scholarly journals Antioxidant Properties of Olive Mill Wastewater Polyphenolic Extracts on Human Endothelial and Vascular Smooth Muscle Cells

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 800
Author(s):  
Anna Maria Posadino ◽  
Annalisa Cossu ◽  
Roberta Giordo ◽  
Amalia Piscopo ◽  
Wael M Abdel-Rahman ◽  
...  

This work aims to analyze the chemical and biological evaluation of two extracts obtained by olive mill wastewater (OMW), an olive oil processing byproduct. The exploitation of OMW is becoming an important aspect of development of the sustainable olive oil industry. Here we chemically and biologically evaluated one liquid (L) and one solid (S) extract obtained by liquid–liquid extraction followed by acidic hydrolysis (LLAC). Chemical characterization of the two extracts indicated that S has higher phenol content than L. Hydroxytyrosol and tyrosol were the more abundant phenols in both OMW extracts, with hydroxytyrosol significantly higher in S as compared to L. Both extracts failed to induce cell death when challenged with endothelial cells and vascular smooth muscle cells in cell viability experiments. On the contrary, the higher extract dosages employed significantly affected cell metabolic activity, as indicated by the MTT tests. Their ability to counteract H2O2-induced oxidative stress and cell death was assessed to investigate potential antioxidant activities of the extracts. Fluorescence measurements obtained with the reactive oxygen species (ROS) probe H2DCF-DA indicated strong antioxidant activity of the two OMW extracts in both cell models, as indicated by the inhibition of H2O2-induced ROS generation and the counteraction of the oxidative-induced cell death. Our results indicate LLAC-obtained OMW extracts as a safe and useful source of valuable compounds harboring antioxidant activity.

2008 ◽  
Vol 103 (5) ◽  
Author(s):  
Alexandra E. Ewence ◽  
Martin Bootman ◽  
H. Llewelyn Roderick ◽  
Jeremy N. Skepper ◽  
Geraldine McCarthy ◽  
...  

2001 ◽  
Vol 280 (3) ◽  
pp. C709-C718 ◽  
Author(s):  
Tzong-Shyuan Lee ◽  
Lee-Young Chau

Oxidized low-density lipoprotein (oxLDL) is a potent inducer of apoptosis for vascular cells. In the present study, we demonstrate that the expression of death mediators, including p53, Fas, and Fas ligand (FasL) was substantially upregulated by oxLDL in cultured vascular smooth muscle cells (SMCs). The induction of these death mediators was time dependent and was accompanied by an increase in apoptotic death of SMCs following oxLDL treatment. Two oxysterols, 7β-hydroxycholesterol and 25-hydroxycholesterol, were also effective to induce the expression of death mediators and apoptosis. α-Tocopherol and deferoxamine significantly attenuated the induction of death mediators and cell death induced by oxLDL and oxysterols, suggesting that reactive oxygen species are involved in triggering the apoptotic event. Incubation of cells with FasL-neutralizing antibody inhibited the oxLDL-induced cell death up to 50%. Furthermore, caspase 8 and caspase 3 activities were induced time dependently in SMCs following oxLDL treatment. Collectively, these data suggest that the Fas/FasL death pathway is activated and responsible for, at least in part, the apoptotic death in vascular SMCs upon exposure to oxLDL.


1998 ◽  
Vol 31 ◽  
pp. S351-S353 ◽  
Author(s):  
Takeshi Nakahashi ◽  
Keisuke Fukuo ◽  
Hiroyuki Nishimaki ◽  
Shigeki Hata ◽  
Masumi Shimizu ◽  
...  

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jing Zhao ◽  
Yuhei Nishimura ◽  
Akihiko Kimura ◽  
Kentaro Ozawa ◽  
Toshikazu Kondo ◽  
...  

2012 ◽  
Vol 18 (38) ◽  
pp. 6331-6338 ◽  
Author(s):  
Arturo Giordano ◽  
Simona Romano ◽  
Giovanna Nappo ◽  
Stefano Messina ◽  
Michele Polimeno ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document