Preparation of silk fibroin/collagen/hydroxyapatite composite scaffold by particulate leaching method

2013 ◽  
Vol 105 ◽  
pp. 189-191 ◽  
Author(s):  
Zhao-Li Mou ◽  
Li-Min Duan ◽  
Xiao-Ni Qi ◽  
Zhi-Qi Zhang
Keyword(s):  
Silk Fibroin ◽  
Composite Scaffold ◽  
Hydroxyapatite Composite ◽  
Particulate Leaching

scholarly journals Mechanical properties of Gelatin –Hydroxyapatite composite for bone tissue engineering

2015 ◽  
Vol 50 (1) ◽  
pp. 15-20 ◽  
Author(s):  
MJ Hossan ◽  
MA Gafur ◽  
MM Karim ◽  
AA Rana
Keyword(s):  
Mechanical Properties ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Testing Machine ◽  
Casting Process ◽  
Raw Material ◽  
Potential Candidate ◽  
Oven Drying ◽  
Hydroxyapatite Composite ◽  
Properties Of Composites

In this study, hydroxyapatite (HAp) and gelatin (GEL) scaffolds were prepared to mimic the mineral and organic component of natural bone. The raw material was first compounded and resulting composite were molded into the petridishes. Using Solvent casting process, it is possible to produce scaffolds with mechanical and structural properties close to natural trabecular bone.The mechanical properties of composites were investigated by Thermo-mechanical analyzer (TMA), Vickers microhardness tester, Universal testing machine. It was observed that the composite has maximum tensile strength of 37.13MPa ( oven drying) and % elongation of 7.68 (Oven drying) and 2.04 (Natural drying) at 15% of Hap respectively. These results demonstrate that the prepared composite scaffold is a potential candidate for bone tissue engineering.Bangladesh J. Sci. Ind. Res. 50(1), 15-20, 2015

scholarly journals Silk fibroin hydroxyapatite composite thermal stabilisation of carbonic anhydrase

2016 ◽  
Vol 4 ◽  
Author(s):  
Zhang Zishuai ◽  
Lopes Joao ◽  
Marelli Benedetto ◽  
Omenetto Forenzo ◽  
Kaplan David ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Silk Fibroin ◽  
Hydroxyapatite Composite

Design of a Collagen/Silk Mechano-Compatible Composite Scaffold for the Vascular Tissue Engineering: Focus on Compliance

2007 ◽  
Vol 334-335 ◽  
pp. 1169-1172 ◽  
Author(s):  
Frédéric Couet ◽  
Navneeta Rajan ◽  
Simone Vesentini ◽  
D. Mantovani
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Vascular Tissue ◽  
Composite Scaffold ◽  
Collagen Gel ◽  
Element Model ◽  
Natural Fibre ◽  
Filament Winding ◽  
Laminate Theory

One of the merging methods to produce tissue-engineered vascular substitutes is to process scaffolds to direct the regeneration of vascular tissues. Collagen, as one of the main protein in the vascular extracellular matrix, is one of biopolymers that exhibits a major potential for scaffold technology. However, gels made from reconstituted collagen generally exhibit poor mechanical properties and limited manipulability. Therefore, adding a reinforcement to the scaffold to make the structure resist to the physiological constraints applied during the regeneration represents a valid alternative. Silk fibroin is an interesting reinforcing candidate being a mechanically strong natural fibre, susceptible to proteolytic degradation in vivo and showing acceptable biological performances. Therefore, the aim of this study was to develop a model of a composite scaffold obtained by controlling the filament geometry winding of silk fibroin in the collagen gel. A finite element model taking into account the orthotropic elasticity of arteries has been combined with classic laminate theory applied to the filament winding of a tubular vessel. The design of the small structure susceptible to scaffold the vascular tissue regeneration was optimised by mean of an evolutive algorithm with the imperative to mimic the experimentally measured mechanical properties (compliance) of a native artery.

Performance of a porous composite scaffold containing silk fibroin: Applied research repair on oral jaw epithelial defects

2020 ◽  
Vol 10 (4) ◽  
pp. 490-502
Author(s):  
Huajun Zhu ◽  
Chunyu Qian ◽  
Wanshu Xiao ◽  
Qiang Zhang ◽  
Zili Ge
Keyword(s):  
Mechanical Properties ◽  
Contact Angle ◽  
Silk Fibroin ◽  
Composite Scaffold ◽  
Epithelial Tissue ◽  
Porous Composite ◽  
Clinical Value ◽  
Freeze Dried ◽  
Silk Fibroin Film ◽  
Oral Epithelial

Application research on repairing oral and maxillofacial epithelial defects with filin-protein porous composite scaffold. The silk fibroin solution was synthesized by hydrothermal synthesis, and the film was prepared by stirring and pouring. Then silk fibroin film and silk fibroin freeze-dried support were prepared by stirring and smooth casting. It was characterized by FTIR, mechanical properties, dissolution detection, contact Angle and SEM. To evaluate the performance of this material in repairing rabbit oral mucosa and rabbit skin epithelial defects. The characterization shows that the material has good contact Angle, mechanical properties, dissolution and biocompatibility. It has good repair function to rabbit oral epithelial tissue and skin epithelial tissue. Silk fibroin has excellent and unique properties. It has good development prospects and great clinical value in tissue regeneration.

scholarly journals Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

2020 ◽  
Vol 11 ◽  
pp. 204173142096779
Author(s):  
Ziquan Wu ◽  
Zhulong Meng ◽  
Qianjin Wu ◽  
Delu Zeng ◽  
Zhengdong Guo ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Bone Repair ◽  
Composite Scaffold ◽  
Bone Tissue Regeneration ◽  
Bone Mesenchymal Stem Cells ◽  
Differentiation Potential ◽  
Composite Effect ◽  
Sd Rat

Artificial bioactive materials have received increasing attention worldwide in clinical orthopedics to repair bone defects that are caused by trauma, infections or tumors, especially dedicated to the multifunctional composite effect of materials. In this study, a weakly alkaline, biomimetic and osteogenic, three-dimensional composite scaffold (3DS) with hydroxyapatite (HAp) and nano magnesium oxide (MgO) embedded in fiber (F) of silkworm cocoon and silk fibroin (SF) is evaluated comprehensively for its bone repair potential in vivo and in vitro experiments, particularly focusing on the combined effect between HAp and MgO. Magnesium ions (Mg2+) has long been proven to promote bone tissue regeneration, and HAp is provided with osteoconductive properties. Interestingly, the weak alkaline microenvironment from MgO may also be crucial to promote Sprague-Dawley (SD) rat bone mesenchymal stem cells (BMSCs) proliferation, osteogenic differentiation and alkaline phosphatase (ALP) activities. This SF/F/HAp/nano MgO (SFFHM) 3DS with superior biocompatibility and biodegradability has better mechanical properties, BMSCs proliferation ability, osteogenic activity and differentiation potential compared with the scaffolds adding HAp or MgO alone or neither. Similarly, corresponding meaningful results are also demonstrated in a model of distal lateral femoral defect in SD rat. Therefore, we provide a promising 3D composite scaffold for promoting bone regeneration applications in bone tissue engineering.

A poly(lactide-co-glycolide)/hydroxyapatite composite scaffold with enhanced osteoconductivity

2007 ◽  
Vol 80A (1) ◽  
pp. 206-215 ◽  
Author(s):  
Sang-Soo Kim ◽  
Kang-Min Ahn ◽  
Min Sun Park ◽  
Jong-Ho Lee ◽  
Cha Yong Choi ◽  
...  
Keyword(s):  
Composite Scaffold ◽  
Hydroxyapatite Composite

Biodegradation of Multilayer Silk Fibroin and Hydroxyapatite Composite Material

2006 ◽  
Vol 309-311 ◽  
pp. 1169-1172 ◽  
Author(s):  
Rikako Kino ◽  
Toshiyuki Ikoma ◽  
Shunji Yunoki ◽  
Akira Monkawa ◽  
Atsushi Matsuda ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Body Fluid ◽  
Multilayer Film ◽  
Compression Method ◽  
Sample Weight ◽  
Hydroxyapatite Composite ◽  
Phosphate Buffered Saline ◽  
Β Sheet ◽  
Film Method

Silk fibroin (SF) films containing 5wt% of CaCl2 were prepared by a cast-film method from the degummed SF and then immersed into the simulated body fluid (SBF) to deposit hydroxyapatite (HAp) crystals. The multilayer film of HAp and SF (5-layers), and pure SF film (4-layers) were prepared by a thermo-compression method at 130 °C and 3MPa for 4min. The ratio of β-sheet structure against other structures in both samples showed almost same value of 55.8% and 55.1%. The swelling ratio and in vitro biodegradation were examined by incubating in phosphate-buffered saline (PBS) with and without protease XIV for 1 to 14 days. The changes of sample weight and its tensile strength were investigated. The multilayer film showed slower biodegradation and higher mechanical strength compared with pure SF film.

Sign in / Sign up

Export Citation Format

Share Document