Serum Progesterone and Conception Rates in Acyclic Embryo Recipient Mares Using a Bovine Progesterone-Releasing Intravaginal Device

2021 ◽  
Vol 97 ◽  
pp. 103325
Author(s):  
Wilson Luis Rigoleto Júnior ◽  
Waldomiro Dias de Camargo Neto ◽  
Guilherme de Paula Nogueira ◽  
Tiago Augusto Ferrari ◽  
Claudia Maria Bertan Membrive ◽  
...  
Keyword(s):  
Serum Progesterone ◽  
Conception Rates ◽  
Intravaginal Device

8 EFFECT OF SEMEN TYPES (SEXED v. NONSEXED) ON CONCEPTION RATES OF GIROLANDO HEIFERS INSEMINATED IN FIXED-TIME AI

2010 ◽  
Vol 22 (1) ◽  
pp. 162
Author(s):  
J. Almeida ◽  
O. A. Resende ◽  
D. L. Silveira
Keyword(s):  
Body Condition Score ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
São Paulo ◽  
Device Removal ◽  
Conception Rates ◽  
Sexed Semen ◽  
Condition Score ◽  
Intravaginal Device

With the goal of optimizing protocols, fixed-time artificial insemination (FTAI) was performed under field conditions in a study to evaluate conception rates in Girolando (5/8) heifers (18 to 24 months of age) treated with estradiol benzoate (BE) to induce ovulation. Forty heifers were allocated to 2 groups with 20 animals each: GI-sexed semen (2 million spermatozoa/dose) and GII-nonsexed semen (20 million spermatozoa/dose), with body condition score (BCS) of 3.0 ± 0.14 (scale of 1 to 5), middleweight of 335 ± 38 kg, so that it was made for reutilization of progesterone device. On Day 0 (morning), the animals received 2.0 mg of BE (RIC-BE®,Tecnopec, São Paulo, Brazil), 0.075 mg of D-cloprostenol (Prolise®, ARSA S.R.L., Buenos Aires, Argentina), and an intravaginal device of 1 g of progesterone (Primer®, Tecnopec). On Day 8 (morning) the device was removed and 0.075 mg of D-cloprostenol was managed in the GI animals, then these animals received 1.0 mg of BE 24 h after device removal. The insemination was in the late afternoon of Day 10 with a 6-hour delay (4 to 6 pm). The GII animals received Primer previously used for 8 days (being used in the GI animals under the same protocol). After pregnancy diagnosis at 60 days, a third group was created (GIII) using the not-pregnant animals of GI and GII. In GIII, Primer from third use [disinfected in solution Kilol®-L 1:250 (Quinabra, São Paulo, Brazil) and rinsed with water, dried, and packed in plastic sacks and kept for 60 days] was used following the GI protocol with sexed semen. There were no significant differences (P > 0.05) in the conception rates of 40, 58, and 65% among groups GI (8/20), GII (11/20), and GIII (13/20). These results indicate very good conception rates for heifers, contrary to recommendations of commercial companies of sexed semen that alert for the conception rate for mated animals to be lower when using FTAI and mating protocols in mass, compared with that of AI after estrus observation.

Effect of combined exogenous progesterone with luteotrophic support via equine chorionic gonadotrophin (eCG) on corpus luteum development, circulating progesterone concentrations and embryo development in cattle

2016 ◽  
Vol 28 (3) ◽  
pp. 269 ◽  
Author(s):  
L. O'Hara ◽  
N. Forde ◽  
P. Duffy ◽  
F. Randi ◽  
A. K. Kelly ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Embryo Quality ◽  
Single Injection ◽  
Chorionic Gonadotrophin ◽  
Serum Progesterone ◽  
Conceptus Development ◽  
Luteal Tissue ◽  
Exogenous Progesterone ◽  
Intravaginal Device

The aim was to examine the effect of a single intramuscular (i.m.) injection of equine chorionic gonadotrophin (eCG) on Day 3 after oestrus on corpus luteum (CL) development, circulating progesterone and conceptus development in cross-bred beef heifers. In Experiment 1, heifers received: (1) saline, or a single i.m. injection of eCG on Day 3 at (2) 250 IU (3) 500 IU (4) 750 IU or (5) 1000 IU. Administration of eCG resulted in increased luteal tissue area and progesterone and oestradiol concentrations compared with controls. In Experiment 2, heifers received (1) a progesterone-releasing intravaginal device (PRID Delta) from Day 3 to 5 or (2) a PRID Delta from Day 3 to 5 plus a single injection of 750 IU eCG on Day 3. In vitro-produced blastocysts (n = 10 per recipient) were transferred on Day 7 and heifers were slaughtered on Day 14 to assess conceptus development. Administration of eCG reduced the number of short cycles (6.3% vs 31.3%) and increased mean luteal tissue weight (P = 0.02). Insertion of a PRID Delta on Day 3 resulted in an elevation (P < 0.05) in serum progesterone until removal on Day 5. Administration of eCG at the time of PRID Delta insertion resulted in higher progesterone levels (P < 0.05) from Day 10 onwards. Conceptus dimensions were not affected. In conclusion, a single injection of eCG on Day 3 increased CL size and progesterone concentrations and, when given in conjunction with a progesterone-releasing device, appeared to reduce the number of short cycles, presumably due to its luteotrophic nature. The implications of the elevated oestradiol concentrations for embryo quality require further study.

17 PREGNANCY RATE OBTAINED WITH THE REUTILIZATION OF INTRAVAGINAL DEVICE DICO AFTER 6 DAYS OF TREATMENT FOR ESTRUS SYNCHRONIZATION IN SHEEP

2011 ◽  
Vol 23 (1) ◽  
pp. 115
Author(s):  
P. C. dos Santos-Neto ◽  
C. García-Pintos ◽  
A. Menchaca
Keyword(s):  
Pregnancy Rate ◽  
Body Condition Score ◽  
Intrauterine Insemination ◽  
Prostaglandin F2α ◽  
Follicular Development ◽  
Device Removal ◽  
Fertility Rates ◽  
Short Term ◽  
Serum Progesterone ◽  
Intravaginal Device

The new intravaginal device DICO (Dispositivo Intravaginal Caprino Ovino, 0.3 mg progesterone, Syntex, Buenos Aires, Argentina) is effective in controlling serum progesterone concentrations, follicular development and time of ovulation in sheep, even in its second use after 6 days of treatment (Vilariño et al. 2010 Small Rumin. Res. 91, 219–224). The pregnancy rate obtained with DICO in its first use is similar to that obtained with CIDR-G (Pfizer, New York, NY, USA) (abstract of García-Pintos et al. in this issue). However, pregnancy rate with the reutilisation of DICO has not been evaluated yet. The aim of this study was to determine fertility rates in ewes synchronized with a reused DICO in comparison with a reused CIDR-G. The experiment was carried out on 314 Corriedale multiparous ewes during breeding season (March, 33°S, Uruguay), with a body condition score of 3.0 ± 0.1 (scale 0–5). Ewes received a short-term-protocol using DICO (n = 139) or CIDR-G (n = 175) during 6 days, previously used for 6 days. One dose of prostaglandin F2α analogue (125 μg cloprostenol, Ciclase DL, Syntex) and 300 IU eCG (Novormon, Syntex) were given at the time of device removal. Cervical insemination (200 million spermatozoa) or laparoscopic intrauterine insemination (100 million spermatozoa) was performed with fresh semen at fixed-time 48 or 54 h from device removal, respectively. Pregnancy diagnosis was done by transrectal ultrasonography (5 MHz, Well D, Beijing, China) 40 days after insemination. Pregnancy rate was analysed by logistic regression, and number of fetus/pregnant ewes and fetus/inseminated ewes was analysed by Poisson regression. No differences were found between reused DICO and CIDR-G devices (Table 1). Overall, pregnancy rate was affected by the method of insemination (P ≤ 0.05), being lower for cervical (37.0%; 57/154) than intrauterine insemination (55.0%; 88/160). In conclusion, the reutilization of DICO devices in the short-term protocol (6 days of treatment) allows similar fertility rates compared to re-used CIDR-G devices, by both cervical and intrauterine insemination. Table 1.Fertility rate obtained with the reutilisation of DICO and CIDR in short-term protocol (6 days of treatment) in sheep

Influence of hCG Administration after AI on Conception Rates and Serum Progesterone Concentration in Cattle

2007 ◽  
Vol 10 (16) ◽  
pp. 2709-2713 ◽  
Author(s):  
N. Shams-Esfandabadi . ◽  
A. Shirazi . ◽  
P. Mirshokrai . ◽  
M. Bonyadian .
Keyword(s):  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Conception Rates

17 INFLUENCE OF DIFFERENT CONCENTRATIONS OF PROGESTERONE DURING FIXED-TIME ARTIFICIAL INSEMINATION PROTOCOLS ON OVULATION AND PREGNANCY RATES IN NELORE HEIFERS

2008 ◽  
Vol 20 (1) ◽  
pp. 89
Author(s):  
M. F. Pegorer ◽  
R. L. Ereno ◽  
C. M. Barros
Keyword(s):  
Artificial Insemination ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
High Concentration ◽  
Serum Progesterone ◽  
Significant Difference ◽  
Intravaginal Device

Previous reports indicate that a high concentration of serum progesterone (P4) decreases LH pulse frequency (Burke et al. 1996 Anim. Reprod. Sci. 45, 13–28) and, consequently, follicular growth. This may reduce the efficiency of fixed-time artificial insemination (FTAI) protocols that utilize P4 in Bos indicus heifers (Marques et al. 2005 Acta Sci. Vet.). The objective of the present work was to evaluate the influence of different P4 concentrations on the ovulation and pregnancy rates of zebu heifers subjected to hormonal protocols for FTAI. Four hormonal protocols with different P4 concentrations were used to induce synchronization of ovulation in Nelore (Bos indicus) heifers (n = 292). Before the beginning of the treatments, the animals were subjected to ovary evaluations by ultrasonography (Aloka SSD 500, 5-MHz probe; Aloka, Tokyo, Japan) twice in an interval of 10 days. Only heifers with a corpus luteum (CL) in at least one of the ultrasonographic evaluations were utilized. At a random stage of the estrous cycle, the selected heifers received an intravaginal progesterone-releasing device containing 0.558 g of P4 [Triu�, Biogenesis, Buenos Aires, Argentina; Treatment 1 (T1); n = 76], or 1 g of P4 [Cronipress�, Biogenesis; Treatment 2 (T2); n = 70], or 0.558 g of P4 and prostaglandin F2α (PGF2α) [150 �g d-cloprostenol, IM, Croniben�, Biogenesis; Treatment 3 (T3), n = 75], or 1 g of P4 and PGF2α [Treatment 4 (T4), n = 71]. At the time of intravaginal device insertion (Day 0 = D0), all animals received 2 mg of estradiol benzoate (EB, Bioestrogen�, IM, Biogenesis), and PGF2α was administered only in animals from groups T3 and T4. Eight days later (D8), PGF2α (0.150 g d-cloprostenol) was administered to all heifers and the P4 source was removed. Twenty-four h after removal of the intravaginal device, the heifers were treated with EB (1 mg, IM), and 30 to 36 h later all animals were fixed-time inseminated (FTAI, D10), without estrus detection. In a subset of heifers from each group (T1 = 28; T2 = 34; T3 = 33; T4 = 31), ovarian ultrasonography was performed on D9 (at 8:00 A.M.) and D11 (at 14:00 P.M.) in order to determine ovulation rate after each treatment. The data were analyzed by logistic regression (GENPROC, SAS; SAS Institute, Inc., Cary, NC, USA). Ovulation rates after treatments T1, T2, T3, and T4, were, respectively, 71.4% (20/28), 76.5% (26/34), 84.9% (28/33), and 70.9% (22/31), and there was no significant difference among groups (P > 0.05). Pregnancy rates, ascertained by ultrasonography 40 days after AI, were 30.2% (23/76), 25.7% (18/70), 33.3% (25/75), and 28.2% (20/71), respectively, for T1, T2, T3, and T4, and likewise there was no significant difference among groups (P > 0.05). It is concluded that reduction of progesterone concentration in the intravaginal device (1 g v. 0.558 g) associated or not with administration of PGF2α at the beginning of treatments did not significantly influence ovulation and pregnancy rates in Nelore heifers submitted to a FTAI protocol. Additional experiments are underway to confirm or not the present results. This work was supported by FAPESP (Brazil) and BIOGENESIS (Argentina).

scholarly journals Use of Intravaginal Progesterone-Releasing Device Results in Similar Pregnancy Rates and Losses to Long-Acting Progesterone to Synchronize Acyclic Embryo Recipient Mares

2021 ◽  
Vol 8 (9) ◽  
pp. 190
Author(s):  
Lorenzo G. T. M. Segabinazzi ◽  
Luiz R. P. Andrade ◽  
Marco A. Alvarenga ◽  
Jose A. Dell’Aqua ◽  
Igor F. Canisso
Keyword(s):  
Single Dose ◽  
Pregnancy Rates ◽  
Sharp Decline ◽  
Serum Progesterone ◽  
Pregnancy Diagnosis ◽  
Release Device ◽  
Long Acting ◽  
Intravaginal Device ◽  
In Pregnancy

The objectives of this study were: (1) to assess uterine features and serum progesterone concentrations of acyclic mares synchronized and resynchronized with intravaginal progesterone release device (IPRD), and (2) to compare pregnancy rates and losses of cyclic and acyclic embryo recipient mares treated with different synchronization protocols. In Experiment 1, mares (n = 12) received estradiol for 3 days (E2-3d), and then 24 h after the last injection, an IPRD was inserted and kept in place for 9 days. Three days after IPRD removal, mares were treated with E2-3d, and then a new IPRD was inserted and maintained for three days. Serum progesterone concentrations were assessed 2, 6, and 12 h after insertion and removal of IPRD, and then daily from the insertion of the first IPRD to one day after removal of the second IPRD. Experiment 2 was conducted with embryo recipient mares randomly assigned to four groups: (1) Cyclic: mares (n = 75) had ovulation confirmed after receiving a single dose of histrelin when a periovulatory follicle was first detected, (2) LAP4: acyclic mares (n = 92) were treated with E2-3d and then administered a single dose of LAP4 24 h after the last estradiol injection, (3) IPRD: acyclic mares (n = 130) were treated with E2-3d and an IPRD for 4–8 days, and (4) RE-IPRD: acyclic mares (n = 32) were synchronized as in the IPRD group but not used for embryo transfer (ET), then 8 to 15 days later, the mares were resynchronized with E2-3d and an IPRD for 4–8 days. In vivo-produced Day-8 embryos were collected and transferred 4–8 days after ovulation or progesterone treatments. Mares in IPRD and RE-IPRD groups had the intravaginal device removed immediately before ET, and then a new IPRD was inserted right after ET. Pregnancy diagnosis was performed at 5, 30, and 60 days after ET. Once pregnancy was confirmed, mares in the three acyclic groups received weekly injections of LAP4 (1.5 g) until 120 days of pregnancy. Mares in IPRD and RE-IPRD groups had the device removed three days after the first pregnancy diagnosis. In Experiment 1, progesterone concentrations increased rapidly starting 2 h after insertion of IPRD (p < 0.05); then, concentrations plateaued well above pregnancy maintenance until removal on days 9 and 3, respectively. Progesterone concentrations were reduced to baseline 24 h after IPRD removal (p < 0.05). For experiment 2, there was no difference in pregnancy rates across groups (65–74%) or pregnancy losses by 60 days of gestation (7–12%) (p > 0.05). In conclusion, the IPRD used herein resulted in a rapid increase and a sharp decline in progesterone concentrations upon its insertion and removal, respectively. Finally, our results demonstrated that IPRD could be a compatible alternative to LAP4 to synchronize and resynchronize acyclic embryo recipient mares.

11 Early Induction of Luteolysis in Fixed-Time Artificial Insemination Protocols Increases Fertility in Beef Cows

2018 ◽  
Vol 30 (1) ◽  
pp. 145
Author(s):  
J. B. Borges ◽  
A. B. Machado ◽  
E. Pradebon ◽  
M. Dias ◽  
M. Bernardi ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Body Condition Score ◽  
Fixed Time ◽  
Beef Cows ◽  
Binary Outcomes ◽  
Serum Progesterone ◽  
Ovulatory Follicle ◽  
Condition Score ◽  
Prostaglandin F ◽  
Intravaginal Device

Complete luteolysis is essential for pregnancy success in beef cows submitted to fixed-time AI (FTAI) protocols. The objective of this study was to evaluate the effect of prostaglandin F (PGF) administration on Day 7 compared with Day 8 of an FTAI protocol on serum progesterone (P4) concentration, oestrus behaviour, ovulatory follicle (OF) diameter, and pregnancy per AI (P/AI). A total of 469 nonlactating Angus cows [with corpus luteum (CL), n = 359 or without CL, n = 110], body condition score 2.9 ± 0.2 (1 to 5 scale), maintained on pasture were used. Cows received a 1-g progesterone-releasing intravaginal device (Reproneo; GlobalGen, Jaboticabal, Brazil) and 2 mg of oestradiol benzoate i.m. (Syncrogen; GlobalGen) on Day 0. On Day 7, animals were randomly allocated into 2 groups. Cows were treated with 500 μg of sodium cloprostenol, IM (Inducio; GlobalGen), on Day 7 (PGF7, n = 238) or Day 8 (PGF8, n = 231). Then, P4 devices were removed and 0.5 mg of oestradiol cypionate, IM (Cipion; GlobalGen) was injected in all females on Day 8. The FTAI was performed 48 to 52 h after P4 device removal. Females had sacral region painted with appropriate paint (TELL TAIL, GEA, Hamilton New Zealand) on Day 8 to detect expression of oestrus. Transrectal ultrasonography (7.5-MHz linear transducer, MediSono P3V, Wilmington, DE, USA) was performed on Day 0 to detect presence of CL, on Day 10 to measure OF diameter, and on Day 40 to diagnose pregnancy. Blood samples were collected on Days 8 and 10 to measure P4 serum concentration by radioimmunoassay. Oestrus behaviour and pregnancy rate were analysed as binary outcomes using logistic regression (Proc GLIMMIX, SAS; SAS Institute Inc., Cary, NC, USA). OF diameter and P4 concentration were submitted to analysis of variance (Proc GLIMMIX, SAS). The oestrus manifestation was greater (odds ratio = 2.9; P = 0.0002) in females of PGF7 group (91.6%; 218/238) than PGF8 group (78.8%; 182/231). The P/AI of cows that exhibited oestrus was 60.2% (241/400) v. 39.1% (27/69), resulting in higher chance (odds ratio = 2.4) of pregnancy associated with oestrus behaviour (P = 0.0014). Diameter of the OF did not differ (P = 0.0881) between PGF7 (11.7 mm) and PGF8 (11.3 mm). The P/AI was higher (P = 0.0034) for PGF7 (63.9%, 152/238) v. PGF8 (50.2%, 116/231). In group PGF7, P4 on Day 8 did not differ between pregnant (1.7 ng mL−1) and nonpregnant (1.9 ng mL−1) females. However, group PGF8 pregnant females had lower P4 concentration (2.6 ng mL−1) on Day 8 (P = 0.0005) than nonpregnant (3.4 ng mL−1) females. On Day 10, P4 did not differ between treatments (PGF7 = 0.11 ng mL−1 v. PGF8 = 0.09 ng mL−1) and did not affect fertility (P = 0.2515). According to results, OF diameter and P4 concentration on Day 10 were not influenced by the day of PGF administration; however, earlier PGF injection resulted in higher oestrus behaviour and increased fertility.

Serum Progesterone, Oestradiol-17β, FSH and LH levels in Anestrus Surti buffaloes treated with Ovsynch alone and in combination with PRID

2018 ◽  
Vol 13 (03) ◽  
Author(s):  
H. R. Savani ◽  
C. T. Khasatiya ◽  
Swati Saxena ◽  
S. B. Patel ◽  
V. K. Singh
Keyword(s):  
Fixed Time ◽  
Control Group ◽  
Serum Progesterone ◽  
Treatment Groups ◽  
Serum Luteinizing Hormone ◽  
Significant Difference ◽  
The Mean ◽  
T1 And T2 ◽  
And Control ◽  
Intravaginal Device

This study was carried out on 18 postpartum anestrus Surti buffaloes, divided into three equal groups (6 in each) to evaluate the serum progesterone, oestradiol-17β, FSH and LH concentrations (by ELISA) before, during and after Ovsynch and Ovsynch + PRID protocols. Six anestrus buffaloes each were treated with standard Ovsynch protocol (T1) and Ovsynch along with PRID (Progesterone releasing intravaginal device) protocol (T2) with fixed-time AI (FTAI). Six anestrus buffaloes were kept as untreated control (T3). Blood samples were obtained from the animals on day 0 (before treatment), day 4 (during treatment), day 8 (after PGF2α injection), day of induced estrus/FTAI and day 18 post-AI (after treatment). The mean serum progesterone level on day 4 was significantly (p less than 0.01) higher in the T2 group as compared to T1 and T3 groups, while the level on day 8 was significantly (p less than 0.01) higher in both the treatment groups (T1 and T2) as compared to control group, however there was no significant difference (p>0.05) on the day of estrus between three groups. The mean serum oestradiol-17β and follicle stimulating hormone (FSH) levels on 8th day were significantly higher (p less than 0.01) in both the treatment groups (T1 and T2) as compared to control group (T3). However, there was no significant difference on day 0, 4, day of estrus and day 18 post-AI between groups. The mean serum luteinizing hormone (LH) levels of acyclic buffaloes were not found significantly different (p>0.05) among days in any of the treatment and control groups. But the mean LH concentration on the day of estrus in the T1, T2 and T3 was significantly higher (p less than 0.01) and thereafter decreased markedly on 18th day post-AI in all the groups. The conception rates at FTAI/first estrus in three groups were 66.66%, 50.00% and 75.00%, respectively.

SERUM PROGESTERONE AND INITIATION OF OVARIAN ACTIVITY IN PREPUBERAL HEIFERS TREATED WITH PROGESTERONE

1982 ◽  
Vol 62 (3) ◽  
pp. 759-766 ◽  
Author(s):  
R. RAJAMAHENDRAN ◽  
P. C. LAGUË ◽  
R. D. BAKER
Keyword(s):  
Time Of Day ◽  
Ovarian Activity ◽  
Rubber Tubing ◽  
Serum Progesterone ◽  
Hormonal Stimulation ◽  
Treatment Groups ◽  
Per Se ◽  
Single Implant ◽  
Intravaginal Device ◽  
Estradiol 17Β

The influence of progesterone on the initiation of ovarian activity in 32 prepuberal Holstein heifers (250 kg) was studied by inserting subcutaneous single or double Silastic implants (60-cm2 silicone rubber tubing containing 1 g progesterone or 120-cm2 tubing with 2 g progesterone) or Silastic intravaginal devices (SID) (120-cm2 tubing with 2 g progesterone) or a progesterone-releasing intravaginal device (PRID) (210-cm2 coil with 2 g progesterone). Blood samples were collected every 6 h on days −1, 4, 8, 12, 16 and 20 after insertion. Serum progesterone, quantitated by radioimmunoassay, varied with treatment (P < 0.01), with the day following treatment (P < 0.01) and with the time of day (P < 0.01). The highest concentrations (mean ±SE 2.1 ± 0.06 ng/mL) appeared at 0600 h and the lowest (mean ± SE 1.7 ± 0.08 ng/mL) at 1800 h. Serum progesterone was consistently greater (P < 0.01) in the PRID group. Location (subcutaneous vs. intravaginal) of the 120-cm2 tubing, i.e. double implant or SID, had no influence per se on serum progesterone. Heifers treated with the 60-cm2 tubing, i.e. single implant, had lower (P < 0.01) serum progesterone concentrations than heifers in the other three treatment groups. When the implants and devices were removed on day 20, two females in each of the four treatments received either saline, 5 mg estradiol-17β (E2), 500 IU PMSG, or 100 g GnRH. None of the heifers treated with the single implant ovulated. Of the saline-, E2-, PMSG- and GnRH-treated heifers, 0, 5, 6 and 2 showed estrus and 0, 2, 6 and 2 ovulated, respectively. PMSG-treated females had a mean of 2.5 ovulations. This study further demonstrates that adequate progesterone priming increases the response of prepuberal heifers to an ovulatory hormone stimulation. Key words: Progesterone, ovarian activity, prepuberal heifers, hormonal stimulation, silastic implants

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