Virulence factor genes and comparative pathogenicity study of tdh, trh and tlh positive Vibrio parahaemolyticus strains isolated from Whiteleg shrimp, Litopenaeus vannamei (Boone, 1931) in India

2021 ◽  
pp. 105083
Author(s):  
Prasenjit Paria ◽  
Bijay Kumar Behera ◽  
Pradeep Kumar Das Mohapatra ◽  
Pranaya Kumar Parida
Keyword(s):  
Virulence Factor ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Whiteleg Shrimp ◽  
Virulence Factor Genes

scholarly journals ANTIBACTERIAL ACTIVITY OF HERBAL EXTRACTS AGAINST Vibrio parahaemolyticus CAUSING ACUTE HEPATOPANCREATIC NECROSIS DISEASE (AHPND) IN WHITELEG SHRIMP (Litopenaeus vannamei) IN VITRO

2020 ◽  
Vol 1 (38) ◽  
pp. 82-88
Author(s):  
Nguyen Thi Nguyen ◽  
Nhi Thi Hong Nguyen
Keyword(s):  
Antibacterial Activity ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Leaf Extract ◽  
Herbal Extracts ◽  
Physalis Angulata ◽  
Whiteleg Shrimp ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Muntingia Calabura

The antibacterial activity of herbal extracts to Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in whiteleg shrimp (Litopenaeus vannamei) was studied and carried out in the School of Agriculture and Aquaculture, Tra Vinh University. The results showed that the inhibition zone of Muntingia calaburaleaf extract with ethanol 70% was 18.00 ± 0.00mm, Muntingia calabura leaf extract with ethanol 90% was 17.33 ± 0.58 mm and Muntingia calabura fresh leaf extract was 13.00 ± 0.00 mm. Minimum inhibitory concentration (MIC) of Muntingia calabura leaf extract with ethanol 70% and 90% were 5.120 mg/L, 10.240 mg/L. MIC value of Al lium cepa extract with ethanol 70% and 90% were 40.960 mg/L, Physalis angulata extract with ethanol 70% and 90% were 81.920 mg/L. The results showed that the antimicrobial activity was highest in the Muntingiacalabura leaf extract and lowest in Physalis angulata extract.

scholarly journals Phycoerythrin from Colaconema sp. Has Immunostimulatory Effects on the Whiteleg Shrimp Litopenaeus vannamei and Increases Resistance to Vibrio parahaemolyticus and White Spot Syndrome Virus

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2371
Author(s):  
Po-Tsang Lee ◽  
Jing Huang ◽  
Chin-Yi Huang ◽  
Zi-Xuan Liu ◽  
Han-Yang Yeh ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
White Spot Syndrome Virus ◽  
Expression Profiles ◽  
White Spot ◽  
Shrimp Culture ◽  
White Spot Syndrome ◽  
Whiteleg Shrimp

We investigated whether phycoerythrin (PE), a pigment sourced from marine algae, could act as an immunomodulatory agent in whiteleg shrimp (Litopenaeus vannamei). To this end, PE was extracted and purified from a PE-rich macroalgae, Colaconema sp. Our in vitro analysis demonstrated that PE enhanced prophenoloxidase and phagocytosis activity but inhibited the production of reactive oxygen species in hemocytes. Additionally, the PE signal could be detected using an in vivo imaging system after its injection into the ventral sinus of the cephalothorax of whiteleg shrimp. The expression profiles of fourteen immune-related genes were monitored in hemocytes from whiteleg shrimp injected with 0.30 μg of PE per gram of body weight, and crustin, lysozyme, penaiedin 4, and anti-lipopolysaccharide factor showed up-regulated post-stimulation. The induction of immune genes and enhancement of innate immune parameters by PE may explain the higher survival rates for shrimp that received different doses of PE prior to being challenged with Vibrio parahaemolyticus or white spot syndrome virus compared to controls. Combined, these results show that PE from Colaconema sp. can differentially stimulate the immune response of whiteleg shrimp in vitro and in vivo and could potentially be used as an immunomodulator in shrimp culture.

scholarly journals Prevalensi Vibrio parahaemolyticus pada Udang Vaname di Unit Pengolahan Ikan Jawa Tengah dan Jawa Timur

2020 ◽  
Vol 15 (1) ◽  
pp. 21
Author(s):  
Arifah Kusmarwati ◽  
Fairdiana Andayani ◽  
Yusma Yennie
Keyword(s):  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Foodborne Disease ◽  
Fish Processing ◽  
Post Harvest ◽  
Central Java ◽  
Pathogenic Vibrio ◽  
Processing Step ◽  
Whiteleg Shrimp

Vibrio parahaemolyticus patogen selain membahayakan kesehatan manusia juga menjadi alasan penolakan ekspor udang di beberapa negara. Penelitian ini dilakukan untuk mengisolasi, mengidentifikasi dan mengkuantifikasi keberadaan V. parahaemolyticus patogen pada udang vaname (Litopenaeus vannamei) di Unit Pengolahan Ikan (UPI) Jawa Tengah dan Jawa Timur. Pengambilan sampel udang vaname dilakukan secara tertelusur di sepanjang rantai pasok pengolahan udang menggunakan metode acak purposif. Sampel udang yang diambil berukuran 20-48 g/ekor (size 21-51). Sebanyak 10 unit sampel dengan berat masing-masing 400-500 g diambil dari setiap tambak udang intensif. Dengan berat yang sama, sebanyak 10-30 sampel diambil dari setiap tahapan pengolahan udang di UPI secara tertelusur. Identifikasi V. parahaemolyticus merujuk pada metode SNI 01-2332.5-2006 dan PCR dengan gen penyandi spesifik toxR, tdh dan trh. Prevalensi V. parahaemolyticus dari total sampel udang yang berasal dari tambak dan UPI Jawa Tengah adalah 17 dari 50 (34%) sampel, di mana empat (4) sampel positif mengandung gen tdh dan tiga (3)  sampel positif mengandung gen trh. Sedangkan prevalensi V. parahaemolyticus dari sampel yang berasal dari Jawa Timur sebesar 29 dari 60 (48,33%) sampel positif PCR, dan tidak terdeteksi V. parahaemolyticus patogen. Oleh karena itu sangat penting dilakukan upaya perbaikan penanganan udang paska panen, khususnya di UPI Jawa Tengah terutama pada titik kritis proses pengolahannya.    AbstractApart from being a major cause of foodborne disease in human, pathogenic Vibrio parahaemolyticus also becomes the main reason for shrimp export rejection in several countries. This study was carried out to isolate, identify and quantify the prevalence of pathogenic V. parahaemolyticus in whiteleg shrimp (Litopenaeus vannamei). Shrimp with size of 21-51 (20-48 g/shrimp) from ponds and fish processing units in Central and East Java were used in this study. Ten sample units of 400-500 g shrimp were collected from each intensive pond, while 10-30 sample units (400-500 g) were collected from each processing step in the fish processing units. Pathogenic V. parahaemolyticus was identified using PCR with specific coding gen (toxR, tdh and trh) and method recommended by SNI 01-2332.5-2006. The prevalence of V. parahaemolyticus in shrimp from Central Java was 17 out of 50 (34%) samples. Amongst these, four (4) were positive tdh and three (3) were positive trh. For sample from East Java, V. parahaemolyticus was found 29 out of 60 (48.33%) and no pathogenic V. parahaemolyticus was detected. To avoid future contamination of V. parahaemolyticus, post-harvest handling of shrimp especially in the processing units in Central Java should be improved.

scholarly journals Draft Genome Sequence of Bowmanella denitrificans JL63, a Bacterium Isolated from Whiteleg Shrimp ( Litopenaeus vannamei ) That Can Inhibit the Growth of Vibrio parahaemolyticus

2018 ◽  
Vol 6 (14) ◽  
Author(s):  
Jason P. LaPorte ◽  
Edward J. Spinard ◽  
Marta Gomez-Chiarri ◽  
David C. Rowley ◽  
John J. Mekalanos ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Genome Sequence ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Whiteleg Shrimp ◽  
Acute Hepatopancreatic Necrosis Disease

ABSTRACT Bowmanella denitrificans strain JL63 was isolated from a whiteleg shrimp ( Litopenaeus vannamei ) and was determined to have antibacterial activity against an acute hepatopancreatic necrosis disease (AHPND) strain of Vibrio parahaemolyticus . Here, we report the draft genome sequence of this strain and identify genes that are potentially involved in its antibacterial activity.

Label-free proteomic analysis revealed the mechanisms of protein oxidation induced by hydroxyl radicals in whiteleg shrimp (Litopenaeus vannamei) muscle

2021 ◽  
Author(s):  
Hui-min Lin ◽  
Xue-er Qi ◽  
Shan-shan Shui ◽  
Soottawat Benjakul ◽  
Santiago P. Aubourg ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Hydroxyl Radicals ◽  
Litopenaeus Vannamei ◽  
Protein Oxidation ◽  
Proteomic Analysis ◽  
Muscle Proteins ◽  
Label Free ◽  
Whiteleg Shrimp ◽  
The Stability ◽  
Oxidative Effects

The oxidative effects of hydroxyl radicals derived from a FeCl3/ascorbic acid/H2O2 system on the stability of muscle proteins in peeled shrimp (Litopenaeus vannamei) were investigated.

Sign in / Sign up

Export Citation Format

Share Document