scholarly journals Genetic characterisation of a complex class 1 integron in an NDM-1-producing Citrobacter freundii ST396 clinical strain isolated from a urine sample

2020 ◽  
Vol 23 ◽  
pp. 64-66
Author(s):  
Zhonghong Li ◽  
Yanfeng Lin ◽  
Lanfen Lu ◽  
Kaiying Wang ◽  
Lang Yang ◽  
...  
Keyword(s):  
Urine Sample ◽  
Clinical Strain ◽  
Citrobacter Freundii ◽  
Complex Class ◽  
Class 1 Integron ◽  
Genetic Characterisation ◽  
Class 1

Carriage of qnrA1 and qnrB2, blaCTX-M15, and complex class 1 integron in a clinical multiresistant Citrobacter freundii isolate

2010 ◽  
Vol 67 (2) ◽  
pp. 188-190 ◽  
Author(s):  
Sónia Ferreira ◽  
Ana Paradela ◽  
Jorge Velez ◽  
Elmano Ramalheira ◽  
Timothy R. Walsh ◽  
...  
Keyword(s):  
Citrobacter Freundii ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1

scholarly journals Complex Class 1 Integron in a Clinical Escherichia coli Strain From Vietnam Carrying Both mcr-1 and blaNDM–1

2019 ◽  
Vol 10 ◽  
Author(s):  
Hong-Ngoc Le-Vo ◽  
Phuong Thi-Bich Tran ◽  
Lien Le ◽  
Yuki Matsumoto ◽  
Daisuke Motooka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coli Strain ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1

scholarly journals Truncated tni Module Adjacent to the Complex Integron of Salmonella Genomic Island 1 in Salmonella enterica Serovar Virchow

2008 ◽  
Vol 53 (2) ◽  
pp. 824-827 ◽  
Author(s):  
Benoît Doublet ◽  
Chishih Chu ◽  
Cheng-Hsun Chiu ◽  
Yi-Chin Fan ◽  
Axel Cloeckaert
Keyword(s):  
Multidrug Resistance ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Open Reading Frame ◽  
Complex Class ◽  
Class 1 Integron ◽  
Reading Frame ◽  
Class 1 ◽  
First Time

ABSTRACT Salmonella genomic island 1 was identified for the first time in Salmonella enterica serovar Virchow isolated from humans in Taiwan. The complex class 1 integron conferring multidrug resistance was shown to be inserted within open reading frame (ORF) S023 and contains for the first time a partial transpositional module. The 5-bp target duplication flanking the complex integron suggests that its insertion in ORF S023 was by transposition.

scholarly journals qnrVC-Like Gene Located in a Novel Complex Class 1 Integron Harboring the ISCR1 Element in an Aeromonas punctata Strain from an Aquatic Environment in Shandong Province, China

2010 ◽  
Vol 54 (8) ◽  
pp. 3471-3474 ◽  
Author(s):  
Ruirui Xia ◽  
Xianhu Guo ◽  
Yuzhen Zhang ◽  
Hai Xu
Keyword(s):  
Amino Acid ◽  
Gene Cassette ◽  
Multidrug Resistant ◽  
Amino Acid Identity ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1 ◽  
Gyrase A ◽  
Ciprofloxacin Resistance ◽  
Novel Complex

ABSTRACT A qnrVC-like gene, qnrVC4, was found in a novel complex class 1 integron gene cassette array following the ISCR1 element and bla PER-1 in a multidrug-resistant strain of the aquatic bacterium Aeromonas punctata. The deduced QnrVC4 protein sequence shares 45% to 81% amino acid identity with quinolone resistance determinants QnrB6, QnrA1, QnrS1, QnrC, QnrVC1, and QnrVC3. A Ser-83 to Ile amino acid substitution in gyrase A may be mainly responsible for ciprofloxacin resistance in this strain.

scholarly journals Novel Variant (blaVIM-4) of the Metallo-β-Lactamase Gene blaVIM-1 in a Clinical Strain of Pseudomonas aeruginosa

2002 ◽  
Vol 46 (12) ◽  
pp. 4026-4028 ◽  
Author(s):  
Spyros Pournaras ◽  
Athanassios Tsakris ◽  
Maria Maniati ◽  
Leonidas S. Tzouvelekis ◽  
Antonios N. Maniatis
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Amino Acid ◽  
Amino Acid Position ◽  
Clinical Strain ◽  
Nucleotide Difference ◽  
Class 1 Integron ◽  
Class 1 ◽  
Novel Variant ◽  
Lactamase Gene

ABSTRACT A Pseudomonas aeruginosa isolate highly resistant to carbapenems was collected from a patient with postsurgical cerebrospinal infection in Greece. The isolate carried a class 1 integron that contained as a sole cassette the gene bla VIM-4, a novel variant of bla VIM-1, with one nucleotide difference resulting in a Ser-to-Arg change at amino acid position 175 of the VIM-1 enzyme. This is the first detection of a VIM-1 variant after its appearance in Italy.

scholarly journals Bioinformatic analysis of multi-drug resistant class 1 integron-coded protein of Citrobacter freundii

2021 ◽  
Vol 22 (3) ◽  
pp. 391-396
Author(s):  
O.D. Popoola ◽  
B.T. Thomas
Keyword(s):  
Secondary Structure ◽  
Alpha Helix ◽  
Random Coil ◽  
Pairwise Distance ◽  
Evolutionary Analysis ◽  
Citrobacter Freundii ◽  
Beta Turns ◽  
Class 1 Integron ◽  
Neutrality Test ◽  
Class 1

Background: The understanding of the secondary structure of the class 1 integron coded protein is necessary to decipher potential drug target and also to infer evolutionary ancestry at the proteomic level. This study was therefore aimed at determining the secondary structure of class 1 integron-coded protein and also to provide information on their evolutionary ancestry. Methodology: Five different sequences of Citrobacter freundii with the following accession numbers; KP902625.1, KP902624.1, KP902623.1, KP901093.1 and KP902609.1 were obtained using nucleotide BLAST (http://blast. ncbi.nlm.nih.gov/Blast.cgi) and subjected to evolutionary analysis, pairwise distance calculation, secondary structure and neutrality test using MEGA explorer, Kimura 2 parameter, SOPMA tool and Tajima’s test respectively. Results: Results of the NCBI queries revealed significant identity with class 1 integron of the studied Citrobacter freundii. The nucleotide sequence alignment depicted several conserved regions with varying degree of transitions, transversions, insertions, and deletions while the amino acid sequences of the nucleotides showed 42 conserved sites among all the sequences. The secondary structure of the class 1 integron coded protein depicted significant representation of the random coil (43.74±3.24), alpha helix (25.69±6.29) and the extended strands (22.42±2.41) than the beta turns (8.15±1.12). The Tajima’s Neutrality test of five nucleotide sequences of Citrobacter freundii analyzed by considering the first, second and third codons as well as the non-coding regions revealed a total of 127 positions in the final datasets while the Tajima’s Neutrality test was estimated to be -0.1038. Conclusion: The study confirmed common evolutionary ancestor for the class 1 integron coded protein found in Citrobacter freundii. Our study also documents the higher representation of random coil, alpha helix and extended strands than the beta turns. The negative value of the Tajima’s neutrality test suggests higher levels of both low and high frequency polymorphisms thus indicating a decrease in the class 1 integron population size and balancing selection Keywords: Evolutionary, Protein structure, Class 1 integrons, Citrobacter freundii

scholarly journals Complex Class 1 Integron CarryingqnrB62andblaVIM-2in a Citrobacter freundii Clinical Isolate

2016 ◽  
Vol 60 (11) ◽  
pp. 6937-6940 ◽  
Author(s):  
Jae Jin Lee ◽  
Mi-Na Kim ◽  
Kwang Seung Park ◽  
Jung Hun Lee ◽  
Asad Mustafa Karim ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Efflux Pump ◽  
Gene Array ◽  
Complex Class ◽  
Repeat Region ◽  
Inverted Repeat Region ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
Genetic Context

ABSTRACTThe coexistence ofqnrB62andblaVIM-2was detected in aCitrobacterclinical isolate. The reduced fluoroquinolone susceptibility is attributable toqnrB62, mutations of quinolone-resistance-determining regions, and an efflux pump or pumps. The genetic context surrounding chromosomalqnrB62was a novel complex class 1 integron (In1184::ISCR1::qnrB62) containing a unique gene array (blaVIM-2-aacA4′-8-gucD). An 18-nucleotide deletion at the 3′ end of thepspAgene [pspA(Δ18)], upstream ofqnrB62, and an inverted repeat region (IRR2) were detected in In1184::ISCR1::qnrB62, indicating past transposition events.

scholarly journals Molecular Characterization of Citrobacter freundii Isolated from Neonates in Neonatal Intensive Care Unit of Nepal

2012 ◽  
Vol 32 (2) ◽  
pp. 132-135 ◽  
Author(s):  
B Thapa ◽  
C Tribuddharat
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Neonatal Intensive Care ◽  
Infection Prevention And Control ◽  
Citrobacter Freundii ◽  
Class 1 Integron ◽  
Integrase Gene ◽  
Clonal Relatedness ◽  
Class 1

Introduction: Nosocomial Citrobacter spp. is emerging as a successful nosocomial pathogen in neonates in Nepal. The important risk factor being poor infection prevention and control practices. The objective of this study was to investigate the clonal relatedness of Citrobacter freundii isolated from clinical and nonclinical sources in Neonatal Intensive Care Unit (NICU) and to determine the presence of Extended Spectrum Beta-Lactamase (ESBL) genes and class 1 integron element. Materials and Methods: Polymerase chain Reaction (PCR) and PCR-Randomly Amplified Polymorphic DNA typing of the isolates were performed in three isolates to amplify class 1 integron element integrase gene, ESBL genes, and to study the clonal relatedness, respectively. Results: Two isolates harbored class 1 integron element. The blaCTX-M was present in all isolates and blaTEM-1 was present in one isolate. An isolate carried blaCTX-M and blaTEM-1 genes. All of these isolates were not clonally related. Conclusion: The study for the first time documented the emergence and spread of ESBL genes and class 1 integron element in multidrug resistant C. freundii in Nepal and urge for monitoring and surveillance of these strains. J. Nepal Paediatr. Soc. 32(2) 2012 132-135 doi: http://dx.doi.org/10.3126/jnps.v32i2.5804

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