Effects of intra‑articular pulsed radiofrequency current administration on a rabbit model of rheumatoid arthritis

Rheumatoid arthritis (RA) is a systemic and chronic autoimmune inflammatory disease characterized by severe synovial hyperplasia associated with progressive cartilage degradation. Due to the severe pain and disability caused by RA, effective therapeutic strategies that could simultaneously alleviate the inflammatory response and delay the disease progression are urgently needed. As a major alternative therapy in traditional Chinese medicine, moxibustion has been demonstrated that it could reduce the chronic inflammatory responses of a series of musculoskeletal diseases; however, whether moxibustion has protective effects on RA is still unclear. To investigate the effects of moxibustion on RA, moxibustion was applied to Zusanli (ST36) and Shenshu (BL23) acupoints in a RA rabbit model. HE staining of articular cartilage showed that moxibustion alleviated the cartilage degradation and bone destruction. In addition, moxibustion decreased the osteoclast number in RA rabbits. Real-time PCR revealed that moxibustion decreased the expression of RANKL mRNA while increased the expression of OPG mRNA, indicating a restoration of the balance between osteogenesis and osteoclastogenesis. Taken together, our results indicated that moxibustion had promising antiarthritic effects and could be an useful alternative method in RA therapeutics.

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Pulsed Radiofrequency Current in the Treatment of Pain

Critical Reviews in Physical and Rehabilitation Medicine ◽

10.1615/critrevphysrehabilmed.v23.i1-4.150 ◽

2011 ◽

Vol 23 (1-4) ◽

pp. 213-240 ◽

Cited By ~ 4

Author(s):

Justin Hata ◽

Danielle Perret-Karimi ◽

Cecil DeSilva ◽

Daniel Leung ◽

Naomi Betesh ◽

...

Keyword(s):

Pulsed Radiofrequency ◽

Radiofrequency Current

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Spectroscopic assessment of endogenous porphyrins in a rheumatoid arthritis rabbit model after the application of ALA and ALA-Me

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2012.11.008 ◽

2013 ◽

Vol 119 ◽

pp. 15-21 ◽

Cited By ~ 4

Author(s):

R. Rudys ◽

G. Kirdaitė ◽

S. Bagdonas ◽

L. Leonavičienė ◽

R. Bradūnaitė ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Rabbit Model

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Exposure of the Dorsal Root Ganglion to Pulsed Radiofrequency Current in a Neuropathic Pain Model of Peripheral Nerve Injury

Methods in Molecular Biology - Pain Research ◽

10.1007/978-1-61779-561-9_21 ◽

2012 ◽

pp. 275-284 ◽

Cited By ~ 3

Author(s):

Danielle Perret ◽

Doo-Sik Kim ◽

Kang-Wu Li ◽

Z. David Luo

Keyword(s):

Neuropathic Pain ◽

Dorsal Root Ganglion ◽

Peripheral Nerve ◽

Nerve Injury ◽

Peripheral Nerve Injury ◽

Dorsal Root ◽

Pulsed Radiofrequency ◽

Pain Model ◽

Neuropathic Pain Model ◽

Radiofrequency Current

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Utilization of longitudinal ultrasound measurements to quantify changes in knee joint synovial vascularity in a rabbit model of rheumatoid arthritis

10.21203/rs.3.rs-21820/v1 ◽

2020 ◽

Author(s):

Hui Liu ◽

Chao Huang ◽

Shuqiang Chen ◽

Qing Zheng ◽

Yuhong Ye ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Time Course ◽

Early Stage ◽

Power Doppler ◽

Rabbit Model ◽

Knee Joints ◽

Control Group ◽

Vascular Morphology ◽

Contrast Enhanced ◽

Positive Correlations

Abstract Objective: This study of rabbits with ovalbumin-induced arthritis (OIA), a model of rheumatoid arthritis (RA), examined the time course of changes in synovial neovascularization based on imaging from power Doppler ultrasound (PDUS) and contrast-enhanced ultrasound (CEUS).Methods: 25 male New Zealand rabbits were in the OIA group and 5 were in the control group. Both rear knee joints of all rabbits were examined using conventional US and CEUS over 16 weeks. The knee synovia of OIA rabbits were sampled by US-guided biopsy, and the expression of CD31 and VEGF were determined by immunohistochemistry. The correlation of microvessel density (CD31 positivity) and VEGF at different times was analyzed using multimodal US.Results: OIA rabbits had increased synovial expression of CD31 and VEGF from week 6 to 12 (P<0.01). During the early stage of CEUS enhancement, dot enhancement was more common on weeks 6 and 8, and strip enhancement was more common on weeks 12 and 16 (P<0.05). There were significant positive correlations of synovial CD31 and VEGF expression with PDI grade, CEUS grade, and peak intensity (PI) (P<0.05 for all).Conclusions: OIA rabbits mimicked early-stage RA at 4 to 8 weeks, middle-stage RA at 8 to 12 weeks, and late-stage RA at 12 to 16 weeks. PDI, CEUS, and PI, especially when combined with CD31 expression, accurately characterized the extent of synovial vascularization. Increased vascular morphology based on CEUS may have value for the early diagnosis of RA.

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Monitoring vascular markers of joint inflammation in a rabbit model of rheumatoid arthritis with time-resolved near-infrared spectroscopy

Biomedical Optics 2016 ◽

10.1364/cancer.2016.jtu3a.30 ◽

2016 ◽

Author(s):

Ajay Rajaram ◽

Laura B. Morrison ◽

Keith St Lawrence ◽

Ting-Yim Lee ◽

Mamadou Diop

Keyword(s):

Rheumatoid Arthritis ◽

Infrared Spectroscopy ◽

Near Infrared Spectroscopy ◽

Near Infrared ◽

Rabbit Model ◽

Joint Inflammation ◽

Time Resolved ◽

Vascular Markers

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Pulsed and Continous Radiofrequency Current Adjacent to the Cervical Dorsal Root Ganglion of the Rat Induces Late Cellular Activity in the Dorsal Horn

Anesthesiology ◽

10.1097/00000542-200501000-00021 ◽

2005 ◽

Vol 102 (1) ◽

pp. 125-131 ◽

Cited By ~ 147

Author(s):

Jan Van Zundert ◽

Anton J. A. de Louw ◽

Elbert A. J. Joosten ◽

Alfons G. H. Kessels ◽

Wiel Honig ◽

...

Keyword(s):

Dorsal Root Ganglion ◽

Dorsal Horn ◽

Dorsal Root ◽

Spinal Pain ◽

Pulsed Radiofrequency ◽

Radiofrequency Treatment ◽

Pulsed Radiofrequency Treatment ◽

Significant Difference ◽

Radiofrequency Current ◽

Male Wistar Rats

Background Pulsed radiofrequency treatment has recently been described as a non-neurodestructive or minimally neurodestructive alternative to radiofrequency heat lesions. In clinical practice long-lasting results of pulsed radiofrequency treatment adjacent to the cervical dorsal root ganglion for the management of chronic radicular spinal pain have been reported without neurologic complications. However, the mode of action is unclear. An early (3 h) effect of pulsed radiofrequency as measured by an increase of c-Fos in the pain-processing neurons of the dorsal horn of rats has been described in the literature. This effect was not mediated by tissue heating. The authors investigated a possible late or long-term effect of three different radiofrequency modalities. Methods Cervical laminectomy was performed in 19 male Wistar rats. The cervical dorsal root ganglion was randomly exposed to one of the four interventions: sham, continuous radiofrequency current at 67 centigrades, or pulsed radiofrequency current for 120 s or 8 min. The animals were sacrificed and the spinal cord was prepared for c-Fos labeling 7 days after the intervention. Results The number of c-Fos immunoreactive cells in the dorsal horn was significantly increased in the three different radiofrequency modalities as compared with sham. No significant difference was demonstrated between the three active intervention groups. Conclusions The authors demonstrated a late neuronal activity in the dorsal horn after exposure of the cervical dorsal root ganglion to different radiofrequency modalities, which was not temperature dependent.

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