Antidiabetic effects of Olea europaea L. leaves in diabetic rats induced by high-fat diet and low-dose streptozotocin

Sequoyitol decreases blood glucose, improves glucose intolerance, and enhances insulin signaling in ob/ob mice. The aim of this study was to investigate the effects of sequoyitol on diabetic nephropathy in rats with type 2 diabetes mellitus and the mechanism of action. Diabetic rats, induced with a high-fat diet and a low dose of streptozotocin, and were administered sequoyitol (12.5, 25.0, and 50.0 mg·(kg body mass)−1·d−1) for 6 weeks. The levels of fasting blood glucose (FBG), serum insulin, blood urea nitrogen (BUN), and serum creatinine (SCr) were measured. The expression levels of p22phox, p47phox, NF-κB, and TGF-β1 were measured using immunohistochemisty, real-time PCR, and (or) Western blot. The total antioxidative capacity (T-AOC), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were also determined. The results showed that sequoyitol significantly decreased FBG, BUN, and SCr levels, and increased the insulin levels in diabetic rats. The level of T-AOC was significantly increased, while ROS and MDA levels and the expression of p22phox, p47phox, NF-κB, and TGF-β1 were decreased with sequoyitol treatment both in vivo and in vitro. These results suggested that sequoyitol ameliorates the progression of diabetic nephropathy in rats, as induced by a high-fat diet and a low dose of streptozotocin, through its glucose-lowering effects, antioxidant activity, and regulation of TGF-β1 expression.

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The Effect of Long-Term Intranasal Serotonin Treatment on Metabolic Parameters and Hormonal Signaling in Rats with High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes

International Journal of Endocrinology ◽

10.1155/2015/245459 ◽

2015 ◽

Vol 2015 ◽

pp. 1-17 ◽

Cited By ~ 33

Author(s):

Kira V. Derkach ◽

Vera M. Bondareva ◽

Oxana V. Chistyakova ◽

Lev M. Berstein ◽

Alexander O. Shpakov

Keyword(s):

Type 2 Diabetes ◽

High Fat Diet ◽

Low Dose ◽

Diabetic Rats ◽

Brain Serotonin ◽

Serotonin Level ◽

High Fat ◽

Brain Serotonin Level ◽

The Brain

In the last years the treatment of type 2 diabetes mellitus (DM2) was carried out using regulators of the brain signaling systems. In DM2 the level of the brain serotonin is reduced. So far, the effect of the increase of the brain serotonin level on DM2-induced metabolic and hormonal abnormalities has been studied scarcely. The present work was undertaken with the aim of filling this gap. DM2 was induced in male rats by 150-day high-fat diet and the treatment with low dose of streptozotocin (25 mg/kg) on the 70th day of experiment. From the 90th day, diabetic rats received for two months intranasal serotonin (IS) at a daily dose of 20 μg/rat. The IS treatment of diabetic rats decreased the body weight, and improved glucose tolerance, insulin-induced glucose utilization, and lipid metabolism. Besides, it restored hormonal regulation of adenylyl cyclase (AC) activity in the hypothalamus and normalized AC stimulation byβ-adrenergic agonists in the myocardium. In nondiabetic rats the same treatment induced metabolic and hormonal alterations, some of which were similar to those in DM2 but expressed to a lesser extent. In conclusion, the elevation of the brain serotonin level may be regarded as an effective approach to treat DM2 and its complications.

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Antidiabetic effect of Ficus racemosa Linn. stem bark in high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats: A mechanistic study

Food Chemistry ◽

10.1016/j.foodchem.2011.10.052 ◽

2012 ◽

Vol 132 (1) ◽

pp. 186-193 ◽

Cited By ~ 59

Author(s):

V.P. Veerapur ◽

K.R. Prabhakar ◽

B.S. Thippeswamy ◽

Punit Bansal ◽

K.K. Srinivasan ◽

...

Keyword(s):

High Fat Diet ◽

Low Dose ◽

Stem Bark ◽

Diabetic Rats ◽

Mechanistic Study ◽

High Fat ◽

Antidiabetic Effect ◽

Ficus Racemosa ◽

Type 2 Diabetic

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Antidiabetic effects of pterostilbene through PI3K/Akt signal pathway in high fat diet and STZ-induced diabetic rats

European Journal of Pharmacology ◽

10.1016/j.ejphar.2019.172526 ◽

2019 ◽

Vol 859 ◽

pp. 172526 ◽

Cited By ~ 13

Author(s):

Hualei Sun ◽

Xinxin Liu ◽

Shao Rong Long ◽

Teng wang ◽

Huina Ge ◽

...

Keyword(s):

High Fat Diet ◽

Diabetic Rats ◽

Signal Pathway ◽

High Fat ◽

Antidiabetic Effects

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Antidiabetic effects of malonyl ginsenosides from Panax ginseng on type 2 diabetic rats induced by high-fat diet and streptozotocin

Journal of Ethnopharmacology ◽

10.1016/j.jep.2012.10.058 ◽

2013 ◽

Vol 145 (1) ◽

pp. 233-240 ◽

Cited By ~ 72

Author(s):

Zhi Liu ◽

Wei Li ◽

Xiang Li ◽

Ming Zhang ◽

Li Chen ◽

...

Keyword(s):

Panax Ginseng ◽

High Fat Diet ◽

Diabetic Rats ◽

High Fat ◽

Type 2 Diabetic ◽

Antidiabetic Effects

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Exercise training prevents the development of cardiac dysfunction in the low-dose streptozotocin diabetic rats fed a high-fat diet

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2012-0294 ◽

2013 ◽

Vol 91 (1) ◽

pp. 80-89 ◽

Cited By ~ 21

Author(s):

Riley A. Epp ◽

Shanel E. Susser ◽

Marc P. Morissette ◽

D. Scott Kehler ◽

Davinder S. Jassal ◽

...

Keyword(s):

Sarcoplasmic Reticulum ◽

Exercise Training ◽

Protein Content ◽

Cardiac Dysfunction ◽

High Fat Diet ◽

Low Dose ◽

Diabetic Rats ◽

Left Ventricular ◽

High Fat ◽

Altered Expression

This study tested the hypothesis that exercise training would prevent the development of diabetes-induced cardiac dysfunction and altered expression of sarcoplasmic reticulum Ca2 +-transport proteins in the low-dose streptozotocin-induced diabetic rats fed a high-fat diet (HFD+STZ). Male Sprague–Dawley rats (4 weeks old; 125–150 g) were made diabetic using a high-fat diet (40% fat, w/w) and a low-dose of streptozotocin (35 mg·(kg body mass)–1) by intravenous injection. Diabetic animals were divided among a sedentary group (Sed+HFD+STZ) or an exercise-trained group (Ex+HFD+STZ) that accumulated 3554 ± 338 m·day–1 of voluntary wheel running (mean ± SE). Sedentary animals fed a low-fat diet served as the control (Sed+LFD). Oral glucose tolerance was impaired in the sedentary diabetic group (1179 ± 29; area under the curve (a.u.c.)) compared with that in the sedentary control animals (1447 ± 42 a.u.c.). Although left ventricular systolic function was unchanged by diabetes, impaired E/A ratios (i.e., diastolic function) and rates of pressure decay (–dP/dt) indicated the presence of diastolic dysfunction. Diabetes also reduced SERCA2a protein content and maximal SERCA2a activity (Vmax) by 21% and 32%, respectively. In contrast, the change in each parameter was attenuated by exercise training. Based on these data, it appears that exercise training prevented the development of diabetic cardiomyopathy and the dysregulation of sarcoplasmic reticulum protein content in an inducible animal model of type 2 diabetes.

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