scholarly journals Detection of durum wheat pasta adulteration with common wheat by infrared spectroscopy and chemometrics: A case study

LWT ◽  
2020 ◽  
Vol 127 ◽  
pp. 109368 ◽  
Author(s):  
Annalisa De Girolamo ◽  
Marcia Carolina Arroyo ◽  
Salvatore Cervellieri ◽  
Marina Cortese ◽  
Michelangelo Pascale ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Durum Wheat ◽  
Common Wheat

Effect of algae Ecklonia maxima extract (Kelpak SL) on yields of common wheat, durum wheat and spelt wheat

2019 ◽  
Vol 74 (1) ◽  
pp. 5-14
Author(s):  
GRZEGORZ SZUMIŁO ◽  
LESZEK RACHOŃ ◽  
BARBARA KROCHMAL-MARCZAK
Keyword(s):  
Triticum Aestivum ◽  
Durum Wheat ◽  
Common Wheat ◽  
Yield Components ◽  
Grain Quality ◽  
Foliar Application ◽  
Control Treatment ◽  
Favourable Effect ◽  
Ecklonia Maxima ◽  
Spelt Wheat

The 3-year experiment was concerned with the response of spring forms of common wheat (Triticum aestivum L. subsp. aestivum), durum wheat (Triticum durum Desf.) and spelt wheat (Triticum aestivum subsp. spelta L. em. Thell.) to the foliar application of a plant growth stimulant (extract from marine algae Ecklonia maxima), with the commercial name of Kelpak SL (GS), as compared to control treatment (C). The following parameters were analysed: yield of grain, yield components (number of ears, weight of 1000 kernels, number and weight of kernels per ear) and physical indicators of grain quality (test weight, uniformity and vitreosity of grain). The study showed that the level of yielding and the yield components were related primarily with the wheat genotype, but they depended also on the agro-climatic conditions and on the algae extract and control experimental treatments. The application of algae extract, compared to the control, caused a significant increase in the yields of the spring wheat species under study, on average by 7.0%. Canopy spraying with algae extract had a favourable effect on the number of ears, on he number and weight of kernels per ear, but it had no effect on the weight of 1000 kernels. The grain quality of durum wheat, spelt wheat and common wheat was affected more strongly by the weather conditions in the successive years of the study and by the genotype than by the foliar application of algae extract. The spelt genotypes were characterised by lower yields and lower grain quality than common wheat and the durum wheat genotypes.

scholarly journals Development and validation of a method for quantification of common wheat, durum wheat, rye and barley by droplet digital PCR

2021 ◽  
Author(s):  
Christian Schulze ◽  
Anne-Catrin Geuthner ◽  
Dietrich Mäde
Keyword(s):  
Durum Wheat ◽  
Real Time ◽  
Bread Wheat ◽  
Common Wheat ◽  
Real Time Pcr ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Food Fraud ◽  
Single Genome ◽  
Cereal Species

AbstractFood fraud is becoming a prominent topic in the food industry. Thus, valid methods for detecting potential adulterations are necessary to identify instances of food fraud in cereal products, a significant component of human diet. In this work, primer–probe systems for real-time PCR and droplet digital PCR (ddPCR) for the detection of these cereal species: bread wheat (together with spelt), durum wheat, rye and barley for real-time PCR and ddPCR were established, optimized and validated. In addition, it was projected to validate a molecular system for differentiation of bread wheat and spelt; however, attempts for molecular differentiation between common wheat and spelt based on the gene GAG56D failed because of the genetic variability of the molecular target. Primer–probe systems were further developed and optimized on the basis of alignments of DNA sequences, as well as already developed PCR systems. The specificity of each system was demonstrated on 10 (spelt), 11 (durum wheat and rye) and 12 (bread wheat) reference samples. Specificity of the barley system was already proved in previous work. The calculated limits of detection (LOD95%) were between 2.43 and 4.07 single genome copies in real-time PCR. Based on the “three droplet rule”, the LOD95% in ddPCR was calculated to be 9.07–13.26 single genome copies. The systems were tested in mixtures of flours (rye and common wheat) and of semolina (durum and common wheat). The methods proved to be robust with regard to the tested conditions in the ddPCR. The developed primer–probe systems for ddPCR proved to be effective in quantitatively detecting the investigated cereal species rye and common wheat in mixtures by taking into account the haploid genome weight and the degree of milling of a flour. This method can correctly detect proportions of 50%, 60% and 90% wholemeal rye flour in a mixture of wholemeal common wheat flour. Quantitative results depend on the DNA content, on ploidy of cereal species and are also influenced by comminution. Hence, the proportion of less processed rye is overestimated in higher processed bread wheat and adulteration of durum wheat by common wheat by 1–5% resulted in underestimation of common wheat.

Quantification of Common Wheat Adulteration of Durum Wheat Pasta Using Real-Time Quantitative Polymerase Chain Reaction (PCR)

2002 ◽  
Vol 79 (4) ◽  
pp. 553-558 ◽  
Author(s):  
Rémi Alary ◽  
Arnaud Serin ◽  
Marie-Pierre Duviau ◽  
Philippe Jourdrier ◽  
Marie-Françoise Gautier
Keyword(s):  
Polymerase Chain Reaction ◽  
Durum Wheat ◽  
Real Time ◽  
Common Wheat ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Virulence Phenotypes and Molecular Genotypes in Collections of Puccinia triticina from Italy

Plant Disease ◽  
2010 ◽  
Vol 94 (4) ◽  
pp. 420-424 ◽  
Author(s):  
Paola Mantovani ◽  
Marco Maccaferri ◽  
Roberto Tuberosa ◽  
James Kolmer
Keyword(s):  
Durum Wheat ◽  
Common Wheat ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Leaf Rust Resistance Gene ◽  
The Third ◽  
Virulence Phenotype ◽  
Ssr Loci ◽  
Highly Correlated ◽  
Simple Sequence

Twenty-four isolates of Puccinia triticina from Italy were characterized for virulence to seedlings of 22 common wheat Thatcher isolines, each with a different leaf rust resistance gene, and for molecular genotypes at 15 simple sequence repeat (SSR) loci. The isolates were compared to a set of 13 previously characterized P. triticina isolates from either durum or common wheat. Clustering based on virulence phenotypes and SSR genotypes grouped the Italian P. triticina isolates into three groups. In the first group, the isolates had virulence phenotypes and SSR genotypes that were similar to the isolates collected from durum wheat. Isolates in the second group were unique because they had virulence similar to the isolates from common wheat but were distinct for SSR genotypes compared to the isolates from durum wheat and from common wheat. Isolates in the third group had virulence phenotypes and SSR genotypes closely related to the isolates from common wheat. The isolates were grouped based on the known or assumed host of origin, virulence phenotype, and SSR genotypes. Measures of FST and RST for SSR genotypes, and ΦST for virulence phenotype were significant, which indicated differentiation among the three groups of isolates. Virulence phenotypes and molecular genotypes were highly correlated with r = 0.74.

Triticum polonicum L. as potential source material for the biofortification of wheat with essential micronutrients

2018 ◽  
Vol 17 (03) ◽  
pp. 213-220 ◽  
Author(s):  
Teresa Bieńkowska ◽  
Elżbieta Suchowilska ◽  
Wolfgang Kandler ◽  
Rudolf Krska ◽  
Marian Wiwart
Keyword(s):  
Durum Wheat ◽  
Common Wheat ◽  
Principal Component ◽  
Small Scale ◽  
Wheat Varieties ◽  
Triticum Polonicum ◽  
Iron And Zinc ◽  
Modern Wheat ◽  
Wheat Lines ◽  
Source Materials

AbstractThe grain of modern wheat cultivars has a significantly lower mineral content, including the content of copper, iron, magnesium, manganese, phosphorous, selenium and zinc. For this reason cereal breeders, are constantly searching for new genetic sources of minerals that are essential in human nutrition. Triticum polonicum, which is grown on a small scale in Spain, southern Italy, Algeria, Ethiopia and warm regions of Asia, deserves special attention in this context. The micronutrient and macronutrient content of T. polonicum versus T. durum and T. aestivum was compared in this study. Polish wheat grain was characterized by the significantly highest content of phosphorus (4.55 g/kg), sulphur (1.82 g/kg), magnesium (1.42 g/kg), zinc (49.5 mg/kg), iron (39.1 mg/kg) and boron (0.56 mg/kg) as well as a low content of aluminium (only 1.04 mg/kg). The macronutrient profile of most T. polonicum lines differed completely from that of common wheat and durum wheat. The principal component analysis supported discrimination of seven Polish wheat lines with a particularly beneficial micronutrient profile (P2, P3, P5, P7, P9, P22 and P25). These lines were characterized by the highest content of copper, iron and zinc, as well as the lowest concentrations of strontium, aluminium and barium which are undesirable in food products. The above lines can be potentially applied as source materials for breeding new wheat varieties. The results of this study indicate that Polish wheat could be used in genetic biofortification of durum wheat and common wheat.

scholarly journals The occurrence of fungi on roots and stem bases of common wheat (Triticum aestivum ssp. vulgare L.) and durum wheat (Triticum durum Desf.) grown under two levels of chemical protection

2012 ◽  
Vol 64 (3) ◽  
pp. 93-102
Author(s):  
Irena Kiecana ◽  
Leszek Rachoń ◽  
Elżbieta Mielniczuk ◽  
Grzegorz Szumiło
Keyword(s):  
Durum Wheat ◽  
Common Wheat ◽  
Triticum Durum ◽  
Experimental Treatment ◽  
Bipolaris Sorokiniana ◽  
Wheat Roots ◽  
Chemical Protection ◽  
The Mean ◽  
Triticum Durum Desf ◽  
The University

Investigations were carried out in 2007-2009 on the plots of the Felin Experimental Station belonging to the University of Life Science in Lublin. The studies comprised two cultivation lines of durum wheat (<i>Triticum durum</i> L.): STH 716 and STH 717, as well as the 'Tonacja' cultivar of common wheat (<i>T. aestivum</i> ssp. <i>vulgare</i> L.). Two levels of chemical protection were applied in the cultivation: minimal and complex protection. Infection of wheat roots and stem bases was recorded in each growing season at hard dough stage (87 in Tottman's scale, 1987). After three years of study, the mean disease indexes for the analyzed wheat genotypes in the experimental treatment with minimal protection were 31.13, 30.43 and 38.83 for, respectively, the 'Tonacja' cultivar and the cultivation lines of <i>T. durum</i> STH 716 and STH 717. In the experimental combination with complex protection, after three years of study the disease indexes ranged from 25.26 (<i>T. durum</i> STH 716) to 30.83 (<i>T. durum</i> STH 717). The results of mycological analysis of diseased plants showed that <i>Fusarium</i> spp., especially <i>F. culmorum</i>, <i>F. avenaceum</i> as well as <i>Bipolaris sorokiniana</i> and <i>Rhizoctonia solani</i>, caused root rot and necrosis of wheat stem bases. The analyzed chemical protection levels did not significantly influence grain yield of the investigated genotypes of <i>T. aestivum</i> and <i>T. durum</i>.

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