Neuroprotective effect of epalrestat mediated through oxidative stress markers, cytokines and TAU protein levels in diabetic rats

Objectives: The aim of this study was to evaluate the effects of resistance, endurance, and concurrent exercise on oxidative stress markers and histological changes of the intestine after morphine withdrawal in rats. Methods: A total of 30 male Wistar rats were randomly divided into 5 groups (n=6) including healthy control, withdrawal (rat received morphine for 21 days and 8 weeks of withdrawal period), withdrawal + endurance exercises, withdrawal + resistance exercises, and withdrawal + concurrent exercises. The rats practiced endurance, resistance, and concurrent exercises for 10 weeks. Then, their intestines were removed and used for biochemical and histological analysis. Next, several factors were measured such as total protein levels, malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAC), and total oxidant status (TOS). Finally, the morphological alteration of intestine was examined under the light microscope. Results: Morphine withdrawal significantly increased the levels of MDA in the intestine of withdrawal rats compared to those of the control group while endurance, resistance, and concurrent exercise reduced the MDA levels in the intestine. In addition, morphine withdrawal led to a decrease in TAC and GSH levels in the intestine compared to control rats whereas endurance, resistance, and concurrent exercise noticeably increased TAC and GSH levels. Interestingly, the change in the concurrent group was more significant. Moreover, the levels of TOS demonstrated a significant increase in the addicted rat as compared to the control group. Conversely, endurance, resistance, and concurrent exercise significantly decreased TOS levels and the reduction was more significant in the concurrent group. Finally, the intestine of withdrawal rat was morphologically abnormal while it restored by the exercise. Conclusion: Overall, endurance, resistance, and concurrent exercise significantly normalized oxidative stress and the morphological changes of the intestine in withdrawal rats.

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Neuroprotective effect of methanolic extract of Sargassum wightii on rotenone-induced parkinsonism-like symptoms in Wistar albino rats

Journal of Herbmed Pharmacology ◽

10.34172/jhp.2021.54 ◽

2021 ◽

Vol 10 (4) ◽

pp. 468-475

Author(s):

Sradhasini Rout ◽

Bandana Rath ◽

Subrat Kumar Bhattamisra ◽

Anjan Kumar ◽

Ishani Rath ◽

...

Keyword(s):

Oxidative Stress ◽

Motor Coordination ◽

Methanolic Extract ◽

Neuroprotective Effect ◽

Albino Rats ◽

Dopamine Level ◽

Oxidative Stress Markers ◽

Sargassum Wightii ◽

Stress Markers ◽

Wistar Albino Rats

Introduction: The pathogenesis of Parkinson’s disease (PD) is multifactorial in which oxidative stress, neuroinflammation, and mitochondrial dysfunction are the leading factors. Currently, the antioxidant and anti-inflammatory agents of natural sources as neuroprotectants have raised much attention. The current study aimed to explore the neuroprotective effect of methanolic extract of Sargassum wightii in male Wistar albino rats against rotenone-induced PD. Methods: The rats were administered with rotenone (10 mg/kg orally) daily for 28 days to induce PD. S. wightii (200 mg/kg and 400 mg/kg) and levodopa+carbidopa combination (10 mg/kg) were administered to different groups of rats one hour prior to rotenone for 28 days. Behavioral parameters (akinesia, tremor, motor coordination, and locomotor activities) and body weight were recorded on days 14th and 28th of drug treatment. On the 28th day, the animals were sacrificed for the neurobiochemical analyses of brain tissue. Results: Rotenone treatment caused a significant reduction in behavioural parameters (P < 0.001), neurochemical deficits (P < 0.001), and elevation of oxidative stress markers (P < 0.001) in the brain. Pre-treatment with S. wightii at 200 mg/kg and 400 mg/kg doses significantly attenuated the rotenone-induced behavioral alterations and restored the mitochondrial NADH dehydrogenase activity and dopamine level in the striatum (P < 0.001). Moreover, 400 mg/kg of S. wightii restored the rotenone-induced increased oxidative stress markers like malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH) in the striatum (P < 0.01). Conclusion: S. wightii has provided a neuroprotective effect, probably by virtue of its antioxidant and dopamine restoring potential. Hence, it may offer a promising and new therapeutic lead for the treatment of PD but needs further research.

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“2-(4-Fluorobenzamido)-4-methylthiazole-5-carboxylic acid” a novel thiazole compound, ameliorates insulin sensitivity and hyperlipidaemia in streptozotocin-induced diabetic rats: Plausible role of inflammatory and oxidative stress markers

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2017.09.014 ◽

2017 ◽

Vol 95 ◽

pp. 1232-1241 ◽

Cited By ~ 1

Author(s):

Yam Nath Paudel ◽

Md. Rahmat Ali ◽

Mohammad Adil ◽

Sandhya Bawa ◽

Manju Sharma

Keyword(s):

Oxidative Stress ◽

Insulin Sensitivity ◽

Carboxylic Acid ◽

Diabetic Rats ◽

Oxidative Stress Markers ◽

Stress Markers ◽

And Oxidative Stress

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ANTIHYPERGLYCEMIC AND ANTIOXIDATIVE EFFECTS OF LYGODIUM MICROPHYLLUM IN ALLOXAN INDUCED DIABETIC RATS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i12.29232 ◽

2018 ◽

Vol 10 (12) ◽

pp. 75

Author(s):

D. G. Syahidah Nadiah Binti Abdull Majid ◽

Mohammad Iqbal

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Blood Glucose ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Control Group ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Antioxidative Effects ◽

And Oxidative Stress

Objective: The antihyperglycemic and antioxidative effects of L. microphyllum were evaluated by using in vivo methods in normal and alloxan induced diabetic rats.Methods: Diabetes was induced in Sprague Dawley rats by injecting alloxan through intravenous (i. v) at a dose of 100 mg/kg of body weight. Aqueous extract of L. microphyllum at different doses (400, 200 and 100 mg/kg of body weight) was administered orally (orogastric intubation) for 14 d. Blood glucose and oxidative stress markers were measured. Hematoxylin and eosin staining method were used to examine the pancreatic tissues.Results: At the 14 d interval, fasting blood glucose showed a reduction in serum glucose levels in animals pretreated with L. microphyllum compared with alloxan alone treated group. Oxidative stress was noticed in rat’s pancreatic tissue as evidenced by a significant decrease in glutathione level, glutathione reductase, glutathione-S-transferase, and catalase activities. Malondialdehyde showed a significant increase compared to the normal saline-treated control group. Serum biochemistry and oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with L. microphyllum at a dose level of 100, 200 and 400 mg/kg body weight leaves extract for 14 d significantly prevented these alterations and attenuated alloxan-induced oxidative stress (P<0.05).Conclusion: The results of the present study indicated that the antihyperglycemic potential of L. microphyllum might be ascribable to its antioxidant and free radical scavenging properties. Thus, it is concluded that L. microphyllum may be helpful in the prevention of diabetic complications associated with oxidative stress.

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Oxidative stress markers and apoptosis in the prostate of diabetic rats and the influence of vitamin C treatment

Journal of Cellular Biochemistry ◽

10.1002/jcb.24092 ◽

2012 ◽

Vol 113 (7) ◽

pp. 2223-2233 ◽

Cited By ~ 10

Author(s):

Marina Guimarães Gobbo ◽

Daniele Lisboa Ribeiro ◽

Sebastião Roberto Taboga ◽

Eduardo Alves de Almeida ◽

Rejane Maira Góes

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Diabetic Rats ◽

Oxidative Stress Markers ◽

Stress Markers

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Safety Assessment of Artemether/Lumefantrine/ Tinidazole on the Kidneys of Healthy and Diseased Mice

Asian Journal of Biology ◽

10.9734/ajob/2021/v11i430150 ◽

2021 ◽

pp. 25-32

Author(s):

Elias Adikwu ◽

Udeme Owunari Georgewill

Keyword(s):

Oxidative Stress ◽

Safety Assessment ◽

Elevated Serum ◽

Oxidative Stress Markers ◽

Kidney Weight ◽

Protein Levels ◽

Tubular Necrosis ◽

Stress Markers ◽

Control Body

Artemether/lumefantrine/tinidazole- (A/L/T) can be use for the treatment of malaria; therefore its safety assessment is imperative. This study assessed its safety on the kidneys of healthy and diseased mice. Fifty four Swiss albino mice were used for this study. Mice were diseased with Plasmodium berghei () and treated with T (28.6 mg/kg), A/L (2.3/13.7mg/kg) and A/L/T for 4 days. Healthy mice were treated with T (28.6 mg/kg), A/L (2.3/13.7mg/kg) and A/L/T for 28 days. At the termination of treatment, the mice were weighed, sacrificed and blood samples were collected and examined for kidney biochemical markers. Kidneys were weighed and evaluated for oxidative stress markers and histology. T, A/L and A/L/T had no significant (p>0.05) effects on all evaluated parameters in diseased mice when compared to control. Body weight was decreased whereas kidney weight was increased in healthy mice treated with T, (p<0.05), A/L (p<0.05) and A/L/T (p<0.01) when compared to control. Significantly elevated serum creatinine, urea, uric acid levels with significantly decreased albumin, and total protein levels occurred in healthy mice treated with T (p<0.05), A/L (p<0.01) and A/L/T (p<0.001) when compared to control. Altered kidney oxidative stress markers characterized by significantly decreased glutathione, catalase, glutathione peroxidase, superoxide dismutase levels with significantly increased malondialdehyde levels occurred in healthy mice treated with T (p<0.05), A/L (p<0.01) and A/L/T (p<0.001) when compared to control. A/L/T produced tubular necrosis and enlarged Bowman’s space in healthy mice. The use of A/L/T as an antimalarial drug may be safe on the kidney, but long term use may cause kidney damage.

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Hepatoprotective Activities of Ethanolic Roots Extract of Ageratum Conyzoides on Alloxan-Induced Hepatic Damage in Diabetic Wistar Rats

Journal of Morphological Sciences ◽

10.1055/s-0038-1669423 ◽

2018 ◽

Vol 36 (01) ◽

pp. 039-045 ◽

Cited By ~ 2

Author(s):

A.O. Ojewale ◽

H.B. Akpan ◽

F.A. Faduyile ◽

P.D. Shallie ◽

A.A. Akande ◽

...

Keyword(s):

Oxidative Stress ◽

Blood Glucose ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Wistar Rats ◽

Diabetic Rats ◽

Hepatic Damage ◽

Oxidative Stress Markers ◽

Ageratum Conyzoides ◽

Stress Markers

Introduction The aim of the present study was to evaluate the hepatoprotective activities of the ethanolic roots extract of Ageratum conyzoides (AC) in alloxan-induced hepatic damage in diabetic rats. Materials and Methods Diabetes was induced in Wistar rats by the administration of alloxan (150 mg/kg, intraperitoneal). The ethanolic roots extract of AC, at doses of 250 and 500 mg/kg of body weight, was administered to diabetes-induced rats at a single dose per day for a period of 28 days. Results The effect of the ethanolic roots extract of AC on blood glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and hepatic oxidative stress markers was measured in the diabetic rats. The ethanolic roots extract of AC exhibited significant reduction of blood glucose (p < 0.05) at the dose of 500 mg/kg when compared with the standard drug glibenclamide (600 µg/kg). The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels increased significantly (p < 0.05) in the diabetic group without treatment when compared with the control group. In addition, the levels of oxidative stress markers, such as superoxide dismutase (SOD), catalase (CT), glutathione peroxidase (GPx), and glutathione (GSH), were significantly decreased in the diabetic rats compared with the normal rats, while the lipid peroxidation significantly increased in the diabetic group without treatment compared with the control (normal) group. The results demonstrated that the morphological, functional and oxidative stress changes in the liver caused by the ingestion of alloxan were attenuated in diabetic rats treated with the ethanolic roots extract of AC. Conclusion We concluded that the ethanolic roots extract of AC possesses significant antidiabetic, antioxidant and hepatoprotective effects on alloxan-induced diabetic rats.

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