Spectroscopic assessment of endogenous porphyrins in a rheumatoid arthritis rabbit model after the application of ALA and ALA-Me

2013 ◽  
Vol 119 ◽  
pp. 15-21 ◽  
Author(s):  
R. Rudys ◽  
G. Kirdaitė ◽  
S. Bagdonas ◽  
L. Leonavičienė ◽  
R. Bradūnaitė ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rabbit Model

scholarly journals Moxibustion of Zusanli (ST36) and Shenshu (BL23) Alleviates Cartilage Degradation through RANKL/OPG Signaling in a Rabbit Model of Rheumatoid Arthritis

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Yang Chen ◽  
Haijun Li ◽  
Xiaochao Luo ◽  
Huahui Liu ◽  
Yumei Zhong ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Inflammatory Responses ◽  
Musculoskeletal Diseases ◽  
Alternative Therapy ◽  
Rabbit Model ◽  
Bone Destruction ◽  
Cartilage Degradation ◽  
Protective Effects ◽  
Rankl Mrna ◽  
Autoimmune Inflammatory Disease

Rheumatoid arthritis (RA) is a systemic and chronic autoimmune inflammatory disease characterized by severe synovial hyperplasia associated with progressive cartilage degradation. Due to the severe pain and disability caused by RA, effective therapeutic strategies that could simultaneously alleviate the inflammatory response and delay the disease progression are urgently needed. As a major alternative therapy in traditional Chinese medicine, moxibustion has been demonstrated that it could reduce the chronic inflammatory responses of a series of musculoskeletal diseases; however, whether moxibustion has protective effects on RA is still unclear. To investigate the effects of moxibustion on RA, moxibustion was applied to Zusanli (ST36) and Shenshu (BL23) acupoints in a RA rabbit model. HE staining of articular cartilage showed that moxibustion alleviated the cartilage degradation and bone destruction. In addition, moxibustion decreased the osteoclast number in RA rabbits. Real-time PCR revealed that moxibustion decreased the expression of RANKL mRNA while increased the expression of OPG mRNA, indicating a restoration of the balance between osteogenesis and osteoclastogenesis. Taken together, our results indicated that moxibustion had promising antiarthritic effects and could be an useful alternative method in RA therapeutics.

scholarly journals Effects of intra‑articular pulsed radiofrequency current administration on a rabbit model of rheumatoid arthritis

2020 ◽  
Vol 20 (5) ◽  
pp. 1-1
Author(s):  
Hee Kyung Cho ◽  
Gi-Young Park ◽  
Woo Jung Sung ◽  
Sang Gyu Kawk ◽  
Won Bin Jung
Keyword(s):  
Rheumatoid Arthritis ◽  
Rabbit Model ◽  
Pulsed Radiofrequency ◽  
Radiofrequency Current

Utilization of longitudinal ultrasound measurements to quantify changes in knee joint synovial vascularity in a rabbit model of rheumatoid arthritis

2020 ◽  
Author(s):  
Hui Liu ◽  
Chao Huang ◽  
Shuqiang Chen ◽  
Qing Zheng ◽  
Yuhong Ye ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Time Course ◽  
Early Stage ◽  
Power Doppler ◽  
Rabbit Model ◽  
Knee Joints ◽  
Control Group ◽  
Vascular Morphology ◽  
Contrast Enhanced ◽  
Positive Correlations

Abstract Objective: This study of rabbits with ovalbumin-induced arthritis (OIA), a model of rheumatoid arthritis (RA), examined the time course of changes in synovial neovascularization based on imaging from power Doppler ultrasound (PDUS) and contrast-enhanced ultrasound (CEUS).Methods: 25 male New Zealand rabbits were in the OIA group and 5 were in the control group. Both rear knee joints of all rabbits were examined using conventional US and CEUS over 16 weeks. The knee synovia of OIA rabbits were sampled by US-guided biopsy, and the expression of CD31 and VEGF were determined by immunohistochemistry. The correlation of microvessel density (CD31 positivity) and VEGF at different times was analyzed using multimodal US.Results: OIA rabbits had increased synovial expression of CD31 and VEGF from week 6 to 12 (P<0.01). During the early stage of CEUS enhancement, dot enhancement was more common on weeks 6 and 8, and strip enhancement was more common on weeks 12 and 16 (P<0.05). There were significant positive correlations of synovial CD31 and VEGF expression with PDI grade, CEUS grade, and peak intensity (PI) (P<0.05 for all).Conclusions: OIA rabbits mimicked early-stage RA at 4 to 8 weeks, middle-stage RA at 8 to 12 weeks, and late-stage RA at 12 to 16 weeks. PDI, CEUS, and PI, especially when combined with CD31 expression, accurately characterized the extent of synovial vascularization. Increased vascular morphology based on CEUS may have value for the early diagnosis of RA.

scholarly journals Possible Origin of Synovial Lining Cell Hyperplasia in Rheumatoid Arthritis

1987 ◽  
Vol 80 (8) ◽  
pp. 477-478
Author(s):  
D W Howat
Keyword(s):  
Rheumatoid Arthritis ◽  
Mitotic Activity ◽  
Tritiated Thymidine ◽  
Rabbit Model ◽  
Synovial Lining ◽  
Cell Hyperplasia ◽  
Synovial Lining Cells ◽  
Synovial Lining Cell

A perplexing feature of rheumatoid arthritis is the increase in the number of synovial lining cells with no mitotic activity. This feature has been investigated in the rabbit model. Rabbits with the established condition were injected into the affected joint with tritiated thymidine and killed either up to 24 hours later, or at 3 or 7 days. The location of labelled cells, detected by autoradiography, showed the label predominantly in the stroma in the former, and mainly in the lining cells in the latter, indicating that the lining cells were derived by recruitment from active cells deep in the stroma.

Liposome-bound APO2L/TRAIL is an effective treatment in a rabbit model of rheumatoid arthritis

2010 ◽  
Vol 62 (8) ◽  
pp. 2272-2282 ◽  
Author(s):  
Luis Martinez-Lostao ◽  
Felí´cito Garcí´a-Alvarez ◽  
Gorka Basáñez ◽  
Elena Alegre-Aguarón ◽  
Paula Desportes ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Effective Treatment ◽  
Rabbit Model

scholarly journals TNF-Alpha in the Locomotor System beyond Joints: High Degree of Involvement in Myositis in a Rabbit Model

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Sture Forsgren ◽  
Lina Renström ◽  
Craig Purdam ◽  
James E. Gaida
Keyword(s):  
Rheumatoid Arthritis ◽  
Animal Model ◽  
Rabbit Model ◽  
Tnf Alpha ◽  
Human Rheumatoid Arthritis ◽  
Locomotor System ◽  
Local Injections ◽  
Inflammatory Infiltrates ◽  
High Degree

The importance of TNF-alpha in arthritis is well documented. It may be that TNF-alpha is also markedly involved in muscle inflammation (myositis). An animal model where this can be investigated is needed. A newly developed rabbit myositis model involving pronounced muscle overuse and local injections of substances having proinflammatory effects was therefore used in the present study. The aim was to investigate the patterns of TNF-alpha expression in the developing myositis and to evaluate the usefulness of this myositis model for further TNF-alpha research. Human rheumatoid arthritis (RA) synovial tissue was examined as a reference. TNF-alpha immunoexpression and TNF-alpha mRNA, visualized via in situ hybridization, were detected in cells in the inflammatory infiltrates of the affected muscle (soleus muscle). Coexistence of TNF-alpha and CD68 immunoreactions was noted, suggesting that the TNF-alpha reactive cells are macrophages. Expression of TNF-alpha mRNA was also noted in muscle fibers and blood vessel walls in areas with inflammation. These findings demonstrate that TNF-alpha is highly involved in the myositis process. The model can be used in further studies evaluating the importance of TNF-alpha in developing myositis.

scholarly journals Value of contrast-enhanced ultrasound for detection of synovial vascularity in experimental rheumatoid arthritis: an exploratory study

2019 ◽  
Vol 47 (11) ◽  
pp. 5740-5751 ◽  
Author(s):  
Hui Liu ◽  
Chao Huang ◽  
Shuqiang Chen ◽  
Qing Zheng ◽  
Yuhong Ye ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Power Doppler ◽  
Rabbit Model ◽  
Knee Joints ◽  
Control Group ◽  
Contrast Enhanced Ultrasound ◽  
Ultrasound Guided ◽  
Synovitis Score ◽  
Contrast Enhanced ◽  
Vessel Count

Objective This study aimed to examine the associations between contrast-enhanced ultrasound (CEUS) imaging and synovial hypervascularity and synovitis score in a rabbit model of antigen-induced arthritis (AIA), compared with power Doppler ultrasound (PDUS). Methods We investigated 50 knee joints in 25 AIA rabbits (AIA group), and 10 knee joints in five sham-injected rabbits (control group). PDUS and CEUS images were evaluated at the 8th week. Ultrasound-guided synovial biopsies were targeted in the area with hypervascularity, and synovial microvessel density (MVD) was evaluated by immunohistochemical staining of CD31. Results The PDUS score was significantly higher in the AIA group (2.61 ± 0.78) compared with the control group (0.50 ± 0.53). CEUS in the AIA group revealed a fast-in/slow-out pattern of contrast enhancement. MVD revealed by CD31+ vessel count and the synovitis score were significantly higher in the AIA group compared with the control group. In the AIA group, CEUS findings showed a better correlation with MVD revealed by CD31+ and synovitis score than PDUS findings. Conclusion CEUS is superior to PDUS for estimating synovial hypervascularity and hyperplasia in experimental rheumatoid arthritis.

Sign in / Sign up

Export Citation Format

Share Document