scholarly journals Study of the potential synergistic antibacterial activity of essential oil components using the thiazolyl blue tetrazolium bromide (MTT) assay

LWT ◽  
2019 ◽  
Vol 101 ◽  
pp. 183-190 ◽  
Author(s):  
Raquel Requena ◽  
María Vargas ◽  
Amparo Chiralt
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Mtt Assay ◽  
Tetrazolium Bromide ◽  
Blue Tetrazolium ◽  
Thiazolyl Blue Tetrazolium Bromide ◽  
Oil Components ◽  
Thiazolyl Blue Tetrazolium ◽  
Synergistic Antibacterial Activity

A new assay for diaphorase activity in reagent formulations, based on the reduction of thiazolyl blue.

1979 ◽  
Vol 25 (12) ◽  
pp. 2040-2042 ◽  
Author(s):  
R S Boethling ◽  
T L Weaver
Keyword(s):  
Nonlinear Kinetics ◽  
Tetrazolium Bromide ◽  
Diaphorase Activity ◽  
Blue Tetrazolium ◽  
Maximum Absorbance ◽  
Thiazolyl Blue Tetrazolium Bromide ◽  
Assay Procedure ◽  
Thiazolyl Blue Tetrazolium

Abstract This new assay procedure for diaphorase eliminates problems of high blank rates and nonlinear kinetics associated with other methods. The dye thiazolyl blue tetrazolium bromide is reduced in the presence of NADH and diaphorase to yield a colored formazan, which as maximum absorbance at 560 nm.

scholarly journals ANTIBACTERIAL ACTIVITY OF AGASTACHE AURANTIACA ESSENTIAL OILS

2017 ◽  
pp. 63-70 ◽  
Author(s):  
Наталья (Natal'ja) Александровна (Aleksandrovna) Коваленко (Kovalenko) ◽  
Галина (Galina) Николаевна (Nikolaevna) Супиченко (Supichenko) ◽  
Татьяна (Tat'jana) Игоревна (Igorevna) Ахрамович (Ahramovich) ◽  
Анна (Anna) Геннадьевна (Gennad'evna) Шутова (Shutova) ◽  
Виктор (Viktor) Николаевич (Nikolaevich) Леонтьев (Leontiev)
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Properties ◽  
Gas Liquid Chromatography ◽  
Oil Components ◽  
The Republic ◽  
Main Components

The essential oil obtained by hydrodistillation method from plants of three varieties Agastache aurantiaca, cultivated on the Republic of Belarus were investigated. Some morphobiometric parameters of plants 'Tango', 'Apricot Sprite' and 'Fragrant Delight' and oil yields were determined. Using the technique of gas-liquid chromatography essential oil components were identified and determined. The main components of the essential oil from 'Tango' and 'Fragrant Delight' plants were menton (~ 53 and ~ 65 v/v % respectively) and pulegon (~ 36 and ~ 25 v/v % respectively). The samples of 'Apricot' Sprite' essential oil were rich in isomentone (~ 46 v/v %) and pulegon (~ 41 v/v %). All tested essential oils contained (+)-menton and (+)-pulegon enantiomeric forms only. The antibacterial activity of dimethylsulfoxide and ethanolic essential oil solutions against Staphylococcus aureus, Salmonella alony, Bacillus subtilis, Clostridium sp., Escherichia coli Hfr H, Pseudomonas aeruginosa was proved. Ethanol solutions of essential oils at the concentrations 0.001–0.1 v/v % had significant bactericidal activity. A correlation between the composition of the essential oil and their antibacterial properties was established.

Antibacterial activity of essential oil components

1992 ◽  
Vol 16 (4) ◽  
pp. 337-342 ◽  
Author(s):  
Venkataramana Moleyar ◽  
Pattisapu Narasimham
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Oil Components

scholarly journals Synergistic Antibacterial Activity of the Essential Oil of Aguaribay (Schinus molle L.)

Molecules ◽  
2012 ◽  
Vol 17 (10) ◽  
pp. 12023-12036 ◽  
Author(s):  
Pedro Rocha ◽  
Jesus Rodilla ◽  
David Díez ◽  
Heriberto Elder ◽  
Maria Guala ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Schinus Molle ◽  
Synergistic Antibacterial Activity

scholarly journals GC/GC-MS Analysis, Isolation and Identification of Bioactive Essential Oil Components from the Bhutanese Medicinal Plant, Pleurospermum Amabile

2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Phurpa Wangchuk ◽  
Paul A. Keller ◽  
Stephen G. Pyne ◽  
Malai Taweechotipatr ◽  
Sumalee Kamchonwongpaisan
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Medicinal Plant ◽  
Multidrug Resistant ◽  
Methyl Eugenol ◽  
Nmr Analysis ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Oil Components

We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with ( E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; ( E)-isomyristicin (1), ( E)-isoapiol (2), methyl eugenol (3) and ( E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the test samples showed cytotoxicity.

Antibacterial Activity of Essential Oil Components and Their Potential Use in Seed Disinfection

2009 ◽  
Vol 57 (20) ◽  
pp. 9454-9461 ◽  
Author(s):  
Pietro Lo Cantore ◽  
Vellasamy Shanmugaiah ◽  
Nicola Sante Iacobellis
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Seed Disinfection ◽  
Oil Components ◽  
Potential Use
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