Biosynthesis of silver nanoparticles and its antibacterial and antifungal activities towards Gram-positive, Gram-negative bacterial strains and different species of Candida fungus

Abstract In this study the antibacterial and antifungal activities of extracted Achillea latiloba Ledeb. ex Nordm (Asteraceae) samples in acetone, ethyl acetate, ethanol, methanol and dimethyl sulfoxide (DMSO) from Trabzon Province (Turkey) were investigated. Antimicrobial activity of A. latiloba varied depending on the extract of samples, dosage of extracts, and the extraction solvents for all test microorganisms. Staphylococcus aureus, Streptococcus salivarius, Klebsiella pneumoniae, Escherichia coli, Salmonella enteritidis, Streptococcus pneumoniae, Bacillus cereus, Listeria monocytogenes, Streptococcus mutans, Pseudomonas aeruginosa, Bacillus licheniformis, Micrococcus luteus, Bacillus subtillis, Proteus vulgaris and Candida albicans were studied with use of disc diffusion and agar dilution method. The results indicated that each of the crude extracts of Achillea latiloba exhibited a more or less pronounced antibacterial and antifungal potency both in Gram-positive and Gram-negative bacteria and fungi. While in the Gram-negative group, the most sensitive microorganism to Achillea latiloba were S. enteritidis and Streptococcus mutans which is Gram-positive. In the Gram-positive group, the microorganisms most sensitive to Achillea latiloba were Streptococcus mutans and L. monocytogenes. However, the least sensitive microorganism was P. vulgaris. The results presented in this paper suggest that Achillea latiloba possesses additional antimicrobial activities that has an effect against some Gram-negative, Gram-positive bacteria and fungi.

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Anticancer and Antimicrobial Activity Evaluation of Cowpea-Porous-Starch-Formulated Silver Nanoparticles

Journal of Nanotechnology ◽

10.1155/2021/5525690 ◽

2021 ◽

Vol 2021 ◽

pp. 1-13

Author(s):

Shiara Ramdath ◽

John Mellem ◽

Londiwe Simphiwe Mbatha

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Cell Viability ◽

High Dose ◽

Bacterial Strains ◽

Gram Positive ◽

Gram Negative ◽

Anticancer Drug Delivery ◽

Ic50 Values ◽

Biosynthesized Agnps

Health issues involving inadequate treatment of diseases such as cancer and microbial infections continue to be the subject of much ongoing recent research. Biosynthesized silver nanoparticles (AgNPs) were characterized using Transmission Electron Microscopy (TEM), Zeta Sizer, Ultraviolet (UV), and Fourier Transform Infrared (FTIR) spectroscopy. Their antimicrobial activity was evaluated on selected Gram-positive and Gram-negative bacterial strains, using the disc diffusion and broth dilution assays. Cell viability profiles were evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and apoptosis studies on selected human noncancer and cancer cells. The biosynthesized AgNPs were evaluated to be spherical clusters, with sizes between 40 and 70 nm. The absorption peak at 423 nm and the presence of polyphenols confirmed the synthesis and stabilization of these tested AgNPs. The AgNPs showed a good stability of −23.9 ± 1.02 mV. Good antimicrobial activity (6.0–18.0 mm) was seen on all tested bacteria at a minimum inhibitory concentration (MIC) ranging from 5 to 16 μg/ml, with the highest activity seen against Gram-negative Escherichia coli (18 ± 0.5 mm), and the lowest activity was seen against Gram-positive Listeria monocytogenes (6.0 ± 0.4 mm) after treatment with the AgNPs. These NPs showed a concentration-dependent and cell-specific cytotoxicity with low IC50 values (41.7, 56.3, and 63.8 μg/ml). The NPs were well tolerated by tested cells as indicated by a more than 50% cell viability at the high dose tested and low apoptotic indices (<0.2). These findings indicated that these biosynthesized AgNPs showed great potential as effective antibacterial agents and anticancer drug delivery modalities.

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Mycosynthesizing and characterizing silver nanoparticles from the mushroom Inonotus hispidus (Hymenochaetaceae), and their antibacterial and antifungal activities

Environmental Nanotechnology Monitoring & Management ◽

10.1016/j.enmm.2020.100313 ◽

2020 ◽

Vol 14 ◽

pp. 100313

Author(s):

Ahmed Saadoon Jaloot ◽

Mustafa Nadhim Owaid ◽

Ghassan Adnan Naeem ◽

Rasim Farraj Muslim

Keyword(s):

Silver Nanoparticles ◽

Antifungal Activities ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal

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In VitroPhytochemical, Antibacterial, and Antifungal Activities of Leaf, Stem, and Root Extracts ofAdiantum capillus veneris

The Scientific World JOURNAL ◽

10.1155/2014/269793 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 20

Author(s):

Muhammad Saqib Ishaq ◽

Muhammad Medrar Hussain ◽

Muhammad Siddique Afridi ◽

Ghadir Ali ◽

Mahrukh Khattak ◽

...

Keyword(s):

Multidrug Resistant ◽

Antimicrobial Activities ◽

Phytochemical Analysis ◽

Bacterial Strains ◽

Antifungal Activities ◽

Fungal Strains ◽

Antibacterial And Antifungal Activities ◽

Adiantum Capillus Veneris ◽

Ethanol Extracts ◽

Antibacterial And Antifungal

Adiantum capillus venerisis a medicinally essential plant used for the treatment of diverse infectious diseases. The study of phytochemical and antimicrobial activities of the plant extracts against multidrug-resistant (MDR) bacteria and medically important fungi is of immense significance. Extracts from the leaves, stems, and roots ofAdiantum capillus veneriswere extracted with water, methanol, ethanol, ethyl acetate, and hexane and screened for their antimicrobial activity against ten MDR bacterial strains and five fungal strains isolated from clinical and water samples. Ash, moisture, and extractive values were determined according to standard protocols. FTIR (Fourier transform infrared Spectroscopy) studies were performed on different phytochemicals isolated from the extracts ofAdiantum capillus Veneris. Phytochemical analysis showed the presence of flavonoids, alkaloids, tannins, saponins, cardiac glycosides, terpenoids, steroids, and reducing sugars. Water, methanol, and ethanol extracts of leaves, stems, and roots showed significant antibacterial and antifungal activities against most of the MDR bacterial and fungal strains. This study concluded that extracts ofAdiantum capillus venerishave valuable phytochemicals and significant activities against most of the MDR bacterial strains and medically important fungal strains.

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Infrared, 1H-NMR Spectral Studies of some Methyl 6-O-Myristoyl-α-D-Glucopyranoside Derivatives: Assessment of Antimicrobial Effects

International Letters of Chemistry Physics and Astronomy ◽

10.18052/www.scipress.com/ilcpa.58.122 ◽

2015 ◽

Vol 58 ◽

pp. 122-136 ◽

Cited By ~ 4

Author(s):

Sarkar M.A. Kawsar ◽

Khaleda Mymona ◽

Refat Asma ◽

Mohammad A. Manchur ◽

Yasuhiro Koide ◽

...

Keyword(s):

Pathogenic Fungi ◽

Spectral Studies ◽

Bacterial Strains ◽

Substitution Product ◽

Antifungal Activities ◽

H Nmr ◽

Antibacterial And Antifungal Activities ◽

Fungal Phytopathogens ◽

Antibacterial And Antifungal

This study was carried out to regioselective myristoylation of methyl α-D-glucopyranoside (1) using the direct acylation method gave the corresponding methyl 6-O-myristoyl-α-D-glucopyranoside (2) in fair yield. A number of 2,3,4-tri-O-acyl derivatives (3-15) of this 6-O-substitution product using a wide variety of acylating agents were also prepared in order to obtain newer derivatives of synthetic and biological importance. The reaction conditions are reasonably simple and yields were very good. The structures of the title compounds (2-15) were established by using analytical, physicochemical techniques and spectroscopic data (IR and 1H-NMR). All the synthesized compounds were employed as test chemicals for in vitro antimicrobial functionality test against Gram-positive Bacillus subtilis, Staphylococcus aureus, Gram-negative Escherichia coli, Pseudomonas aeruginosa bacteria and plant pathogenic fungi Aspergillus niger and Candida albicans. For comparative studies, antimicrobial activity of standard antibiotics, Ampicillin and Nystatin were also carried out against these microorganisms. The study revealed that the tested samples exhibited moderate to good antibacterial and antifungal activities. It was also observed that the test substances were more effective against fungal phytopathogens than those of the human bacterial strains. Encouragingly, a number of tested chemicals showed nearest antibacterial and antifungal activities with the standard antibiotics employed.

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Comprehensive Evaluation of the Antibacterial and Antifungal Activities of Carlina acaulis L. Essential Oil and Its Nanoemulsion

Antibiotics ◽

10.3390/antibiotics10121451 ◽

2021 ◽

Vol 10 (12) ◽

pp. 1451

Author(s):

Antonio Rosato ◽

Alexia Barbarossa ◽

Ahmed M. Mustafa ◽

Giulia Bonacucina ◽

Diego Romano Perinelli ◽

...

Keyword(s):

Essential Oil ◽

Comprehensive Evaluation ◽

Antimicrobial Properties ◽

Homogenization Method ◽

Bacterial Strains ◽

Antifungal Activities ◽

Fungal Strains ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal ◽

New Compounds

Plants are considered to be an excellent source of new compounds with antibiotic activity. Carlina acaulis L. is a medicinal plant whose essential oil (EO) is mainly characterized by the polyacetylene carlina oxide, which has antimicrobial properties. The aim of this study was to evaluate the antimicrobial and antifungal activities of C. acaulis EO, carlina oxide, and nanoemulsion (NE) containing the EO. The EO was obtained through plant roots hydrodistillation, and carlina oxide was purified from it through silica gel column chromatography. The NE containing C. acaulis EO was prepared with the high-pressure homogenization method, and the minimum inhibitory concentration (MIC) was determined against several bacterial and fungal strains for all the C. acaulis-derived products. The latter resulted in activity active versus all the screened Gram-positive bacterial strains and also on all the fungal strains with low MIC values. For yeast, the EO and carlina oxide showed good MIC values. The EO-NE demonstrated a better activity than the pure EO on all the tested bacterial and fungal strains. The results suggest that C. acaulis-derived products could be potential candidates for the development of natural antibacterial and antifungal agents.

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Antibacterial and Antifungal Activities of Rumex Confertus Willd

International Journal for Research in Applied Science and Engineering Technology ◽

10.22214/ijraset.2021.39666 ◽

2021 ◽

Vol 9 (12) ◽

pp. 855-856

Author(s):

G. D. Shermatova

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Gram Positive ◽

Antifungal Activities ◽

Gram Positive Bacteria ◽

Root Part ◽

Fungal Activity ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal

Abstract: The leaves and roots of Rumex confertus Willd were tested in vitro for antibacterial and fungal activity in the fractions of gasoline, chloroform, ethyl acetate and butanol. As a result, it was found that the leaves of the Rumex confertus Willd plant, chloroform and ethyl acetate fractions of the root part have antibacterial activity against fungi and gram-positive bacteria. Keywords: Rumex confertus Willd, fractions, extracts, antibacterial, antifungal

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Sequential multicomponent catalytic synthesis of pyrrole-3-carboxaldehydes: evaluation of antibacterial and antifungal activities along with docking studies

New Journal of Chemistry ◽

10.1039/d0nj03575k ◽

2020 ◽

Vol 44 (38) ◽

pp. 16329-16339

Author(s):

Nisar A. Mir ◽

Panduga Ramaraju ◽

Satheeshvarma Vanaparthi ◽

Sachin Choudhary ◽

Rajnish P. Singh ◽

...

Keyword(s):

Docking Studies ◽

Catalytic Synthesis ◽

Bacterial Strains ◽

Antifungal Activities ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal

A sequential multicomponent method is developed to access highly substituted N-arylpyrrole-3-carbaldehydes and tested for antibacterial and antifungal activities against bacterial strains.

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Evaluation of Benzophenone-N-ethyl Morpholine Ethers as Antibacterial and Antifungal Activities

Journal of Chemistry ◽

10.1155/2014/941074 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

A. Bushra Begum ◽

Noor Fatima Khanum ◽

V. Lakshmi Ranganatha ◽

T. Prashanth ◽

Mohammed Al-Ghorbani ◽

...

Keyword(s):

Bacterial Infections ◽

Human Beings ◽

Bacterial Strains ◽

Chemical Methods ◽

Subtle Change ◽

Antifungal Activities ◽

Antibacterial And Antifungal Activities ◽

Paratyphi B ◽

Antibacterial And Antifungal ◽

Microbiological Assays

Microorganisms are closely associated with the health and welfare of human beings. Whereas some microorganisms are beneficial, others are detrimental. Bacterial infections often produce inflammation and pains and in some instances, infections result in high mortality. Any subtle change in the drug molecule, which may not be detected by chemical methods, can be revealed by a change in the antimicrobial activity and hence microbiological assays are very useful. A series of substituted hydroxy benzophenones and benzophenone-N-ethyl morpholine ethers were screened for their antibacterial and antifungal activities. Antibacterial activity againstS. aureus,E. aerogenes,M. luteus,K. pneumonia, andS. typhimurium,S. paratyphi-BandP. vulgarisbacterial strains and antifungal activity againstC. albicans,B. cinerea,M. pachydermatis,C. kruseifungal strains were carried out. The bioassays indicated that most of the synthesized compounds showed potential antibacterial and antifungal agents.

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