Biodistribution and toxickinetic variances of chemical and green Copper oxide nanoparticles in vitro and in vivo

2020 ◽

Vol 20 ◽

Author(s):

Seyedeh R. Alizadeh ◽

Mohammad A. Ebrahimzadeh

Keyword(s):

Drug Delivery ◽

Cancer Therapy ◽

Copper Oxide ◽

Medical Science ◽

Copper Oxide Nanoparticles ◽

Cuo Nanoparticles ◽

Oxide Nanoparticles ◽

Anticancer Activities

Background: Cancer is defined as an abnormal/uncontrolled cell growth that shows rapid cell division. This disease is annually recognized in more than ten million people. Nanomaterials can be used as new strategies for cancer therapy. Nanostructured devices have developed for drug delivery and controlled release and created novel anticancer chemotherapies. Nanomaterials were taken into consideration because of their new properties, containing a large specific surface area and high reactivity. Copper oxide nanoparticles (CuONPs) have potential applications in many fields like heterogeneous catalysis, antibacterial, anticancer, antioxidant, antifungal, antiviral, imaging agents, and drug delivery agents in biomedicine. CuONPs display different physical properties, such as hightemperature superconductivity, electron correlation effects, and spin dynamics. NPs can be synthesized using different methods like physical, chemical, and biological methods. Methods: Copper oxide nanoparticles (CuONPs) have been suggested for its broad usage in biomedical applications. In this review, we tried to exhibit the results of significant anticancer activity of green synthesized CuONPs and their characterization by different analytical techniques such as UV-Vis, FT-IR, XRD, EDAX, DLS, SEM, and TEM. Results: The green method for the synthesis of CuO nanoparticles as eco-friendly, cost-effective, and facile method is the more effective method. Synthesized CuONPs from this method have an appropriate size and shape. The Green synthesized CuONPs exhibited high potential against several breast cancer (AMJ-13, MCF-7, and HBL-100 cell lines), cervical cancer (HeLa), colon cancer (HCT-116), gastric cancer (human adenocarcinoma AGS cell line), lung cancer (A549), leukemia cancer, and other cancers with the main toxicity approach of increasing ROS production. Conclusion: The present review confirms the importance of green synthesized CuO nanoparticles in medical science especially cancer therapy that exhibited high activity against different cancer in both in vitro and in vivo. The main toxicity approach of CuONPs is increasing the production of reactive oxygen species (ROS). It needs to perform more studies about in vivo cancer therapy and following clinical trial testing in the future. We believe that green synthesized CuO nanoparticles can be used for the improvement of different diseases.

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Folic acid modified copper oxide nanoparticles for targeted delivery in in vitro and in vivo systems

RSC Advances ◽

10.1039/c5ra08110f ◽

2015 ◽

Vol 5 (83) ◽

pp. 68169-68178 ◽

Cited By ~ 26

Author(s):

Dipranjan Laha ◽

Arindam Pramanik ◽

Sourav Chattopadhyay ◽

Sandip kumar Dash ◽

Somenath Roy ◽

...

Keyword(s):

Breast Cancer ◽

Folic Acid ◽

Cancer Therapy ◽

Copper Oxide ◽

Targeted Delivery ◽

Copper Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Breast Cancer Therapy

Targeted delivery of copper oxide nanoparticles for breast cancer therapy.

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Copper Oxide Nanoparticles Stimulate Cytotoxicity and Apoptosis in Glial Cancer Cell Line

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v17i1.37126 ◽

2018 ◽

Vol 17 (1) ◽

pp. 105-111 ◽

Cited By ~ 5

Author(s):

Nasim Rahmani Kukia ◽

Ardeshir Abbasi ◽

Seyyed Maysam Abtahi Froushani

Keyword(s):

Copper Oxide ◽

Cancer Cells ◽

Cell Line ◽

Cytotoxic Effect ◽

Cancer Cell Line ◽

Copper Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Cuo Nps

Due to cytotoxic potential, Copper Oxide Nanoparticles (CuO NPs) have recently been studied in various in vivo and in culture cell line. Also, CuO has received much attention in cancer therapy. We aimed to evaluate the cytotoxicity of CuO NPs on glial cancer (B92) cell line. B92 cancer cells were cultured with CuO NPs at different concentrations (5, 10, and 20 μg/ml) with 30 and 60 nm particle size. Then, cancer cells were incubated for 24 hrs. The apoptosis and cytotoxicity of cells were estimated by acridine orange/propidium iodide staining and MTT assay, respectively. Both sizes of CuO NPs had cytotoxic effect. Even with the lowest concentration, the cytotoxic impact accommodated 32% of cell apoptosis with 30 nm size. When the concentration of CuO NPs increased, viability decreased and apoptosis increased. However, these amounts have no significant changes in the concentration of 10 to 20 μg/ml between two particle sizes (30 and 60 nm). The IC50 was decreased as the size of particles increased, but there was no significant change. This finding suggests that exposure to CuO NPs had significant cytotoxic effect with the sizes tested when compared to unexposed control in a way that the smaller size and higher concentration exerted the maximum cytotoxic effects. It seems that augmentation may not have any impact on their in vitro cytotoxicity.Dhaka Univ. J. Pharm. Sci. 17(1): 105-111, 2018 (June)

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Statistical Optimization of Copper Oxide Nanoparticles Using Response Surface Methodology and Box–Behnken Design Towards In Vitro and In Vivo Toxicity Assessment

Biointerface Research in Applied Chemistry ◽

10.33263/briac113.1002710039 ◽

2020 ◽

Vol 11 (3) ◽

pp. 10027-10039

Keyword(s):

Response Surface Methodology ◽

Copper Oxide ◽

Response Surface ◽

Copper Oxide Nanoparticles ◽

Toxicity Assessment ◽

P Value ◽

Oxide Nanoparticles ◽

Box Behnken Design

The current study focusses on the optimization of Copper oxide nanoparticles (CuO NPs) biosynthesis with Alternanthera sessilis (L.) extract using response surface methodology (RSM). The effect of time, pH, and extract to metal concentration ratio on the yield of synthesized nanoparticles (NPs) were estimated using Box–Behnken design. The influence of each of the parameters, as mentioned earlier, was determined by synthesizing nanoparticles under different conditions. A total of 29 experimental runs were carried out to estimate the crucial parameters. Extract to the metal ratio was found to be the vital parameter for yield optimization based on the p-values (p-value < 0.05). The physicochemical property of NPs, like size, was estimated to be in the range of 10-20 nm. In zebrafish, 48 hpf and 72 hpf were measured at 90 µM to reduce dysfunction and mortality during organ development. These results can have a valuable impact on eco-toxicological effects.

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Autophagy deficiency exacerbates acute lung injury induced by copper oxide nanoparticles

Journal of Nanobiotechnology ◽

10.1186/s12951-021-00909-1 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Junting Xiao ◽

Baijie Tu ◽

Xin Zhou ◽

Xuejun Jiang ◽

Ge Xu ◽

...

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Copper Oxide ◽

Copper Ions ◽

Intratracheal Instillation ◽

Copper Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Dependent Manner

AbstractCopper oxide nanoparticles (CuONPs) are one of the widely used metal nanoparticles in the industrial and commercial fields. Autophagy is an intracellular degradation system that delivers cytoplasmic constituents to the lysosome and has been linked to nanoparticles-induced toxicity. In particular, the roles of autophagy in response to CuONPs have been explored in vitro, although the conclusions are controversial. To clarify the role of autophagy in CuONPs-induced acute lung injury, microtubule-associated protein 1 light chain 3 beta (Map1lc3b or lc3b) knockout mice and their corresponding wild type mice are applied. Our results showed that single-dose intratracheal instillation of CuONPs with dosages of 1.25, 2.5 or 5 mg/kg caused acute lung injury 3 days after treatment in a dose-dependent manner, as evidenced by deteriorative lung histopathology, more infiltration of macrophage cells, increased oxidative stress and copper ions. Loss of lc3b resulted in aggravated lung injury induced by CuONPs, which was probably due to the blockade of mitophagy and consequently the accumulation of aberrant mitochondria with overloaded copper ions. Our study provides the first in vivo evidence that autophagy deficiency exacerbates CuONPs-induced acute lung injury, and highlights that targeting autophagy is a meaningful strategy against CuONPs-associated respiratory toxicity.

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