Increased wheat protein content via introgression of a HMW glutenin selectively reshapes the grain proteome

Abstract Improvement of grain protein content (GPC), loaf volume and resistance to rusts was achieved in 11 Indian wheat cultivars that are widely grown in four different agro-climatic zones of India. This involved use of marker-assisted backcrossing (MABC) for introgression and pyramiding of the following genes: (i) the high GPC gene Gpc-B1; (ii) HMW glutenin subunits 5 + 10 at Glu-D1 loci, and (iii) rust resistance genes, Yr36, Yr15, Lr24 and Sr24. GPC was improved by 0.8–3.3%, although high GPC was generally associated with yield penalty. Further selection among high GPC lines, allowed development of progenies with higher GPC associated with improvement in 1000-grain weight and grain yield in the following four cultivars: NI5439, UP2338, UP2382 and HUW468. The high GPC progenies (derived from NI5439) were also improved for grain quality using HMW glutenin subunits 5 + 10 at Glu-D1 loci. Similarly, progenies combining high GPC and rust resistance were developed in the backgrounds of following five cultivars: Lok1, HD2967, PBW550, PBW621 and DBW1. The improved pre-bred lines developed during the present study should prove useful for development of cultivars with improved nutritional quality associated with rust resistance in future wheat breeding programmes.

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VARIATION IN BREADMAKING QUALITY OF SYSTEMATIC CONTROLS IN A WHEAT BREEDING NURSERY AND ITS RELATIONSHIP TO PLANT BREEDING PROCEDURES

Canadian Journal of Plant Science ◽

10.4141/cjps69-002 ◽

1969 ◽

Vol 49 (1) ◽

pp. 21-28 ◽

Cited By ~ 4

Author(s):

K. G. Briggs ◽

W. Bushuk ◽

L. H. Shebeski

Keyword(s):

Protein Content ◽

Wheat Breeding ◽

Quality Data ◽

Wheat Protein ◽

Quality Test ◽

Breadmaking Quality ◽

Test Characteristics ◽

Moisture Basis ◽

Flour Yield ◽

The University

In a spring wheat breeding nursery at the University of Manitoba in 1967, the wheat protein content of systematic control plots of Triticum aestivum cv. Manitou varied from 10.3% to 16.5% (at 13.5% moisture basis). The correlation between grain yield and protein content of these plots was 0.88 and significant at the 99% confidence level. Correlations calculated for control plots at specified distances apart indicate that for all breadmaking quality test characteristics except bushel weight and flour yield, contiguous plots are significantly more similar in quality than those further apart. The correlation between control plots 2.7 m (9 ft) apart is 0.84 (P = 0.05) for wheat protein percent and of similar order for those quality characteristics which are dependent on total protein. Areas of high and low quality "potential" can be identified in a wheat nursery by using quality data from controls at frequent intervals, and this information should be used by the breeder when assessing the single quality test of a breeding line from a given area of the nursery.

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SUMMERFALLOW WHEAT PROTEIN VARIATIONS IN SASKATCHEWAN

Canadian Journal of Soil Science ◽

10.4141/cjss64-027 ◽

1964 ◽

Vol 44 (2) ◽

pp. 196-202 ◽

Cited By ~ 1

Author(s):

R. B. McKercher

Keyword(s):

Protein Content ◽

Soil Profile ◽

Field Studies ◽

Measured Data ◽

Wheat Protein

Field studies were conducted during the years 1957 to 1962 inclusive to measure and delineate factors which cause the protein variations encountered in summerfallow wheat crops in Saskatchewan. Differences in protein content of wheat of over 7% were found between different sampling topographical positions within any one field. Fertilization with nitrogen and phosphate at recommended field rates did not alter the protein content of the grain even though increases in yield of up to 100% were measured. Data are presented which indicate that much of the variation in protein values of wheat within fields can be attributed to changes in the type of soil profile and the associated microclimate.

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EFFECTS OF FOLIAR- AND SOIL-APPLIED NITROGEN AND SOIL NITRATE-NITROGEN LEVEL ON THE PROTEIN CONTENT OF NEEPAWA WHEAT

Canadian Journal of Soil Science ◽

10.4141/cjss72-042 ◽

1972 ◽

Vol 52 (3) ◽

pp. 301-309 ◽

Cited By ~ 14

Author(s):

A. C. ALKIER ◽

G. J. RACZ ◽

R. J. SOPER

Keyword(s):

Ammonium Nitrate ◽

Protein Content ◽

Nitrate Nitrogen ◽

Field Studies ◽

Nitrogen Supply ◽

Soil Nitrate ◽

Fallow Land ◽

Wheat Protein ◽

Large Yield ◽

Ha Protein

Protein content and yield of wheat usually increased with increases in the amount of nitrate nitrogen present in the soil. Large yield increases were obtained when 34 or 67 kg N/ha was applied broadcast to nonfallow land at time of seeding, but protein content decreased or increased only slightly. Protein content increased substantially when 101–202 kg N/ha was applied to nonfallow land, whereas yields were not increased above that obtained with 67 kg N/ha. Protein content of wheat grown on fallow land increased with increasing amounts of added nitrogen up to about 134 kg N/ha; yields did not increase greatly with added nitrogen. Wheat protein content was found to be related to nitrogen supply. An R2 value of 0.78 was obtained when wheat protein content was related to soil nitrate nitrogen measured to a depth of 61 cm and nitrogen applied broadcast at time of seeding. Soil- and foliar-applied postemergent nitrogen applications increased the protein content more than equivalent rates of nitrogen-applied broadcast at time of seeding in field studies conducted during 1971, but not in 1970. A greenhouse experiment showed that usually less than 1% of the nitrogen from ammonium nitrate, urea, or ammonium sulfate was absorbed into the grain when applied to the foliar surfaces of wheat; usually greater than 30% of the nitrogen from these fertilizers was absorbed when soil applied.

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Influence of irrigation and nitrogen fertilization on the amino acid composition of spring wheat

Agricultural and Food Science ◽

10.23986/afsci.71810 ◽

1972 ◽

Vol 44 (1) ◽

pp. 56-62

Author(s):

Paavo Elonen ◽

Liisa Aho ◽

Pekka Koivistoinen

Keyword(s):

Amino Acid ◽

Amino Acid Composition ◽

Protein Content ◽

Acid Composition ◽

Wheat Flour ◽

Spring Wheat ◽

Nitrogen Fertilization ◽

Field Experiments ◽

Sprinkler Irrigation ◽

Wheat Protein

Sprinkler irrigation on clay soils in southern Finland decreased the protein content of spring wheat flour in five field experiments in the years 1967—70, by 16 ± 4 per cent on an average. The amino acid composition, however, was improved, because the proportion of lysine was increased by 6 ± 4 %. Accordingly, the content of lysine in flour was decreased by irrigation only by 10 ± 5 %. An increase of fertilizer nitrogen from 68 to 144 kg/ha increased the protein content of wheat flour by 19 ± 5 %, but the proportion of lysine in the amino acid composition decreased by 9 ± 4 %. Therefore, the lysine content in flour was increased by nitrogen fertilization only by 8 ± 5 %. It seemed as if urea and ammonium nitrate limestone would have had about the same effect on the quantity and quality of wheat protein. As a consequence of the reversed influences of irrigation and nitrogen fertilization on the wheat protein it was possible, by means of irrigation and an additional nitrogen fertilization, to produce 65 per cent higher grain yields without any noteworthy changes in the contents of protein or amino acids.

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