Characterisation of growth plate dynamics in surgical and non-invasive loaded murine models of osteoarthritis

2021 ◽  
Vol 29 ◽  
pp. S12
Author(s):  
H.J. Samvelyan ◽  
K. Madi ◽  
A.E. Törnqvist ◽  
B. Javaheri ◽  
K.A. Staines
Keyword(s):  
Growth Plate ◽  
Murine Models ◽  
Non Invasive ◽  
Plate Dynamics

scholarly journals Characterisation of growth plate dynamics in murine models of osteoarthritis

2020 ◽  
Author(s):  
Hasmik J. Samvelyan ◽  
Kamel Madi ◽  
Anna E. Törnqvist ◽  
Behzad Javaheri ◽  
Katherine A. Staines
Keyword(s):  
Articular Cartilage ◽  
Growth Plate ◽  
Volume Fraction ◽  
Left Knee ◽  
Bone Volume ◽  
Knee Joints ◽  
Murine Models ◽  
Male Mice ◽  
Bone Volume Fraction ◽  
Plate Dynamics

AbstractBackgroundThe purpose of this study was to investigate growth plate dynamics in surgical and loading murine models of osteoarthritis, to understand whether abnormalities in these dynamics predict those at risk of osteoarthritis.Methods8-week-old C57BL/6 male mice underwent destabilisation of medial meniscus (DMM) (n = 8) surgery in right knee joints. Contralateral left knee joints had no intervention (controls). In 16-week-old C57BL/6 male mice (n = 6), osteoarthritis was induced using non-invasive mechanical loading of right knee joints with peak force of 11N. Non-loaded left knee joints were internal controls. Chondrocyte transiency in tibial articular cartilage and growth plate was examined by histology and immunohistochemistry. Tibial subchondral bone parameters were measured using microCT and correlated to GP bridging.ResultsHigher expression of chondrocyte hypertrophy markers; Col10a1 and MMP13 were observed in tibial articular cartilage chondrocytes of DMM and loaded mice. In tibial growth plate, Col10a1 and MMP13 expressions were widely dispersed in a significantly enlarged zone of proliferative and hypertrophic chondrocytes. 3-dimensional quantification revealed enriched growth plate bridging and higher bridge densities in medial compared to lateral tibiae of DMM and loaded knee joints of the mice. Growth plate dynamics were associated with increased subchondral bone volume fraction (BV/TV; %) in medial tibiae of DMM and loaded knee joints and epiphyseal trabecular bone volume fraction in medial tibiae of loaded knee joints.ConclusionsThe results confirm associations between aberrant chondrocyte hypertrophy marker expression and osteoarthritis pathology in a surgical and loaded murine model of osteoarthritis. Spatial variations in growth plate bridging formation revealed accelerated cartilage-bone transitions which may contribute to anatomical variation in vulnerability to osteoarthritis development in these models.

scholarly journals Thermographic Screening of Beef Cattle Metatarsal Growth Plate Lesions

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 191
Author(s):  
Giorgia Fabbri ◽  
Matteo Gianesella ◽  
Rossella Tessari ◽  
Andrea Bassini ◽  
Massimo Morgante ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Growth Plate ◽  
Imaging Techniques ◽  
Infrared Camera ◽  
Correct Treatment ◽  
Non Invasive ◽  
Hind Limbs ◽  
Practical Tool ◽  
Beef Bulls ◽  
Maximum Temperatures

Lameness represents one of the main causes of decreased productive performance and impaired animal welfare in the bovine industry. Young beef bulls are predisposed to develop diseases of the growing skeleton, especially growth plate lesions. Early diagnosis is indispensable for ensuring correct treatment, fast recovery and reduction losses. However, when dealing with beef cattle, this is not always possible. Fast and reliable diagnostic imaging techniques are necessary to improve dealing with lameness in beef animals. The aim of the present study was to examine the potential of thermographic imaging as a non-invasive tool for rapidly screening beef bulls for the presence of growth plate lesions. Here, 20 Charolais and Limousine beef bulls affected by growth plate lesions in one of the rear limbs were selected. IRT was performed on both hind limbs using a digital infrared camera (ThermaCam T420 Model, Flir Systems, Boston, MA, USA), prior to radiographic imaging and clinical examination. The temperature of healthy and affected limbs was measured in two regions: the area correspondent to the growth plate (AR01) and the whole area of the metatarsus (AR02). Growth plate lesions were found to increase the maximum, mean, and minimum temperatures in AR01; and the mean and maximum temperatures in AR02, therefore, indicating the potential of IRT as a reliable, practical tool for screening growth plate lesions in beef bulls.

scholarly journals FSTL3: a novel regulator of growth plate dynamics?

2014 ◽  
Vol 22 ◽  
pp. S347
Author(s):  
A. Burleigh ◽  
K.A. Staines ◽  
B. Poulet ◽  
A.A. Pitsillides ◽  
A. Mukherjee
Keyword(s):  
Growth Plate ◽  
Plate Dynamics

Use of non-invasive imaging to monitor response to aflibercept treatment in murine models of colorectal cancer liver metastases

2016 ◽  
Vol 34 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Karianne G. Fleten ◽  
Kine M. Bakke ◽  
Gunhild M. Mælandsmo ◽  
Andreas Abildgaard ◽  
Kathrine Røe Redalen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Liver Metastases ◽  
Murine Models ◽  
Colorectal Cancer Liver Metastases ◽  
Non Invasive

scholarly journals Associations between chondrocyte transiency, bone growth and osteoarthritis pathology in surgical and non-invasive loaded murine models

Bone Reports ◽  
2021 ◽  
Vol 14 ◽  
pp. 100843
Author(s):  
Hasmik Jasmine Samvelyan ◽  
Kamel Madi ◽  
Anna E. Törnqvist ◽  
Behzad Javaheri ◽  
Katherine Staines
Keyword(s):  
Bone Growth ◽  
Murine Models ◽  
Non Invasive

X-ray microtomography

1988 ◽  
Vol 46 ◽  
pp. 998-999
Author(s):  
H.W. Deckman ◽  
B.F. Flannery ◽  
J.H. Dunsmuir ◽  
K.D' Amico
Keyword(s):  
Computed Tomography ◽  
Internal Structure ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Tissue Structure ◽  
Individual Element ◽  
X Ray ◽  
Non Invasive ◽  
Panoramic Imaging ◽  
Three Dimensional Images

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.

Effects of Hypophysectomy on the Occurrence and Calcifiability of Matrix Vesicles in the Epiphyseal Growth Plate of Immature Rats

1980 ◽  
Vol 38 ◽  
pp. 578-579
Author(s):  
S. I. Coleman ◽  
W. J. Dougherty
Keyword(s):  
Growth Plate ◽  
Matrix Vesicles ◽  
Epiphyseal Growth Plate ◽  
Hard Tissue ◽  
Hormonal Factors ◽  
Post Surgery ◽  
Mineral Deposition ◽  
Thin Sectioning ◽  
Integral Role ◽  
Cellular Secretion

In the cellular secretion theory of mineral deposition, extracellular matrix vesicles are believed to play an integral role in hard tissue mineralization (1). Membrane limited matrix vesicles arise from the plasma membrane of epiphyseal chondrocytes and tooth odontoblasts by a budding process (2, 3). Nutritional and hormonal factors have been postulated to play essential roles in mineral deposition and apparently have a direct effect on matrix vesicles of calcifying cartilage as concluded by Anderson and Sajdera (4). Immature (75-85 gm) Long-Evans hooded rats were hypophysectomized by the parapharyngeal approach and maintained fourteen (14) days post-surgery. At this time, the animals were anesthetized and perfusion fixed in cacodylate buffered 2.5% glutaraldehyde. The proximal tibias were quickly dissected out and split sagittally. One half was used for light microscopy (LM) and the other for electron microscopy (EM). The halves used for EM were cut into blocks approximately 1×3 mm. The tissue blocks were prepared for ultra-thin sectioning and transmission EM. The tissue was oriented so as to section through the epiphyseal growth plate from the zone of proliferating cartilage on down through the hypertrophic zone and into the initial trabecular bone. Sections were studied stained (double heavy metal) and unstained.

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