Photocatalytic antibacterial activity of nano-TiO2 (anatase)-based thin films: Effects on Escherichia coli cells and fatty acids

Traditionally wild plants including Zygophyllum spp., containing many bioactive constituents, are used as medicine. Zygophyllum luntii from Zygophyllaceae family is wildly grown in Oman and has been used for medical purposes. The present study investigated the antibacterial activity of the crude extracts (leaves, stem and roots) and oil (leaves) from Z. luntii. These extracts were tested against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa in well diffusion assays utilizing Muller Hinton Agar (MHA). The hydrocarbon contents and the fatty acid methyl ester (FAME) components of the extracts were also determined by GC-MS. Significant antibacterial activity was observed in the Z. luntii leaves extract and significant differences (F=14.06, df=2, P=0.002) were found among E. coli, P. aeruginosa and S. aureus. The highest inhibition was observed against P. aeruginosa (15.5 ± 2.64 mm) then E. coli (11.25 ± 1.50mm) and S. aureus (3.50±4.72 mm). The Z. luntii extracts showed about 50-60% effectiveness against E. coli and P. aeruginosa compared to Ciprofloxacin. A total of 20, 19 and 8 compounds were detected from leaves, stem and roots extracts, respectively. Heneicosane, Docosane and Tricosane were found in the highest concentration in leaves; HOP-22(29)-EN-3.BETA.-OL and β-Sitosterol in stems, and Docosane and Tricosane in roots of Z. luntii. Nine types of fatty acids methyl esters were detected in oil extracted from leaves and methyl esters of Palmitic acid, Linolenic acid and Oleic acid constitutes 90% of the oil. This is the first report on antibacterial activity and chemical composition of Z. luntii.

Download Full-text

Oxidation of fatty aldehydes to fatty acids by Escherichia coli cells expressing the Vibrio harveyi fatty aldehyde dehydrogenase (FALDH)

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-012-1211-2 ◽

2012 ◽

Vol 29 (3) ◽

pp. 569-575

Author(s):

Markus Buchhaupt ◽

Jan Guder ◽

Fenja Sporleder ◽

Melanie Paetzold ◽

Jens Schrader

Keyword(s):

Escherichia Coli ◽

Fatty Acids ◽

Aldehyde Dehydrogenase ◽

Vibrio Harveyi ◽

Fatty Aldehyde ◽

Escherichia Coli Cells ◽

Fatty Aldehydes

Download Full-text

Corrigendum to “Impact of copper doping in NiO thin films on their structure, morphology, and antibacterial activity against Escherichia Coli.” [Ceram. Int. 46 (2020) 5037-5049]

Ceramics International ◽

10.1016/j.ceramint.2020.11.178 ◽

2020 ◽

Author(s):

Muzamil Aftab ◽

M.Z. Butt ◽

Dilawar Ali ◽

Farooq Bashir ◽

Z.H. Aftab

Keyword(s):

Thin Films ◽

Escherichia Coli ◽

Antibacterial Activity ◽

Copper Doping ◽

Structure Morphology

Download Full-text

Toxicity and Compound Identification of Padinaaustralis Extract

International Journal of Recent Technology and Engineering - 2 ◽

10.35940/ijrte.b1016.0782s719 ◽

2019 ◽

Vol 8 (2S7) ◽

pp. 79-82

Keyword(s):

Escherichia Coli ◽

Fatty Acids ◽

Antibacterial Activity ◽

Active Ingredient ◽

Toxicity Tests ◽

Test Results ◽

Compound Identification ◽

Synthetic Drugs ◽

Identification Test ◽

Test Result

People awareness of the importance of health has increased significantly in the last decade, it forces people to find alternative treatments which are cheaper and safer when compared with the use of synthetic drugs. Research on antibacterial activity of Padina australis against Escherichia coli have been conducted, but it does not identified yet which active compounds in the Padina australis extract that has potential as an antibacterial. Identification phytol compounds from the extracts of Padina australis and toxicity tests have been conducted using BSLT and GCMS methods. The results of this research identify that Padina australis extract with ethanol has antibacterial activity value of LC50 177.83 ppm. Phytochemical test results show that active ingredient of Padina australis extract is terpenoid compounds, and that triterpenoids has potential as an antibacterial. GC-MS test result shows that active compound of Padina australis extract consists of phytol compound which has 90-99% similarity with steroids, phenols, fatty acids, carboxylic acids, hydrocarbons, and proteins. Compound identification test of active Padina australis extracts through GC-MS methods show that phytol compounds are useful as antibacterial and the toxicity test results show that phytol compounds are not cytotoxic.

Download Full-text