scholarly journals Development of three-dimensional piezoelectric polyvinylidene fluoride-graphene oxide scaffold by non-solvent induced phase separation method for nerve tissue engineering

2019 ◽  
Vol 167 ◽  
pp. 107636 ◽  
Author(s):  
Nadia Abzan ◽  
Mahshid Kharaziha ◽  
Sheyda Labbaf
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Phase Separation ◽  
Polyvinylidene Fluoride ◽  
Three Dimensional ◽  
Separation Method ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering

Enhancing neuronal growth from human endometrial stem cells derived neuron-like cells in three-dimensional fibrin gel for nerve tissue engineering

2013 ◽  
Vol 102 (8) ◽  
pp. 2533-2543 ◽  
Author(s):  
Mona Navaei-Nigjeh ◽  
Ghasem Amoabedini ◽  
Abbas Noroozi ◽  
Mahmoud Azami ◽  
Mohammad N. Asmani ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Three Dimensional ◽  
Nerve Tissue ◽  
Neuronal Growth ◽  
Fibrin Gel ◽  
Endometrial Stem Cells ◽  
Nerve Tissue Engineering

scholarly journals Indirect 3D bioprinting and characterization of alginate scaffolds for potential nerve tissue engineering applications

2019 ◽  
Author(s):  
Saman Naghieh ◽  
Md Sarker ◽  
Emily Abelseth ◽  
Xiongbiao Chen
Keyword(s):  
Tissue Engineering ◽  
Effective Means ◽  
Three Dimensional ◽  
Point Of View ◽  
Nerve Tissue ◽  
Biological Assessment ◽  
Engineering Applications ◽  
Low Concentration ◽  
Cell Functions ◽  
Nerve Tissue Engineering

Low-concentration hydrogels have favorable properties for many cell functions in tissue engineering but are considerably limited from a scaffold fabrication point of view due to poor three-dimensional (3D) printability. Here, we developed an indirect-bioprinting process for alginate scaffolds and characterized the potential of these scaffolds for nerve tissue engineering applications. The indirect-bioprinting process involves (1) printing a sacrificial framework from gelatin, (2) impregnating the framework with low-concentration alginate, and (3) removing the gelatin framework by an incubation process, thus forming low-concentration alginate scaffolds. The scaffolds were characterized by compression testing, swelling, degradation, and morphological and biological assessment of incorporated or seeded Schwann cells. For comparison, varying concentrations of alginate scaffolds (from 0.5 to 3%) were fabricated and sterilized using either ultraviolet light or ethanol. Results indicated that scaffolds can be fabricated using the indirect-bioprinting process, wherein the scaffold properties are affected by the concentration of alginate and sterilization technique used. These factors provide effective means of regulating the properties of scaffolds fabricated using the indirect-bioprinting process. Cell-incorporated scaffolds demonstrated better cell viability than bulk gels. In addition, scaffolds showed better cell functionality when fabricated with a lower concentration of alginate compared to a higher concentration. The indirect-bioprinting process that we implemented could be extended to other types of low-concentration hydrogels to address the tradeoffs between printability and properties for favorable cell functions.

scholarly journals Fabrication of Graphene Oxide Aerogel to Repair Neural Tissue

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Khadijeh Zeinali ◽  
Mohammad Taghi Khorasani ◽  
Alimorad Rashidi ◽  
Morteza Daliri Jouparid
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Three Dimensional ◽  
Neural Tissue ◽  
Nerve Tissue ◽  
Neural Tissue Engineering ◽  
Tissue Scaffold ◽  
Reduced Graphene ◽  
Graphene Oxide Aerogel

: The neural tissue engineering has been designed as a subset of tissue engineering for treating congenital malformations and accident injuries, particularly for individuals requiring tissue grafting. Such transplants, usually performed as autografting, can often not meet the requirements of an effective scaffold used in nerve tissue engineering. A novel neural tissue scaffold was introduced here to solve the problem concerning the reduced graphene oxide. The three-dimensional graphene oxide in the neural canal restricts the formation of fibroglandular tissues and facilities neural stem cell proliferation and growth. In these techniques, graphene oxide aerogel was initially made. Then, the freeze-drying process was used to fix the geometry of reduced graphene oxide hydrogels prepared using graphene oxide dispersion and ethylenediamine and gain aerogels. The X-ray diffraction patterns, FTIR and morphological related to samples were examined, followed by conducting in-vitro micropropagation and 4, 6-diamidino-2-phenylindol (DAPI) staining in fibroblast and P19 cultures. The results from immunofluorescence staining demonstrated the neural differentiation of P19 cells. It can be concluded that most cells attached to and differentiated on the scaffold surface and axons can penetrate randomly through them. Finally, the three-dimensional graphene oxide was proposed as an ideal alternative to be used in neural tissue engineering.

scholarly journals Tailoring Nano-Porous Surface of Aligned Electrospun Poly (L-Lactic Acid) Fibers for Nerve Tissue Engineering

2021 ◽  
Vol 22 (7) ◽  
pp. 3536
Author(s):  
Hongyun Xuan ◽  
Biyun Li ◽  
Feng Xiong ◽  
Shuyuan Wu ◽  
Zhuojun Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Bacterial Adhesion ◽  
Bacterial Colonization ◽  
Cellular Responses ◽  
Surface Structures ◽  
Porous Surface ◽  
Nerve Tissue ◽  
Proliferation And Differentiation ◽  
Nerve Tissue Engineering

Despite the existence of many attempts at nerve tissue engineering, there is no ideal strategy to date for effectively treating defective peripheral nerve tissue. In the present study, well-aligned poly (L-lactic acid) (PLLA) nanofibers with varied nano-porous surface structures were designed within different ambient humidity levels using the stable jet electrospinning (SJES) technique. Nanofibers have the capacity to inhibit bacterial adhesion, especially with respect to Staphylococcus aureus (S. aureus). It was noteworthy to find that the large nano-porous fibers were less detrimentally affected by S. aureus than smaller fibers. Large nano-pores furthermore proved more conducive to the proliferation and differentiation of neural stem cells (NSCs), while small nano-pores were more beneficial to NSC migration. Thus, this study concluded that well-aligned fibers with varied nano-porous surface structures could reduce bacterial colonization and enhance cellular responses, which could be used as promising material in tissue engineering, especially for neuro-regeneration.

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