On the relationship between cell cycle analysis with ergodic principles and age-structured cell population models

2017 ◽  
Vol 414 ◽  
pp. 91-102 ◽  
Author(s):  
K. Kuritz ◽  
D. Stöhr ◽  
N. Pollak ◽  
F. Allgöwer
Keyword(s):  
Cell Cycle ◽  
Cell Population ◽  
Population Models ◽  
Cell Cycle Analysis ◽  
Age Structured ◽  
The Relationship

scholarly journals Sodium azide enhancement of interleukin-2 production

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 815-818 ◽  
Author(s):  
SP Feldman ◽  
R Mertelsmann ◽  
S Venuta ◽  
M Andreeff ◽  
K Welte ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Cell Population ◽  
Sodium Azide ◽  
Human Lymphocytes ◽  
Interleukin 2 ◽  
Final Concentration ◽  
Cell Cycle Analysis ◽  
Normal Human ◽  
Responsive Cell

Abstract The production of large quantities of Interleukin-2 (IL-2) from normal human lymphocytes has been limited by the short production and release period, as well as its absorption by the responsive cell population. We report the utilization of sodium azide (NaN3, 0.01% final concentration) to allow continued production of IL-2 as long as 72–96 hr and thereby increase the yield significantly. Cell cycle analysis performed by flow cytometry indicates that NaN3 blocks cells at the G0- G1 transition and the G1-S transition, depending on the time of addition of NaN3 to the cultures.

scholarly journals Kinetic Cell-Cycle Analysis of a Cultured Mammalian Cell Population

1974 ◽  
Vol 14 (8) ◽  
pp. 607-624 ◽  
Author(s):  
B.V. Bronk ◽  
G.J. Dienes ◽  
R. Schindler ◽  
J.R. Gautschi
Keyword(s):  
Cell Cycle ◽  
Mammalian Cell ◽  
Cell Population ◽  
Cell Cycle Analysis

From cell population models to tumor control probability: Including cell cycle effects

2010 ◽  
Vol 49 (8) ◽  
pp. 1315-1323 ◽  
Author(s):  
Thomas Hillen ◽  
Gerda de Vries ◽  
Jiafen Gong ◽  
Chris Finlay
Keyword(s):  
Cell Cycle ◽  
Cell Population ◽  
Population Models ◽  
Tumor Control ◽  
Tumor Control Probability

scholarly journals Cell Cycle Analysis of Epstein-Barr Virus-Infected Cells following Treatment with Lytic Cycle-Inducing Agents

2001 ◽  
Vol 75 (10) ◽  
pp. 4482-4489 ◽  
Author(s):  
Antonio Rodriguez ◽  
Eun Joo Jung ◽  
Erik K. Flemington
Keyword(s):  
Cell Cycle ◽  
Immediate Early Gene ◽  
Lytic Cycle ◽  
Early Gene ◽  
Immediate Early ◽  
Cell Cycle Analysis ◽  
Cycle Progression ◽  
Barr Virus ◽  
Epstein Barr ◽  
The Relationship

ABSTRACT While Epstein-Barr virus (EBV) latency-associated gene expression is associated with cell cycle progression, the relationship between the EBV lytic program and the cell cycle is less clear. Using four different EBV lytic induction systems, we address the relationship between lytic cycle activation and the cell cycle. In three of these systems, G0 or G1 cell growth arrest signaling is observed prior to detection of the EBV immediate-early gene product Zta. In tetradecanoyl phorbol acetate-treated P3HR1 cultures and in 5-iodo-2′-deoxyuridine-treated NPC-KT cultures, cell cycle analysis of Zta-expressing cell populations showed a significant G1bias during the early stages of lytic cycle progression. In contrast, treatment of the cell line Akata with anti-immunoglobulin (Ig) results in rapid induction of immediate-early gene expression, and accordingly, activation of the immediate-early gene product Zta precedes significant anti-Ig-induced cell cycle effects. Nevertheless, cell cycle analysis of the Zta-expressing population following anti-Ig treatment shows a bias for cells in G1, indicating that anti-Ig-mediated induction of Zta occurs more efficiently in cells traversing G1. Last, although 5-azacytidine treatment of Rael cells results in a G1 arrest in the total cell population which precedes the induction of Zta, cell cycle analysis of the Zta-expressing population shows a significant bias for cells with an apparent G2/M DNA content. This bias may result, in part, from activation of Zta expression following demethylation of the Zta promoter during S-phase. Together, these studies indicate that induction of Zta occurs through several distinct mechanisms, some of which may involve checkpoint signaling.

scholarly journals Sodium azide enhancement of interleukin-2 production

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 815-818
Author(s):  
SP Feldman ◽  
R Mertelsmann ◽  
S Venuta ◽  
M Andreeff ◽  
K Welte ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Cell Population ◽  
Sodium Azide ◽  
Human Lymphocytes ◽  
Interleukin 2 ◽  
Final Concentration ◽  
Cell Cycle Analysis ◽  
Normal Human ◽  
Responsive Cell

The production of large quantities of Interleukin-2 (IL-2) from normal human lymphocytes has been limited by the short production and release period, as well as its absorption by the responsive cell population. We report the utilization of sodium azide (NaN3, 0.01% final concentration) to allow continued production of IL-2 as long as 72–96 hr and thereby increase the yield significantly. Cell cycle analysis performed by flow cytometry indicates that NaN3 blocks cells at the G0- G1 transition and the G1-S transition, depending on the time of addition of NaN3 to the cultures.

scholarly journals A Survey of Cell Population Dynamics

1997 ◽  
Vol 1 (1) ◽  
pp. 35-51 ◽  
Author(s):  
O. Arino ◽  
E. Sánchez
Keyword(s):  
Cell Cycle ◽  
Population Dynamics ◽  
Cell Population ◽  
Linear Models ◽  
Population Models ◽  
Cell Dynamics ◽  
Cell Population Dynamics ◽  
Non Linear

A Survey of cell-population models is presented. The so-called cell cycle is discussed, including some models which are not population models but which contribute to the better understanding proliferation of cell dynamics. Classical linear models of structured cell-population models are described with a review of classical results and a comparison between different approaches. Finally, some non-linear models are also developed.

Identification and Expression Analysis of CD73 Inhibitors in Cervical Cancer

2020 ◽  
Vol 16 ◽  
Author(s):  
Jamshed Iqbal ◽  
Ayesha Basharat ◽  
Sehrish Bano ◽  
Syed Mobasher Ali Abid ◽  
Julie Pelletier ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Western Blot ◽  
Hela Cell ◽  
Cell Line ◽  
Cell Apoptosis ◽  
Immunofluorescence Staining ◽  
Cell Cycle Analysis ◽  
Hela Cell Line ◽  
Cell Line Hela

Aims: The present study was conducted to examine the inhibitory effects of synthesized sulfonylhydrazones on the expression of CD73 (ecto-5′-NT). Background: CD73 (ecto-5′-NT) represents the most significant class of ecto-nucleotidases which are mainly responsible for dephosphorylation of adenosine monophosphate to adenosine. Inhibition of CD73 played an important role in the treatment of cancer, autoimmune disorders, precancerous syndromes, and some other diseases associated with CD73 activity. Objective: Keeping in view the significance of CD73 inhibitor in the treatment of cervical cancer, a series of sulfonylhydrazones (3a-3i) derivatives synthesized from 3-formylchromones were evaluated. Methods: All sulfonylhydrazones (3a-3i) were evaluated for their inhibitory activity towards CD73 (ecto-5′-NT) by the malachite green assay and their cytotoxic effect was investigated on HeLa cell line using MTT assay. Secondly, most potent compound was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis. After that, CD73 mRNA and protein expression were analyzed by real-time PCR and Western blot. Results: Among all compounds, 3h, 3e, 3b, and 3c were found the most active against rat-ecto-5′-NT (CD73) enzyme with IC50 (µM) values of 0.70 ± 0.06 µM, 0.87 ± 0.05 µM, 0.39 ± 0.02 µM and 0.33 ± 0.03 µM, respectively. These derivatives were further evaluated for their cytotoxic potential against cancer cell line (HeLa). Compound 3h and 3c showed the cytotoxicity at IC50 value of 30.20 ± 3.11 µM and 86.02 ± 7.11 µM, respectively. Furthermore, compound 3h was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis which showed promising apoptotic effect in HeLa cells. Additionally, compound 3h was further investigated for its effect on expression of CD73 using qRT-PCR and western blot. Conclusion: Among all synthesized compounds (3a-3i), Compound 3h (E)-N'-((6-ethyl-4-oxo-4H-chromen-3-yl) methylene)-4-methylbenzenesulfonohydrazide was identified as most potent compound. Additional expression studies conducted on HeLa cell line proved that this compound successfully decreased the expression level of CD73 and thus inhibiting the growth and proliferation of cancer cells.

Novel Anthraquinone Derivatives as Dual Inhibitors of Topoisomerase 2 and Casein Kinase 2: In Silico Studies, Synthesis and Biological Evaluation on Leukemic Cell Lines

2019 ◽  
Vol 18 (11) ◽  
pp. 1551-1562 ◽  
Author(s):  
Abbas Kabir ◽  
Kalpana Tilekar ◽  
Neha Upadhyay ◽  
C.S. Ramaa
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Cell Lines ◽  
Biological Evaluation ◽  
Casein Kinase ◽  
Casein Kinase 2 ◽  
Cell Cycle Analysis ◽  
Protein Targets ◽  
Topoisomerase 2 ◽  
Dual Inhibitors

Background: Cancer being a complex disease, single targeting agents remain unsuccessful. This calls for “multiple targeting”, wherein a single drug is so designed that it will modulate the activity of multiple protein targets. Topoisomerase 2 (Top2) helps in removing DNA tangles and super-coiling during cellular replication, Casein Kinase 2 (CK2) is involved in the phosphorylation of a multitude of protein targets. Thus, in the present work, we have tried to develop dual inhibitors of Top2 and CK2. Objective: With this view, in the present work, 2 human proteins, Top2 and CK2 have been targeted to achieve the anti-proliferative effects. Methods: Novel 1-acetylamidoanthraquinone (3a-3y) derivatives were designed, synthesized and their structures were elucidated by analytical and spectral characterization techniques (FTIR, 1H NMR, 13C NMR and Mass Spectroscopy). The synthesized compounds were then subjected to evaluation of cytotoxic potential by the Sulforhodamine B (SRB) protein assay, using HL60 and K562 cell lines. Ten compounds were analyzed for Top2, CK2 enzyme inhibitory potential. Further, top three compounds were subjected to cell cycle analysis. Results: The compounds 3a to 3c, 3e, 3f, 3i to 3p, 3t and 3x showed excellent cytotoxic activity to HL-60 cell line indicating their high anti-proliferative potential in AML. The compounds 3a to 3c, 3e, 3f, 3i to 3p and 3y have shown good to moderate activity on K-562 cell line. Compounds 3e, 3f, 3i, 3x and 3y were found more cytotoxic than standard doxorubicin. In cell cycle analysis, the cells (79-85%) were found to arrest in the G0/G1 phase. Conclusion: We have successfully designed, synthesized, purified and structurally characterized 1- acetylamidoanthraquinone derivatives. Even though our compounds need design optimization to further increase enzyme inhibition, their overall anti-proliferative effects were found to be encouraging.

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