxidant and proton pump activities were evaluated by days 3, 7 and 14 of treatment. Data were expressed as Mean+SEM and P<0.05 was significant.Results: C. albidum and fractions treated groups significantly decreased gastric ulcer scores and lipid peroxidation compared with DUnA. Negative control, C. albidum and fraction treated groups significantly increased superoxide dismutase, catalase, glutathione levels and mucin content compared with DUnA group by days 3 and 7. C. albidum, Negative and baseline control groups significantly decreased H+K+ATPase activities compared with DUnA by day14.Conclusion: C. albidum and its fractions facilitated the healing of gastric ulcer, probably by enhanced antioxidant levels, mucin content and decreased gastric H+K+ATPase activity. Keywords: C. albidum and chromatographic fractions, gastric ulcer healing, mucin , antioxidant, H+/K+ATPase pump.

scholarly journals Calendula officinalis L. inflorescences extract: in vivo evaluation of its gastric ulcer healing potential

2020 ◽  
Vol 17 (1) ◽  
pp. 1-15
Author(s):  
Luisa Mota da Silva ◽  
Viviane Silva Bunn ◽  
Luisa Nathalia Bolda Mariano ◽  
Lincon Bordignon Somensi ◽  
Luiz Carlos Klein Jr. ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Calendula Officinalis ◽  
In Vivo Evaluation ◽  
Gastric Ulcer Healing ◽  
Calendula Officinalis L

scholarly journals Evidence of gastric ulcer healing activity of Maytenus robusta Reissek: In vitro and in vivo studies

2015 ◽  
Vol 175 ◽  
pp. 75-85 ◽  
Author(s):  
Luisa Mota da Silva ◽  
Thaise Boeing ◽  
Lincon Bordignon Somensi ◽  
Benhur Judah Cury ◽  
Viviane Miranda Bispo Steimbach ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
In Vivo Studies ◽  
Gastric Ulcer Healing

Gastric ulcer healing is regulated by platelets through endostatin release: Modulation by ticlopidine, aspirin and celecoxib

2001 ◽  
Vol 120 (5) ◽  
pp. A143-A143
Author(s):  
L MA ◽  
S ELLIOTT ◽  
G CIRINO ◽  
A BURET ◽  
J WALLACE
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Gastric Ulcer Healing

Gastric ulcer healing is regulated by platelets through endostatin release: Modulation by ticlopidine, aspirin and celecoxib

2001 ◽  
Vol 120 (5) ◽  
pp. A143
Author(s):  
Li Ma ◽  
Susan N. Elliott ◽  
Giuseppe Cirino ◽  
Andre Buret ◽  
John L. Wallace
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Gastric Ulcer Healing

Chronic gastric ulcer healing in rats subjected to selective and non-selective cyclooxygenase-2 inhibitors

2002 ◽  
Vol 442 (1-2) ◽  
pp. 125-135 ◽  
Author(s):  
Bettina Berenguer ◽  
Catalina Alarcón de la Lastra ◽  
Francisco Javier Moreno ◽  
Maria José Martı́n
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Cyclooxygenase 2 ◽  
Chronic Gastric Ulcer ◽  
Gastric Ulcer Healing ◽  
Cyclooxygenase 2 Inhibitors

Role of nuclear factor-κB in gastric ulcer healing in rats

2001 ◽  
Vol 280 (6) ◽  
pp. G1296-G1304 ◽  
Author(s):  
Satoru Takahashi ◽  
Takuya Fujita ◽  
Akira Yamamoto
Keyword(s):  
Gastric Ulcer ◽  
Mrna Expression ◽  
Ulcer Healing ◽  
Nuclear Factor Κb ◽  
Normal Mucosa ◽  
Cyclooxygenase 2 ◽  
Interleukin 1Β ◽  
Nuclear Factor ◽  
Gastric Ulcer Healing

We investigated the role of nuclear factor-κB (NF-κB) in gastric ulcer healing in rats. NF-κB was activated in ulcerated tissue but not in normal mucosa, and the level of the activation was decreased with ulcer healing. NF-κB activation was observed in fibroblasts, monocytes/macrophages, and neutrophils. Treatment of gastric fibroblasts, isolated from the ulcer base, with interleukin-1β activated NF-κB and the subsequently induced cyclooxygenase-2 and cytokine-induced neutrophil chemoattractant-1 (CINC-1) mRNA expression. Inhibition of activated NF-κB action resulted in suppression of both their mRNA expression and increases in PGE2 and CINC-1 levels induced by interleukin-1β. Persistent prevention of NF-κB activation caused an impairment of ulcer healing in rats. Gene expression of interleukin-1β, CINC-1, cyclooxygenase-2, and inducible nitric oxide synthase in ulcerated tissue had been inhibited before the delay in ulcer healing became manifest. The increased levels of cyclooxygenase-2 protein and PGE2 production were also reduced. These results demonstrate that NF-κB, activated in ulcerated tissue, might upregulate the expression of healing-promoting factors responsible for gastric ulcer healing in rats.

scholarly journals ANTIOXIDANT AND GASTRIC ULCER HEALING EFFECT OF ORTHOSIPHONSTAMINEUS (BENTH.) LEAVES EXTRACT IN ASPIRIN-INDUCED RATS

2017 ◽  
Vol 10 (2) ◽  
pp. 397 ◽  
Author(s):  
Ari - Yuniarto
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Control Group ◽  
Histopathological Analysis ◽  
Antiulcer Activity ◽  
Gastric Ulcer Healing ◽  
Orthosiphon Stamineus ◽  
Mucosal Integrity ◽  
Healing Effect ◽  
Dpph Method

Objective: In the gastrointestinal system, gastric ulcer is one of the common serious problems in human life and gives contribution against morbidity and mortality incidence. The pathophysiology of gastric ulcer is an imbalance between aggressive factor and mucosal integrity factor. Increase of aggressive factors and decrease of mucosal integrity factors have potential against developed of gastric ulcer disease. The objective of the research was to evaluate antioxidant and gastric ulcer healing effect of Orthosiphon stamineus (Benth.) leaves extract in aspirin-induced rats.Methods: In vivo antiulcer activity of Orthosiphon leaves extract was evaluated through several parameters involves gastric acidity, number of ulcers, diameters of ulcers, ulcer index (UI), and healing ratio. Dose level of Orthosiphon leaves extract which used in this study such as 250 and500 mg/kg, respectively. Antioxidant activity of Orthosiphon leaves extract was evaluated by 1,1-diphenyl-2-picrylhydrazyl hydrate (DPPH) method. Histopathological of the stomach was performed using hematoxylin-eosin stained.Results: The results of the study showed that groups which given Orthosiphon leaves extract have significantly different for gastric ulcer healing compared to the control group and were supported by histopathological analysis. The Orthosiphon leaves extract also showed maximum scavengingactivity at a concentration of 100 µg/ml (58.86% inhibition) and minimum at 50 μg/ml (29.60% inhibition) with inhibitory concentration 50% (IC  )50 84.54 µg/ml when compared to ascorbic acid as the standard with IC5.08 µg/ml by DPPH method.Conclusion: It can be concluded that from the experimental study of O. stamineus (Benth.) leaves extract has potential antiulcer activity in aspirin- induced rats model and antioxidant effect using DPPH method. Stomach tissues regeneration in gastric ulcer model might be affected by the improvement of antioxidant status.Keywords: Orthosiphon stamineus (Benth.), Extract, Ulcer, Aspirin, 1,1-diphenyl-2-picrylhydrazyl hydrate.

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