Synthesis and characterisation of alternan-stabilised silver nanoparticles and determination of their antibacterial and antifungal activities against foodborne pathogens and fungi

LWT ◽  
2020 ◽  
Vol 128 ◽  
pp. 109497 ◽  
Author(s):  
Mustafa Tahsin Yilmaz ◽  
Hümeyra İspirli ◽  
Osman Taylan ◽  
Enes Dertli
PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0241729
Author(s):  
Parameshwar Jakinala ◽  
Nageshwar Lingampally ◽  
Bee Hameeda ◽  
R. Z. Sayyed ◽  
Yahya Khan M. ◽  
...  

Silver nanoparticles (AgNPs) are among the most widely synthesized and used nanoparticles (NPs). AgNPs have been traditionally synthesized from plant extracts, cobwebs, microorganisms, etc. However, their synthesis from wing extracts of common insect; Mang mao which is abundantly available in most of the Asian countries has not been explored yet. We report the synthesis of AgNPs from M. mao wings extract and its antioxidant and antimicrobial activity. The synthesized AgNPs were spherical, 40–60 nm in size and revealed strong absorption plasmon band around at 430 nm. Highly crystalline nature of these particles as determined by Energy-dispersive X-ray analysis and X-ray diffraction further confirmed the presence of AgNPs. Hydrodynamic size and zeta potential of AgNPs were observed to be 43.9 nm and -7.12 mV, respectively. Fourier-transform infrared spectroscopy analysis revealed the presence of characteristic amide proteins and aromatic functional groups. Thin-layer chromatography (TLC) and Gas chromatography-mass spectroscopy (GC-MS) analysis revealed the presence of fatty acids in the wings extract that may be responsible for biosynthesis and stabilization of AgNPs. Further, SDS-PAGE of the insect wing extract protein showed the molecular weight of 49 kDa. M. mao silver nanoparticles (MMAgNPs) exhibit strong antioxidant, broad-range antibacterial and antifungal activities, (66.8 to 87.0%), broad-range antibacterial and antifungal activities was found with maximum zone of inhibition against Staphylococcus aureus MTCC 96 (35±0.4 mm) and Fusarium oxysporum f. sp. ricini (86.6±0.4) which signifies their biomedical and agricultural potential.


2019 ◽  
Vol 9 (1-s) ◽  
pp. 84-87 ◽  
Author(s):  
Sapna Malviya ◽  
P. Dwivedi

Ailanthus excels belongs to family Simaroubaceae known as tree of Heaven and Mahanimba commonly found in the India and China. The present study involves preliminary screening, qualitative analysis and pharmacological evaluation of extract. The preliminary photochemical screening performed using petroleum ether, chloroform and methanol extract. Analytical techniques like; TLC, FTIR and HPTLC also performed for qualitative and quantitative determination. Various chemical tests also performed for qualitative determination of alkaloids, flavanoids, phenols, terpenoids, steroids and saponins in plant extracts. Plant extract finally subjected to antibacterial and antifungal activities and findings of study suggested that plant extracts possesses potent antibacterial and antifungal activities. Keywords: Ailanthus excelsa, antibacterial, antifungal, preliminary screening, TLC, HPTLC.


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