Ceramide is a sphingolipid, which provides health benefits. Gas chromatography coupled
with flame ionized detector (GC-FID) was developed for targeted analysis of hydrolyzed
ceramide in color rice and by-products. Method validation was done by means of linearity,
repeatability and % recovery. R2
of 0.99 by means of linearity equation of the method was
obtained. The recovery was in the range of 69.85 – 108.73% with RSD of normalized
peak area lower than 10%. Hydrolyzed ceramide was found in unpolished rice, both
glutinous and non-glutinous rice and its by-products including, defatted rice bran, rice
bran wax and rice bran oil. The relationship between varieties of rice color and ceramide
content was classified using principal component analysis (PCA) into 2 groups, including
dark and pale color rice group. The highest levels of hydrolyzed ceramide as 21.11±0.02
mg/100 g was found in Mali Nil Surin (MNS), black non-glutinous rice. Whereas white
non-glutinous rice named Seebukantang (SBK) contained the lowest content of
hydrolyzed ceramide as 12.69±0.03 mg/100 g. The amount of ceramide in by-products
found in defatted rice bran, rice bran oil and rice bran wax were 17.43±0.38, 14.67±0.16
and 12.54±0.41 mg/100 g, respectively.
Comparative study on amount of nutraceuticals in by-products from solvent and cold pressing methods of rice bran oil processing