Removal of total phenols from olive-mill wastewater using an agricultural by-product, olive pomace

The effect of acid cracking (pH 2.0; T 70 °C) and filtration as a pretreatment step on the chemical treatability of olive mill wastewater (chemical oxygen demand (COD) 150,000 m/L; total organic carbon (TOC) 36,000 mg/L; oil–grease 8,200 mg/L; total phenols 3,800 mg/L) was investigated. FeCl3 coagulation, Ca(OH)2 precipitation, electrocoagulation using stainless steel electrodes and the Fenton's reagent were applied as chemical treatment methods. Removal performances were examined in terms of COD, TOC, oil–grease, total phenols, colour, suspended solids and acute toxicity with the photobacterium Vibrio fischeri. Significant oil–grease (95%) and suspended solids (96%) accompanied with 58% COD, 43% TOC, 39% total phenols and 80% colour removals were obtained by acid cracking-filtration pretreatment. Among the investigated chemical treatment processes, electrocoagulation and the Fenton's reagent were found more effective after pretreatment, especially in terms of total phenols removal. Total phenols removal increased from 39 to 72% when pretreatment was applied, while no significant additional (≈10–15%) COD and TOC removals were obtained when acid cracking was coupled with chemical treatment. The acute toxicity of the original olive mill wastewater sample increased considerably after pretreatment from 75 to 89% (measured for the 10-fold diluted wastewater sample). An operating cost analysis was also performed for the selected chemical treatment processes.

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Effect of cell immobilization on the treatment of olive mill wastewater by a total phenols, acetic acid and formic acid degrading bacterium strain

Grasas y Aceites ◽

10.3989/gya.2005.v56.i2.118 ◽

2005 ◽

Vol 56 (2) ◽

Cited By ~ 4

Author(s):

Abdelghani El Asli ◽

Faouzi Errachidi ◽

Rhizlane Bennisse ◽

Abdel-illah Qatibi ◽

Mohamed Errami

Keyword(s):

Acetic Acid ◽

Formic Acid ◽

Cell Immobilization ◽

Olive Mill Wastewater ◽

Total Phenols ◽

Degrading Bacterium ◽

Bacterium Strain ◽

Olive Mill

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Hydrothermal Carbonization of Olive Pomace Using Olive Mill Wastewater as a Conversion Media

Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions (2nd Edition) - Environmental Science and Engineering ◽

10.1007/978-3-030-51210-1_28 ◽

2021 ◽

pp. 165-169

Author(s):

Ahmed Amine Azzaz ◽

Mejdi Jeguirim ◽

Camélia Ghimbeu ◽

Simona Bennici ◽

Lionel Limousy ◽

...

Keyword(s):

Hydrothermal Carbonization ◽

Olive Mill Wastewater ◽

Olive Pomace ◽

Olive Mill

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Microbial Application to Improve Olive Mill Wastewater Phenolic Extracts

Molecules ◽

10.3390/molecules26071944 ◽

2021 ◽

Vol 26 (7) ◽

pp. 1944

Author(s):

Flora V. Romeo ◽

Gina Granuzzo ◽

Paola Foti ◽

Gabriele Ballistreri ◽

Cinzia Caggia ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Olive Mill Wastewater ◽

Health Claims ◽

Total Phenols ◽

Wickerhamomyces Anomalus ◽

Phenolic Extract ◽

Beta Glucosidase ◽

Commercial Adsorbent ◽

Olive Mill ◽

Phenolic Extracts

Olive mill wastewater (OMW) contains valuable and interesting bioactive compounds, among which is hydroxytyrosol, which is characterized by a remarkable antioxidant activity. Due to the health claims related to olive polyphenols, the aim of this study was to obtain an extract from OMW with an increased level of hydroxytyrosol by means of microbial enzymatic activity. For this purpose, four commercial adsorbent resins were selected and tested. The beta-glucosidase and esterase activity of strains of Wickerhamomyces anomalus, Lactiplantibacillus plantarum, and Saccharomyces cerevisiae were also investigated and compared to those of a commercial enzyme and an Aspergillus niger strain. The W. anomalus strain showed the best enzymatic performances. The SP207 resin showed the best efficiency in selective recovery of hydroxytyrosol, tyrosol, oleuropein, and total phenols. The bioconversion test of the OMW extract was assessed by using both culture broths and pellets of the tested strains. The results demonstrated that the pellets of W. anomalus and L. plantarum were the most effective in hydroxytyrosol increasing in phenolic extract. The interesting results suggest the possibility to study new formulations of OMW phenolic extracts with multifunctional microorganisms.

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Screening of Commercial Catalysts for Steam Reforming of Olive Mill Wastewater

Renewable Energy ◽

10.1016/j.renene.2020.12.139 ◽

2021 ◽

Author(s):

Cláudio Rocha ◽

M.A. Soria ◽

Luís M. Madeira

Keyword(s):

Steam Reforming ◽

Olive Mill Wastewater ◽

Olive Mill

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Technoeconomic Analysis of the Recovery of Phenols from Olive Mill Wastewater through Membrane Filtration and Resin Adsorption/Desorption

Sustainability ◽

10.3390/su13042376 ◽

2021 ◽

Vol 13 (4) ◽

pp. 2376

Author(s):

Dimitris P. Zagklis ◽

Costas S. Papageorgiou ◽

Christakis A. Paraskeva

Keyword(s):

Capital Investment ◽

Membrane Filtration ◽

Hot Spot ◽

Treatment Method ◽

Olive Mill Wastewater ◽

Operational Cost ◽

Equipment Cost ◽

Fixed Capital Investment ◽

Adsorption Desorption ◽

Olive Mill

Olive mill wastewater is an important agro-industrial waste with no established treatment method. The authors have developed a phenol separation method that could potentially cover the treatment cost of the waste. The purpose of this study was to identify any economic hotspots in the process, the operational cost and examine the margin of profit for such a process. The equipment cost was scaled for different treatment capacities and then used to estimate the fixed capital investment and the yearly operational cost. The highest purchased equipment cost was identified for the membrane filtration system, while the cost for resin replacement was identified as the highest operational cost. The lifespan of the resin used in the adsorption step was identified as an economic hot spot for the process, with the phenols separation cost ranging from 0.84 to 13.6 €/g of phenols for a resin lifespan of 5–100 adsorption/desorption cycles. The lifespan of the resin proved to be the single most important aspect that determines the phenols separation cost. The price range that was calculated for the product of the process is very promising because of the typical value of antioxidants and the low concentration of phenols that are needed for food supplements and cosmetics.

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Biocomposite spheres based on aluminum oxide dispersed with orange-peel powder for adsorption of phenol from batch membrane fraction of olive mill wastewater

Colloids and Interface Science Communications ◽

10.1016/j.colcom.2021.100402 ◽

2021 ◽

Vol 42 ◽

pp. 100402

Author(s):

Jacques Romain Njimou ◽

John Godwin ◽

Hugues Pahimi ◽

S. Andrada Maicaneanu ◽

Fridolin Kouatchie-Njeutcha ◽

...

Keyword(s):

Aluminum Oxide ◽

Membrane Fraction ◽

Orange Peel ◽

Olive Mill Wastewater ◽

Olive Mill

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Antioxidant Properties of Olive Mill Wastewater Polyphenolic Extracts on Human Endothelial and Vascular Smooth Muscle Cells

Foods ◽

10.3390/foods10040800 ◽

2021 ◽

Vol 10 (4) ◽

pp. 800

Author(s):

Anna Maria Posadino ◽

Annalisa Cossu ◽

Roberta Giordo ◽

Amalia Piscopo ◽

Wael M Abdel-Rahman ◽

...

Keyword(s):

Antioxidant Activity ◽

Cell Death ◽

Smooth Muscle ◽

Vascular Smooth Muscle ◽

Smooth Muscle Cells ◽

Olive Oil ◽

Vascular Smooth Muscle Cells ◽

Muscle Cells ◽

Olive Mill Wastewater ◽

Olive Mill

This work aims to analyze the chemical and biological evaluation of two extracts obtained by olive mill wastewater (OMW), an olive oil processing byproduct. The exploitation of OMW is becoming an important aspect of development of the sustainable olive oil industry. Here we chemically and biologically evaluated one liquid (L) and one solid (S) extract obtained by liquid–liquid extraction followed by acidic hydrolysis (LLAC). Chemical characterization of the two extracts indicated that S has higher phenol content than L. Hydroxytyrosol and tyrosol were the more abundant phenols in both OMW extracts, with hydroxytyrosol significantly higher in S as compared to L. Both extracts failed to induce cell death when challenged with endothelial cells and vascular smooth muscle cells in cell viability experiments. On the contrary, the higher extract dosages employed significantly affected cell metabolic activity, as indicated by the MTT tests. Their ability to counteract H2O2-induced oxidative stress and cell death was assessed to investigate potential antioxidant activities of the extracts. Fluorescence measurements obtained with the reactive oxygen species (ROS) probe H2DCF-DA indicated strong antioxidant activity of the two OMW extracts in both cell models, as indicated by the inhibition of H2O2-induced ROS generation and the counteraction of the oxidative-induced cell death. Our results indicate LLAC-obtained OMW extracts as a safe and useful source of valuable compounds harboring antioxidant activity.

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