Decellularized human fetal intestine as a bioscaffold for regeneration of the rabbit bladder submucosa

It has been reported that explants of human fetal intestine can be maintained in culture for up to 21 days in a viable condition and that these organ cultures support the growth of a variety of known viral agents responsible for enteric disease. Scanning electron microscopy (SEM) has been undertaken on several series of these explants to determine their appearance under routine culture conditions.Fresh specimens of jejunum obtained from normal human fetuses were washed, dissected into l-4mm pieces, and cultured in modified Leibowitz L-15 medium at 34° C as previously described. Serial specimens were fixed each day in 3% glutaraldehyde for 90 minutes at room temperature, rinsed, dehydrated, and dried by the CO2 critical point method in a Denton DCP-1 device. Specimens were attached to aluminum stubs with 3M transfer tape No. 465, and one sample on each stub was carefully rolled along the adhesive such that villi were broken off to expose their interiors.

Download Full-text

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141743 ◽

1995 ◽

Vol 53 ◽

pp. 1074-1075

Author(s):

F.E. Hossler ◽

M.I. McKamey ◽

F.C. Monson

Keyword(s):

Lymph Nodes ◽

Light Microscopy ◽

Corrosion Casting ◽

Fixed Tissue ◽

Infusion Pressure ◽

India Ink ◽

Cacodylate Buffer ◽

Rabbit Bladder ◽

Lateral Walls ◽

Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

Download Full-text

Tetrodotoxin-resistant release of ATP from superfused rabbit detrusor muscle during electrical field stimulation in the presence of luciferin–luciferase

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y84-026 ◽

1984 ◽

Vol 62 (1) ◽

pp. 153-156 ◽

Cited By ~ 15

Author(s):

Archana Chaudhry ◽

John W. Downie ◽

Thomas D. White

Keyword(s):

Action Potentials ◽

Electrical Field Stimulation ◽

Detrusor Muscle ◽

Electrical Field ◽

Stimulation Parameters ◽

Rabbit Bladder ◽

Firefly Luciferin ◽

Rabbit Urinary Bladder ◽

Stimulation Of

The present study was carried out to assess the possible role of ATP in the noncholinergic, nonadrenergic transmission in the rabbit urinary bladder. When rabbit detrusor muscle strips were superfused with medium containing firefly luciferin–luciferase and stimulated transmurally at low stimulation parameters, tetrodotoxin-sensitive contractions were obtained but no release of ATP could be detected. However, at somewhat higher stimulation parameters, release of ATP was observed. This release of ATP was not diminished by tetrodotoxin indicating that ATP was not likely released as a result of propagated action potentials in nerves. Because contractions persisted in the presence of tetrodotoxin, it is possible that the ATP might have been released as a result of direct electrical stimulation of the muscle. These results do not support the idea that ATP is released as a neurotransmitter in the rabbit bladder.

Download Full-text

Rapid injection cystometry and hydroxyproline content in the acutely overdistended rabbit bladder

Neurourology and Urodynamics ◽

10.1002/nau.1930110104 ◽

1992 ◽

Vol 11 (1) ◽

pp. 33-39

Author(s):

Hayoung Kim ◽

William D. Belville ◽

Gary Wedemeyer ◽

Edward J. McGuire

Keyword(s):

Hydroxyproline Content ◽

Rapid Injection ◽

Rabbit Bladder

Download Full-text

Human Milk as an Enteral Source of Granulocyte Colony-Stimulating Factor (G-CSF): 1) Concentrations in Preterm and Term Milk, 2) G-CSF Survival after In Vitro Simulations of Digestion, and 3) Expression of G-CSF Receptor (G-CSF-R) in Human Fetal Intestine

Pediatric Research ◽

10.1203/00006450-199904020-01114 ◽

1999 ◽

Vol 45 (4, Part 2 of 2) ◽

pp. 187A-187A

Author(s):

Darlene A Calhoun ◽

Mathilde Lunoe ◽

Yan Du ◽

Robert D Christensen

Keyword(s):

Human Milk ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Stimulating Factor ◽

Fetal Intestine ◽

Factor G

Download Full-text

Effects of Shielded or Unshielded Laser and Electrohydraulic Lithotripsy on Rabbit Bladder

The Journal of Urology ◽

10.1016/s0022-5347(17)40117-0 ◽

1990 ◽

Vol 143 (4) ◽

pp. 857-860 ◽

Cited By ~ 28

Author(s):

Krishna M. Bhatta ◽

David I. Rosen ◽

Thomas J. Flotte ◽

Stephen P. Dretler ◽

Norman S. Nishioka

Keyword(s):

Electrohydraulic Lithotripsy ◽

Rabbit Bladder

Download Full-text

Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis

ISRN Urology ◽

10.5402/2012/618247 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5

Author(s):

Brittany Fitzpatrick ◽

Catherine Schuler ◽

Robert E. Leggett ◽

Robert M. Levin

Keyword(s):

Quantitative Analysis ◽

Western Blotting ◽

Optical Density ◽

Tissue Concentration ◽

Detrusor Muscle ◽

Pvdf Membrane ◽

Sds Page ◽

Wash Buffer ◽

Rabbit Bladder ◽

Plate Analysis

Purpose. Nitrotyrosine was quantitated in rabbit bladder muscle and mucosa using two analytical systems: Western blotting analyses and a 96-well plate quantitative analysis kit. Materials and Methods. Rabbit bladder muscle and mucosa were obtained from control rabbits. For the Western analysis, the samples were loaded into a SDS page gel and then transferred to a PVDF membrane. The optical density was measured using a Kodak Scanner. Using the 96-well plate, the samples and standards were loaded, incubated with primary and secondary antibody, washed and vacuumed with 10x wash buffer three times between each incubation period. Stop buffer was added to the plate and the results were quantified via the plate reader. Results. For both muscle and mucosa tissue, the optical density readings were linear with tissue concentration; the concentration of nitrotyrosine in the mucosa was significantly higher than in the muscle. However, whereas the Western blot analysis is based on relative optical densities, the 96-well plate kit provides a truly quantitative analysis. Discussion. Mucosa tissue displayed a higher density of nitrotyrosine than did detrusor muscle tissue. This may well be due to the significantly higher metabolic activity of the mucosa compared to the muscle.

Download Full-text