Optimization of trypsins for influenza A/H1N1 virus replication in MDCK SI-6 cells, a novel MDCK cell line

2018 ◽  
Vol 252 ◽  
pp. 94-99
Author(s):  
Viska I. Iskandar ◽  
Yutaka Sasaki ◽  
Naoto Yoshino ◽  
Raden Z.R. Abubakar ◽  
Shigehiro Sato ◽  
...  
Keyword(s):  
Cell Line ◽  
Virus Replication ◽  
Mdck Cell ◽  
Influenza A ◽  
H1n1 Virus ◽  
Mdck Cell Line ◽  
Influenza A H1n1

scholarly journals IN VITRO ANTI-VIRAL ACTIVITY OF HEXETIDINE (BACTIDOL®) ORAL MOUTHWASH AGAINST HUMAN CORONAVIRUS OC43 AND INFLUENZA A (H1N1) VIRUS

2021 ◽  
Author(s):  
Marohren C Tobias -Altura ◽  
Corazon A Ngelangel
Keyword(s):  
Mdck Cell ◽  
Influenza A ◽  
H1n1 Virus ◽  
Clinical Strain ◽  
Human Coronavirus ◽  
Viral Activity ◽  
Study Results ◽  
Influenza A H1n1 ◽  
Mucous Membranes

ABSTRACTMouthwashes are used to decrease oral cavity microbial load due to their antiseptic properties. Hexetidine is a broad-spectrum antiseptic used for minor infections of mucous membranes, and in particular as a 0.1% mouthwash for local infections and oral hygiene.This study determined the anti-viral activity of the mouthwash hexetidine (Bactidol®), specifically in reducing viral concentration of Human Coronavirus OC43 (HCoV OC43; ATCC® VR-1558™) and Influenza A virus (IAV H1N1; clinical strain) in Vero 6 and MDCK cell cultures respectively, using in-vitro suspension assay (ASTM E-1052-11) designed to evaluate virucidal property of microbicides like hexetidine.Study results indicated that hexetidine was able to reduce infectivity of HCoV OC43 and IAV H1N1 at 25%, 50% and 100% concentrations by more than 80% at 15- and 30-seconds exposure times. One hundred percent (100%) concentration of hexetidine was found to be cytotoxic to MDCK cell line used for IAV H1N1 propagation. Hexetidine-treated cell lines achieved >80% survival rate for MDCK and Vero E6 at a contact time of 15 seconds and 30 seconds (which are the approximate times of gargling with hexetidine mouthwash).The anti-viral activity of hexetidine mouthwash against other more virulent or pathogenic coronaviruses like SARS-CoV-2 can be explored further.

scholarly journals Proteasomes raise the microtubule dynamics in influenza A (H1N1) virus-infected LLC-MK2 cells

2015 ◽  
Vol 20 (5) ◽  
Author(s):  
Flora De Conto ◽  
Carlo Chezzi ◽  
Alessandra Fazzi ◽  
Sergey V. Razin ◽  
Maria Cristina Arcangeletti ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Virus Replication ◽  
Influenza A ◽  
H1n1 Virus ◽  
A549 Cells ◽  
Proteasome Activity ◽  
Cellular Model ◽  
Cell Infection ◽  
Cellular Models ◽  
Influenza A H1n1

AbstractThe dynamics of microtubule networks are known to have an impact on replication of influenza A virus in some cellular models. Here we present evidence suggesting that at late stages of LLC-MK2 cell infection by influenza A (H1N1) virus the ubiquitin-proteasome protein degradation system participates in destabilization of microtubules, and favours virus replication. Chemical inhibition of proteasome activity partially suppresses influenza A virus replication, while stimulation of proteasome activity favours influenza A virus replication. Conversely, in another cellular model, A549 cells, inhibitors and activators of proteasomes have a small effect on influenza A virus replication. These data suggest that influenza A virus might take selective advantage of proteasome functions in order to set up a favourable cytoskeletal “environment” for its replication and spread. Furthermore, the relationship between influenza virus and the host cell is likely to depend on both the cellular model and the virus strain.

In-vitro validation of self designed siRNA targeting non-structural 1 gene of Influenza A virus

2015 ◽  
Vol 4 (6) ◽  
pp. 315-322
Author(s):  
Bhawana Jain ◽  
Amita Jain ◽  
Om Prakash ◽  
Ajay K. Singh ◽  
Tanushree Dangi ◽  
...  
Keyword(s):  
Cell Line ◽  
Influenza A Virus ◽  
Mdck Cell ◽  
Influenza A ◽  
Screening Method ◽  
Viral Gene ◽  
Mdck Cell Line ◽  
Ns1 Gene ◽  
Conserved Region

  The genomic variability makes Influenza A virus (IAV) difficult to be con-trolled by existing vaccines or anti-influenza drugs. Viral gene targeting siRNA induces the RNAi mechanism in the host and silents the gene by cleaving mRNA. Objective was to develop siRNA targeting non-structural 1 gene and to validate its efficiency in vitro. siRNA was designed rationally, targeting the most conserved region (delineated with the help of multiple sequence align-ment) of NS1 gene of IAV strains. To choose the most efficient siRNA, three levels screening method was developed. Ultimately one siRNA duplex was selected on the basis of its unique position in conserved region. siRNA effica-cy was confirmed in vitro on commonly used Madin Darby Canine Kidney (MDCK) cell line for IAV propagation using two clinical isolates i.e. Influenza A/H3N2 [A/India/LKO864/2011(H3N2)] and Influenza A/pdmH1N1 [A/India/LKO2151/2012(H1N1)]. Of total 173 strains worldwide and 30 strains from India, 32 bp long (position 561 - 592) conserved region was identified. The longest ORF of NS1 gene was targeted by the selected siRNA, which showed 65.5% inhibition in replication of Influenza A/pdmH1N1 and 67.2% inhibition in replication of Influenza A/H3N2 at 48 hpi on MDCK cell line. This study shows that siRNA targeting NS1 may be quite effective in controlling IAV rep-lication so can be used as anti-IAV therapeutic agent.

scholarly journals Progression and Trends in Virus from Influenza A to COVID-19: An Overview of Recent Studies

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1145
Author(s):  
Hakimeh Baghaei Daemi ◽  
Muhammad Fakhar-e-Alam Kulyar ◽  
Xinlin He ◽  
Chengfei Li ◽  
Morteza Karimpour ◽  
...  
Keyword(s):  
Influenza A ◽  
H1n1 Virus ◽  
World Health ◽  
H1n1 Pandemic ◽  
Global Pandemic ◽  
First Case ◽  
Influenza A H1n1 ◽  
The World ◽  
Novel Coronavirus ◽  
Health Organization

Influenza is a highly known contagious viral infection that has been responsible for the death of many people in history with pandemics. These pandemics have been occurring every 10 to 30 years in the last century. The most recent global pandemic prior to COVID-19 was the 2009 influenza A (H1N1) pandemic. A decade ago, the H1N1 virus caused 12,500 deaths in just 19 months globally. Now, again, the world has been challenged with another pandemic. Since December 2019, the first case of a novel coronavirus (COVID-19) infection was detected in Wuhan. This infection has risen rapidly throughout the world; even the World Health Organization (WHO) announced COVID-19 as a worldwide emergency to ensure human health and public safety. This review article aims to discuss important issues relating to COVID-19, including clinical, epidemiological, and pathological features of COVID-19 and recent progress in diagnosis and treatment approaches for the COVID-19 infection. We also highlight key similarities and differences between COVID-19 and influenza A to ensure the theoretical and practical details of COVID-19.

scholarly journals Influenza A (H1N1) virus infection and TNF-308, IL6, and IL8 polymorphisms in Egyptian population: a case–control study

2019 ◽  
Vol 80 (1) ◽  
Author(s):  
Shaimaa Moustafa Elsayed ◽  
Omayma Mohamed Hassanein ◽  
Nagwa Hassan Ali Hassan
Keyword(s):  
Influenza A ◽  
Case Control Study ◽  
H1n1 Virus ◽  
Case Control ◽  
Severe Illness ◽  
Study Results ◽  
Influenza A H1n1 ◽  
Pandemic Strains ◽  
Study Participants ◽  
Control Study

Abstract Background The importance of influenza is increasing mainly because of the appearance of novel pandemic strains such as swine and avian. Each year, influenza has spread around the world causing about 250,000–500,000 deaths and more than 5 million cases of severe illness. The objective is as follows: evaluating the outcomes of patients with influenza A (H1N1) virus in relation to certain TNF-308, IL6, and IL8 polymorphisms and identifying the associated factors with the severe outcome. Subject and methods This is a case–control study. The cases were patients confirmed by real-time polymerase chain reaction (RT-PCR) to be influenza A (H1N1) virus infected. The controls were healthy individuals. Medical history and outcome of the disease was registered. In all study participants, polymorphisms of TNF rs1800629, IL6 rs18138879, and IL8 rs4073; odds ratio (OR); and the 95% confidence interval (95% CI) were calculated. Results Infection with influenza A (H1N1) virus was associated more with the following genotypes: TNF-308 AA (OR = 4.041; 95% CI = 1.215–13.4) and IL8 AA (OR = 3.273; 95% CI = 1.372–7.805). According to our study results, HCV (OR = 3.2, 95% CI 1.2–8.5), renal disease (OR = 3.4, 95% CI 0.9–13.6), cancer (OR = 3.1, 95% CI 0.3–31.1), TB (OR = 8.4, 95% CI 1.8–39.7), ICU (OR = 2.9, 95%1.2–7.1), and mortality (OR = 7.9, 95% CI 0.9–67.4) are considered as risk factors for influenza A (H1N1)-infected patients. Conclusions Our findings concluded that TNF-308 (AA) and IL8 (AA) polymorphisms may increase the susceptibility to be infected with H1N1influenza virus.

scholarly journals Ultrastructural and Histopathological Studies of 2009 Pandemic Influenza A (H1N1) Virus

2010 ◽  
Vol 16 (S2) ◽  
pp. 1122-1123
Author(s):  
CS Goldsmith ◽  
MG Metcalfe ◽  
W-J Shieh ◽  
DM Blau ◽  
DC Rollin ◽  
...  
Keyword(s):  
Pandemic Influenza ◽  
Influenza A ◽  
H1n1 Virus ◽  
Influenza A H1n1 ◽  
Histopathological Studies

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

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