Performance of a porous composite scaffold containing silk fibroin: Applied research repair on oral jaw epithelial defects

2020 ◽  
Vol 10 (4) ◽  
pp. 490-502
Author(s):  
Huajun Zhu ◽  
Chunyu Qian ◽  
Wanshu Xiao ◽  
Qiang Zhang ◽  
Zili Ge
Keyword(s):  
Mechanical Properties ◽  
Contact Angle ◽  
Silk Fibroin ◽  
Composite Scaffold ◽  
Epithelial Tissue ◽  
Porous Composite ◽  
Clinical Value ◽  
Freeze Dried ◽  
Silk Fibroin Film ◽  
Oral Epithelial

Application research on repairing oral and maxillofacial epithelial defects with filin-protein porous composite scaffold. The silk fibroin solution was synthesized by hydrothermal synthesis, and the film was prepared by stirring and pouring. Then silk fibroin film and silk fibroin freeze-dried support were prepared by stirring and smooth casting. It was characterized by FTIR, mechanical properties, dissolution detection, contact Angle and SEM. To evaluate the performance of this material in repairing rabbit oral mucosa and rabbit skin epithelial defects. The characterization shows that the material has good contact Angle, mechanical properties, dissolution and biocompatibility. It has good repair function to rabbit oral epithelial tissue and skin epithelial tissue. Silk fibroin has excellent and unique properties. It has good development prospects and great clinical value in tissue regeneration.

Design of a Collagen/Silk Mechano-Compatible Composite Scaffold for the Vascular Tissue Engineering: Focus on Compliance

2007 ◽  
Vol 334-335 ◽  
pp. 1169-1172 ◽  
Author(s):  
Frédéric Couet ◽  
Navneeta Rajan ◽  
Simone Vesentini ◽  
D. Mantovani
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Vascular Tissue ◽  
Composite Scaffold ◽  
Collagen Gel ◽  
Element Model ◽  
Natural Fibre ◽  
Filament Winding ◽  
Laminate Theory

One of the merging methods to produce tissue-engineered vascular substitutes is to process scaffolds to direct the regeneration of vascular tissues. Collagen, as one of the main protein in the vascular extracellular matrix, is one of biopolymers that exhibits a major potential for scaffold technology. However, gels made from reconstituted collagen generally exhibit poor mechanical properties and limited manipulability. Therefore, adding a reinforcement to the scaffold to make the structure resist to the physiological constraints applied during the regeneration represents a valid alternative. Silk fibroin is an interesting reinforcing candidate being a mechanically strong natural fibre, susceptible to proteolytic degradation in vivo and showing acceptable biological performances. Therefore, the aim of this study was to develop a model of a composite scaffold obtained by controlling the filament geometry winding of silk fibroin in the collagen gel. A finite element model taking into account the orthotropic elasticity of arteries has been combined with classic laminate theory applied to the filament winding of a tubular vessel. The design of the small structure susceptible to scaffold the vascular tissue regeneration was optimised by mean of an evolutive algorithm with the imperative to mimic the experimentally measured mechanical properties (compliance) of a native artery.

scholarly journals Biocomposites conductive scaffold based on PEDOT:PSS/nHA/chitosan/PCL: Fabrication and characterization

2019 ◽  
Vol 15 (2) ◽  
pp. 146-149
Author(s):  
Alireza Lari ◽  
Naznin Sultana ◽  
Chin Fhong Soon
Keyword(s):  
Mechanical Properties ◽  
Contact Angle ◽  
Composite Scaffold ◽  
Biological Evaluation ◽  
Sem Analysis ◽  
Human Skin Fibroblast ◽  
Fabrication Technique ◽  
Pore Sizes ◽  
Material Component

Biomaterial-based scaffolds with suitable characteristics are highly desired in tissue engineering (TE) application. Biocomposites based on polymer and ceramics increase the chance for modulating the properties of scaffold. In recent years, researchers have considered conductive polymers to be used in TE application, due to their conductivity. This property has a good impact on tissue regeneration. A suitable design for bone substitute that consists of considerations such as material component, fabrication technique and mechanical properties. The previous studies on PEDOT:PSS/nHA/CS showed high wettability rate but low mechanical properties. Polycaprolactone (PCL) is a biodegradable and biocompatible polymer with a low wettability. The incorporation of PCL inside biocomposite can lead to the decrement in wettability and increment in mechanical property. In addition, this paper would examine the feasibility of blending of PCL and chitosan to fabricate PEDOT:PSS/nHA/CS composite scaffold. The fabrication technique of freezing/ lyophilization was used in this study. The scaffolds were characterized morphologically using scanning electron microscopy (SEM). Wettability was studied using a contact angle instrument. The attenuated total reflectance fourier transform infrared spectroscopy (ATR-FTIR) spectra interpreted the presence of polymeric ingredients within composite scaffold. Conductivity of the scaffolds was measured using a Digital Multimeter. In-vitro biological evaluation of the scaffolds was studied using human skin Fibroblast (HSF) cell line. The morphological study of biocomposite PEDOT:PSS/nHA/CS/PCL scaffold revealed random pore sizes and 66% porosity. Contact angle of the scaffold was increased and the swelling property and pore sizes were decreased after blending of PCL polymer. The viability of HSF cells on biocomposite PEDOT:PSS/nHA/CS/PCL scaffold was 85%. After 7 days, SEM analysis revealed the presence of cells on the surface of scaffold. In conclusion, the results suggested that PEDOT:PSS/nHA/CS/PCL biocomposite scaffold was non-toxic to cells and has suitable properties.

Preparation and Characteristics of Gradient Silk Fibroin/Hydroxyapatite Porous Composites

2009 ◽  
Vol 610-613 ◽  
pp. 1231-1236
Author(s):  
Wei Wei Xu ◽  
Jian Bing Liu ◽  
Ming Zhong Li ◽  
Shen Zhou Lu ◽  
Miao Liang Luo
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Bending Strength ◽  
Bone Tissue Regeneration ◽  
Gradient Material ◽  
Layer By Layer ◽  
Porous Composite ◽  
Pressing Method ◽  
Linear Gradient ◽  
Porous Composites

The gradient silk fibroin (SF)/ hydroxyapatite (HA) porous composite used for the scaffold of cartilage-bone tissue regeneration was prepared with SF powder and HA powder by layer-by-layer mould pressing method and granular NaCl as porogent. The characteristics were performed by Electron Microprobe and Energy Dispersive Spectometer (EDS). It indicated that the distribution of SF and HA presented linear gradient along the thickness direction in the materials. The porosity of composites increased with NaCl content increasing, while the density decreased. As the result of mechanical properties measurement, the bending strength and compressive strength decreased with NaCl content increasing. Compared the mechanical properties between gradient material and non-gradient material, it indicated that bending strength of gradient SF/HA porous material was higher than non-gradient SF/HA porous material’s.

scholarly journals Studies of electrical, thermal, and mechanical properties of single-walled carbon nanotube and polyaniline of nanoporous nanocomposites

2021 ◽  
Vol 11 ◽  
pp. 184798042110011
Author(s):  
Parvathalu Kalakonda ◽  
Pranay Bhasker Kalakonda ◽  
Sreenivas Banne
Keyword(s):  
Mechanical Properties ◽  
Carbon Nanotubes ◽  
Carbon Nanotube ◽  
Composite Scaffold ◽  
Single Walled Carbon Nanotubes ◽  
Fabrication Method ◽  
Porous Composite ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

Hydrogel of single-walled carbon nanotubes and polyaniline has been used for thermopower engineering applications due to desirable thermal, electrical, and mechanical properties as well as tunable degradability. In this article, we fabricated nanoporous composite scaffolds from hydrogel of single-walled carbon nanotubes and polyaniline polymer using a standard in situ polymerization process. Our solution-based fabrication method prevented single-walled carbon nanotube aggregation which resulted in enhancing thermal, electrical, and mechanical properties with keeping optimum flexibility in the porous composite scaffold. We compared the mechanical, electrical, and thermal properties of nanoporous composites with different single-walled carbon nanotube loadings. The porous composite scaffold with a 25 wt% showed higher electrical conductivity, ultimate tensile strength, and tensile modulus. Lastly, our solution fabrication method prevents aggregation single-walled carbon nanotube and could help to build the thermoelectrical materials for flexible electronic applications.

Study on Hydroxylapatite Fibroin Porous Composite

2007 ◽  
Vol 330-332 ◽  
pp. 947-950
Author(s):  
Shen Zhou Lu ◽  
Ming Zhong Li ◽  
Lun Bai
Keyword(s):  
Mechanical Properties ◽  
Porous Materials ◽  
Pore Size ◽  
Silk Fibroin ◽  
Ultrasonic Vibration ◽  
Fiber Content ◽  
Complex Materials ◽  
Porous Composite ◽  
Gel Method ◽  
Breaking Energy

The complex porous materials, Hydroxyapatite(HA)/silk fibroin, were prepared using ultrasonic vibration gel method in this paper. The mechanical properties decreased with the increase of HA while the porosity increased. The pore size become bigger than 100μm when adding silk fiber in the complex materials, and the breaking energy increased evidently from 10 to 1000 J/m2 when fiber content increased from 0 to 4 wt.%. It was observed from SEM that all composites have interconnected micropores.

Manufacturing Porous Blocks of Nano-Composite of Needle-Like Hydroxyapatite Crystallites and Chitin for Tissue Engineering

2005 ◽  
Vol 288-289 ◽  
pp. 199-202 ◽  
Author(s):  
Qian Peng ◽  
Jie Weng ◽  
Xiao Hong Li ◽  
Zong Wei Gu
Keyword(s):  
Tissue Engineering ◽  
Composite Scaffold ◽  
Porous Composite ◽  
Nano Composite ◽  
Gel Casting ◽  
Composite Gel ◽  
Freeze Dried ◽  
Porous Blocks ◽  
Interconnected Pores

A porous composite scaffold for tissue engineering had been developed through a novel gel-casting approach with needle-like nano hydroxyapatite (HA) crystallites and chitin. The freeze-dried nano-HA powder was firstly dispersed in the chitin solvent before chitin dissolves in its solvent completely. The composite solution was evenly mixed with a selected porosifier and poured into a mold in which it became a composite gel under the reaction of water molecules in atmosphere. Subsequently, the composite gel was subjected to the simultaneous extraction of the porosifier and chitin solvent in distilled water. After the drying process the porous composite scaffold was obtained. The morphological analysis showed that the manufactured scaffold had uniform and isotropic porous structure with controlled, fully interconnected pores. In vitro experiments indicated that the bone-like apatite layer formed easily on the walls of the porous n-HA/chitin composite scaffold.

Preparation and properties of a biodegradable poly(lactide-co-glycolide)/poly(trimethylene carbonate) porous composite scaffold for bone tissue engineering

2020 ◽  
Vol 44 (34) ◽  
pp. 14632-14641 ◽  
Author(s):  
Jin Qi ◽  
Yu Zhang ◽  
Xiliang Liu ◽  
Qianmao Zhang ◽  
Chengdong Xiong
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Porous Scaffold ◽  
Composite Scaffold ◽  
High Porosity ◽  
Trimethylene Carbonate ◽  
Porous Composite ◽  
Biodegradable Poly ◽  
Good Biocompatibility ◽  
Interconnected Pore

New biodegradable PLGA/PTMC composite porous scaffold with high porosity, mechanical properties, significant homogeneous, interconnected pore network and good biocompatibility.

The Progress in Research on Silk Fibroin Blend Membranes

2011 ◽  
Vol 311-313 ◽  
pp. 2052-2058 ◽  
Author(s):  
Xiao Xu Xu ◽  
Yong Peng Yu ◽  
Li Qun Yang
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Synthetic Polymers ◽  
Blend Film ◽  
The Poor ◽  
Biological Compatibility ◽  
Silk Fibroin Film ◽  
Blend Membranes ◽  
Medical Materials

Silk fibroin film, which has good biological compatibility, is suitable for use as biomaterials and medical materials. However, silk fibroin film in dry state is too brittle to be used by itself. Blending with other natural or synthetic polymers could improve the poor mechanical properties of silk fibroin film. In this paper, recent researches in blend film of silk fibroin and their application were summarized.

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