Na+ but not Cl− or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings

Exogenous silicon (Si) can enhance plant resistance to various abiotic factors causing osmotic stress. The objective of this research was to evaluate the application of 1 and 2 mM Si to plants under normal conditions and under osmotic stress. Morelos A-98 rice seedlings, were treated with 1 and 2 mM SiO2 for 28 d. Subsequently, half of the plants were subjected to osmotic stress with the addition of 10% polyethylene glycol (PEG) 8000; and continued with the addition of Si (0, 1 and 2 mM SiO2) for both conditions. The application of Si under both conditions increased chlorophyll b in leaves, root volume, as well as fresh and dry biomass of roots. Interestingly, the number of tillers, shoot fresh and dry biomass, shoot water content, concentration of total chlorophyll, chlorophyll a/b ratio, and the concentration of total sugars and proline in shoot increased with the addition of Si under osmotic stress conditions. The addition of Si under normal conditions decreased the concentration of sugars in the roots, K and Mn in roots, and increased the concentration of Fe and Zn in shoots. Therefore, Si can be used as a potent inorganic biostimulant in rice Morelos A-98 since it stimulates plant growth and modulates the concentration of vital biomolecules and essential nutrients.

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Root development and the expression of aquaporin genes in rice seedlings under osmotic stress

Plant Production Science ◽

10.1080/1343943x.2015.1128109 ◽

2016 ◽

Vol 19 (2) ◽

pp. 315-322 ◽

Cited By ~ 3

Author(s):

Maya Matsunami ◽

Kyoko Toyofuku ◽

Junko Ishikawa–Sakurai ◽

Atsushi Ogawa ◽

Toshinori Matsunami ◽

...

Keyword(s):

Osmotic Stress ◽

Root Development ◽

Rice Seedlings

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Light-induced expression of ipt from Agrobacterium tumefaciens results in cytokinin accumulation and osmotic stress symptoms in transgenic tobacco

Plant Molecular Biology ◽

10.1007/bf00042081 ◽

1995 ◽

Vol 28 (5) ◽

pp. 965-965 ◽

Cited By ~ 2

Keyword(s):

Agrobacterium Tumefaciens ◽

Osmotic Stress ◽

Transgenic Tobacco ◽

Induced Expression ◽

Stress Symptoms

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Gluconate enhanced the water uptake and improved the growth of rice seedlings under PEG-induced osmotic stress

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2020.09.037 ◽

2020 ◽

Vol 156 ◽

pp. 514-523

Author(s):

Piwu Li ◽

Bingcui Chen ◽

Feng Ding

Keyword(s):

Osmotic Stress ◽

Water Uptake ◽

Rice Seedlings

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NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

Plant and Soil ◽

10.1007/s11104-008-9874-z ◽

2009 ◽

Vol 320 (1-2) ◽

pp. 103-115 ◽

Cited By ~ 56

Author(s):

Chwan-Yang Hong ◽

Yun-Yang Chao ◽

Min-Yu Yang ◽

Sin-Yuan Cheng ◽

Shih-Chueh Cho ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Oryza Sativa ◽

Abscisic Acid ◽

Glutathione Reductase ◽

Oryza Sativa L ◽

Induced Expression

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Effect of betaine on HSP70 expression and cell survival during adaptation to osmotic stress

Biochemical Journal ◽

10.1042/bj2930553 ◽

1993 ◽

Vol 293 (2) ◽

pp. 553-558 ◽

Cited By ~ 35

Author(s):

P G Petronini ◽

E M De Angelis ◽

A F Borghetti ◽

K P Wheeler

Keyword(s):

Gene Expression ◽

Heat Shock ◽

Osmotic Stress ◽

Cell Survival ◽

Osmotic Pressure ◽

Colony Formation ◽

Hsp70 Expression ◽

Hsp70 Gene ◽

3T3 Cells ◽

Induced Expression

Induced expression of the HSP70 gene in 3T3 and SV-3T3 cells was monitored by measurements of the synthesis of HSP70 and of the cellular contents of both HSP70 and its mRNA. The presence of betaine (N-trimethylglycine) at concentrations of 2.5-25 mM decreased the induction of HSP70 gene expression caused by incubation of 3T3 and SV-3T3 cells in hypertonic (0.5 osM) medium. This effect was accompanied by an enhancement of SV-3T3 cell adaptation, assayed by colony formation, to the hyperosmotic conditions. In contrast, the presence of betaine did not affect HSP70 gene expression induced in these cells by heat shock. After 6 h incubation with 25 mM betaine under hypertonic (0.5 osM) conditions the intracellular concentration of betaine in SV-3T3 cells was about 195 mM, compared with about 70 mM under isotonic (0.3 osM) conditions. Hence, with this concentration of extracellular betaine, the marked increase in the accumulation of betaine within the cells presumably counteracts the imposed osmotic pressure and eliminates the signal that otherwise initiates increased expression of the HSP70 gene.

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Induction of Glutathione Synthesis and Glutathione Reductase Activity by Abiotic Stresses in Maize and Wheat

The Scientific World JOURNAL ◽

10.1100/tsw.2002.812 ◽

2002 ◽

Vol 2 ◽

pp. 1699-1705 ◽

Cited By ~ 20

Author(s):

Gábor Kocsy ◽

Gabriella Szalai ◽

Gabor Gáliba

Keyword(s):

Osmotic Stress ◽

Glutathione Reductase ◽

Temperature Stress ◽

Stress Responses ◽

Abiotic Stresses ◽

Reductase Activity ◽

Cold Treatment ◽

Wheat Variety ◽

High Temperature Stress ◽

Glutathione Reductase Activity

The effect of different abiotic stresses (extreme temperatures and osmotic stress) on the synthesis of glutathione and hydroxymethylglutathione, on the ratio of the reduced to oxidised forms of these thiols (GSH/GSSG, hmGSH/hmGSSG), and on the glutathione reductase (GR) activity was studied in maize and wheat genotypes having different sensitivity to low temperature stress. Cold treatment induced a greater increase in total glutathione (TG) content and in GR activity in tolerant genotypes of both species than in sensitive ones. The GSH/GSSG and hmGSH/hmGSSG ratios were increased by this treatment only in the frost-tolerant wheat variety. High-temperature stress increased the TG content and the GSH/GSSG ratio only in the chilling-sensitive maize genotype, but GR activity was greater after this treatment in both maize genotypes. Osmotic stress resulted in a great increase in the TG content in wheat and the GR activity in maize. The amount of total hydroxymethylglutathione increased following all stress treatments. These results indicate the involvement of these antioxidants in the stress responses of wheat and maize.

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