Biologically synthesized silver nanoparticles enhances antibiotic activity against Gram-negative bacteria

2015 ◽  
Vol 29 ◽  
pp. 217-226 ◽  
Author(s):  
Sangiliyandi Gurunathan
Keyword(s):  
Silver Nanoparticles ◽  
Antibiotic Activity ◽  
Gram Negative Bacteria ◽  
Gram Negative

scholarly journals Biogenic Silver Nanoparticles Conjugated with Nisin: Improving the Antimicrobial and Antibiofilm Properties of Nanomaterials

Chemistry ◽  
2021 ◽  
Vol 3 (4) ◽  
pp. 1271-1285
Author(s):  
Patricia Zimet ◽  
Ruby Valadez ◽  
Sofía Raffaelli ◽  
María Belén Estevez ◽  
Helena Pardo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Properties ◽  
Value Added ◽  
Green Technology ◽  
Natural Food ◽  
Antibiofilm Activity ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Biogenic Silver Nanoparticles

Microbial technology offers a green alternative for the synthesis of value-added nanomaterials. In particular, fungal compounds can improve silver nanoparticle production, stabilizing colloidal nanoparticles. Based on a previous study by our group, silver nanoparticles obtained using the extracellular cell-free extracts of Phanerochaete chrysosporium (PchNPs) have shown antimicrobial and antibiofilm activity against Gram-negative bacteria. Moreover, nisin—a bacteriocin widely used as a natural food preservative—has recently gained much attention due its antimicrobial action against Gram-positive bacteria in biomedical applications. Therefore, the aim of this work was to conjugate biogenic silver nanoparticles (PchNPs) with nisin to obtain nanoconjugates (PchNPs@nis) with enhanced antimicrobial properties. Characterization assays were conducted to determine physicochemical properties of PchNPs@nis, and also their antibacterial and antibiofilm activities were studied. The formation of PchNPs@nis was confirmed by UV-Vis, TEM, and Raman spectroscopy analysis. Different PchNPs@nis nanobioconjugates showed diameter values in the range of 60–130 nm by DLS and surface charge values between −20 and −13 mV. Nisin showed an excellent affinity to PchNPs, with binding efficiencies higher than 75%. Stable synthesized PchNPs@nis nanobioconjugates were not only able to inhibit biofilm formation by S. aureus, but also showed inhibition of the planktonic cell growth of Staphyloccocus aureus and Escherichia coli, broadening the spectrum of action of the unconjugated antimicrobials against Gram-positive and Gram-negative bacteria. In conclusion, these results show the promising application of PchNPs@nis, prepared via green technology, as potential antimicrobial nanomaterials.

scholarly journals Bactericidal Silver Nanoparticles by Atmospheric Pressure Solution Plasma Processing

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 874 ◽  
Author(s):  
Janith Weerasinghe ◽  
Wenshao Li ◽  
Rusen Zhou ◽  
Renwu Zhou ◽  
Alexander Gissibl ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Atmospheric Pressure ◽  
Chemical Reduction ◽  
Cost Effective ◽  
Pressure Solution ◽  
Gram Negative Bacteria ◽  
Promising Alternative ◽  
Gram Negative ◽  
Transmission Electron ◽  
Solution Plasma Processing

Silver nanoparticles have applications in plasmonics, medicine, catalysis and electronics. We report a simple, cost-effective, facile and reproducible technique to synthesise silver nanoparticles via plasma-induced non-equilibrium liquid chemistry with the absence of a chemical reducing agent. Silver nanoparticles with tuneable sizes from 5.4 to 17.8 nm are synthesised and characterised using Transmission Electron Microscopy (TEM) and other analytic techniques. A mechanism for silver nanoparticle formation is also proposed. The antibacterial activity of the silver nanoparticles was investigated with gram-positive and gram-negative bacteria. The inhibition of both bacteria types was observed. This is a promising alternative method for the instant synthesis of silver nanoparticles, instead of the conventional chemical reduction route, for numerous applications.

scholarly journals A Study on Antibacterial Activity of Silver Nanoparticles

2018 ◽  
Vol 29 (2) ◽  
pp. 37-41
Author(s):  
M Iqbal Hossain ◽  
M Anwar Habib ◽  
Nazimuddin Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Reference Drug ◽  
Gram Negative ◽  
Quasi Experimental

This quasi experimental study was designed to evaluate antibacterial activity of chemically synthesized silver nanoparticles (AgNPs) from silver nitrate (AgNO3) solutions on gram negative bacteria like E.Coli using disc diffusion method. Different concentrations of AgNPs, AgNO3 and reference drug ciprofloxacin were used to find out the antibacterial activity which revealed that AgNPs possessed significant antibacterial effect compared to AgNO3 solutions but relatively less antibacterial effect than that of ciprofloxacin. So chemical synthesis guided AgNPs may have some antibacterial effects.TAJ 2016; 29(2): 37-41

Comparative Analysis of the Activity of Silver Nanoparticles against Native Microflora from Poultry Processing Plants Wastes

2017 ◽  
Vol 13 ◽  
pp. 176-182
Author(s):  
Anastasia I. Piskaeva ◽  
Yuriy Yu. Sidorin ◽  
Alexander Yu. Prosekov
Keyword(s):  
Silver Nanoparticles ◽  
Model Test ◽  
Gram Negative Bacteria ◽  
Lactobacillus Salivarius ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Bactericidal Properties ◽  
Processing Plants ◽  
Poultry Processing ◽  
Native Microflora

The aim of this study was to examine and compare bactericidal properties of silver nanoparticles on strains isolated from poultry processing plants wastes. Strains Еscherichia coli В-6240, Salmonella typhimurium В3533 were used as a model test strains for Gram-negative bacteria and Lactobacillus sp. B3730, Lactobacillus salivarius B5001, Thermoactinomyces thalpophilus AC936 were used as a model test strains for Gram-positive bacteria.

scholarly journals Green Synthesis of Silver Nanoparticles Using Novel Launaea Procumbens Leaves Extract and Screening Its Antibacterial Activity

2021 ◽  
Author(s):  
Sanjay Ratan Kumavat ◽  
SATYENDRA MISHRA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Launaea Procumbens

Abstract Plants are emerging as a cost-effective and ecofriendly method for green synthesis of nanoparticles. The plant extract Launaea procumbens was used as a reduction agent in the green synthesis of silver nanoparticles. UV-Visible spectroscopy, HR-TEM, SAED, FE-SEM, EDAX, DLS, and FT-IR were used to study the green synthesized silver nanoparticles. UV-Vis spectroscopy of a prepared silver solution revealed maximum absorption at 435 nm. The synthesized silver nanoparticles were found to be spherical in shape with a size in the range of 24.28 to 31.54 nm. DLS analysis was used to determine the size of the green synthesized silver nanoparticles, which showed outstanding antibacterial action against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, as well as Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. Gram-positive Bacillus subtilis had a maximum zone of inhibition of 20 mm, Staphylococcus aureus had a zone of inhibition of 19 mm, and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa had zones of inhibition of 13 mm.

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