Long-term administration of Lactobacillus casei Zhang stabilized gut microbiota of adults and reduced gut microbiota age index of older adults

The gut microbiota plays an important role in intestinal health. Probiotics such as Lactobacillus are known to regulate gut microbes and prevent diseases. However, most of them are unable to colonize their stability in hosts’ intestinal tracts. In this study, we investigated the ability of Lactobacillus casei SY13 (SY13) to colonize the intestinal tract of BALB/c mice, after its oral administration for a short-term (once for a day) and long-term (once daily for 27 days) duration. Furthermore, we also evaluated the influence of its administration on the gut microbial structure and diversity in mice. Male BALB/c mice were gavaged with 108 colony-forming units (CFU) of SY13, and TaqMan-MGB probe and Illumina MiSeq sequencing were performed to assess the colonization ability and bacterial community structure in the cecum contents. The results showed that long-term treatment with SY13 enhanced its ability to form a colony in the intestine tract in contrast to the short-term treatment group, whose colony was retained for only 3 days. Oral administration of SY13 also significantly enhanced the gut microbial diversity. Short-term treatment with SY13 (SSY13) elevated Firmicutes and diminished Bacteroidetes phyla compared with long-term treatment (LSY13) and controls. The findings laid the foundation for the study of probiotic colonization ability and improvement of microbiota for the prevention of gut diseases.

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Lactobacillus casei Zhang exerts anti-obesity effect to obese glut1 and gut-specific-glut1 knockout mice via gut microbiota modulation mediated different metagenomic pathways

European Journal of Nutrition ◽

10.1007/s00394-021-02764-0 ◽

2022 ◽

Author(s):

Qiuwen He ◽

Yong Zhang ◽

Da Ma ◽

Weiqin Zhang ◽

Heping Zhang

Keyword(s):

Gut Microbiota ◽

Knockout Mice ◽

Lactobacillus Casei ◽

Lactobacillus Casei Zhang

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Curcumin Supplementation (Meriva®) Modulates Inflammation, Lipid Peroxidation and Gut Microbiota Composition in Chronic Kidney Disease

Nutrients ◽

10.3390/nu14010231 ◽

2022 ◽

Vol 14 (1) ◽

pp. 231

Author(s):

Francesca Pivari ◽

Alessandra Mingione ◽

Giada Piazzini ◽

Camilla Ceccarani ◽

Emerenziana Ottaviano ◽

...

Keyword(s):

Chronic Kidney Disease ◽

Lipid Peroxidation ◽

Kidney Disease ◽

Gut Microbiota ◽

Term Administration ◽

Inflammatory State ◽

Gut Microbiota Composition

Chronic kidney disease (CKD) subjects suffer from high risk of cardiovascular mortality, and any intervention preventing the progression of CKD may have an enormous impact on public health. In the last decade, there has been growing awareness that the gut microbiota (GM) can play a pivotal role in controlling the pathogenesis of systemic inflammatory state and CKD progression. To ameliorate the quality of life in CKD subjects, the use of dietary supplements has increased over time. Among those, curcumin has demonstrated significant in vitro anti-inflammatory properties. In this pilot study, 24 CKD patients and 20 healthy volunteers were recruited. CKD patients followed nutritional counselling and were supplemented with curcumin (Meriva®) for six months. Different parameters were evaluated at baseline and after 3–6 months: uremic toxins, metagenomic of GM, and nutritional, inflammatory, and oxidative status. Curcumin significantly reduced plasma pro-inflammatory mediators (CCL-2, IFN-γ, and IL-4) and lipid peroxidation. Regarding GM, after 6 months of curcumin supplementation, Escherichia-Shigella was significantly lower, while Lachnoclostridium was significant higher. Notably, at family level, Lactobacillaceae spp. were found significantly higher in the last 3 months of supplementation. No adverse events were observed in the supplemented group, confirming the good safety profile of curcumin phytosome after long-term administration.

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Gut microbiota and butyrate level changes associated with the long-term administration of proton pump inhibitors to old rats

Scientific Reports ◽

10.1038/s41598-019-43112-x ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 8

Author(s):

Sun Min Lee ◽

Nayoung Kim ◽

Ryoung Hee Nam ◽

Ji Hyun Park ◽

Soo In Choi ◽

...

Keyword(s):

Gut Microbiota ◽

Proton Pump Inhibitors ◽

Proton Pump ◽

Term Administration ◽

Old Rats

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Effects of Lactobacillus casei Shirota, Bifidobacterium breve, and oligofructose-enriched inulin on colonic nitrogen-protein metabolism in healthy humans

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00052.2006 ◽

2007 ◽

Vol 292 (1) ◽

pp. G358-G368 ◽

Cited By ~ 110

Author(s):

Vicky De Preter ◽

Tom Vanhoutte ◽

Geert Huys ◽

Jean Swings ◽

Luc De Vuyst ◽

...

Keyword(s):

Lactobacillus Casei ◽

Favorable Effect ◽

Rt Pcr ◽

N Content ◽

Bifidobacterium Breve ◽

Healthy Humans ◽

Lactobacillus Casei Shirota ◽

Term Administration ◽

Short And Long Term

Pre- and/or probiotics can cause changes in the ecological balance of intestinal microbiota and hence influence microbial metabolic activities. In the present study, the influence of oligofructose-enriched inulin (OF-IN), Lactobacillus casei Shirota, and Bifidobacterium breve Yakult on the colonic fate of NH3 and p-cresol was investigated. A randomized, placebo-controlled, crossover study was performed in 20 healthy volunteers to evaluate the influence of short- and long-term administration of OF-IN, L. casei Shirota, B. breve Yakult, and the synbiotic L. casei Shirota + OF-IN. The lactose[15N,15N]ureide biomarker was used to study the colonic fate of NH3. Urine and fecal samples were analyzed for 15N content by combustion-isotope ratio mass spectrometery and for p-cresol content by gas chromatography-mass spectrometry. RT-PCR was applied to determine the levels of total bifidobacteria. Both short- and long-term administration of OF-IN resulted in significantly decreased urinary p-cresol and 15N content. The reduction of urinary 15N excretion after short-term OF-IN intake was accompanied by a significant increase in the 15N content of the fecal bacterial fraction. However, this effect was not observed after long-term OF-IN intake. In addition, RT-PCR results indicated a significant increase in total fecal bifidobacteria after long-term OF-IN intake. Long-term L. casei Shirota and B. breve Yakult intake showed a tendency to decrease urinary 15N excretion, whereas a significant decrease was noted in p-cresol excretion. In conclusion, dietary addition of OF-IN, L. casei Shirota, and B. breve Yakult results in a favorable effect on colonic NH3 and p-cresol metabolism, which, in the case of OF-IN, was accompanied by an increase in total fecal bifidobacteria.

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The efficacy and safety of long-term administration of alpha-interferon and lamivudine in patients with chronic hepatitits B

Gastroenterology ◽

10.1016/s0016-5085(01)82843-2 ◽

2001 ◽

Vol 120 (5) ◽

pp. A572-A572

Author(s):

F JABOLI ◽

E RODA ◽

C FABBRI ◽

S MARCHETTO ◽

F FERRARA ◽

...

Keyword(s):

Alpha Interferon ◽

Efficacy And Safety ◽

Term Administration

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Author response for "The Effect Of Fall Biomechanics On Risk For Hip Fracture In Older Adults: A Cohort Study Of Video‐Captured Falls In Long‐Term Care"

10.1002/jbmr.4048/v2/response1 ◽

2020 ◽

Author(s):

Yijian Yang ◽

Vicki Komisar ◽

Nataliya Shishov ◽

Bryan Lo ◽

Alexandra MB Korall ◽

...

Keyword(s):

Older Adults ◽

Cohort Study ◽

Hip Fracture ◽

Long Term Care ◽

Author Response ◽

Term Care

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ALTERATION OF STEROID EXCRETION PATTERNS AFTER LONG-TERM ADMINISTRATION OF ORAL CONTRACEPTIVES TO HIRSUTE WOMEN

Acta Endocrinologica ◽

10.1530/acta.0.056s160 ◽

1967 ◽

Vol 56 (1_Suppl) ◽

pp. S160

Author(s):

Friedrich Husmann

Keyword(s):

Oral Contraceptives ◽

Term Administration ◽

Steroid Excretion

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STUDIES IN THE SUPPOSED GOITROGENOUS EFFECT OF TAP WATER IN HELSINKI

Acta Endocrinologica ◽

10.1530/acta.0.xxxiii0630 ◽

1960 ◽

Vol XXXIII (IV) ◽

pp. 630-636

Author(s):

F.-E. Krusius ◽

P. Peltola

Keyword(s):

Tap Water ◽

Physiological Saline ◽

Normal Weight ◽

The Body ◽

Iodine Uptake ◽

Redistilled Water ◽

Detectable Difference ◽

Term Administration ◽

Long Term Experiment

ABSTRACT The study reported here was performed in order to examine the tap water of Helsinki for its alleged goitrogenous effect. In a short-term, 24-hour experiment with rats, kept on an iodine-poor diet, we noticed no inhibition of the 4-hour 131I uptake, as compared with that of animals receiving physiological saline instead of tap water. Two similar groups of rats receiving 1 and 2 mg of mercazole in redistilled water showed a distinct blockage of the 4-hour uptake, which proved the effect of this substance. In a long-term experiment of 5 weeks' duration there was no detectable difference in the body weight, thyroid weight and the 4-hour 131I uptake when the rats receiving tap water or distilled water to which 0.45 per cent of sodium chloride was added were compared with each other. Replacement of tap water by a 10 mg per cent solution of mercazole in redistilled water enlarged the thyroid to double its normal weight and increased the 131I uptake to approximately five times that of the controls. Thus our experiments failed to demonstrate any goitrogenous effect in the tap water of Helsinki. Changes similar to those produced by a long-term administration of mercazole, i. e. an enlargement of the thyroid and an increased thyroidal iodine uptake, have been shown to be due to milk collected from goitrous areas. The observations here reported confirm the importance of milk in the genesis of the goitre endemia of Helsinki. Attention is further called to the fact that a thyroidal enlargement combined with an increased thyroidal iodine uptake cannot always be taken as a sign of iodine deficiency because similar changes may be produced by the administration of goitrogens.

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