Modulating photochemical reactions in Langmuir monolayers of Escherichia coli lipid extract with the binding mechanisms of eosin decyl ester and toluidine blue-O photosensitizers

2021 ◽  
Vol 218 ◽  
pp. 112173
Author(s):  
Lucas G. Moreira ◽  
Alexandre M. Almeida ◽  
Tyler Nield ◽  
Sabrina A. Camacho ◽  
Pedro H.B. Aoki
Keyword(s):  
Escherichia Coli ◽  
Toluidine Blue ◽  
Langmuir Monolayers ◽  
Photochemical Reactions ◽  
Lipid Extract ◽  
Binding Mechanisms

scholarly journals Cytochrome d But Not Cytochrome o Rescues the Toluidine Blue Growth Sensitivity of arc Mutants of Escherichia coli

2009 ◽  
Vol 192 (2) ◽  
pp. 391-399 ◽  
Author(s):  
Adrian F. Alvarez ◽  
Roxana Malpica ◽  
Martha Contreras ◽  
Edgardo Escamilla ◽  
Dimitris Georgellis
Keyword(s):  
Escherichia Coli ◽  
Toluidine Blue ◽  
Response Regulator ◽  
Growth Conditions ◽  
Adaptive Responses ◽  
Cytochrome O ◽  
Insertion Elements ◽  
Blue Growth ◽  
Two Component Signal Transduction ◽  
Cytochrome D

ABSTRACT The Arc (anoxic redox control) two-component signal transduction system, consisting of the ArcB sensor kinase and the ArcA response regulator, allows adaptive responses of Escherichia coli to changes of O2 availability. The arcA gene was previously known as the dye gene because null mutants were growth sensitive to the photosensitizer redox dyes toluidine blue and methylene blue, a phenotype whose molecular basis still remains elusive. In this study we report that the toluidine blue O (TBO) effect on the arc mutants is light independent and observed only during aerobic growth conditions. Moreover, 16 suppressor mutants with restored growth were generated and analyzed. Thirteen of those possessed insertion elements upstream of the cydAB operon, rendering its expression ArcA independent. Also, it was found that, in contrast to cythocrome d, cythocrome o was not able to confer toluidine blue resistance to arc mutants, thereby representing an intriguing difference between the two terminal oxidases. Finally, a mechanism for TBO sensitivity and resistance is discussed.

The prion fragment PrP106-127 adopts a secondary structure typical of aggregated fibrils in langmuir monolayers of brain lipid extract

2020 ◽  
Vol 230 ◽  
pp. 104930
Author(s):  
B. Sandrino ◽  
K. Jochelavicius ◽  
D. Volpati ◽  
S.C. Barbosa ◽  
T.M. Nobre ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Langmuir Monolayers ◽  
Lipid Extract ◽  
Brain Lipid

scholarly journals Cellulose Acetate Containing Toluidine Blue and Rose Bengal Is an Effective Antimicrobial Coating when Exposed to White Light

2006 ◽  
Vol 72 (6) ◽  
pp. 4436-4439 ◽  
Author(s):  
Val�rie Decraene ◽  
Jonathan Pratten ◽  
Michael Wilson
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Clostridium Difficile ◽  
Cellulose Acetate ◽  
Toluidine Blue ◽  
White Light ◽  
Rose Bengal ◽  
Microbial Load ◽  
Hospital Associated Infections

ABSTRACT Simple methods of reducing the microbial load on surfaces in hospitals are needed to reduce the risk of hospital-associated infections. Here we report on the ability of a cellulose acetate coating containing the photosensitizers toluidine blue and rose bengal to kill microbes (Staphylococcus aureus, Escherichia coli, Clostridium difficile, a bacteriophage, and Candida albicans) on its surface when illuminated with white light.

A fast way to track functional OmpF reconstitution in liposomes: Escherichia coli total lipid extract

2015 ◽  
Vol 479 ◽  
pp. 54-59 ◽  
Author(s):  
S.C. Lopes ◽  
M. Ferreira ◽  
C.F. Sousa ◽  
P. Gameiro
Keyword(s):  
Escherichia Coli ◽  
Total Lipid ◽  
Lipid Extract ◽  
Total Lipid Extract
Sign in / Sign up

Export Citation Format

Share Document